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The map label for this gene is hisB

Identifier: 15603065

GI number: 15603065

Start: 1394514

End: 1395605

Strand: Direct

Name: hisB

Synonym: PM1200

Alternate gene names: 15603065

Gene position: 1394514-1395605 (Clockwise)

Preceding gene: 15603064

Following gene: 15603066

Centisome position: 61.77

GC content: 43.04

Gene sequence:

>1092_bases
ATGACACAACAATCTATTCTTTTTATCGACCGTGACGGGACTTTAATTGATGAACCCAAAACCGATTTTCAAATTGACAG
TTTGGAAAAATTACAATTTGAGCCGAATGTTATCCCCGCGTTATTAAAGCTCAAACCCTATTACCGTTTCGTAATGGTCA
GTAACCAAGACGGGCTAGGTACGGCATCTTTCCCACAGGAAAATTTTGATAAACCGCACAATGCGATGATGGCGTTATTT
AAATCACAAGGTATCGAGTTTGATGAGGTGTTAATTTGCCCTCATAAACCTGAAGACGAGTGCGATTGTCGTAAGCCGAA
AATTAAATTATTGAAAAAATACATTGATAAAAAATTGTTTGACCCTACCACCAGCTTTGTAATTGGCGATCGTGCCACTG
ACGTGCAACTGGCAGAAAATCTCGGTATTCGAGCCTTGCAATATGATCGTGAAAAGCTCAATTGGGATCTGATTGTGGAA
AAATTGTTGCCAAAACAGACCGCACTTTTAGACCGTAAACCACGCTATGCGGAAGTGGTTCGCACCACCAAAGAAACGGA
CATTAAAGTGCAAGTGTGGTTGGACGAAACAGGGATGAACGACATCAAAACGGGTGTGGGCTTTTTCGACCATATGCTTG
ATCAAATCGCCACCCACGGTGGCTTTAGAATGAATGTGTACTGCAAAGGCGATTTGTGGATTGACGAACATCACACCGTC
GAAGACACTGCCCTCGCCCTCGGTACCGCATTAAAACAAGCCCTTGGCGATAAACGAGGCATTCAACGCTTTGGCTTTGT
GTTACCAATGGACGAATGCAAGGCAGAATGTACGATGGATCTGTCTGGTCGCCCGTATTTCAAATTCAAAGCCAAATTTA
AACGCGATAAAGTGGGTGATTTCAGCACCGAAATGACCGAGCATTTTTTCCAATCTCTCGCTTATACCTTAATGGCAACC
TTACATCTCAAAACCAAAGGCGATAACGACCACCACAAAATCGAAAGCCTGTTTAAAGTGTTTGGGCGTACGTTAAGACA
GTGTATTAAGGTTGAAGGGAATGAGTTGCCGAGTAGTAAGGGGGTTTTGTGA

Upstream 100 bases:

>100_bases
CACGGCAGAGGAAAATCGTCGTGTCATTGAAGCAATCCGTAAAATTGCCTAAGATACAAACGAAAAACCAACAGATTAAA
ATGAAAAACAGGATAACTCT

Downstream 100 bases:

>100_bases
GTAAAAAGGATGGGTATTTAGAGTTTTTTTCTTATTTTAATATTAGTGAAGAAAAATTTATTAATTTTGCTAAGGAAAGC
ATTATTTTTATCAAACAAGA

Product: imidazole glycerol-phosphate dehydratase/histidinol phosphatase

Products: NA

Alternate protein names: Histidinol-phosphatase; Imidazoleglycerol-phosphate dehydratase; IGPD

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MTQQSILFIDRDGTLIDEPKTDFQIDSLEKLQFEPNVIPALLKLKPYYRFVMVSNQDGLGTASFPQENFDKPHNAMMALF
KSQGIEFDEVLICPHKPEDECDCRKPKIKLLKKYIDKKLFDPTTSFVIGDRATDVQLAENLGIRALQYDREKLNWDLIVE
KLLPKQTALLDRKPRYAEVVRTTKETDIKVQVWLDETGMNDIKTGVGFFDHMLDQIATHGGFRMNVYCKGDLWIDEHHTV
EDTALALGTALKQALGDKRGIQRFGFVLPMDECKAECTMDLSGRPYFKFKAKFKRDKVGDFSTEMTEHFFQSLAYTLMAT
LHLKTKGDNDHHKIESLFKVFGRTLRQCIKVEGNELPSSKGVL

Sequences:

>Translated_363_residues
MTQQSILFIDRDGTLIDEPKTDFQIDSLEKLQFEPNVIPALLKLKPYYRFVMVSNQDGLGTASFPQENFDKPHNAMMALF
KSQGIEFDEVLICPHKPEDECDCRKPKIKLLKKYIDKKLFDPTTSFVIGDRATDVQLAENLGIRALQYDREKLNWDLIVE
KLLPKQTALLDRKPRYAEVVRTTKETDIKVQVWLDETGMNDIKTGVGFFDHMLDQIATHGGFRMNVYCKGDLWIDEHHTV
EDTALALGTALKQALGDKRGIQRFGFVLPMDECKAECTMDLSGRPYFKFKAKFKRDKVGDFSTEMTEHFFQSLAYTLMAT
LHLKTKGDNDHHKIESLFKVFGRTLRQCIKVEGNELPSSKGVL
>Mature_362_residues
TQQSILFIDRDGTLIDEPKTDFQIDSLEKLQFEPNVIPALLKLKPYYRFVMVSNQDGLGTASFPQENFDKPHNAMMALFK
SQGIEFDEVLICPHKPEDECDCRKPKIKLLKKYIDKKLFDPTTSFVIGDRATDVQLAENLGIRALQYDREKLNWDLIVEK
LLPKQTALLDRKPRYAEVVRTTKETDIKVQVWLDETGMNDIKTGVGFFDHMLDQIATHGGFRMNVYCKGDLWIDEHHTVE
DTALALGTALKQALGDKRGIQRFGFVLPMDECKAECTMDLSGRPYFKFKAKFKRDKVGDFSTEMTEHFFQSLAYTLMATL
HLKTKGDNDHHKIESLFKVFGRTLRQCIKVEGNELPSSKGVL

Specific function: Histidine biosynthesis; sixth step. Histidine biosynthesis; eighth step. [C]

COG id: COG0131

COG function: function code E; Imidazoleglycerol-phosphate dehydratase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the imidazoleglycerol-phosphate dehydratase family

Homologues:

Organism=Escherichia coli, GI87082027, Length=361, Percent_Identity=68.1440443213296, Blast_Score=520, Evalue=1e-149,
Organism=Escherichia coli, GI1786399, Length=150, Percent_Identity=30.6666666666667, Blast_Score=72, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324776, Length=166, Percent_Identity=46.3855421686747, Blast_Score=157, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS7_PASMU (P57920)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246137.1
- ProteinModelPortal:   P57920
- GeneID:   1244547
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1200
- NMPDR:   fig|272843.1.peg.1200
- HOGENOM:   HBG289010
- OMA:   QDGLGTP
- ProtClustDB:   PRK05446
- BioCyc:   PMUL272843:PM1200-MONOMER
- BRENDA:   3.1.3.15
- BRENDA:   4.2.1.19
- GO:   GO:0005737
- HAMAP:   MF_01022
- InterPro:   IPR023214
- InterPro:   IPR006549
- InterPro:   IPR020566
- InterPro:   IPR005954
- InterPro:   IPR006543
- InterPro:   IPR000807
- InterPro:   IPR020565
- InterPro:   IPR020568
- Gene3D:   G3DSA:3.40.50.1000
- TIGRFAMs:   TIGR01662
- TIGRFAMs:   TIGR01261
- TIGRFAMs:   TIGR01656

Pfam domain/function: PF00475 IGPD; SSF54211 Ribosomal_S5_D2-typ_fold; SSF56784 SSF56784

EC number: =3.1.3.15; =4.2.1.19

Molecular weight: Translated: 41671; Mature: 41539

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00954 IGP_DEHYDRATASE_1; PS00955 IGP_DEHYDRATASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQQSILFIDRDGTLIDEPKTDFQIDSLEKLQFEPNVIPALLKLKPYYRFVMVSNQDGLG
CCCCEEEEEECCCCEECCCCCCCCCCCHHHHCCCCCHHHHHHHCCCEEEEEEEECCCCCC
TASFPQENFDKPHNAMMALFKSQGIEFDEVLICPHKPEDECDCRKPKIKLLKKYIDKKLF
CCCCCHHHCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHC
DPTTSFVIGDRATDVQLAENLGIRALQYDREKLNWDLIVEKLLPKQTALLDRKPRYAEVV
CCCCCEEECCCCCCHHHHHCCCCEEEEHHHHHCCHHHHHHHHCCHHHHHHCCCCHHHHHH
RTTKETDIKVQVWLDETGMNDIKTGVGFFDHMLDQIATHGGFRMNVYCKGDLWIDEHHTV
HHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEECCCCCH
EDTALALGTALKQALGDKRGIQRFGFVLPMDECKAECTMDLSGRPYFKFKAKFKRDKVGD
HHHHHHHHHHHHHHHCHHHHHHHHCCCCCHHHHCCEEEEECCCCCEEEEEECCCCCCCCC
FSTEMTEHFFQSLAYTLMATLHLKTKGDNDHHKIESLFKVFGRTLRQCIKVEGNELPSSK
HHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GVL
CCC
>Mature Secondary Structure 
TQQSILFIDRDGTLIDEPKTDFQIDSLEKLQFEPNVIPALLKLKPYYRFVMVSNQDGLG
CCCEEEEEECCCCEECCCCCCCCCCCHHHHCCCCCHHHHHHHCCCEEEEEEEECCCCCC
TASFPQENFDKPHNAMMALFKSQGIEFDEVLICPHKPEDECDCRKPKIKLLKKYIDKKLF
CCCCCHHHCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHC
DPTTSFVIGDRATDVQLAENLGIRALQYDREKLNWDLIVEKLLPKQTALLDRKPRYAEVV
CCCCCEEECCCCCCHHHHHCCCCEEEEHHHHHCCHHHHHHHHCCHHHHHHCCCCHHHHHH
RTTKETDIKVQVWLDETGMNDIKTGVGFFDHMLDQIATHGGFRMNVYCKGDLWIDEHHTV
HHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEECCCCCH
EDTALALGTALKQALGDKRGIQRFGFVLPMDECKAECTMDLSGRPYFKFKAKFKRDKVGD
HHHHHHHHHHHHHHHCHHHHHHHHCCCCCHHHHCCEEEEECCCCCEEEEEECCCCCCCCC
FSTEMTEHFFQSLAYTLMATLHLKTKGDNDHHKIESLFKVFGRTLRQCIKVEGNELPSSK
HHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GVL
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100