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The map label for this gene is dut

Identifier: 15603019

GI number: 15603019

Start: 1351923

End: 1352378

Strand: Direct

Name: dut

Synonym: PM1154

Alternate gene names: 15603019

Gene position: 1351923-1352378 (Clockwise)

Preceding gene: 15603018

Following gene: 15603020

Centisome position: 59.89

GC content: 42.11

Gene sequence:

>456_bases
ATGAAAAAAATTGATGTCAAAATTCTCGATCAACGTATTGGAACAGAATTCCCTTTGCCAACATATGCAACCGAAGGGTC
CGCGGGCTTAGATTTAAGAGCATTAATTGATGCGCCAATGACTGTAGAAGCGGGACAAACCGTGTTAATTCCAACAGGTT
TATCTCTTTATATTGCCGATCCTACGCTGGCGGCTGTTATTTTGCCTCGTTCAGGTTTAGGTCATAAACATGGCATCGTT
TTAGGTAATTTAGTCGGTTTAATTGACTCGGATTATCAAGGTCCATTAATGGTTTCGCTGTGGAACCGTAGCACTGAGCC
ATTTAAAGTGGAAGTAGGTGATCGTATTGCCCAATTAGTGTTTGTGCCTGTCGTGCAAGCTGAGTTTAATGTCGTCAGTG
ATTTTGCACAAACTGAGCGTGGTGAAGGTGGATTTGGACATTCGGGTAAACAATAA

Upstream 100 bases:

>100_bases
CAGCACAAAATTAGCATTAGAGAGTAAAAAAACATTAGCCAAACAATTAGTTAATCAAATTATTGCGCGTTACCGCCAAT
CTTAAACAATAGGACAAAGA

Downstream 100 bases:

>100_bases
GCTATGCAAAAACCAGTAAAACGCAGTCTGAAAGAACGCCGTCAGCAAGTGCTGACCGTTCTGACACATATGTTGCATTC
GGAACGGGGAATGGAACGTA

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 151; Mature: 151

Protein sequence:

>151_residues
MKKIDVKILDQRIGTEFPLPTYATEGSAGLDLRALIDAPMTVEAGQTVLIPTGLSLYIADPTLAAVILPRSGLGHKHGIV
LGNLVGLIDSDYQGPLMVSLWNRSTEPFKVEVGDRIAQLVFVPVVQAEFNVVSDFAQTERGEGGFGHSGKQ

Sequences:

>Translated_151_residues
MKKIDVKILDQRIGTEFPLPTYATEGSAGLDLRALIDAPMTVEAGQTVLIPTGLSLYIADPTLAAVILPRSGLGHKHGIV
LGNLVGLIDSDYQGPLMVSLWNRSTEPFKVEVGDRIAQLVFVPVVQAEFNVVSDFAQTERGEGGFGHSGKQ
>Mature_151_residues
MKKIDVKILDQRIGTEFPLPTYATEGSAGLDLRALIDAPMTVEAGQTVLIPTGLSLYIADPTLAAVILPRSGLGHKHGIV
LGNLVGLIDSDYQGPLMVSLWNRSTEPFKVEVGDRIAQLVFVPVVQAEFNVVSDFAQTERGEGGFGHSGKQ

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI70906444, Length=141, Percent_Identity=37.5886524822695, Blast_Score=80, Evalue=9e-16,
Organism=Homo sapiens, GI4503423, Length=140, Percent_Identity=36.4285714285714, Blast_Score=79, Evalue=1e-15,
Organism=Homo sapiens, GI70906441, Length=141, Percent_Identity=37.5886524822695, Blast_Score=78, Evalue=3e-15,
Organism=Escherichia coli, GI1790071, Length=151, Percent_Identity=71.523178807947, Blast_Score=233, Evalue=3e-63,
Organism=Caenorhabditis elegans, GI71988561, Length=132, Percent_Identity=39.3939393939394, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6319729, Length=133, Percent_Identity=36.8421052631579, Blast_Score=76, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19921126, Length=148, Percent_Identity=37.1621621621622, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24583610, Length=148, Percent_Identity=37.1621621621622, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_PASMU (P57914)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246091.1
- ProteinModelPortal:   P57914
- SMR:   P57914
- GeneID:   1244501
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1154
- NMPDR:   fig|272843.1.peg.1154
- HOGENOM:   HBG436079
- OMA:   LDLRACI
- ProtClustDB:   PRK00601
- BioCyc:   PMUL272843:PM1154-MONOMER
- BRENDA:   3.6.1.23
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 16171; Mature: 16171

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: BINDING 83-83 BINDING 97-97

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIDVKILDQRIGTEFPLPTYATEGSAGLDLRALIDAPMTVEAGQTVLIPTGLSLYIAD
CCCCCCEEEHHHCCCCCCCCCCCCCCCCCCEEEEEECCCEEECCCCEEEEECCCEEEEEC
PTLAAVILPRSGLGHKHGIVLGNLVGLIDSDYQGPLMVSLWNRSTEPFKVEVGDRIAQLV
CCEEEEEECCCCCCCCCCEEEHHHHHHCCCCCCCCEEEEEECCCCCCEEEEHHHHHHHHH
FVPVVQAEFNVVSDFAQTERGEGGFGHSGKQ
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKKIDVKILDQRIGTEFPLPTYATEGSAGLDLRALIDAPMTVEAGQTVLIPTGLSLYIAD
CCCCCCEEEHHHCCCCCCCCCCCCCCCCCCEEEEEECCCEEECCCCEEEEECCCEEEEEC
PTLAAVILPRSGLGHKHGIVLGNLVGLIDSDYQGPLMVSLWNRSTEPFKVEVGDRIAQLV
CCEEEEEECCCCCCCCCCEEEHHHHHHCCCCCCCCEEEEEECCCCCCEEEEHHHHHHHHH
FVPVVQAEFNVVSDFAQTERGEGGFGHSGKQ
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100