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The map label for this gene is fusA [H]

Identifier: 15603221

GI number: 15603221

Start: 1544478

End: 1546580

Strand: Direct

Name: fusA [H]

Synonym: PM1356

Alternate gene names: 15603221

Gene position: 1544478-1546580 (Clockwise)

Preceding gene: 15603220

Following gene: 15603222

Centisome position: 68.42

GC content: 42.56

Gene sequence:

>2103_bases
ATGGCACGTACAACCCCTATTGAAAGATACCGTAACATTGGTATCAGTGCTCACATTGACGCAGGTAAAACAACCACTTC
CGAGCGTATCCTATTCTATACAGGTGTGAGTCACAAACTTGGTGAGGTGCACGATGGTGCAGCTACTATGGACTGGATGG
AGCAAGAACAAGAGCGTGGTATCACAATCACCTCTGCGGCAACAACCGCATTCTGGTCTGGTATGTCTAAACAATATCCA
CAACACCGTATCAACGTGATTGATACTCCGGGACACGTTGACTTCACGATCGAAGTTGAGCGTTCAATGCGTGTACTTGA
CGGTGCGGTGATGGTTTACTGTGCGGTGGGTGGTGTTCAACCACAATCAGAAACCGTATGGCGCCAAGCAAACAAATACC
AAGTTCCTCGTGTTGCTTTCGTAAACAAAATGGACCGTACTGGTGCTAACTTCTTACGTGTAGTTGAACAAATTAAAACT
CGCTTAGGGGGTAACTCTGTTCCTTTACAATTACCAATCGGTTCAGAAGATAATTTCAAAGGTGTTGTTGACCTTGTTAA
AATGAAAGCGATTAACTGGAACGAAGCCGATCAAGGTATGACATTTACTTATGAAGAGATCCCAGCTGATATGGTTGATG
CTTGTGAAGAATGGCGTCAAAATTTAGTTGAGTCAGCTGCAGAAGCGTCAGAAGAGTTAATGGAAAAATACCTTGGCGGT
GAAGAATTAACAGAAGAAGAAATTAAAGCAGGTTTACGTCAACGCGTATTAGCCGGTGAAATTATTCCTGTATGCTGTGG
TTCTGCCTTCAAAAATAAAGGTGTTCAAGCTATGCTTGATGCGGTAATCGACTATTTACCAGCACCAACAGATATCCCAG
CGATCAAAGGGATTAATCCAGATGAAACAGAAGGTGAGCGTCATGCAAGTGATGATGAACCTTTCTCTGCACTTGCGTTT
AAAATCGCAACTGACCCATTCGTAGGTAACTTAACGTTCTTCCGTGTTTATTCTGGTGTAATTAATTCAGGTGATACTGT
ATTAAACTCTGTAAAAGATAAACGTGAGCGTTTTGGTCGTATCGTACAGATGCACGCAAACAAACGTGAAGAAATCAAAG
AAGTACGTGCAGGCGATATCGCAGCCGCAATTGGTTTGAAAGATGTAGGTACTGGTGATACATTATGTGCACAAGATGCG
CCAATTATTCTTGAGCGCATGGAATTCCCAGAGCCAGTAATTTCTGTTGCTGTTGAGCCAAAAACGAAAGCAGACCAAGA
AAAAATGGGTCTTGCATTAGGTCGCTTAGCACAAGAAGACCCTTCATTCCGTGTTCATACTGATGAAGAGTCGGGTGAAA
CTATTATCTCTGGTATGGGTGAGTTACACTTAGATATTATCGTTGACCGTATGCGTCGTGAATTCAAAGTTGAAGCGAAC
ATTGGTAAACCACAAGTATCTTACCGTGAAACTATCCGTACTCGCGTTAATGATGTTGAAGGTAAACACGCAAAACAATC
TGGTGGTCGCGGTCAATATGGTCACGTTGTTATCGACTTATATCCGTTAGATCCAGAAGGTCCGGGTTATGAATTCGTGA
ACGAAATCAAAGGTGGTGTGATCCCTGGTGAATACATCCCTGCAGTAGATAAAGGTGTTCAAGAACAACTTAAATCGGGT
CCATTAGCGGGTTATCCAGTAGTGGATTTAGGTGTTCGTTTACACTTCGGTTCATACCATGATGTTGACTCATCTGAGCT
TGCGTTTAAATTAGCTGCATCTTTAGCCTTTAAAGCTGCATTTAATAAAGCAAACCCAGTGCTTCTTGAGCCAATCATGA
AAGTAGAAGTTGAAACGCCACCGGACTACGTTGGTGATGTAATTGGTGACTTAAGCCGTCGTCGTGCGATGGTTAACGGT
CAAGAAGCAAATGAATTTGTTGTGAAGATCAATGCAGAAGTACCACTTTCAGAGATGTTTGGTTATGCAACTGACCTTCG
TTCACAAACTCAAGGTCGTGCTTCATACTCAATGGAACCATTGAAATATGCAGAAGCGCCGAAAAACGTTGCTGATGCAA
TCATTGAAGCTCGCAAAAAATAA

Upstream 100 bases:

>100_bases
TTTTTAGCAACTACGTTTTCAGGCTTCATCTCATTTAATTATGTGGTGAAGCCTTATACATATTTAAAATTTCATATTTA
ATTCCAGCAAGGAAATAATA

Downstream 100 bases:

>100_bases
TTTTGTTTAACAAAACCACAAGCTGTAATAGCTTGTGGTCTCTACTGAGGAAACTTAATCGTGTCTAAAGAAAAATTTGA
ACGTACAAAACCGCACGTAA

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: EF-G [H]

Number of amino acids: Translated: 700; Mature: 699

Protein sequence:

>700_residues
MARTTPIERYRNIGISAHIDAGKTTTSERILFYTGVSHKLGEVHDGAATMDWMEQEQERGITITSAATTAFWSGMSKQYP
QHRINVIDTPGHVDFTIEVERSMRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRVAFVNKMDRTGANFLRVVEQIKT
RLGGNSVPLQLPIGSEDNFKGVVDLVKMKAINWNEADQGMTFTYEEIPADMVDACEEWRQNLVESAAEASEELMEKYLGG
EELTEEEIKAGLRQRVLAGEIIPVCCGSAFKNKGVQAMLDAVIDYLPAPTDIPAIKGINPDETEGERHASDDEPFSALAF
KIATDPFVGNLTFFRVYSGVINSGDTVLNSVKDKRERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVGTGDTLCAQDA
PIILERMEFPEPVISVAVEPKTKADQEKMGLALGRLAQEDPSFRVHTDEESGETIISGMGELHLDIIVDRMRREFKVEAN
IGKPQVSYRETIRTRVNDVEGKHAKQSGGRGQYGHVVIDLYPLDPEGPGYEFVNEIKGGVIPGEYIPAVDKGVQEQLKSG
PLAGYPVVDLGVRLHFGSYHDVDSSELAFKLAASLAFKAAFNKANPVLLEPIMKVEVETPPDYVGDVIGDLSRRRAMVNG
QEANEFVVKINAEVPLSEMFGYATDLRSQTQGRASYSMEPLKYAEAPKNVADAIIEARKK

Sequences:

>Translated_700_residues
MARTTPIERYRNIGISAHIDAGKTTTSERILFYTGVSHKLGEVHDGAATMDWMEQEQERGITITSAATTAFWSGMSKQYP
QHRINVIDTPGHVDFTIEVERSMRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRVAFVNKMDRTGANFLRVVEQIKT
RLGGNSVPLQLPIGSEDNFKGVVDLVKMKAINWNEADQGMTFTYEEIPADMVDACEEWRQNLVESAAEASEELMEKYLGG
EELTEEEIKAGLRQRVLAGEIIPVCCGSAFKNKGVQAMLDAVIDYLPAPTDIPAIKGINPDETEGERHASDDEPFSALAF
KIATDPFVGNLTFFRVYSGVINSGDTVLNSVKDKRERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVGTGDTLCAQDA
PIILERMEFPEPVISVAVEPKTKADQEKMGLALGRLAQEDPSFRVHTDEESGETIISGMGELHLDIIVDRMRREFKVEAN
IGKPQVSYRETIRTRVNDVEGKHAKQSGGRGQYGHVVIDLYPLDPEGPGYEFVNEIKGGVIPGEYIPAVDKGVQEQLKSG
PLAGYPVVDLGVRLHFGSYHDVDSSELAFKLAASLAFKAAFNKANPVLLEPIMKVEVETPPDYVGDVIGDLSRRRAMVNG
QEANEFVVKINAEVPLSEMFGYATDLRSQTQGRASYSMEPLKYAEAPKNVADAIIEARKK
>Mature_699_residues
ARTTPIERYRNIGISAHIDAGKTTTSERILFYTGVSHKLGEVHDGAATMDWMEQEQERGITITSAATTAFWSGMSKQYPQ
HRINVIDTPGHVDFTIEVERSMRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRVAFVNKMDRTGANFLRVVEQIKTR
LGGNSVPLQLPIGSEDNFKGVVDLVKMKAINWNEADQGMTFTYEEIPADMVDACEEWRQNLVESAAEASEELMEKYLGGE
ELTEEEIKAGLRQRVLAGEIIPVCCGSAFKNKGVQAMLDAVIDYLPAPTDIPAIKGINPDETEGERHASDDEPFSALAFK
IATDPFVGNLTFFRVYSGVINSGDTVLNSVKDKRERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVGTGDTLCAQDAP
IILERMEFPEPVISVAVEPKTKADQEKMGLALGRLAQEDPSFRVHTDEESGETIISGMGELHLDIIVDRMRREFKVEANI
GKPQVSYRETIRTRVNDVEGKHAKQSGGRGQYGHVVIDLYPLDPEGPGYEFVNEIKGGVIPGEYIPAVDKGVQEQLKSGP
LAGYPVVDLGVRLHFGSYHDVDSSELAFKLAASLAFKAAFNKANPVLLEPIMKVEVETPPDYVGDVIGDLSRRRAMVNGQ
EANEFVVKINAEVPLSEMFGYATDLRSQTQGRASYSMEPLKYAEAPKNVADAIIEARKK

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=690, Percent_Identity=45.2173913043478, Blast_Score=579, Evalue=1e-165,
Organism=Homo sapiens, GI19923640, Length=721, Percent_Identity=37.8640776699029, Blast_Score=447, Evalue=1e-125,
Organism=Homo sapiens, GI25306287, Length=718, Percent_Identity=34.4011142061281, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI25306283, Length=457, Percent_Identity=41.1378555798687, Blast_Score=310, Evalue=2e-84,
Organism=Homo sapiens, GI4503483, Length=403, Percent_Identity=29.2803970223325, Blast_Score=106, Evalue=6e-23,
Organism=Homo sapiens, GI157426893, Length=154, Percent_Identity=36.3636363636364, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI94966754, Length=138, Percent_Identity=40.5797101449275, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI310132016, Length=120, Percent_Identity=40, Blast_Score=83, Evalue=7e-16,
Organism=Homo sapiens, GI310110807, Length=120, Percent_Identity=40, Blast_Score=83, Evalue=7e-16,
Organism=Homo sapiens, GI310123363, Length=120, Percent_Identity=40, Blast_Score=83, Evalue=7e-16,
Organism=Escherichia coli, GI1789738, Length=704, Percent_Identity=84.6590909090909, Blast_Score=1228, Evalue=0.0,
Organism=Escherichia coli, GI1790835, Length=495, Percent_Identity=26.8686868686869, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI48994988, Length=182, Percent_Identity=36.2637362637363, Blast_Score=106, Evalue=6e-24,
Organism=Escherichia coli, GI1788922, Length=157, Percent_Identity=36.9426751592357, Blast_Score=93, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17533571, Length=691, Percent_Identity=43.4153400868307, Blast_Score=551, Evalue=1e-157,
Organism=Caenorhabditis elegans, GI17556745, Length=732, Percent_Identity=29.5081967213115, Blast_Score=308, Evalue=4e-84,
Organism=Caenorhabditis elegans, GI17506493, Length=404, Percent_Identity=28.7128712871287, Blast_Score=108, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17557151, Length=166, Percent_Identity=36.144578313253, Blast_Score=92, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI71988819, Length=147, Percent_Identity=34.6938775510204, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71988811, Length=147, Percent_Identity=34.6938775510204, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323098, Length=699, Percent_Identity=44.3490701001431, Blast_Score=563, Evalue=1e-161,
Organism=Saccharomyces cerevisiae, GI6322359, Length=800, Percent_Identity=31.125, Blast_Score=377, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6324707, Length=407, Percent_Identity=28.7469287469287, Blast_Score=100, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6320593, Length=407, Percent_Identity=28.7469287469287, Blast_Score=100, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6323320, Length=155, Percent_Identity=35.4838709677419, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324166, Length=174, Percent_Identity=33.9080459770115, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24582462, Length=700, Percent_Identity=45.1428571428571, Blast_Score=594, Evalue=1e-170,
Organism=Drosophila melanogaster, GI221458488, Length=740, Percent_Identity=32.7027027027027, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24585711, Length=476, Percent_Identity=27.9411764705882, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24585713, Length=476, Percent_Identity=27.9411764705882, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24585709, Length=476, Percent_Identity=27.9411764705882, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI78706572, Length=158, Percent_Identity=37.9746835443038, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI28574573, Length=196, Percent_Identity=31.6326530612245, Blast_Score=90, Evalue=5e-18,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 77187; Mature: 77056

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARTTPIERYRNIGISAHIDAGKTTTSERILFYTGVSHKLGEVHDGAATMDWMEQEQERG
CCCCCHHHHHHCCCCEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCC
ITITSAATTAFWSGMSKQYPQHRINVIDTPGHVDFTIEVERSMRVLDGAVMVYCAVGGVQ
EEEEEHHHHHHHHCHHHCCCCCCCEEEECCCCEEEEEEECHHHHHHHHHHEEEEECCCCC
PQSETVWRQANKYQVPRVAFVNKMDRTGANFLRVVEQIKTRLGGNSVPLQLPIGSEDNFK
CCHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHH
GVVDLVKMKAINWNEADQGMTFTYEEIPADMVDACEEWRQNLVESAAEASEELMEKYLGG
HHHHHHHHHCCCCCCCCCCCEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EELTEEEIKAGLRQRVLAGEIIPVCCGSAFKNKGVQAMLDAVIDYLPAPTDIPAIKGINP
CCCCHHHHHHHHHHHHHHCCHHHHHCCCHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
DETEGERHASDDEPFSALAFKIATDPFVGNLTFFRVYSGVINSGDTVLNSVKDKRERFGR
CCCCCCCCCCCCCCHHHHEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
IVQMHANKREEIKEVRAGDIAAAIGLKDVGTGDTLCAQDAPIILERMEFPEPVISVAVEP
HHHHHCCCHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCCHHEEEECC
KTKADQEKMGLALGRLAQEDPSFRVHTDEESGETIISGMGELHLDIIVDRMRREFKVEAN
CCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHCCCHHHHHHHHHHHHHHEEEECC
IGKPQVSYRETIRTRVNDVEGKHAKQSGGRGQYGHVVIDLYPLDPEGPGYEFVNEIKGGV
CCCCCHHHHHHHHHHHHHCCCCHHHCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHCCCC
IPGEYIPAVDKGVQEQLKSGPLAGYPVVDLGVRLHFGSYHDVDSSELAFKLAASLAFKAA
CCCCCCCHHHHHHHHHHHCCCCCCCCCEEECEEEEECCCCCCCHHHHHHHHHHHHHHHHH
FNKANPVLLEPIMKVEVETPPDYVGDVIGDLSRRRAMVNGQEANEFVVKINAEVPLSEMF
HCCCCCCEECCHHEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHH
GYATDLRSQTQGRASYSMEPLKYAEAPKNVADAIIEARKK
HHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
ARTTPIERYRNIGISAHIDAGKTTTSERILFYTGVSHKLGEVHDGAATMDWMEQEQERG
CCCCHHHHHHCCCCEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCC
ITITSAATTAFWSGMSKQYPQHRINVIDTPGHVDFTIEVERSMRVLDGAVMVYCAVGGVQ
EEEEEHHHHHHHHCHHHCCCCCCCEEEECCCCEEEEEEECHHHHHHHHHHEEEEECCCCC
PQSETVWRQANKYQVPRVAFVNKMDRTGANFLRVVEQIKTRLGGNSVPLQLPIGSEDNFK
CCHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHH
GVVDLVKMKAINWNEADQGMTFTYEEIPADMVDACEEWRQNLVESAAEASEELMEKYLGG
HHHHHHHHHCCCCCCCCCCCEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EELTEEEIKAGLRQRVLAGEIIPVCCGSAFKNKGVQAMLDAVIDYLPAPTDIPAIKGINP
CCCCHHHHHHHHHHHHHHCCHHHHHCCCHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
DETEGERHASDDEPFSALAFKIATDPFVGNLTFFRVYSGVINSGDTVLNSVKDKRERFGR
CCCCCCCCCCCCCCHHHHEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
IVQMHANKREEIKEVRAGDIAAAIGLKDVGTGDTLCAQDAPIILERMEFPEPVISVAVEP
HHHHHCCCHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCCHHEEEECC
KTKADQEKMGLALGRLAQEDPSFRVHTDEESGETIISGMGELHLDIIVDRMRREFKVEAN
CCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHCCCHHHHHHHHHHHHHHEEEECC
IGKPQVSYRETIRTRVNDVEGKHAKQSGGRGQYGHVVIDLYPLDPEGPGYEFVNEIKGGV
CCCCCHHHHHHHHHHHHHCCCCHHHCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHCCCC
IPGEYIPAVDKGVQEQLKSGPLAGYPVVDLGVRLHFGSYHDVDSSELAFKLAASLAFKAA
CCCCCCCHHHHHHHHHHHCCCCCCCCCEEECEEEEECCCCCCCHHHHHHHHHHHHHHHHH
FNKANPVLLEPIMKVEVETPPDYVGDVIGDLSRRRAMVNGQEANEFVVKINAEVPLSEMF
HCCCCCCEECCHHEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHH
GYATDLRSQTQGRASYSMEPLKYAEAPKNVADAIIEARKK
HHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA