Click here to switch to the map view.

The map label for this gene is dnaG

Identifier: 15603105

GI number: 15603105

Start: 1430751

End: 1432499

Strand: Direct

Name: dnaG

Synonym: PM1240

Alternate gene names: 15603105

Gene position: 1430751-1432499 (Clockwise)

Preceding gene: 15603104

Following gene: 15603106

Centisome position: 63.38

GC content: 41.28

Gene sequence:

>1749_bases
ATGAAAGGCTCAATTCCACGCACATTTATTGATGATCTGTTAGCCAAGACAGATATTGTGGAGCTTGTCAATTCAAGAGT
GAAGCTCAAAAAAGCAGGGCGAGATTACCAAGCTTGCTGTCCATTCCATCACGAAAAGACGCCTTCTTTTACGGTTAGTC
AAAAAAAACAGTTTTATCATTGCTTTGGCTGTGGTGCACACGGTAATGCAATCTCGTTTTTAATGGAATATGACAAACTT
GAATTCGTCGAAGCCATTGAAGAATTAGCAGGCATGCTCGGTTTAGAGATTCCGCGTGAAAATAAACCGCACTTTCATGG
CAAACAAATTAATCTGCAAACTAAGCGTAATTTATATGAATTGATGCAAGAAATTGCCCAATTCTATCAACAACAACTCG
CACAACATATTCCTGCACAAAGTTATTTACAACAACGCGGTTTGTCTCCTGAGGTGATTTCTCGTTTTCAAATTGGTTTC
GTACCGAATAGTTTTGATGCAGTTTTACAACGTTTTGGGCAACAGAAAGAAGATCAACAAAAACTCTTCGATCTTGGTAT
GCTTTCCCGTAGCGAGCAAGGCAAGATCTACGATCGCTTCCGTCATCGGATCATGTTCCCGATTCGCGATCGACGTGGCA
AAACCATTGCGTTTGGCGGACGCGTGCTTGGTGATGAAAAACCTAAATATTTAAACTCACCAGAAAGCGCGACCTACCAT
AAAGGAAATGAACTATATGGTTTATATGAAGCCTTACAAGCCAATGAATCACCAGAGATGTTACTGGTTGTGGAAGGCTA
CATGGATGTGGTGGCATTAGCACAATTTGGTGTTGATTATGCCGTTGCCTCACTCGGAACAGCGACTACCGCAGAACAAA
TTCAACTCCTGTTTCGTTCTACGGAACAAGCAATTTGTTGTTATGATGGGGATCGCGCGGGACGAGAAGCAGCTTGGCGA
GCGTTTGAAAATGCCCTTCCTTATTTAGAAGACGGGCGTCAATTAAAATTTGTTTTTCTGCCTGATGGTGAAGACCCTGA
TTCATTTATTCGTCAGCATGGAAAAGCCAGCTTTGAACAATATATGCAAAAAGCCCTCTCGCTGAGTGAGTTTTTATTCA
CTAGCCTCGCGCCACAAGTGGATTTTTCCAGTAAAGAAGGGAAAACAAAATTAGCCGCATTGGCGGTGCCATTAATCAAA
AAAATCCCTGGCGATATGTTACGCTTATCTCTACGCAACACGCTCGCGCAAAAACTCGGCATTTTAGACCAAGCCCAATT
GGAAAGTCTGATTCCAAGCTATTCTGAAATGAAAATAACAGCCTCGCCTCAACCGGTCAAAAGAACGCCAATGCGTGTAT
TAATTGGTCTGTTATTGCAAAATCCAGAATTAGCCCAATTGGTACCAGACTTATCTCCCTTACGAACACTGAATGAACCA
GGGCTGGATTTATTGGAAAAACTGACCGCACTTTGTCAAGAAAAAGTGGGCATTACTACCGGTCAAATACTTGAATATTG
GCGTGATACTGAACACAGCAAAGCCCTTGAAATTTTAGCAAGTTGGAATCATTTAGTAGAAGACACACAAATTGAAGAAA
CGTTCAAAAGCACGCTACGCTATTTGTACTTTCAACTTATTGAACATGAAATCGATTTACTGATTGCCAAAGATCGCTCA
GAAGGGCTAAATATGAATGAGCGGAAAAAGTTGACACAATTATTAGTGAAAAAACAACAAAAGGCATAG

Upstream 100 bases:

>100_bases
TAAAAACTCGAGTTATACAAACCGTGAAACCGATAGGTATCACGGTTTTGTTTCATTCAATCGCGCCATATTTTTATGTT
AAAGCAGGGGCATATACTTG

Downstream 100 bases:

>100_bases
TTAATCTAGCATAACAGTGATAAAATCAGGCGTATTTTATCTTCATAACTACTCAAGCAAGGCGGATATTACATTATGGA
TCAAAATCCACAATCTCAAT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 582; Mature: 582

Protein sequence:

>582_residues
MKGSIPRTFIDDLLAKTDIVELVNSRVKLKKAGRDYQACCPFHHEKTPSFTVSQKKQFYHCFGCGAHGNAISFLMEYDKL
EFVEAIEELAGMLGLEIPRENKPHFHGKQINLQTKRNLYELMQEIAQFYQQQLAQHIPAQSYLQQRGLSPEVISRFQIGF
VPNSFDAVLQRFGQQKEDQQKLFDLGMLSRSEQGKIYDRFRHRIMFPIRDRRGKTIAFGGRVLGDEKPKYLNSPESATYH
KGNELYGLYEALQANESPEMLLVVEGYMDVVALAQFGVDYAVASLGTATTAEQIQLLFRSTEQAICCYDGDRAGREAAWR
AFENALPYLEDGRQLKFVFLPDGEDPDSFIRQHGKASFEQYMQKALSLSEFLFTSLAPQVDFSSKEGKTKLAALAVPLIK
KIPGDMLRLSLRNTLAQKLGILDQAQLESLIPSYSEMKITASPQPVKRTPMRVLIGLLLQNPELAQLVPDLSPLRTLNEP
GLDLLEKLTALCQEKVGITTGQILEYWRDTEHSKALEILASWNHLVEDTQIEETFKSTLRYLYFQLIEHEIDLLIAKDRS
EGLNMNERKKLTQLLVKKQQKA

Sequences:

>Translated_582_residues
MKGSIPRTFIDDLLAKTDIVELVNSRVKLKKAGRDYQACCPFHHEKTPSFTVSQKKQFYHCFGCGAHGNAISFLMEYDKL
EFVEAIEELAGMLGLEIPRENKPHFHGKQINLQTKRNLYELMQEIAQFYQQQLAQHIPAQSYLQQRGLSPEVISRFQIGF
VPNSFDAVLQRFGQQKEDQQKLFDLGMLSRSEQGKIYDRFRHRIMFPIRDRRGKTIAFGGRVLGDEKPKYLNSPESATYH
KGNELYGLYEALQANESPEMLLVVEGYMDVVALAQFGVDYAVASLGTATTAEQIQLLFRSTEQAICCYDGDRAGREAAWR
AFENALPYLEDGRQLKFVFLPDGEDPDSFIRQHGKASFEQYMQKALSLSEFLFTSLAPQVDFSSKEGKTKLAALAVPLIK
KIPGDMLRLSLRNTLAQKLGILDQAQLESLIPSYSEMKITASPQPVKRTPMRVLIGLLLQNPELAQLVPDLSPLRTLNEP
GLDLLEKLTALCQEKVGITTGQILEYWRDTEHSKALEILASWNHLVEDTQIEETFKSTLRYLYFQLIEHEIDLLIAKDRS
EGLNMNERKKLTQLLVKKQQKA
>Mature_582_residues
MKGSIPRTFIDDLLAKTDIVELVNSRVKLKKAGRDYQACCPFHHEKTPSFTVSQKKQFYHCFGCGAHGNAISFLMEYDKL
EFVEAIEELAGMLGLEIPRENKPHFHGKQINLQTKRNLYELMQEIAQFYQQQLAQHIPAQSYLQQRGLSPEVISRFQIGF
VPNSFDAVLQRFGQQKEDQQKLFDLGMLSRSEQGKIYDRFRHRIMFPIRDRRGKTIAFGGRVLGDEKPKYLNSPESATYH
KGNELYGLYEALQANESPEMLLVVEGYMDVVALAQFGVDYAVASLGTATTAEQIQLLFRSTEQAICCYDGDRAGREAAWR
AFENALPYLEDGRQLKFVFLPDGEDPDSFIRQHGKASFEQYMQKALSLSEFLFTSLAPQVDFSSKEGKTKLAALAVPLIK
KIPGDMLRLSLRNTLAQKLGILDQAQLESLIPSYSEMKITASPQPVKRTPMRVLIGLLLQNPELAQLVPDLSPLRTLNEP
GLDLLEKLTALCQEKVGITTGQILEYWRDTEHSKALEILASWNHLVEDTQIEETFKSTLRYLYFQLIEHEIDLLIAKDRS
EGLNMNERKKLTQLLVKKQQKA

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain

Homologues:

Organism=Escherichia coli, GI1789447, Length=576, Percent_Identity=57.2916666666667, Blast_Score=695, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRIM_PASMU (Q9CLI9)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246177.1
- ProteinModelPortal:   Q9CLI9
- GeneID:   1244587
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1240
- NMPDR:   fig|272843.1.peg.1240
- HOGENOM:   HBG573408
- OMA:   GDALPKY
- ProtClustDB:   PRK05667
- BioCyc:   PMUL272843:PM1240-MONOMER
- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694
- Gene3D:   G3DSA:1.10.860.10
- Gene3D:   G3DSA:3.90.980.10
- Gene3D:   G3DSA:3.90.580.10
- SMART:   SM00766
- SMART:   SM00493
- SMART:   SM00400
- TIGRFAMs:   TIGR01391

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2

EC number: 2.7.7.-

Molecular weight: Translated: 66309; Mature: 66309

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGSIPRTFIDDLLAKTDIVELVNSRVKLKKAGRDYQACCPFHHEKTPSFTVSQKKQFYH
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCHHHHHHHHH
CFGCGAHGNAISFLMEYDKLEFVEAIEELAGMLGLEIPRENKPHFHGKQINLQTKRNLYE
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECHHHHHHHHH
LMQEIAQFYQQQLAQHIPAQSYLQQRGLSPEVISRFQIGFVPNSFDAVLQRFGQQKEDQQ
HHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHH
KLFDLGMLSRSEQGKIYDRFRHRIMFPIRDRRGKTIAFGGRVLGDEKPKYLNSPESATYH
HHHHHHHHCCCCCCHHHHHHHHHEECCCCCCCCCEEEECCEECCCCCCCCCCCCCCCCCC
KGNELYGLYEALQANESPEMLLVVEGYMDVVALAQFGVDYAVASLGTATTAEQIQLLFRS
CCCHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
TEQAICCYDGDRAGREAAWRAFENALPYLEDGRQLKFVFLPDGEDPDSFIRQHGKASFEQ
CCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCHHHHH
YMQKALSLSEFLFTSLAPQVDFSSKEGKTKLAALAVPLIKKIPGDMLRLSLRNTLAQKLG
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHC
ILDQAQLESLIPSYSEMKITASPQPVKRTPMRVLIGLLLQNPELAQLVPDLSPLRTLNEP
CCCHHHHHHHCCCCCCEEEECCCCCCHHCHHHHHHHHHHCCCHHHHHCCCCHHHHHCCCC
GLDLLEKLTALCQEKVGITTGQILEYWRDTEHSKALEILASWNHLVEDTQIEETFKSTLR
CHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLYFQLIEHEIDLLIAKDRSEGLNMNERKKLTQLLVKKQQKA
HHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKGSIPRTFIDDLLAKTDIVELVNSRVKLKKAGRDYQACCPFHHEKTPSFTVSQKKQFYH
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCHHHHHHHHH
CFGCGAHGNAISFLMEYDKLEFVEAIEELAGMLGLEIPRENKPHFHGKQINLQTKRNLYE
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECHHHHHHHHH
LMQEIAQFYQQQLAQHIPAQSYLQQRGLSPEVISRFQIGFVPNSFDAVLQRFGQQKEDQQ
HHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHH
KLFDLGMLSRSEQGKIYDRFRHRIMFPIRDRRGKTIAFGGRVLGDEKPKYLNSPESATYH
HHHHHHHHCCCCCCHHHHHHHHHEECCCCCCCCCEEEECCEECCCCCCCCCCCCCCCCCC
KGNELYGLYEALQANESPEMLLVVEGYMDVVALAQFGVDYAVASLGTATTAEQIQLLFRS
CCCHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
TEQAICCYDGDRAGREAAWRAFENALPYLEDGRQLKFVFLPDGEDPDSFIRQHGKASFEQ
CCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCHHHHH
YMQKALSLSEFLFTSLAPQVDFSSKEGKTKLAALAVPLIKKIPGDMLRLSLRNTLAQKLG
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHC
ILDQAQLESLIPSYSEMKITASPQPVKRTPMRVLIGLLLQNPELAQLVPDLSPLRTLNEP
CCCHHHHHHHCCCCCCEEEECCCCCCHHCHHHHHHHHHHCCCHHHHHCCCCHHHHHCCCC
GLDLLEKLTALCQEKVGITTGQILEYWRDTEHSKALEILASWNHLVEDTQIEETFKSTLR
CHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLYFQLIEHEIDLLIAKDRSEGLNMNERKKLTQLLVKKQQKA
HHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100