Click here to switch to the map view.

The map label for this gene is rep [H]

Identifier: 15602924

GI number: 15602924

Start: 1246831

End: 1248846

Strand: Reverse

Name: rep [H]

Synonym: PM1059

Alternate gene names: 15602924

Gene position: 1248846-1246831 (Counterclockwise)

Preceding gene: 15602925

Following gene: 15602922

Centisome position: 55.32

GC content: 40.92

Gene sequence:

>2016_bases
ATGAAATTAAATAAACAGCAACAACAAGCCGTGGAATATGTCACTGGACCTTGCTTAGTGCTCGCTGGGGCGGGCTCGGG
TAAAACACGGGTTATCATTAATAAAATTGCTTATCTCGTTGCCAAATGTGGCTATGTTCCGCGCCAGATTGCGGCGGTGA
CTTTTACGAACAAAGCTGCACGGGAAATGAAAGAACGTGTTGCCCACTCAATTGGTAAAGAAGCCAGTCGTGGTCTAATT
GTATCCACGTTTCACACTTTAGGCTTTGATATGATTAAACGTGAATATAAGCAATTAGGCTTTAAAGCGAATATGACGTT
GTTTGATGAACATGATCAATATGCGCTATTAAAAGAGTTAACAGCGGACTTATTGTGTGAGGACAAGGATTTATTACGTG
AATTAATTTCGGTCATTTCTCGTTGGAAAAATGATTTAGTTTTGCCACCACAGGCAAAACAGCTTGCACGTGATCACAAG
CAACAAATGTTTGCAGAGTGTTATGATCGTTATAACCAGCAAATTCGTGCATACAATGCATTGGATTTTGACGATTTAAT
CATGTTACCGACCTTATTATTACAACAAAATGATGCGGTTCGCACGAAGTGGCAACAGAAAATCCGCTATTTATTAGTCG
ATGAATATCAGGATACCAATACCAGTCAGTATGAATTGATCAAATTATTAGTCGGGGAGCGCGCTTGCTTTACGGTAGTC
GGAGATGATGATCAATCAATTTATTCTTGGCGTGGCGCGCAGCCACAAAATATGATGCGGTTGAAAACCGATTTTCCTCA
CTTAAACGTCGTGAAATTAGAACAAAATTATCGTTCAACACAACGCATTTTACATTGTGCCAATATTTTGATCGATAATA
ATGATCATGTGTTTGCTAAAAAACTGTTTTCAACACTAGATCAAGGGGAAAAGCTACAAGTTATCGAAGCAAAAAATGAA
GAAGAAGAAGCGGAACGTGTGGTAGGGGAATTAATCGCACACCGATTTATGCGCAAAACCAAGTATAAAGATTATGCGAT
TTTATATCGCGGTAATCATCAATCGCGCTTGTTGGAAAAGGTGTTAATGCAAAACCGTATTCCCTACAAAATTTCAGGGG
GGACCTCCTTTTTCTCGCGTGCAGAAATTAAAGACATGATGGCGTATTTACGTTTAGTGGTAAATCAAGATGATGACGCC
GCGTTTTTACGTATTGTGAATACGCCTAAGCGCGAAATTGGGACGGTGACGTTACAAAAATTAGGTGAGTTAGCCCATGA
AAAACATTGTAGTTTATTTGAAGCAATTTTTGATTTTGAGTTAATTCAACGAATTACACCTAAAGCCTATAATGCGTTGC
AACATTTCGGACAGTGGATCGTGCAATTAAGTGATGAGGTAATTCGCTCAGAACCTGAACGCGCGATTCGTAGCATGCTC
GCACAACTTCATTACGAAGAATACTTATACGAATATGCGACCAGTCCTAAAGCAGCGGAAATGCAAAGTAAAAATGTGGC
GACACTGTTTGATTGGGTCGCGGAGATGTTAAAGGGAAATGACATGGACGAACCAATTACGCTTAATCAAGTGGTGACAC
GTTTGACATTACGTGACATGTTGGAGCGAGGAGAAGAGGATGATGAAAGTGATCAAGTGCAATTAATGACGCTACATGCT
TCCAAAGGCTTAGAATTTCCGCATGTTTTTTTAATTGGAATGGAAGAAGGGATTTTACCGCATCAGACCAGTATTGATGA
GGATAATGTGGAAGAAGAACGCCGTTTAGCCTATGTAGGCATTACGCGTGCGCAACAAACATTAACCTTCTCGTTATGCA
AAGAAAGACGCCAGTTTGGTGAGTTACTAAAGCCTGAGCCAAGTCGCTTTTTGGCTGAACTACCCACCGATGATGTGCAG
TGGGAGCGCGATAAGCCACCTTTAACACAGGAACAAGTTCGCGAGAATACCGCCACACAATTGGCGAACTTACGTGCGAT
TTTGCGGGGTAAATAG

Upstream 100 bases:

>100_bases
AAGCGCTGGGGACTTCCTATTTTAGATTTGCCTTTCTCTTTTATATTGGATACCGCATTATTGCCTTATGTCTGGACTCA
AGAAACCGAATAAATGACCC

Downstream 100 bases:

>100_bases
TTTTTAAGTGGGTATTTTATTGAATTTGTAGAGAAATCAGTCAACTGAATAATTTTTTTGTAAAAATGATTTGACTTATT
TTTAGAAAAACGTATTATAC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 671; Mature: 671

Protein sequence:

>671_residues
MKLNKQQQQAVEYVTGPCLVLAGAGSGKTRVIINKIAYLVAKCGYVPRQIAAVTFTNKAAREMKERVAHSIGKEASRGLI
VSTFHTLGFDMIKREYKQLGFKANMTLFDEHDQYALLKELTADLLCEDKDLLRELISVISRWKNDLVLPPQAKQLARDHK
QQMFAECYDRYNQQIRAYNALDFDDLIMLPTLLLQQNDAVRTKWQQKIRYLLVDEYQDTNTSQYELIKLLVGERACFTVV
GDDDQSIYSWRGAQPQNMMRLKTDFPHLNVVKLEQNYRSTQRILHCANILIDNNDHVFAKKLFSTLDQGEKLQVIEAKNE
EEEAERVVGELIAHRFMRKTKYKDYAILYRGNHQSRLLEKVLMQNRIPYKISGGTSFFSRAEIKDMMAYLRLVVNQDDDA
AFLRIVNTPKREIGTVTLQKLGELAHEKHCSLFEAIFDFELIQRITPKAYNALQHFGQWIVQLSDEVIRSEPERAIRSML
AQLHYEEYLYEYATSPKAAEMQSKNVATLFDWVAEMLKGNDMDEPITLNQVVTRLTLRDMLERGEEDDESDQVQLMTLHA
SKGLEFPHVFLIGMEEGILPHQTSIDEDNVEEERRLAYVGITRAQQTLTFSLCKERRQFGELLKPEPSRFLAELPTDDVQ
WERDKPPLTQEQVRENTATQLANLRAILRGK

Sequences:

>Translated_671_residues
MKLNKQQQQAVEYVTGPCLVLAGAGSGKTRVIINKIAYLVAKCGYVPRQIAAVTFTNKAAREMKERVAHSIGKEASRGLI
VSTFHTLGFDMIKREYKQLGFKANMTLFDEHDQYALLKELTADLLCEDKDLLRELISVISRWKNDLVLPPQAKQLARDHK
QQMFAECYDRYNQQIRAYNALDFDDLIMLPTLLLQQNDAVRTKWQQKIRYLLVDEYQDTNTSQYELIKLLVGERACFTVV
GDDDQSIYSWRGAQPQNMMRLKTDFPHLNVVKLEQNYRSTQRILHCANILIDNNDHVFAKKLFSTLDQGEKLQVIEAKNE
EEEAERVVGELIAHRFMRKTKYKDYAILYRGNHQSRLLEKVLMQNRIPYKISGGTSFFSRAEIKDMMAYLRLVVNQDDDA
AFLRIVNTPKREIGTVTLQKLGELAHEKHCSLFEAIFDFELIQRITPKAYNALQHFGQWIVQLSDEVIRSEPERAIRSML
AQLHYEEYLYEYATSPKAAEMQSKNVATLFDWVAEMLKGNDMDEPITLNQVVTRLTLRDMLERGEEDDESDQVQLMTLHA
SKGLEFPHVFLIGMEEGILPHQTSIDEDNVEEERRLAYVGITRAQQTLTFSLCKERRQFGELLKPEPSRFLAELPTDDVQ
WERDKPPLTQEQVRENTATQLANLRAILRGK
>Mature_671_residues
MKLNKQQQQAVEYVTGPCLVLAGAGSGKTRVIINKIAYLVAKCGYVPRQIAAVTFTNKAAREMKERVAHSIGKEASRGLI
VSTFHTLGFDMIKREYKQLGFKANMTLFDEHDQYALLKELTADLLCEDKDLLRELISVISRWKNDLVLPPQAKQLARDHK
QQMFAECYDRYNQQIRAYNALDFDDLIMLPTLLLQQNDAVRTKWQQKIRYLLVDEYQDTNTSQYELIKLLVGERACFTVV
GDDDQSIYSWRGAQPQNMMRLKTDFPHLNVVKLEQNYRSTQRILHCANILIDNNDHVFAKKLFSTLDQGEKLQVIEAKNE
EEEAERVVGELIAHRFMRKTKYKDYAILYRGNHQSRLLEKVLMQNRIPYKISGGTSFFSRAEIKDMMAYLRLVVNQDDDA
AFLRIVNTPKREIGTVTLQKLGELAHEKHCSLFEAIFDFELIQRITPKAYNALQHFGQWIVQLSDEVIRSEPERAIRSML
AQLHYEEYLYEYATSPKAAEMQSKNVATLFDWVAEMLKGNDMDEPITLNQVVTRLTLRDMLERGEEDDESDQVQLMTLHA
SKGLEFPHVFLIGMEEGILPHQTSIDEDNVEEERRLAYVGITRAQQTLTFSLCKERRQFGELLKPEPSRFLAELPTDDVQ
WERDKPPLTQEQVRENTATQLANLRAILRGK

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=671, Percent_Identity=65.8718330849478, Blast_Score=911, Evalue=0.0,
Organism=Escherichia coli, GI2367296, Length=645, Percent_Identity=38.1395348837209, Blast_Score=407, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6322369, Length=739, Percent_Identity=27.1989174560216, Blast_Score=196, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6324477, Length=464, Percent_Identity=22.8448275862069, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 77713; Mature: 77713

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNKQQQQAVEYVTGPCLVLAGAGSGKTRVIINKIAYLVAKCGYVPRQIAAVTFTNKAA
CCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHEEECHHHH
REMKERVAHSIGKEASRGLIVSTFHTLGFDMIKREYKQLGFKANMTLFDEHDQYALLKEL
HHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHH
TADLLCEDKDLLRELISVISRWKNDLVLPPQAKQLARDHKQQMFAECYDRYNQQIRAYNA
HHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LDFDDLIMLPTLLLQQNDAVRTKWQQKIRYLLVDEYQDTNTSQYELIKLLVGERACFTVV
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEE
GDDDQSIYSWRGAQPQNMMRLKTDFPHLNVVKLEQNYRSTQRILHCANILIDNNDHVFAK
CCCCCHHHHCCCCCCHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCHHHH
KLFSTLDQGEKLQVIEAKNEEEEAERVVGELIAHRFMRKTKYKDYAILYRGNHQSRLLEK
HHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHH
VLMQNRIPYKISGGTSFFSRAEIKDMMAYLRLVVNQDDDAAFLRIVNTPKREIGTVTLQK
HHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHH
LGELAHEKHCSLFEAIFDFELIQRITPKAYNALQHFGQWIVQLSDEVIRSEPERAIRSML
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
AQLHYEEYLYEYATSPKAAEMQSKNVATLFDWVAEMLKGNDMDEPITLNQVVTRLTLRDM
HHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHH
LERGEEDDESDQVQLMTLHASKGLEFPHVFLIGMEEGILPHQTSIDEDNVEEERRLAYVG
HHCCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHEE
ITRAQQTLTFSLCKERRQFGELLKPEPSRFLAELPTDDVQWERDKPPLTQEQVRENTATQ
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHH
LANLRAILRGK
HHHHHHHHCCC
>Mature Secondary Structure
MKLNKQQQQAVEYVTGPCLVLAGAGSGKTRVIINKIAYLVAKCGYVPRQIAAVTFTNKAA
CCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHEEECHHHH
REMKERVAHSIGKEASRGLIVSTFHTLGFDMIKREYKQLGFKANMTLFDEHDQYALLKEL
HHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHH
TADLLCEDKDLLRELISVISRWKNDLVLPPQAKQLARDHKQQMFAECYDRYNQQIRAYNA
HHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LDFDDLIMLPTLLLQQNDAVRTKWQQKIRYLLVDEYQDTNTSQYELIKLLVGERACFTVV
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEE
GDDDQSIYSWRGAQPQNMMRLKTDFPHLNVVKLEQNYRSTQRILHCANILIDNNDHVFAK
CCCCCHHHHCCCCCCHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCHHHH
KLFSTLDQGEKLQVIEAKNEEEEAERVVGELIAHRFMRKTKYKDYAILYRGNHQSRLLEK
HHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHH
VLMQNRIPYKISGGTSFFSRAEIKDMMAYLRLVVNQDDDAAFLRIVNTPKREIGTVTLQK
HHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHH
LGELAHEKHCSLFEAIFDFELIQRITPKAYNALQHFGQWIVQLSDEVIRSEPERAIRSML
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
AQLHYEEYLYEYATSPKAAEMQSKNVATLFDWVAEMLKGNDMDEPITLNQVVTRLTLRDM
HHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHH
LERGEEDDESDQVQLMTLHASKGLEFPHVFLIGMEEGILPHQTSIDEDNVEEERRLAYVG
HHCCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHEE
ITRAQQTLTFSLCKERRQFGELLKPEPSRFLAELPTDDVQWERDKPPLTQEQVRENTATQ
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHH
LANLRAILRGK
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]