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The map label for this gene is hpaI [H]

Identifier: 15603400

GI number: 15603400

Start: 1733695

End: 1734495

Strand: Direct

Name: hpaI [H]

Synonym: PM1535

Alternate gene names: 15603400

Gene position: 1733695-1734495 (Clockwise)

Preceding gene: 15603399

Following gene: 15603401

Centisome position: 76.8

GC content: 46.32

Gene sequence:

>801_bases
CTGAAAAATCACTTTAAACAAGCGTTAAAAGAGAAAAAACCGCAAATTGGCTTATGGGTCGGCTTAGCGGATGGCTATGC
TTGTGAGCTTGCCGCCACTGCCGGATTTGATTGGTTATTGCTCGATGGTGAGCATGCCCCGAATGATCTACGCACCTTGT
TACATCAGCTCCAAACCTTAGCCGCGTATCCTACTACTCCGATTATTCGCCCCGTGATTGGACAAACGCATACCATTAAA
CAACTGCTTGATATTGGGGCGCAGACCTTATTGGTCCCGATGGTGGAAACCGCTGAACAAGCGCGCGAATTGGTGAAAGC
TATACATTATCCACCAAAAGGTGTGCGTGGCGTAGGCAGTGCCTTAGCGCGTGCGTCGCGTTGGAATAGTATTCCTCATT
ATCTACAACAAGCGGATGAAGAAATCTGTTTATTGGTGCAAGTGGAAAACAAAACCGGCTTACAGAACCTGAAAGAGATT
GCTGAAGTCGATGGGGTCGATGGCGTTTTTATTGGACCTGCCGATCTGAGTGCGTCCTTAGGGCATTTAGGCAATCCATC
GCATCCAGAAGTGCAACAAGCGATTGAACAAGCGATTGCCACCATTTGTGCAGCCGATAAAGCTGCCGGTATTTTGTATG
CCGATGAAAAAATGGCAAAACACTATTTAGCACTCGGCTGTACGTTTGTGGCGGTAGGCGTCGATACCTCTTTGTTAATG
CGTGCATTAAACGATCTTTCACAGAAGTTTAAGTCATCGAATGCACTAGAAAACACAGCTAGTGCGAACCAAATTTATTA
A

Upstream 100 bases:

>100_bases
GTCGCCGCCCGTCGTGGTGATACATTCCATGTGGATTACCATGAATTAGGCAGTATTTCGATGCAGTTTGTGTAATGGCA
ACGTGAGGCGTACAATGCAA

Downstream 100 bases:

>100_bases
TTGTTGGGAGGTATTTCAATGCAACAATTTTCCCTATTAAAAACCAAAGCCTATATTAATGGCGAGTATGTACAGGCAAA
GGAGCACAAAACCTTTGCGG

Product: HpaI

Products: NA

Alternate protein names: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase; HHED aldolase; 4-hydroxy-2-ketoheptane-1,7-dioate aldolase; HKHD aldolase [H]

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MKNHFKQALKEKKPQIGLWVGLADGYACELAATAGFDWLLLDGEHAPNDLRTLLHQLQTLAAYPTTPIIRPVIGQTHTIK
QLLDIGAQTLLVPMVETAEQARELVKAIHYPPKGVRGVGSALARASRWNSIPHYLQQADEEICLLVQVENKTGLQNLKEI
AEVDGVDGVFIGPADLSASLGHLGNPSHPEVQQAIEQAIATICAADKAAGILYADEKMAKHYLALGCTFVAVGVDTSLLM
RALNDLSQKFKSSNALENTASANQIY

Sequences:

>Translated_266_residues
MKNHFKQALKEKKPQIGLWVGLADGYACELAATAGFDWLLLDGEHAPNDLRTLLHQLQTLAAYPTTPIIRPVIGQTHTIK
QLLDIGAQTLLVPMVETAEQARELVKAIHYPPKGVRGVGSALARASRWNSIPHYLQQADEEICLLVQVENKTGLQNLKEI
AEVDGVDGVFIGPADLSASLGHLGNPSHPEVQQAIEQAIATICAADKAAGILYADEKMAKHYLALGCTFVAVGVDTSLLM
RALNDLSQKFKSSNALENTASANQIY
>Mature_266_residues
MKNHFKQALKEKKPQIGLWVGLADGYACELAATAGFDWLLLDGEHAPNDLRTLLHQLQTLAAYPTTPIIRPVIGQTHTIK
QLLDIGAQTLLVPMVETAEQARELVKAIHYPPKGVRGVGSALARASRWNSIPHYLQQADEEICLLVQVENKTGLQNLKEI
AEVDGVDGVFIGPADLSASLGHLGNPSHPEVQQAIEQAIATICAADKAAGILYADEKMAKHYLALGCTFVAVGVDTSLLM
RALNDLSQKFKSSNALENTASANQIY

Specific function: Catalyzes the reversible retro-aldol cleavage of 4- hydroxy-2-ketoheptane-1,7-dioate (HKHD) to pyruvate and succinic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788578, Length=252, Percent_Identity=57.5396825396825, Blast_Score=296, Evalue=1e-81,
Organism=Escherichia coli, GI1789514, Length=251, Percent_Identity=45.0199203187251, Blast_Score=229, Evalue=1e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR012689
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.2.-

Molecular weight: Translated: 28749; Mature: 28749

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNHFKQALKEKKPQIGLWVGLADGYACELAATAGFDWLLLDGEHAPNDLRTLLHQLQTL
CCHHHHHHHHHCCCCEEEEEEECCCCEEEEHHHCCCEEEEECCCCCCHHHHHHHHHHHHH
AAYPTTPIIRPVIGQTHTIKQLLDIGAQTLLVPMVETAEQARELVKAIHYPPKGVRGVGS
HHCCCCCHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
ALARASRWNSIPHYLQQADEEICLLVQVENKTGLQNLKEIAEVDGVDGVFIGPADLSASL
HHHHHHHCCCHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEECCHHHHHHH
GHLGNPSHPEVQQAIEQAIATICAADKAAGILYADEKMAKHYLALGCTFVAVGVDTSLLM
HCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHCCHHHHH
RALNDLSQKFKSSNALENTASANQIY
HHHHHHHHHHHCCCHHHHCCCCCCCC
>Mature Secondary Structure
MKNHFKQALKEKKPQIGLWVGLADGYACELAATAGFDWLLLDGEHAPNDLRTLLHQLQTL
CCHHHHHHHHHCCCCEEEEEEECCCCEEEEHHHCCCEEEEECCCCCCHHHHHHHHHHHHH
AAYPTTPIIRPVIGQTHTIKQLLDIGAQTLLVPMVETAEQARELVKAIHYPPKGVRGVGS
HHCCCCCHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
ALARASRWNSIPHYLQQADEEICLLVQVENKTGLQNLKEIAEVDGVDGVFIGPADLSASL
HHHHHHHCCCHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEECCHHHHHHH
GHLGNPSHPEVQQAIEQAIATICAADKAAGILYADEKMAKHYLALGCTFVAVGVDTSLLM
HCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHCCHHHHH
RALNDLSQKFKSSNALENTASANQIY
HHHHHHHHHHHCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA