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The map label for this gene is yiaJ [H]

Identifier: 15603122

GI number: 15603122

Start: 1450536

End: 1451345

Strand: Direct

Name: yiaJ [H]

Synonym: PM1257

Alternate gene names: 15603122

Gene position: 1450536-1451345 (Clockwise)

Preceding gene: 15603108

Following gene: 15603133

Centisome position: 64.25

GC content: 40.12

Gene sequence:

>810_bases
ATGGATGTGATGAAAGAAGAGGATAAAACGAAGAAAGCAGGCAGTAATCAAAGTCTGATTAGGGGCTTACTCCTGATAGA
TATTTTGAGTAATTTTCCAAACGGTTGCCCACTCGCAAAATTATCAGAATTATCAAATTTGAATAAAAGTACTACACATC
GTATGTTGCAAACGTTACAGTCTTGTGGCTATGTAAAACCTGCAAATACGCTAGGGGCTTATCGCTTAACGACAAAATGT
TTAACTCTCGGGCAAAAAACGCTGAGTTCATTAAATATTTTGAATCTTACCGCACCTTATTTGGAGCAGTTGAATTTAGA
AACAGGGGAAACCGTTAATTTATCGACCCGTGATCATCACCATGCGGTCATGATTTATAAGTTAGAACCGACCACAGGTA
TGTTACGTACACGTGCCTATATTGGGCAGCGTTTAGAACTCTATTGTTCTGCCATGGGCAAAATTTTTATGGCGTATGAA
AAAGGGCATGCGCTAGATGATTATTGGGAAAAGAATCAAGCGACGATTCAGCAACTGACCGTGAATACGATTGTGACACA
AGAAGCAATGGCATTGGAACTGGCGAAAATTCGCGAATTAGGTTACGCCATGGATAATGAAGAAAATGAACGAGGTGTCA
CTTGCTTAGCATGCCCTGTGTTTGACATTCATGGCAATGTCACCTATTCGGTTTCTATTTCCTTATCTACCGCACGTTTG
AAACAAATTGGTCAAGACACGTTGTTGCGCCATTTAAAACAAACGACTCAAGCAATTTCACAAGAATTGGGTGCGGTTAT
GCCGAGTTAA

Upstream 100 bases:

>100_bases
ATGAGATAGGAATTTTTTGCGCGTTTTGTGATCAAGATCCCATTTTTATTTTTCCGATATCTATTTAGAATAGCGAAAGA
TAGAATCGATTAATTTTTGG

Downstream 100 bases:

>100_bases
TCGCATAAAAGTAAAAGTAAAAAACCTGTCAACACAGTGTGTATTGACAGGTTTTTGCTTTTTAGTCAGGTAGTGTAATG
CCTTAATGTCCTTTATGGCT

Product: hypothetical protein

Products: NA

Alternate protein names: YiaKLMNOPQRS operon repressor; YiaK-S operon repressor [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MDVMKEEDKTKKAGSNQSLIRGLLLIDILSNFPNGCPLAKLSELSNLNKSTTHRMLQTLQSCGYVKPANTLGAYRLTTKC
LTLGQKTLSSLNILNLTAPYLEQLNLETGETVNLSTRDHHHAVMIYKLEPTTGMLRTRAYIGQRLELYCSAMGKIFMAYE
KGHALDDYWEKNQATIQQLTVNTIVTQEAMALELAKIRELGYAMDNEENERGVTCLACPVFDIHGNVTYSVSISLSTARL
KQIGQDTLLRHLKQTTQAISQELGAVMPS

Sequences:

>Translated_269_residues
MDVMKEEDKTKKAGSNQSLIRGLLLIDILSNFPNGCPLAKLSELSNLNKSTTHRMLQTLQSCGYVKPANTLGAYRLTTKC
LTLGQKTLSSLNILNLTAPYLEQLNLETGETVNLSTRDHHHAVMIYKLEPTTGMLRTRAYIGQRLELYCSAMGKIFMAYE
KGHALDDYWEKNQATIQQLTVNTIVTQEAMALELAKIRELGYAMDNEENERGVTCLACPVFDIHGNVTYSVSISLSTARL
KQIGQDTLLRHLKQTTQAISQELGAVMPS
>Mature_269_residues
MDVMKEEDKTKKAGSNQSLIRGLLLIDILSNFPNGCPLAKLSELSNLNKSTTHRMLQTLQSCGYVKPANTLGAYRLTTKC
LTLGQKTLSSLNILNLTAPYLEQLNLETGETVNLSTRDHHHAVMIYKLEPTTGMLRTRAYIGQRLELYCSAMGKIFMAYE
KGHALDDYWEKNQATIQQLTVNTIVTQEAMALELAKIRELGYAMDNEENERGVTCLACPVFDIHGNVTYSVSISLSTARL
KQIGQDTLLRHLKQTTQAISQELGAVMPS

Specific function: Negatively controls the transcription of the yiaKLMNOPQRS operon, which may be involved in the utilization of 2,3-diketo-L-gulonate [H]

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1789999, Length=258, Percent_Identity=65.1162790697674, Blast_Score=348, Evalue=2e-97,
Organism=Escherichia coli, GI1786468, Length=250, Percent_Identity=34.4, Blast_Score=131, Evalue=4e-32,
Organism=Escherichia coli, GI87082359, Length=229, Percent_Identity=32.3144104803493, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1788131, Length=227, Percent_Identity=32.15859030837, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI1786715, Length=254, Percent_Identity=31.1023622047244, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1790752, Length=216, Percent_Identity=30.5555555555556, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1788581, Length=251, Percent_Identity=25.8964143426295, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI145693096, Length=208, Percent_Identity=28.3653846153846, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 29942; Mature: 29942

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVMKEEDKTKKAGSNQSLIRGLLLIDILSNFPNGCPLAKLSELSNLNKSTTHRMLQTLQ
CCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHH
SCGYVKPANTLGAYRLTTKCLTLGQKTLSSLNILNLTAPYLEQLNLETGETVNLSTRDHH
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHCCCCCCCEEEECCCCCC
HAVMIYKLEPTTGMLRTRAYIGQRLELYCSAMGKIFMAYEKGHALDDYWEKNQATIQQLT
EEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHH
VNTIVTQEAMALELAKIRELGYAMDNEENERGVTCLACPVFDIHGNVTYSVSISLSTARL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCEEEEEEEEEHHHHH
KQIGQDTLLRHLKQTTQAISQELGAVMPS
HHCCHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDVMKEEDKTKKAGSNQSLIRGLLLIDILSNFPNGCPLAKLSELSNLNKSTTHRMLQTLQ
CCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHH
SCGYVKPANTLGAYRLTTKCLTLGQKTLSSLNILNLTAPYLEQLNLETGETVNLSTRDHH
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHCCCCCCCEEEECCCCCC
HAVMIYKLEPTTGMLRTRAYIGQRLELYCSAMGKIFMAYEKGHALDDYWEKNQATIQQLT
EEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHH
VNTIVTQEAMALELAKIRELGYAMDNEENERGVTCLACPVFDIHGNVTYSVSISLSTARL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCEEEEEEEEEHHHHH
KQIGQDTLLRHLKQTTQAISQELGAVMPS
HHCCHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]