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The map label for this gene is aspA [H]

Identifier: 15602968

GI number: 15602968

Start: 1297617

End: 1299035

Strand: Reverse

Name: aspA [H]

Synonym: PM1103

Alternate gene names: 15602968

Gene position: 1299035-1297617 (Counterclockwise)

Preceding gene: 15602977

Following gene: 15602962

Centisome position: 57.54

GC content: 39.89

Gene sequence:

>1419_bases
ATGACAGTAACAAGAAAAGAAGTGGATTTACTCGGAGAACGTGAAGTACCAGCAGACGCATACTGGGGAATTCATACCTT
AAGAGCGGTAGAAAATTTTAATATTTCGAATGCCGTTATCTCAGATGTGCCTGATTTTGTACGCGGTATGGTGATGGTGA
AAAAGGCGACCGCCTTAGCAAACGGTGAATTAGGGGCGATTCCTGATAAAATTGCAAAAGCGATTGTAGCTGCTTGTGAT
GAAATCTTAACCACAGGCAAATGCTTAGATCAATTCCCGTCAGATATTTATCAAGGCGGTGCAGGTACCTCAGTGAATAT
GAATACCAATGAGGTTGTGGCGAATTTGGCGTTAGAGTTATTAGGACATAAGAAAGGGGAATACCAATATTTAGATCCAA
TGGATCATGTTAACGCTAGCCAATCAACCAATGACGCCTATCCAACAGGTTTCCGTATTGCTGTCTATAACAGTATTATG
AAGCTCGTGGCAAAAGTGCTTTATTTACAACAAGGTTTTGAAAATAAAGCAAAAGAATTTTCGCACATTCTTAAAATGGG
ACGCACGCAATTACAAGATGCTGTGCCAATGACAGTAGGACAAGAATTTAAAGCCTTCTCTGTTTTATTAGATGAAGAAG
TTCGCAATTTAACACGTACAGCAGAATACTTATTAGAAGTTAATTTAGGTGCGACAGCAATTGGTACGGGCTTAAATACA
CCAAAAGGGTATGCGCCATTAGCGGTCAAATACCTGTCTGAAGTCACAGGATTACCTTGTGTATTAGCTGAAAACTTAAT
CGAAGCTACCTCGGATTGTGGGGCGTATGTGATGGTACACGGTGCATTAAAACGTACTGCGGTGAAACTATCAAAAGTAT
GTAATGACTTACGTCTATTATCTTCAGGTCCGCGTGCAGGGTTAAATGAGATTAATTTGCCTGAATTACAAGCGGGTTCA
TCGATTATGCCAGCAAAAGTCAACCCAGTGGTACCCGAAGTGGTTAACCAAGTTTGCTATAAAGTGATGGGGAACGATAC
TACCATTACCTTTGCCGCAGAGGCTGGACAATTACAATTAAATGTCATGGAGCCAGTGATTGCACAAGCGATGTTTGAGT
CTATTGATATTTTAACCAATGCTTGTGTGAACTTACGCGATAAATGTATTGATGGCATTACGGTGAATAAAGAAACTTGT
GAAAACTATGTCTTTAATTCTATCGGTATCGTGACTTATTTAAATCCATTTATTGGACACCATAATGGTGACTTAGTCGG
TAAAATCTGTGCGAAAACAGGGCGCGGAGTGAAAGAGGTCGTACTCGAAAAAGGACTCCTCACAGAAGCAGAATTAGATG
ATATTCTCTCAGTGGAGAACTTAATGAATCCAACCTATAAAGCGAAGTTGAATAAATAA

Upstream 100 bases:

>100_bases
GGGGGCAATCTGTAAAATGTGACGGCGATCAAATATTTAGCAAAGTTATTTCCATTATAATAGGCATTAACTTGTTCATC
ACTCGAAATGGAGGGTAATA

Downstream 100 bases:

>100_bases
CTTGCAAAATAACTGTTAATTTAACCGCACTTTGAGCTTGCTTAAAGTGCGGTTTCTTTTTTGGAAATTTTCTTTGTTAT
ACCTCGACAAGAAAAAATGG

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MTVTRKEVDLLGEREVPADAYWGIHTLRAVENFNISNAVISDVPDFVRGMVMVKKATALANGELGAIPDKIAKAIVAACD
EILTTGKCLDQFPSDIYQGGAGTSVNMNTNEVVANLALELLGHKKGEYQYLDPMDHVNASQSTNDAYPTGFRIAVYNSIM
KLVAKVLYLQQGFENKAKEFSHILKMGRTQLQDAVPMTVGQEFKAFSVLLDEEVRNLTRTAEYLLEVNLGATAIGTGLNT
PKGYAPLAVKYLSEVTGLPCVLAENLIEATSDCGAYVMVHGALKRTAVKLSKVCNDLRLLSSGPRAGLNEINLPELQAGS
SIMPAKVNPVVPEVVNQVCYKVMGNDTTITFAAEAGQLQLNVMEPVIAQAMFESIDILTNACVNLRDKCIDGITVNKETC
ENYVFNSIGIVTYLNPFIGHHNGDLVGKICAKTGRGVKEVVLEKGLLTEAELDDILSVENLMNPTYKAKLNK

Sequences:

>Translated_472_residues
MTVTRKEVDLLGEREVPADAYWGIHTLRAVENFNISNAVISDVPDFVRGMVMVKKATALANGELGAIPDKIAKAIVAACD
EILTTGKCLDQFPSDIYQGGAGTSVNMNTNEVVANLALELLGHKKGEYQYLDPMDHVNASQSTNDAYPTGFRIAVYNSIM
KLVAKVLYLQQGFENKAKEFSHILKMGRTQLQDAVPMTVGQEFKAFSVLLDEEVRNLTRTAEYLLEVNLGATAIGTGLNT
PKGYAPLAVKYLSEVTGLPCVLAENLIEATSDCGAYVMVHGALKRTAVKLSKVCNDLRLLSSGPRAGLNEINLPELQAGS
SIMPAKVNPVVPEVVNQVCYKVMGNDTTITFAAEAGQLQLNVMEPVIAQAMFESIDILTNACVNLRDKCIDGITVNKETC
ENYVFNSIGIVTYLNPFIGHHNGDLVGKICAKTGRGVKEVVLEKGLLTEAELDDILSVENLMNPTYKAKLNK
>Mature_471_residues
TVTRKEVDLLGEREVPADAYWGIHTLRAVENFNISNAVISDVPDFVRGMVMVKKATALANGELGAIPDKIAKAIVAACDE
ILTTGKCLDQFPSDIYQGGAGTSVNMNTNEVVANLALELLGHKKGEYQYLDPMDHVNASQSTNDAYPTGFRIAVYNSIMK
LVAKVLYLQQGFENKAKEFSHILKMGRTQLQDAVPMTVGQEFKAFSVLLDEEVRNLTRTAEYLLEVNLGATAIGTGLNTP
KGYAPLAVKYLSEVTGLPCVLAENLIEATSDCGAYVMVHGALKRTAVKLSKVCNDLRLLSSGPRAGLNEINLPELQAGSS
IMPAKVNPVVPEVVNQVCYKVMGNDTTITFAAEAGQLQLNVMEPVIAQAMFESIDILTNACVNLRDKCIDGITVNKETCE
NYVFNSIGIVTYLNPFIGHHNGDLVGKICAKTGRGVKEVVLEKGLLTEAELDDILSVENLMNPTYKAKLNK

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=461, Percent_Identity=38.6117136659436, Blast_Score=322, Evalue=5e-88,
Organism=Escherichia coli, GI87082375, Length=465, Percent_Identity=80, Blast_Score=762, Evalue=0.0,
Organism=Escherichia coli, GI1787896, Length=463, Percent_Identity=38.8768898488121, Blast_Score=331, Evalue=8e-92,
Organism=Caenorhabditis elegans, GI17553882, Length=465, Percent_Identity=41.7204301075269, Blast_Score=361, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI32565146, Length=326, Percent_Identity=41.4110429447853, Blast_Score=262, Evalue=3e-70,
Organism=Saccharomyces cerevisiae, GI6324993, Length=461, Percent_Identity=41.8655097613883, Blast_Score=352, Evalue=8e-98,
Organism=Drosophila melanogaster, GI24662684, Length=465, Percent_Identity=40.6451612903226, Blast_Score=345, Evalue=4e-95,
Organism=Drosophila melanogaster, GI24640179, Length=463, Percent_Identity=40.1727861771058, Blast_Score=336, Evalue=2e-92,
Organism=Drosophila melanogaster, GI24640177, Length=463, Percent_Identity=40.1727861771058, Blast_Score=336, Evalue=3e-92,
Organism=Drosophila melanogaster, GI78710009, Length=463, Percent_Identity=39.524838012959, Blast_Score=334, Evalue=1e-91,
Organism=Drosophila melanogaster, GI24583245, Length=449, Percent_Identity=33.630289532294, Blast_Score=273, Evalue=2e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 51165; Mature: 51033

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVTRKEVDLLGEREVPADAYWGIHTLRAVENFNISNAVISDVPDFVRGMVMVKKATALA
CCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
NGELGAIPDKIAKAIVAACDEILTTGKCLDQFPSDIYQGGAGTSVNMNTNEVVANLALEL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCEECCCHHHHHHHHHHHH
LGHKKGEYQYLDPMDHVNASQSTNDAYPTGFRIAVYNSIMKLVAKVLYLQQGFENKAKEF
HCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
SHILKMGRTQLQDAVPMTVGQEFKAFSVLLDEEVRNLTRTAEYLLEVNLGATAIGTGLNT
HHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCC
PKGYAPLAVKYLSEVTGLPCVLAENLIEATSDCGAYVMVHGALKRTAVKLSKVCNDLRLL
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
SSGPRAGLNEINLPELQAGSSIMPAKVNPVVPEVVNQVCYKVMGNDTTITFAAEAGQLQL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCEEE
NVMEPVIAQAMFESIDILTNACVNLRDKCIDGITVNKETCENYVFNSIGIVTYLNPFIGH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
HNGDLVGKICAKTGRGVKEVVLEKGLLTEAELDDILSVENLMNPTYKAKLNK
CCCCHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHCCCCC
>Mature Secondary Structure 
TVTRKEVDLLGEREVPADAYWGIHTLRAVENFNISNAVISDVPDFVRGMVMVKKATALA
CCCHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
NGELGAIPDKIAKAIVAACDEILTTGKCLDQFPSDIYQGGAGTSVNMNTNEVVANLALEL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCEECCCHHHHHHHHHHHH
LGHKKGEYQYLDPMDHVNASQSTNDAYPTGFRIAVYNSIMKLVAKVLYLQQGFENKAKEF
HCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
SHILKMGRTQLQDAVPMTVGQEFKAFSVLLDEEVRNLTRTAEYLLEVNLGATAIGTGLNT
HHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCC
PKGYAPLAVKYLSEVTGLPCVLAENLIEATSDCGAYVMVHGALKRTAVKLSKVCNDLRLL
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
SSGPRAGLNEINLPELQAGSSIMPAKVNPVVPEVVNQVCYKVMGNDTTITFAAEAGQLQL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCEEE
NVMEPVIAQAMFESIDILTNACVNLRDKCIDGITVNKETCENYVFNSIGIVTYLNPFIGH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
HNGDLVGKICAKTGRGVKEVVLEKGLLTEAELDDILSVENLMNPTYKAKLNK
CCCCHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]