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The map label for this gene is lexA

Identifier: 15603046

GI number: 15603046

Start: 1374781

End: 1375416

Strand: Reverse

Name: lexA

Synonym: PM1181

Alternate gene names: 15603046

Gene position: 1375416-1374781 (Counterclockwise)

Preceding gene: 15603048

Following gene: 15603045

Centisome position: 60.93

GC content: 41.35

Gene sequence:

>636_bases
ATGAAGCCATTTAAAGCATTGACCGCGCGTCAACAAGAAGTCTATGACTTGTTAAAACGTCATTTAGAAAATACAGGTAT
GCCACCAACAAGGGCAGAAATTTCAAAAGAATTAGGTTTTCGCTCACCGAATGCGGCAGAAGAACATTTAAAAGCTTTAG
CGCGTAAAGGCGTGATTGAGATTATTTCCGGGACATCCCGTGGTATTCGTTTATTGCTTGAAGAGAGCGAGAGTGATGAA
AACCAAGGCTTACCTTTAGTCGGGCGTGTTGCCGCAGGTGAGCCGATTTTAGCGGAACAACATATTGAAGGAACGTATCA
AGTCGATGCCAATATGTTTAAACCGCAAGCCAACTTTTTGTTGAAAGTCTACGGACAATCGATGAAAAACATTGGTATTT
TAGATGGTGATCTGTTAGCAGTACATAGCACCAAAGATGTGCGTAATGGACAGATTGTGGTTGCACGCATTGAGGATGAA
GTGACAGTCAAGCGTTTGGAACGTAAAGGCTCGGTAATTTATCTGCATGCGGAAAATGAAGAGTTTGCGCCAATTGTGGT
TGATTTAAATCAGCAACCTAATTTTGAAATTGAAGGAATTGCTGTAGGGATTATCCGCAATAATGCTTGGATGTAA

Upstream 100 bases:

>100_bases
GTTATTTACAGGATAGGCTGACTATTACGAAGAAAAGATTGCAACGAACGTCTTTCTGTATATAATAACAGTTATTCTGT
ATAAAAAAACAGGGGTAAGC

Downstream 100 bases:

>100_bases
GCTCACTTTTTTCTGCTATTTTAAACTGACACCGCACTTTTCATCTTATTGAAAAGTGCGGTGATTTTTTACCAAATATT
GCCGATATTTGTTTGATTCA

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MKPFKALTARQQEVYDLLKRHLENTGMPPTRAEISKELGFRSPNAAEEHLKALARKGVIEIISGTSRGIRLLLEESESDE
NQGLPLVGRVAAGEPILAEQHIEGTYQVDANMFKPQANFLLKVYGQSMKNIGILDGDLLAVHSTKDVRNGQIVVARIEDE
VTVKRLERKGSVIYLHAENEEFAPIVVDLNQQPNFEIEGIAVGIIRNNAWM

Sequences:

>Translated_211_residues
MKPFKALTARQQEVYDLLKRHLENTGMPPTRAEISKELGFRSPNAAEEHLKALARKGVIEIISGTSRGIRLLLEESESDE
NQGLPLVGRVAAGEPILAEQHIEGTYQVDANMFKPQANFLLKVYGQSMKNIGILDGDLLAVHSTKDVRNGQIVVARIEDE
VTVKRLERKGSVIYLHAENEEFAPIVVDLNQQPNFEIEGIAVGIIRNNAWM
>Mature_211_residues
MKPFKALTARQQEVYDLLKRHLENTGMPPTRAEISKELGFRSPNAAEEHLKALARKGVIEIISGTSRGIRLLLEESESDE
NQGLPLVGRVAAGEPILAEQHIEGTYQVDANMFKPQANFLLKVYGQSMKNIGILDGDLLAVHSTKDVRNGQIVVARIEDE
VTVKRLERKGSVIYLHAENEEFAPIVVDLNQQPNFEIEGIAVGIIRNNAWM

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=208, Percent_Identity=72.5961538461538, Blast_Score=307, Evalue=3e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_PASMU (P57917)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246118.1
- ProteinModelPortal:   P57917
- SMR:   P57917
- MEROPS:   S24.001
- GeneID:   1244528
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1181
- NMPDR:   fig|272843.1.peg.1181
- HOGENOM:   HBG679610
- OMA:   IAENPDF
- ProtClustDB:   PRK00215
- BioCyc:   PMUL272843:PM1181-MONOMER
- BRENDA:   3.4.21.88
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 23506; Mature: 23506

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: ACT_SITE 127-127 ACT_SITE 164-164

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPFKALTARQQEVYDLLKRHLENTGMPPTRAEISKELGFRSPNAAEEHLKALARKGVIE
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCEE
IISGTSRGIRLLLEESESDENQGLPLVGRVAAGEPILAEQHIEGTYQVDANMFKPQANFL
EECCCCCCEEEEEECCCCCCCCCCCEEEEECCCCCCEEHHCCCCEEEECCCEECCHHHHH
LKVYGQSMKNIGILDGDLLAVHSTKDVRNGQIVVARIEDEVTVKRLERKGSVIYLHAENE
HHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEEEEEECCHHHHHHHHHCCCEEEEEECCC
EFAPIVVDLNQQPNFEIEGIAVGIIRNNAWM
CCCEEEEECCCCCCEEEEEEEEEEEECCCCC
>Mature Secondary Structure
MKPFKALTARQQEVYDLLKRHLENTGMPPTRAEISKELGFRSPNAAEEHLKALARKGVIE
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCEE
IISGTSRGIRLLLEESESDENQGLPLVGRVAAGEPILAEQHIEGTYQVDANMFKPQANFL
EECCCCCCEEEEEECCCCCCCCCCCEEEEECCCCCCEEHHCCCCEEEECCCEECCHHHHH
LKVYGQSMKNIGILDGDLLAVHSTKDVRNGQIVVARIEDEVTVKRLERKGSVIYLHAENE
HHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEEEEEECCHHHHHHHHHCCCEEEEEECCC
EFAPIVVDLNQQPNFEIEGIAVGIIRNNAWM
CCCEEEEECCCCCCEEEEEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100