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The map label for this gene is galE [C]

Identifier: 15603225

GI number: 15603225

Start: 1550830

End: 1551777

Strand: Reverse

Name: galE [C]

Synonym: PM1360

Alternate gene names: 15603225

Gene position: 1551777-1550830 (Counterclockwise)

Preceding gene: 15603226

Following gene: 15603224

Centisome position: 68.74

GC content: 41.67

Gene sequence:

>948_bases
ATGAAAATTATCATTACGGGCGGGCAAGGATTCCTTGGACAGCGTTTAGCAAAAACCTTGTTATCCCATCCACATGTACA
AGTCGATGAGCTGATTTTAATTGATGTGATTAAGCCTATCGCACCCAACCATGATCCCAGAGTACGTTGTATAGAAATGG
ACTTACGCCAACCAGAAGGATTGGATGAGATTATTGATCAGCAGACAGATGCCATTTTCCATTTAGCCGCGATTGTCAGT
AGCCATGCGGAACAAGATCCTGATTTAGGCTATGAGATTAATTTCTTAGCGACGCGTCATTTACTCGAATGTTGCCGTCA
TCGTAATCCAAAAATTCGTTTTATTTTTACCAGCTCATTAGCTGTCTATGGAGGAAAGCTACCTGACGTTATTGAGGATC
ATACTGCCGTCACACCACAATCCACATATGGTACACAAAAAGCCATGTGTGAGTTATTGATTAATGATTATAGCCGCAAA
GGGTTTGTGGATGGCATTGTGCTTCGCTTGCCAACCATTTGTATTCGTCCGGGTAAACCGAATAAAGCTGCTTCTTCATT
TGTGAGCAGTATCATTCGTGAGCCGTTGCATAACGAAATGGCAATTTGCCCGGTGTCTGTGCAATTAAAATTATGGCTAT
CAAGTCCAAATACAGTCGTGAATAATTTTATTCACGCACTTCAGCTACCTTCTCTCCCTGCAAGAGATTGGCATGTCATC
AATTTACCAGGCTTTACTGTGACTGTGGAAAACATGTTGTCTGAGTTAGCTAAGGTAAAAGGGGATGCGATTTTACAGTA
TGTCAATGTGGCGTTTGACGAAGGGATTAACAATATTGTTGCCAGTTGGCCTGCGGCAATTAACTGTTCACACGCACTTG
GTTTAGGTTTCCATGCCGATCAACGTTTTGCGGATGTGATTCAACAATTCATTCAATATGATATGTAA

Upstream 100 bases:

>100_bases
AAAATGCCCTGTTGTCAGTGGGTAGGGCATTTCTTACATAACAACCCTTAAACTGCATGAAATTTGAACAAAAGTGCGGT
GAATTTTTGAGGAGAAAAAG

Downstream 100 bases:

>100_bases
CGGGAGGCGTTATGTTTGGATTACCTATTCCAATAATCGGCTTATTAGTGGCCGTTTTTGTGCTCGTCTTTTTAGTGTTA
AGAACGCGCGTTCATGCTTT

Product: hypothetical protein

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MKIIITGGQGFLGQRLAKTLLSHPHVQVDELILIDVIKPIAPNHDPRVRCIEMDLRQPEGLDEIIDQQTDAIFHLAAIVS
SHAEQDPDLGYEINFLATRHLLECCRHRNPKIRFIFTSSLAVYGGKLPDVIEDHTAVTPQSTYGTQKAMCELLINDYSRK
GFVDGIVLRLPTICIRPGKPNKAASSFVSSIIREPLHNEMAICPVSVQLKLWLSSPNTVVNNFIHALQLPSLPARDWHVI
NLPGFTVTVENMLSELAKVKGDAILQYVNVAFDEGINNIVASWPAAINCSHALGLGFHADQRFADVIQQFIQYDM

Sequences:

>Translated_315_residues
MKIIITGGQGFLGQRLAKTLLSHPHVQVDELILIDVIKPIAPNHDPRVRCIEMDLRQPEGLDEIIDQQTDAIFHLAAIVS
SHAEQDPDLGYEINFLATRHLLECCRHRNPKIRFIFTSSLAVYGGKLPDVIEDHTAVTPQSTYGTQKAMCELLINDYSRK
GFVDGIVLRLPTICIRPGKPNKAASSFVSSIIREPLHNEMAICPVSVQLKLWLSSPNTVVNNFIHALQLPSLPARDWHVI
NLPGFTVTVENMLSELAKVKGDAILQYVNVAFDEGINNIVASWPAAINCSHALGLGFHADQRFADVIQQFIQYDM
>Mature_315_residues
MKIIITGGQGFLGQRLAKTLLSHPHVQVDELILIDVIKPIAPNHDPRVRCIEMDLRQPEGLDEIIDQQTDAIFHLAAIVS
SHAEQDPDLGYEINFLATRHLLECCRHRNPKIRFIFTSSLAVYGGKLPDVIEDHTAVTPQSTYGTQKAMCELLINDYSRK
GFVDGIVLRLPTICIRPGKPNKAASSFVSSIIREPLHNEMAICPVSVQLKLWLSSPNTVVNNFIHALQLPSLPARDWHVI
NLPGFTVTVENMLSELAKVKGDAILQYVNVAFDEGINNIVASWPAAINCSHALGLGFHADQRFADVIQQFIQYDM

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786974, Length=170, Percent_Identity=31.1764705882353, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24667531, Length=296, Percent_Identity=24.3243243243243, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 35054; Mature: 35054

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIITGGQGFLGQRLAKTLLSHPHVQVDELILIDVIKPIAPNHDPRVRCIEMDLRQPEG
CEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCC
LDEIIDQQTDAIFHLAAIVSSHAEQDPDLGYEINFLATRHLLECCRHRNPKIRFIFTSSL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCCCEEEEEEECCH
AVYGGKLPDVIEDHTAVTPQSTYGTQKAMCELLINDYSRKGFVDGIVLRLPTICIRPGKP
HHHCCCCCHHHHCCCCCCCCHHCCHHHHHHHHHHHHHCCCCHHHHHHHHCCEEEECCCCC
NKAASSFVSSIIREPLHNEMAICPVSVQLKLWLSSPNTVVNNFIHALQLPSLPARDWHVI
CHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEEECCCHHHHHHHHHHHCCCCCCCCCEEEE
NLPGFTVTVENMLSELAKVKGDAILQYVNVAFDEGINNIVASWPAAINCSHALGLGFHAD
ECCCCEEEHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCH
QRFADVIQQFIQYDM
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MKIIITGGQGFLGQRLAKTLLSHPHVQVDELILIDVIKPIAPNHDPRVRCIEMDLRQPEG
CEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCC
LDEIIDQQTDAIFHLAAIVSSHAEQDPDLGYEINFLATRHLLECCRHRNPKIRFIFTSSL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCCCEEEEEEECCH
AVYGGKLPDVIEDHTAVTPQSTYGTQKAMCELLINDYSRKGFVDGIVLRLPTICIRPGKP
HHHCCCCCHHHHCCCCCCCCHHCCHHHHHHHHHHHHHCCCCHHHHHHHHCCEEEECCCCC
NKAASSFVSSIIREPLHNEMAICPVSVQLKLWLSSPNTVVNNFIHALQLPSLPARDWHVI
CHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEEECCCHHHHHHHHHHHCCCCCCCCCEEEE
NLPGFTVTVENMLSELAKVKGDAILQYVNVAFDEGINNIVASWPAAINCSHALGLGFHAD
ECCCCEEEHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCH
QRFADVIQQFIQYDM
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]