Click here to switch to the map view.

The map label for this gene is carA

Identifier: 15603367

GI number: 15603367

Start: 1698343

End: 1699500

Strand: Direct

Name: carA

Synonym: PM1502

Alternate gene names: 15603367

Gene position: 1698343-1699500 (Clockwise)

Preceding gene: 15603364

Following gene: 15603368

Centisome position: 75.23

GC content: 42.14

Gene sequence:

>1158_bases
ATGTCTGAACCAGCAATTTTGGTATTGGCTGATGGGAGTGTTTTCCATGGCACCTCTATTGGAGCAAAAGGCCACACCGT
AGGCGAAGTGGTGTTTAATACCGCTATGACAGGCTATCAGGAAATTCTAACGGACCCTTCTTATTTTCGACAAATCGTCA
CCTTAACTTATCCTCACATCGGCAATACCGGTACAAATCTAGAAGATTGCGAAGCGAATCACGTTTATGCCTCAGGGCTG
ATTATTCGCGATTTACCCTTATTACACAGCAATTTCCGTTCATCCATGAGTTTGCGTGATTATCTTCAAACCCATAATGT
GGTTGCTATTGCGGATATCGACACACGCCGTTTAACCCGTATTTTACGTGATAAAGGTGCGCAAGCGGGTTGTATCATGA
CCGGTGAGATTGATGAAGCCAAAGCCCTTGAGTTAGCGAAAAGTTTTGGGTCTATGGCGGGCAAAGATCTTGCGCAAGAA
GTGAGTACGGGTGAAATTTTTACTTGGACATCAGGTCAATGGCAATTGGGTAAAGGGTTTATTGAACAAACCCAAGCGGA
ATTTAATGTGGTGGCTTATGACTTTGGTGTCAAACACAATATTTTAAGAATGCTCGCTGAGCGTGGTTGCAAAATCACCG
TTGTACCGGCAAAAACCAGTGCAGAGCAAGTGTTAGCATTAAATCCAGATGGCATTTTCTTGTCTAATGGACCAGGCGAT
CCGGAACCTTGTGATTATGCCATTTCCGCTATTCAAACCTTATTAGCCTCAAAGAAACCCATTTTTGGTATTTGTTTAGG
TCATCAGTTATTAGGTTTAGCCGCAGGTGGTAAAACTAAAAAAATGGCATTTGGGCATCATGGTGCTAATCACCCCGTAC
AAGATTTGAATACACAAAAAGTGTTTATCACGAGTCAAAATCATGGTTTTGAAGTAGATGAAGCTAGCTTACCAAGTCAT
GTGCGTGTGACACATCGTTCATTATTTGACAATTCTGTACAGGGTATTGAACTCAGTGATCAGTCTGCGTTTTCTTTCCA
AGGGCACCCTGAGGCGAGTCCAGGTCCAAATGATGTGGCTTATTTATTTGATAAATTTATTAATGAAATGCGCAAGGCAA
ATTTAAGTCAGTTATCGACTTATGTCGCGCACCATTAG

Upstream 100 bases:

>100_bases
TCAGCGAAACCCGAAAGCGCGGTGAATTTTCGCAAACTTTACTAGATTGTGGTTAATGAACATTCGTAAGTGTTCAACCA
AAACAAATTAGGAGTAATTT

Downstream 100 bases:

>100_bases
TGAGGCTATGCGTAGGTTGAGGTAGTACTACGCCTTATTTATTAAATCATGACCGCATTTTTATTCAACTTTTGAGGCTC
GATGATGTTAACCCCTTATG

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MSEPAILVLADGSVFHGTSIGAKGHTVGEVVFNTAMTGYQEILTDPSYFRQIVTLTYPHIGNTGTNLEDCEANHVYASGL
IIRDLPLLHSNFRSSMSLRDYLQTHNVVAIADIDTRRLTRILRDKGAQAGCIMTGEIDEAKALELAKSFGSMAGKDLAQE
VSTGEIFTWTSGQWQLGKGFIEQTQAEFNVVAYDFGVKHNILRMLAERGCKITVVPAKTSAEQVLALNPDGIFLSNGPGD
PEPCDYAISAIQTLLASKKPIFGICLGHQLLGLAAGGKTKKMAFGHHGANHPVQDLNTQKVFITSQNHGFEVDEASLPSH
VRVTHRSLFDNSVQGIELSDQSAFSFQGHPEASPGPNDVAYLFDKFINEMRKANLSQLSTYVAHH

Sequences:

>Translated_385_residues
MSEPAILVLADGSVFHGTSIGAKGHTVGEVVFNTAMTGYQEILTDPSYFRQIVTLTYPHIGNTGTNLEDCEANHVYASGL
IIRDLPLLHSNFRSSMSLRDYLQTHNVVAIADIDTRRLTRILRDKGAQAGCIMTGEIDEAKALELAKSFGSMAGKDLAQE
VSTGEIFTWTSGQWQLGKGFIEQTQAEFNVVAYDFGVKHNILRMLAERGCKITVVPAKTSAEQVLALNPDGIFLSNGPGD
PEPCDYAISAIQTLLASKKPIFGICLGHQLLGLAAGGKTKKMAFGHHGANHPVQDLNTQKVFITSQNHGFEVDEASLPSH
VRVTHRSLFDNSVQGIELSDQSAFSFQGHPEASPGPNDVAYLFDKFINEMRKANLSQLSTYVAHH
>Mature_384_residues
SEPAILVLADGSVFHGTSIGAKGHTVGEVVFNTAMTGYQEILTDPSYFRQIVTLTYPHIGNTGTNLEDCEANHVYASGLI
IRDLPLLHSNFRSSMSLRDYLQTHNVVAIADIDTRRLTRILRDKGAQAGCIMTGEIDEAKALELAKSFGSMAGKDLAQEV
STGEIFTWTSGQWQLGKGFIEQTQAEFNVVAYDFGVKHNILRMLAERGCKITVVPAKTSAEQVLALNPDGIFLSNGPGDP
EPCDYAISAIQTLLASKKPIFGICLGHQLLGLAAGGKTKKMAFGHHGANHPVQDLNTQKVFITSQNHGFEVDEASLPSHV
RVTHRSLFDNSVQGIELSDQSAFSFQGHPEASPGPNDVAYLFDKFINEMRKANLSQLSTYVAHH

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI18105007, Length=381, Percent_Identity=36.2204724409449, Blast_Score=231, Evalue=8e-61,
Organism=Homo sapiens, GI21361331, Length=394, Percent_Identity=35.0253807106599, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI169790915, Length=394, Percent_Identity=35.0253807106599, Blast_Score=223, Evalue=2e-58,
Organism=Escherichia coli, GI1786215, Length=371, Percent_Identity=65.4986522911051, Blast_Score=514, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI193204318, Length=383, Percent_Identity=37.0757180156658, Blast_Score=237, Evalue=9e-63,
Organism=Saccharomyces cerevisiae, GI6324878, Length=386, Percent_Identity=38.0829015544041, Blast_Score=233, Evalue=3e-62,
Organism=Saccharomyces cerevisiae, GI6322331, Length=403, Percent_Identity=35.4838709677419, Blast_Score=223, Evalue=3e-59,
Organism=Drosophila melanogaster, GI45555749, Length=394, Percent_Identity=37.5634517766497, Blast_Score=216, Evalue=2e-56,
Organism=Drosophila melanogaster, GI24642586, Length=394, Percent_Identity=37.5634517766497, Blast_Score=216, Evalue=3e-56,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CARA_PASMU (Q9CKV3)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246441.1
- ProteinModelPortal:   Q9CKV3
- SMR:   Q9CKV3
- GeneID:   1244849
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1502
- NMPDR:   fig|272843.1.peg.1503
- HOGENOM:   HBG286341
- OMA:   IMTAPHI
- ProtClustDB:   PRK12564
- BioCyc:   PMUL272843:PM1502-MONOMER
- BRENDA:   6.3.5.5
- HAMAP:   MF_01209_B
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- PANTHER:   PTHR11405:SF4
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR01368

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase; SSF52021 CP_synthsmall

EC number: =6.3.5.5

Molecular weight: Translated: 41855; Mature: 41724

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 265-265 ACT_SITE 349-349 ACT_SITE 351-351

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPAILVLADGSVFHGTSIGAKGHTVGEVVFNTAMTGYQEILTDPSYFRQIVTLTYPHI
CCCCEEEEEECCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
GNTGTNLEDCEANHVYASGLIIRDLPLLHSNFRSSMSLRDYLQTHNVVAIADIDTRRLTR
CCCCCCCCCCCCCEEEECCEEEECCHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHH
ILRDKGAQAGCIMTGEIDEAKALELAKSFGSMAGKDLAQEVSTGEIFTWTSGQWQLGKGF
HHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEECCHHH
IEQTQAEFNVVAYDFGVKHNILRMLAERGCKITVVPAKTSAEQVLALNPDGIFLSNGPGD
HHHHCCCEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCEEEEECCCCEEECCCCCC
PEPCDYAISAIQTLLASKKPIFGICLGHQLLGLAAGGKTKKMAFGHHGANHPVQDLNTQK
CCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCCEEEECCCCCCCCHHCCCCCE
VFITSQNHGFEVDEASLPSHVRVTHRSLFDNSVQGIELSDQSAFSFQGHPEASPGPNDVA
EEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCEEECCCCCCCCCCCHHH
YLFDKFINEMRKANLSQLSTYVAHH
HHHHHHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure 
SEPAILVLADGSVFHGTSIGAKGHTVGEVVFNTAMTGYQEILTDPSYFRQIVTLTYPHI
CCCEEEEEECCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
GNTGTNLEDCEANHVYASGLIIRDLPLLHSNFRSSMSLRDYLQTHNVVAIADIDTRRLTR
CCCCCCCCCCCCCEEEECCEEEECCHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHH
ILRDKGAQAGCIMTGEIDEAKALELAKSFGSMAGKDLAQEVSTGEIFTWTSGQWQLGKGF
HHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEECCHHH
IEQTQAEFNVVAYDFGVKHNILRMLAERGCKITVVPAKTSAEQVLALNPDGIFLSNGPGD
HHHHCCCEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCEEEEECCCCEEECCCCCC
PEPCDYAISAIQTLLASKKPIFGICLGHQLLGLAAGGKTKKMAFGHHGANHPVQDLNTQK
CCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCCEEEECCCCCCCCHHCCCCCE
VFITSQNHGFEVDEASLPSHVRVTHRSLFDNSVQGIELSDQSAFSFQGHPEASPGPNDVA
EEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCEEECCCCCCCCCCCHHH
YLFDKFINEMRKANLSQLSTYVAHH
HHHHHHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100