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The map label for this gene is priB

Identifier: 15603044

GI number: 15603044

Start: 1373769

End: 1374095

Strand: Reverse

Name: priB

Synonym: PM1179

Alternate gene names: 15603044

Gene position: 1374095-1373769 (Counterclockwise)

Preceding gene: 15603045

Following gene: 15603043

Centisome position: 60.87

GC content: 38.53

Gene sequence:

>327_bases
ATGCTGAAGAGTAATTTAAATATTGATAATCGTTTTTCGTTGATAGGCATCGTCGCGAGTCAACCAAAACGACACCAAAG
TCCGAATGGCATTTATCATTGCCAATTTGTATTAGAACATCGTTCTATACAAGAAGAAGCCGGATTAAGCCGTCAGGCGT
GGTGTAAAATGCCAGTTCAAATGAGTGGCAATCAATTTAGAGAAAAAACTCAAAGCATTACGGTCGGCAGTCAGCTTTTG
GTGGTGGGATTTATTAGTTCTCACAAAACCGCAAATGGTTTAAGCCAATTAGTATTACATGCCGAGCAAATCGAATTTAT
AGATTAG

Upstream 100 bases:

>100_bases
ATCATTCGTACTAAGCACGCCGTAACAGAAGCGTCCCCAATGGTTAAAGCAAAAGACGATCGTAAAGCTTTAGCTGAAGT
TGAAAACAACGATTTTGAGG

Downstream 100 bases:

>100_bases
GAGACTAGCCAAATGGCACGTTATTTCCGTCGTCGTAAGTTCTGCCGTTTCACAGCGGAAAATGTAGTAGAAATCGATTA
TAAAGATGTGGCTACATTAA

Product: primosomal replication protein N

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 108; Mature: 108

Protein sequence:

>108_residues
MLKSNLNIDNRFSLIGIVASQPKRHQSPNGIYHCQFVLEHRSIQEEAGLSRQAWCKMPVQMSGNQFREKTQSITVGSQLL
VVGFISSHKTANGLSQLVLHAEQIEFID

Sequences:

>Translated_108_residues
MLKSNLNIDNRFSLIGIVASQPKRHQSPNGIYHCQFVLEHRSIQEEAGLSRQAWCKMPVQMSGNQFREKTQSITVGSQLL
VVGFISSHKTANGLSQLVLHAEQIEFID
>Mature_108_residues
MLKSNLNIDNRFSLIGIVASQPKRHQSPNGIYHCQFVLEHRSIQEEAGLSRQAWCKMPVQMSGNQFREKTQSITVGSQLL
VVGFISSHKTANGLSQLVLHAEQIEFID

Specific function: Binds single-stranded DNA at the primosome assembly site (PAS)

COG id: COG2965

COG function: function code L; Primosomal replication protein N

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SSB domain

Homologues:

Organism=Escherichia coli, GI1790645, Length=101, Percent_Identity=63.3663366336634, Blast_Score=132, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRIB_PASMU (Q9CLN9)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246116.1
- ProteinModelPortal:   Q9CLN9
- SMR:   Q9CLN9
- GeneID:   1244526
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1179
- NMPDR:   fig|272843.1.peg.1179
- HOGENOM:   HBG632331
- OMA:   WCRMPVI
- ProtClustDB:   PRK02801
- BioCyc:   PMUL272843:PM1179-MONOMER
- HAMAP:   MF_00720
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000424
- Gene3D:   G3DSA:2.40.50.140

Pfam domain/function: PF00436 SSB; SSF50249 Nucleic_acid_OB

EC number: NA

Molecular weight: Translated: 12148; Mature: 12148

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: PS50935 SSB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKSNLNIDNRFSLIGIVASQPKRHQSPNGIYHCQFVLEHRSIQEEAGLSRQAWCKMPVQ
CCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEEEEECHHHHHHHCCCCCHHHCCCCCC
MSGNQFREKTQSITVGSQLLVVGFISSHKTANGLSQLVLHAEQIEFID
CCCHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHEECC
>Mature Secondary Structure
MLKSNLNIDNRFSLIGIVASQPKRHQSPNGIYHCQFVLEHRSIQEEAGLSRQAWCKMPVQ
CCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEEEEECHHHHHHHCCCCCHHHCCCCCC
MSGNQFREKTQSITVGSQLLVVGFISSHKTANGLSQLVLHAEQIEFID
CCCHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100