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The map label for this gene is ribB

Identifier: 15603250

GI number: 15603250

Start: 1581294

End: 1581938

Strand: Reverse

Name: ribB

Synonym: PM1385

Alternate gene names: 15603250

Gene position: 1581938-1581294 (Counterclockwise)

Preceding gene: 15603251

Following gene: 15603248

Centisome position: 70.08

GC content: 42.02

Gene sequence:

>645_bases
ATGAATCAGTCTATTTTATCTCCTTTTGGTCAGTCAGAAGAACGTGCTATTGCTGCGATTAATGCGTTTAAACAGGGAAA
CGGTGTTTTAGTTCTTGATGATGAAGATCGTGAAAACGAAGGTGATCTCATTTTTCCTGCTGAAACCATTACAGTAGAAC
AAATGACAATGTTAATTCGTTATGGCAGTGGTATCGTTTGTTTATGTTTAAGCGATGAAATTTGCCAACAACTTGATTTG
CCACCGATGGTTACTGAAAATACCAGTGTGAATAAGACGGCCTTTACGGTGACGATTGAGGCAGCTAAAGGTGTATCGAC
AGGCGTATCCGCCACGGATCGTGTGACAACTATTCGTGCTGCCGTTGCCGATAATGCCAAACCAAGCGATTTAAGCCGTC
CGGGACATATTTTTCCATTACGTGCGATGACAGGTGGCGTGTTAAAACGCCGTGGGCATACTGAAGCATCAGTTGATCTT
GCTCGTTTAGCTGGTTATAAAGCCGCTGGTGTGATTTGTGAAATTACCAATGATGATGGTTCAATGGCTCGTGCACCAGA
AATTGTTACGTTTGCTCAGAAATTTGGTTTTCCAGTCGTTACGATTGAAGATTTAGTGGCTTATCGTCAAAAGTATGATG
TGTAA

Upstream 100 bases:

>100_bases
ACCTGTATTTTTTGTTTGTTTAAGATTTTCTTAGCCACATACTTTTCTCATCAGCCCTGATTCTGGTATCTAAATATCTT
TATATTTCAAGGAATTTACT

Downstream 100 bases:

>100_bases
TATTTAGTGTGTATAAAAAAGTGCGGTCAAAATTTTATCTGACCGCACTTTTTGTATTAGGATAATTAATTCGTTTTCAG
CTTGTTCCAATATTTTTCAT

Product: 3,4-dihydroxy-2-butanone 4-phosphate synthase

Products: NA

Alternate protein names: DHBP synthase

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MNQSILSPFGQSEERAIAAINAFKQGNGVLVLDDEDRENEGDLIFPAETITVEQMTMLIRYGSGIVCLCLSDEICQQLDL
PPMVTENTSVNKTAFTVTIEAAKGVSTGVSATDRVTTIRAAVADNAKPSDLSRPGHIFPLRAMTGGVLKRRGHTEASVDL
ARLAGYKAAGVICEITNDDGSMARAPEIVTFAQKFGFPVVTIEDLVAYRQKYDV

Sequences:

>Translated_214_residues
MNQSILSPFGQSEERAIAAINAFKQGNGVLVLDDEDRENEGDLIFPAETITVEQMTMLIRYGSGIVCLCLSDEICQQLDL
PPMVTENTSVNKTAFTVTIEAAKGVSTGVSATDRVTTIRAAVADNAKPSDLSRPGHIFPLRAMTGGVLKRRGHTEASVDL
ARLAGYKAAGVICEITNDDGSMARAPEIVTFAQKFGFPVVTIEDLVAYRQKYDV
>Mature_214_residues
MNQSILSPFGQSEERAIAAINAFKQGNGVLVLDDEDRENEGDLIFPAETITVEQMTMLIRYGSGIVCLCLSDEICQQLDL
PPMVTENTSVNKTAFTVTIEAAKGVSTGVSATDRVTTIRAAVADNAKPSDLSRPGHIFPLRAMTGGVLKRRGHTEASVDL
ARLAGYKAAGVICEITNDDGSMARAPEIVTFAQKFGFPVVTIEDLVAYRQKYDV

Specific function: Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate

COG id: COG0108

COG function: function code H; 3,4-dihydroxy-2-butanone 4-phosphate synthase

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHBP synthase family

Homologues:

Organism=Escherichia coli, GI1789420, Length=213, Percent_Identity=67.1361502347418, Blast_Score=305, Evalue=1e-84,
Organism=Saccharomyces cerevisiae, GI6320695, Length=201, Percent_Identity=45.771144278607, Blast_Score=182, Evalue=5e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIBB_PASMU (P57940)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246324.1
- ProteinModelPortal:   P57940
- SMR:   P57940
- GeneID:   1244732
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1385
- NMPDR:   fig|272843.1.peg.1386
- HOGENOM:   HBG735778
- OMA:   RGHTEAT
- ProtClustDB:   PRK01792
- BioCyc:   PMUL272843:PM1385-MONOMER
- BRENDA:   4.1.99.12
- HAMAP:   MF_00180
- InterPro:   IPR017945
- InterPro:   IPR000422
- Gene3D:   G3DSA:3.90.870.10
- TIGRFAMs:   TIGR00506

Pfam domain/function: PF00926 DHBP_synthase; SSF55821 DHBP_synth_RibB-like_a/b_dom

EC number: =4.1.99.12

Molecular weight: Translated: 23042; Mature: 23042

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: BINDING 42-42 BINDING 174-174

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQSILSPFGQSEERAIAAINAFKQGNGVLVLDDEDRENEGDLIFPAETITVEQMTMLIR
CCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEECCHHHHHHHHHEEHH
YGSGIVCLCLSDEICQQLDLPPMVTENTSVNKTAFTVTIEAAKGVSTGVSATDRVTTIRA
CCCCEEEEEECHHHHHHCCCCCCEECCCCCCCEEEEEEEEECCCCCCCCCCCHHHHEEEE
AVADNAKPSDLSRPGHIFPLRAMTGGVLKRRGHTEASVDLARLAGYKAAGVICEITNDDG
HHCCCCCCCCCCCCCCEEEHHHHHCHHHHHCCCCHHHHHHHHHCCCCCCCEEEEEECCCC
SMARAPEIVTFAQKFGFPVVTIEDLVAYRQKYDV
CCCCCCHHHHHHHHHCCCEEEHHHHHHHHHHCCC
>Mature Secondary Structure
MNQSILSPFGQSEERAIAAINAFKQGNGVLVLDDEDRENEGDLIFPAETITVEQMTMLIR
CCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEECCHHHHHHHHHEEHH
YGSGIVCLCLSDEICQQLDLPPMVTENTSVNKTAFTVTIEAAKGVSTGVSATDRVTTIRA
CCCCEEEEEECHHHHHHCCCCCCEECCCCCCCEEEEEEEEECCCCCCCCCCCHHHHEEEE
AVADNAKPSDLSRPGHIFPLRAMTGGVLKRRGHTEASVDLARLAGYKAAGVICEITNDDG
HHCCCCCCCCCCCCCCEEEHHHHHCHHHHHCCCCHHHHHHHHHCCCCCCCEEEEEECCCC
SMARAPEIVTFAQKFGFPVVTIEDLVAYRQKYDV
CCCCCCHHHHHHHHHCCCEEEHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11248100