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The map label for this gene is plsB

Identifier: 15603047

GI number: 15603047

Start: 1375875

End: 1378304

Strand: Direct

Name: plsB

Synonym: PM1182

Alternate gene names: 15603047

Gene position: 1375875-1378304 (Clockwise)

Preceding gene: 15603041

Following gene: 15603049

Centisome position: 60.95

GC content: 40.21

Gene sequence:

>2430_bases
ATGTTTAGTTTGCTAAATATGTACCGCAAAGTATTGGAATTACCTTTATCCTTTTTAGTCAAAAACAACCCGATTCCACA
TCATCCTATCGAAGAATTAAAATTAGACCCAACTCAACCGATGGTGTATGTGCTCCCTTATACTTCACAAACAGACTTAG
TGATTTTTCGTAAAAACTGTTTAAGTGTCGGTTTGCCTGATCCTTTAGAAAAAAATGAAATTGAAGGAAAATCCCTTCCT
CGCTTTGTCTTCTTAGATGAAGGTCGCCGTTTTTTTAAATCAAAAGGGGCAAAAGAAGAAACCATTCAAGTGTTTAATCA
ATATCTTGAATTACATCACCAACGTTCTGACTTAGATGTCCAAATTATTCCAGTTTCAGTACTTTGGGGACGTTCACCTG
GTCACGAAAAACAATCAGGCTTACCTCAATTACGTTTATTAAGTGGTCTGCAAAAATTTATCGCCGCCGTGTGGTTTGGA
CGCGATACCTTCGTTCGTTTTTCACAAGCCGTTTCCTTACGTTACATGGTGAATGAACATGGTTCAGACCAAGCCATTTC
ACATAAATTAGCGCGTGTGGCGAAAATTCACTTTTCTAAACAGCGCATTTCTGCAACAGGTCCTCGCCTACCAAATCGTG
AAGCGATGTTTAACAAATTATTGGCCTCACCAACAATTTTAGATGCGATTCAAGACGAAGCCAAAGCAAAGAACATTAGC
GAAGAAAAGGCAACTCAAGAAGCGAAAAAGATTCTTGATGAAATCGCTGCCAATGTGAATTATGAAGGCTTACGCGTCGC
AGACCGTTTCTTACGTTGGTTATGGAATAAACTCTATCAAGGTATTGATGTACAAAATGCAGATCGTGTACGCAAACTGG
CATTAGACGGTCATGAAATTGTTTACGTACCTTGTCATCGTAGCCATATTGACTATTTATTACTGTCCTATGTGTTATAC
CACCAAGGTTTAGTGCCACCACATATTGCCGCTGGGATTAACTTAAATTTCTGGCCAGTGGGCGGTATGTTCCGTCGCGG
TGGCGCCTTCTTTATTCGCCGTACATTCAAAGGTAACCGCTTATATTCCACCATTTTCCGTGAATATTTAGCCGAATTAT
TCCATCGTGGCTATTCAGTGGAATACTTCATTGAGGGGGGACGTTCGCGTACGGGACGTTTACTCGCACCCAAAACAGGC
ATGATGTCGATGACCCTACAAGCCCTGTTACAAGGGCAAACCCGTCCGATTTCAGTGGTTCCCGTGTATGTCGGTTATGA
ACACGTCCTAGAAGTGGACACCTACGCTAAAGAATTACGTGGTGCAGCGAAAGAAAAAGAAAATGCCGGACTGGTTTTAC
GCGTGATTAAGAAACTCCGTAATTTGGGTAAAGGTTATGTGAACTTTGGCGAACCTATCACCTTAAGTAACTACCTCAAT
CAACACTATCCCGAGTGGAAAGAAAATACCCAAGCAGAGGAGCGTGCGAAGTGGTTTAATCAAGCCGTTGATAGCATATC
TCATCAAGTGATGGTGAATATTAATAAAGCGGCTGCTGTCAATGCGATGAACTTGACAGGTACCGCGTTACTTTCTTCAC
GTCAACGTGCTCTTTCGCGTGAACAATTGCTTGAACAGTTAGAAAGTTATCAACAATTCTTACAAAATGTGCCTTATTCA
CAAGATATTGTGGTGCCAACGGAATCACCAGAAAAAATGTTAGAACACATTTTGCAATTAGAACGAGTTGGCATTATTGT
TGAAAAAGATAACTTTGGTGAAATTGTTCGCTTAGAACGAAATTCTGCGGTGTTAATGACCTATTATCGAAATAACATTC
AGCATCTGTTTGTACTACCGTCATTGGTCGCCAGTATTGTGTTGCATTATGAAGCCATTCAAAAACCACTGGTACTCGAA
GCGGTAAGTAAGATTTATCCGTTCTTAAAAAGTGAACTTTTCCTGTCTTTCTCGTATGAAGAATTACAAGATCGAGTGAA
TCACATTATTACTGAATTTGAACGTCAAGAAGTGATTAAATGTAACGCGAATATGTTATCAATTAATAAACCGCGTGTTC
GTATGTTACAACTCTGGTCATCCGGTGTCAGAGAGATTTTACAGCGCTATTACATCACCGTCAGTATTTTAGAAAAAGAT
CCTGCCACACCACGTGCAAGCCTAGAAAAAGATAGTCAATCTGTGGCGCAACGTCTATCTGTGTTACATGGCATCAACGC
GCCGGAGTTTTTTGATAAAGCCGTATTTTCTGCCTTTATCGCGAGCTTAAAACAAGAAGGCTATTTCAATGAGGAAGGTA
TTGCCAATACAGAAAAACTCAATGAAATCACAGAGATCTTAAATCAAGTGATCTCCACTGAAGTTTGCTTAACGATTAAA
GGGGCAATTGCCAAAATTGAAGAAGAATAA

Upstream 100 bases:

>100_bases
AGACGATGCGCTTCTTTTAGACAACGACTAACGATTTTTCATTTTTATGGTAAACTAACGTAAATTTTTCTAATAAACCT
ATAAAGAACGGAACTTCATT

Downstream 100 bases:

>100_bases
TCTGTGATACCTATCTGATAAAAAATAAGTGCGGTCAAAAAATCTAAATTTTGACCGCACTTTAGTATTCCTCTCATTGC
CCATGAAACCGCGGGTTTCT

Product: glycerol-3-phosphate acyltransferase

Products: NA

Alternate protein names: GPAT

Number of amino acids: Translated: 809; Mature: 809

Protein sequence:

>809_residues
MFSLLNMYRKVLELPLSFLVKNNPIPHHPIEELKLDPTQPMVYVLPYTSQTDLVIFRKNCLSVGLPDPLEKNEIEGKSLP
RFVFLDEGRRFFKSKGAKEETIQVFNQYLELHHQRSDLDVQIIPVSVLWGRSPGHEKQSGLPQLRLLSGLQKFIAAVWFG
RDTFVRFSQAVSLRYMVNEHGSDQAISHKLARVAKIHFSKQRISATGPRLPNREAMFNKLLASPTILDAIQDEAKAKNIS
EEKATQEAKKILDEIAANVNYEGLRVADRFLRWLWNKLYQGIDVQNADRVRKLALDGHEIVYVPCHRSHIDYLLLSYVLY
HQGLVPPHIAAGINLNFWPVGGMFRRGGAFFIRRTFKGNRLYSTIFREYLAELFHRGYSVEYFIEGGRSRTGRLLAPKTG
MMSMTLQALLQGQTRPISVVPVYVGYEHVLEVDTYAKELRGAAKEKENAGLVLRVIKKLRNLGKGYVNFGEPITLSNYLN
QHYPEWKENTQAEERAKWFNQAVDSISHQVMVNINKAAAVNAMNLTGTALLSSRQRALSREQLLEQLESYQQFLQNVPYS
QDIVVPTESPEKMLEHILQLERVGIIVEKDNFGEIVRLERNSAVLMTYYRNNIQHLFVLPSLVASIVLHYEAIQKPLVLE
AVSKIYPFLKSELFLSFSYEELQDRVNHIITEFERQEVIKCNANMLSINKPRVRMLQLWSSGVREILQRYYITVSILEKD
PATPRASLEKDSQSVAQRLSVLHGINAPEFFDKAVFSAFIASLKQEGYFNEEGIANTEKLNEITEILNQVISTEVCLTIK
GAIAKIEEE

Sequences:

>Translated_809_residues
MFSLLNMYRKVLELPLSFLVKNNPIPHHPIEELKLDPTQPMVYVLPYTSQTDLVIFRKNCLSVGLPDPLEKNEIEGKSLP
RFVFLDEGRRFFKSKGAKEETIQVFNQYLELHHQRSDLDVQIIPVSVLWGRSPGHEKQSGLPQLRLLSGLQKFIAAVWFG
RDTFVRFSQAVSLRYMVNEHGSDQAISHKLARVAKIHFSKQRISATGPRLPNREAMFNKLLASPTILDAIQDEAKAKNIS
EEKATQEAKKILDEIAANVNYEGLRVADRFLRWLWNKLYQGIDVQNADRVRKLALDGHEIVYVPCHRSHIDYLLLSYVLY
HQGLVPPHIAAGINLNFWPVGGMFRRGGAFFIRRTFKGNRLYSTIFREYLAELFHRGYSVEYFIEGGRSRTGRLLAPKTG
MMSMTLQALLQGQTRPISVVPVYVGYEHVLEVDTYAKELRGAAKEKENAGLVLRVIKKLRNLGKGYVNFGEPITLSNYLN
QHYPEWKENTQAEERAKWFNQAVDSISHQVMVNINKAAAVNAMNLTGTALLSSRQRALSREQLLEQLESYQQFLQNVPYS
QDIVVPTESPEKMLEHILQLERVGIIVEKDNFGEIVRLERNSAVLMTYYRNNIQHLFVLPSLVASIVLHYEAIQKPLVLE
AVSKIYPFLKSELFLSFSYEELQDRVNHIITEFERQEVIKCNANMLSINKPRVRMLQLWSSGVREILQRYYITVSILEKD
PATPRASLEKDSQSVAQRLSVLHGINAPEFFDKAVFSAFIASLKQEGYFNEEGIANTEKLNEITEILNQVISTEVCLTIK
GAIAKIEEE
>Mature_809_residues
MFSLLNMYRKVLELPLSFLVKNNPIPHHPIEELKLDPTQPMVYVLPYTSQTDLVIFRKNCLSVGLPDPLEKNEIEGKSLP
RFVFLDEGRRFFKSKGAKEETIQVFNQYLELHHQRSDLDVQIIPVSVLWGRSPGHEKQSGLPQLRLLSGLQKFIAAVWFG
RDTFVRFSQAVSLRYMVNEHGSDQAISHKLARVAKIHFSKQRISATGPRLPNREAMFNKLLASPTILDAIQDEAKAKNIS
EEKATQEAKKILDEIAANVNYEGLRVADRFLRWLWNKLYQGIDVQNADRVRKLALDGHEIVYVPCHRSHIDYLLLSYVLY
HQGLVPPHIAAGINLNFWPVGGMFRRGGAFFIRRTFKGNRLYSTIFREYLAELFHRGYSVEYFIEGGRSRTGRLLAPKTG
MMSMTLQALLQGQTRPISVVPVYVGYEHVLEVDTYAKELRGAAKEKENAGLVLRVIKKLRNLGKGYVNFGEPITLSNYLN
QHYPEWKENTQAEERAKWFNQAVDSISHQVMVNINKAAAVNAMNLTGTALLSSRQRALSREQLLEQLESYQQFLQNVPYS
QDIVVPTESPEKMLEHILQLERVGIIVEKDNFGEIVRLERNSAVLMTYYRNNIQHLFVLPSLVASIVLHYEAIQKPLVLE
AVSKIYPFLKSELFLSFSYEELQDRVNHIITEFERQEVIKCNANMLSINKPRVRMLQLWSSGVREILQRYYITVSILEKD
PATPRASLEKDSQSVAQRLSVLHGINAPEFFDKAVFSAFIASLKQEGYFNEEGIANTEKLNEITEILNQVISTEVCLTIK
GAIAKIEEE

Specific function: De novo phospholipid biosynthesis; first step. [C]

COG id: COG2937

COG function: function code I; Glycerol-3-phosphate O-acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GPAT/DAPAT family

Homologues:

Organism=Homo sapiens, GI7657134, Length=403, Percent_Identity=29.7766749379653, Blast_Score=167, Evalue=3e-41,
Organism=Homo sapiens, GI190358539, Length=421, Percent_Identity=23.5154394299287, Blast_Score=124, Evalue=4e-28,
Organism=Escherichia coli, GI87082362, Length=798, Percent_Identity=57.7694235588972, Blast_Score=953, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25147672, Length=346, Percent_Identity=29.7687861271676, Blast_Score=159, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI71988723, Length=522, Percent_Identity=24.1379310344828, Blast_Score=132, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI71988728, Length=519, Percent_Identity=24.0847784200385, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI17864692, Length=277, Percent_Identity=33.5740072202166, Blast_Score=159, Evalue=5e-39,
Organism=Drosophila melanogaster, GI24650754, Length=490, Percent_Identity=27.7551020408163, Blast_Score=145, Evalue=9e-35,
Organism=Drosophila melanogaster, GI21357731, Length=490, Percent_Identity=27.9591836734694, Blast_Score=145, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24650752, Length=490, Percent_Identity=27.7551020408163, Blast_Score=145, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PLSB_PASMU (Q9CLN7)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246119.1
- ProteinModelPortal:   Q9CLN7
- GeneID:   1244529
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1182
- NMPDR:   fig|272843.1.peg.1182
- HOGENOM:   HBG296590
- OMA:   VIYVPCH
- ProtClustDB:   PRK04974
- BioCyc:   PMUL272843:PM1182-MONOMER
- BRENDA:   2.3.1.15
- HAMAP:   MF_00393
- InterPro:   IPR002123
- InterPro:   IPR022284
- SMART:   SM00563
- TIGRFAMs:   TIGR03703

Pfam domain/function: PF01553 Acyltransferase

EC number: =2.3.1.15

Molecular weight: Translated: 92675; Mature: 92675

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSLLNMYRKVLELPLSFLVKNNPIPHHPIEELKLDPTQPMVYVLPYTSQTDLVIFRKNC
CCHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHCCCCCCCCEEEEEECCCCCCEEEEECCH
LSVGLPDPLEKNEIEGKSLPRFVFLDEGRRFFKSKGAKEETIQVFNQYLELHHQRSDLDV
HCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCE
QIIPVSVLWGRSPGHEKQSGLPQLRLLSGLQKFIAAVWFGRDTFVRFSQAVSLRYMVNEH
EEEEEEEEECCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
GSDQAISHKLARVAKIHFSKQRISATGPRLPNREAMFNKLLASPTILDAIQDEAKAKNIS
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCC
EEKATQEAKKILDEIAANVNYEGLRVADRFLRWLWNKLYQGIDVQNADRVRKLALDGHEI
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEE
VYVPCHRSHIDYLLLSYVLYHQGLVPPHIAAGINLNFWPVGGMFRRGGAFFIRRTFKGNR
EEECCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCCEEECCCCHHHCCCEEEEEEECCCCH
LYSTIFREYLAELFHRGYSVEYFIEGGRSRTGRLLAPKTGMMSMTLQALLQGQTRPISVV
HHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEECCCCCHHHHHHHHHHCCCCCCEEEE
PVYVGYEHVLEVDTYAKELRGAAKEKENAGLVLRVIKKLRNLGKGYVNFGEPITLSNYLN
EEEECHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEHHHHHH
QHYPEWKENTQAEERAKWFNQAVDSISHQVMVNINKAAAVNAMNLTGTALLSSRQRALSR
HCCCCHHCCCCHHHHHHHHHHHHHHCCCEEEEEECHHHEEECCCCHHHHHHHHHHHHHHH
EQLLEQLESYQQFLQNVPYSQDIVVPTESPEKMLEHILQLERVGIIVEKDNFGEIVRLER
HHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHCEEEEECCCCCEEEEEC
NSAVLMTYYRNNIQHLFVLPSLVASIVLHYEAIQKPLVLEAVSKIYPFLKSELFLSFSYE
CCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHH
ELQDRVNHIITEFERQEVIKCNANMLSINKPRVRMLQLWSSGVREILQRYYITVSILEKD
HHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHEEEEEEECC
PATPRASLEKDSQSVAQRLSVLHGINAPEFFDKAVFSAFIASLKQEGYFNEEGIANTEKL
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
NEITEILNQVISTEVCLTIKGAIAKIEEE
HHHHHHHHHHHCHHHHEEEHHHHHHCCCC
>Mature Secondary Structure
MFSLLNMYRKVLELPLSFLVKNNPIPHHPIEELKLDPTQPMVYVLPYTSQTDLVIFRKNC
CCHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHCCCCCCCCEEEEEECCCCCCEEEEECCH
LSVGLPDPLEKNEIEGKSLPRFVFLDEGRRFFKSKGAKEETIQVFNQYLELHHQRSDLDV
HCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCE
QIIPVSVLWGRSPGHEKQSGLPQLRLLSGLQKFIAAVWFGRDTFVRFSQAVSLRYMVNEH
EEEEEEEEECCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
GSDQAISHKLARVAKIHFSKQRISATGPRLPNREAMFNKLLASPTILDAIQDEAKAKNIS
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCC
EEKATQEAKKILDEIAANVNYEGLRVADRFLRWLWNKLYQGIDVQNADRVRKLALDGHEI
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEE
VYVPCHRSHIDYLLLSYVLYHQGLVPPHIAAGINLNFWPVGGMFRRGGAFFIRRTFKGNR
EEECCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCCEEECCCCHHHCCCEEEEEEECCCCH
LYSTIFREYLAELFHRGYSVEYFIEGGRSRTGRLLAPKTGMMSMTLQALLQGQTRPISVV
HHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEECCCCCHHHHHHHHHHCCCCCCEEEE
PVYVGYEHVLEVDTYAKELRGAAKEKENAGLVLRVIKKLRNLGKGYVNFGEPITLSNYLN
EEEECHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEHHHHHH
QHYPEWKENTQAEERAKWFNQAVDSISHQVMVNINKAAAVNAMNLTGTALLSSRQRALSR
HCCCCHHCCCCHHHHHHHHHHHHHHCCCEEEEEECHHHEEECCCCHHHHHHHHHHHHHHH
EQLLEQLESYQQFLQNVPYSQDIVVPTESPEKMLEHILQLERVGIIVEKDNFGEIVRLER
HHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHCEEEEECCCCCEEEEEC
NSAVLMTYYRNNIQHLFVLPSLVASIVLHYEAIQKPLVLEAVSKIYPFLKSELFLSFSYE
CCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHH
ELQDRVNHIITEFERQEVIKCNANMLSINKPRVRMLQLWSSGVREILQRYYITVSILEKD
HHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHEEEEEEECC
PATPRASLEKDSQSVAQRLSVLHGINAPEFFDKAVFSAFIASLKQEGYFNEEGIANTEKL
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
NEITEILNQVISTEVCLTIKGAIAKIEEE
HHHHHHHHHHHCHHHHEEEHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11248100