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The map label for this gene is purT

Identifier: 15602918

GI number: 15602918

Start: 1242122

End: 1243303

Strand: Reverse

Name: purT

Synonym: PM1053

Alternate gene names: 15602918

Gene position: 1243303-1242122 (Counterclockwise)

Preceding gene: 15602919

Following gene: 15602916

Centisome position: 55.07

GC content: 45.6

Gene sequence:

>1182_bases
ATGACAACAATTGGTACCCCACTTACGCCGAAAGCCACAAAAGTGATGATGCTAGGTTCAGGCGAACTCGGTAAAGAAGT
GGTGATTGAGCTACAACGCCTTGGTGTTGAGGTGATTGCTGTTGATCGTTATGCTAATGCGCCAGCACAACAAGTGGCAC
ATCGTGCTTATACGATATCTATGCTTGATGGCGAGGCTTTAAAAGCCTTGGTAGAGAAAGAAAAGCCAGATTATATCGTG
CCAGAAGTGGAAGCGATTGCGACGGCAACGCTCGTGGAATTAGAGCAGGCAGGGTTTACTGTGATTCCAACGGCGAAAGC
AACCCAATTAACCATGAATCGTGAAGGTATTCGTCGTTTGGCAGCAGAAGAGTTAGGGTTACCAACGTCAAACTACCAAT
TTGTTGATAATTTTGATGATTTCAAACGCGCAGTGGAAAAAATTGGGATCCCTTGTGTGGTTAAGCCGATTATGTCTTCT
TCAGGACATGGACAAAGTATTTTGAAATCAGTCGAACAGATACCGTCCGCGTGGGAATATGCGCAACAAGGTGGGCGTGC
GGGGGCAGGACGTGTCATTGTGGAAGGTTTTGTCAAATTTGATTATGAAATTACGTTATTAACAGTACGTCATGTCCATG
GCACCTCTTTTCTCGCACCAATCGGTCACATTCAAATTGATGGGGATTATCGCGAATCTTGGCAACCTCAAGCCATGTCA
GCGGTGGCATTAGAAAAAGCGCAGGCGATTGCAGAAAAAATTACTGGTGCGTTAGGGGGACGTGGTATTTTTGGCGTGGA
AATGTTTGTTTGTGGGGATGAGGTCATTTTCAATGAGGTTTCACCACGCCCTCATGATACTGGCATGGTAACCTTAATTT
CGCAAGAATTGTCTGAGTTTGCGTTACACGCACGAGCTATTTTAGGTTTGCCAATTCCGGACATTACGCTTATTAGCCCG
TCAGCGTCCAAAGCGATTGTGGTTGAGGGGAAATCCACGCAAGTCGTATTTGGTCAATTGGCTGAGGTGCTTGCTGAACC
GCATACGAATCTTCGTTTATTTGGTAAGGGCGAAGTAGATGGTCACCGTCGTATGGGCGTGATTTTAGCACGTGATATTT
CTGTTGAGAAAGCATTAGAAAAAGCACGCCGTGCTTATGATAAGCTTGAGATTGCGCTGTAA

Upstream 100 bases:

>100_bases
CGTGGTGTGAGTGTGAATAAAAATGAAGATTTATTACTCGACGAACAATTATTATCTCTTTAAGCATTTATAAAACACAA
CTGTCCAGAAGGAAATCCAT

Downstream 100 bases:

>100_bases
AGGTTCTGTGATAGCAATGACCGCGCTGTTAAGTGCGGTCGTTTTTGTCTTTATTTTTGAGTTAAATAATCTAACACCAC
TTGATGATGGTTTGAGGTTT

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MTTIGTPLTPKATKVMMLGSGELGKEVVIELQRLGVEVIAVDRYANAPAQQVAHRAYTISMLDGEALKALVEKEKPDYIV
PEVEAIATATLVELEQAGFTVIPTAKATQLTMNREGIRRLAAEELGLPTSNYQFVDNFDDFKRAVEKIGIPCVVKPIMSS
SGHGQSILKSVEQIPSAWEYAQQGGRAGAGRVIVEGFVKFDYEITLLTVRHVHGTSFLAPIGHIQIDGDYRESWQPQAMS
AVALEKAQAIAEKITGALGGRGIFGVEMFVCGDEVIFNEVSPRPHDTGMVTLISQELSEFALHARAILGLPIPDITLISP
SASKAIVVEGKSTQVVFGQLAEVLAEPHTNLRLFGKGEVDGHRRMGVILARDISVEKALEKARRAYDKLEIAL

Sequences:

>Translated_393_residues
MTTIGTPLTPKATKVMMLGSGELGKEVVIELQRLGVEVIAVDRYANAPAQQVAHRAYTISMLDGEALKALVEKEKPDYIV
PEVEAIATATLVELEQAGFTVIPTAKATQLTMNREGIRRLAAEELGLPTSNYQFVDNFDDFKRAVEKIGIPCVVKPIMSS
SGHGQSILKSVEQIPSAWEYAQQGGRAGAGRVIVEGFVKFDYEITLLTVRHVHGTSFLAPIGHIQIDGDYRESWQPQAMS
AVALEKAQAIAEKITGALGGRGIFGVEMFVCGDEVIFNEVSPRPHDTGMVTLISQELSEFALHARAILGLPIPDITLISP
SASKAIVVEGKSTQVVFGQLAEVLAEPHTNLRLFGKGEVDGHRRMGVILARDISVEKALEKARRAYDKLEIAL
>Mature_392_residues
TTIGTPLTPKATKVMMLGSGELGKEVVIELQRLGVEVIAVDRYANAPAQQVAHRAYTISMLDGEALKALVEKEKPDYIVP
EVEAIATATLVELEQAGFTVIPTAKATQLTMNREGIRRLAAEELGLPTSNYQFVDNFDDFKRAVEKIGIPCVVKPIMSSS
GHGQSILKSVEQIPSAWEYAQQGGRAGAGRVIVEGFVKFDYEITLLTVRHVHGTSFLAPIGHIQIDGDYRESWQPQAMSA
VALEKAQAIAEKITGALGGRGIFGVEMFVCGDEVIFNEVSPRPHDTGMVTLISQELSEFALHARAILGLPIPDITLISPS
ASKAIVVEGKSTQVVFGQLAEVLAEPHTNLRLFGKGEVDGHRRMGVILARDISVEKALEKARRAYDKLEIAL

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1788155, Length=391, Percent_Identity=64.4501278772378, Blast_Score=512, Evalue=1e-146,
Organism=Escherichia coli, GI1786733, Length=203, Percent_Identity=28.5714285714286, Blast_Score=79, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6324702, Length=366, Percent_Identity=27.5956284153005, Blast_Score=109, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURT_PASMU (Q9CLZ5)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245990.1
- HSSP:   P33221
- ProteinModelPortal:   Q9CLZ5
- SMR:   Q9CLZ5
- GeneID:   1244400
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1053
- NMPDR:   fig|272843.1.peg.1053
- HOGENOM:   HBG516369
- OMA:   PDYIVPE
- ProtClustDB:   PRK09288
- BioCyc:   PMUL272843:PM1053-MONOMER
- HAMAP:   MF_01643
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF2
- TIGRFAMs:   TIGR01142

Pfam domain/function: PF02222 ATP-grasp; SSF52440 PreATP-grasp-like

EC number: 2.1.2.- [C]

Molecular weight: Translated: 42592; Mature: 42461

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 82-82 BINDING 114-114 BINDING 155-155 BINDING 203-203 BINDING 267-267 BINDING 279-279 BINDING 286-286 BINDING 356-356

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTIGTPLTPKATKVMMLGSGELGKEVVIELQRLGVEVIAVDRYANAPAQQVAHRAYTIS
CCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHEEEE
MLDGEALKALVEKEKPDYIVPEVEAIATATLVELEQAGFTVIPTAKATQLTMNREGIRRL
EECHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHCCEEEECCCCCEEEEECHHHHHHH
AAEELGLPTSNYQFVDNFDDFKRAVEKIGIPCVVKPIMSSSGHGQSILKSVEQIPSAWEY
HHHHHCCCCCCCEECCCHHHHHHHHHHCCCCEEEHHHHCCCCCCHHHHHHHHHCCHHHHH
AQQGGRAGAGRVIVEGFVKFDYEITLLTVRHVHGTSFLAPIGHIQIDGDYRESWQPQAMS
HHCCCCCCCCHHEEEEEEEECEEEEEEEEEEECCCCEECCCCEEEECCCCCCCCCCHHHH
AVALEKAQAIAEKITGALGGRGIFGVEMFVCGDEVIFNEVSPRPHDTGMVTLISQELSEF
HHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCHHEECCCCCCCCCCCEEEHHHHHHHHH
ALHARAILGLPIPDITLISPSASKAIVVEGKSTQVVFGQLAEVLAEPHTNLRLFGKGEVD
HHHHHHHCCCCCCCEEEECCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCCC
GHRRMGVILARDISVEKALEKARRAYDKLEIAL
CCCEEEEEEEECCCHHHHHHHHHHHHHHHEECC
>Mature Secondary Structure 
TTIGTPLTPKATKVMMLGSGELGKEVVIELQRLGVEVIAVDRYANAPAQQVAHRAYTIS
CCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHEEEE
MLDGEALKALVEKEKPDYIVPEVEAIATATLVELEQAGFTVIPTAKATQLTMNREGIRRL
EECHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHCCEEEECCCCCEEEEECHHHHHHH
AAEELGLPTSNYQFVDNFDDFKRAVEKIGIPCVVKPIMSSSGHGQSILKSVEQIPSAWEY
HHHHHCCCCCCCEECCCHHHHHHHHHHCCCCEEEHHHHCCCCCCHHHHHHHHHCCHHHHH
AQQGGRAGAGRVIVEGFVKFDYEITLLTVRHVHGTSFLAPIGHIQIDGDYRESWQPQAMS
HHCCCCCCCCHHEEEEEEEECEEEEEEEEEEECCCCEECCCCEEEECCCCCCCCCCHHHH
AVALEKAQAIAEKITGALGGRGIFGVEMFVCGDEVIFNEVSPRPHDTGMVTLISQELSEF
HHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCHHEECCCCCCCCCCCEEEHHHHHHHHH
ALHARAILGLPIPDITLISPSASKAIVVEGKSTQVVFGQLAEVLAEPHTNLRLFGKGEVD
HHHHHHHCCCCCCCEEEECCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCCC
GHRRMGVILARDISVEKALEKARRAYDKLEIAL
CCCEEEEEEEECCCHHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100