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The map label for this gene is miaB [H]

Identifier: 15602866

GI number: 15602866

Start: 1179742

End: 1181166

Strand: Reverse

Name: miaB [H]

Synonym: PM1001

Alternate gene names: 15602866

Gene position: 1181166-1179742 (Counterclockwise)

Preceding gene: 15602884

Following gene: 15602865

Centisome position: 52.32

GC content: 41.33

Gene sequence:

>1425_bases
ATGACGCAAAAATTACATATTAAAACGTGGGGTTGTCAGATGAATGAATATGATTCATCTAAAATGGCAGATCTCTTAAA
CAGTACTCACGGCTTAGAGTTAACAGAAATTCCGGAAGAAGCGGATGTGTTATTGTTAAACACTTGCTCAATTCGTGAAA
AAGCACAAGAAAAAGTTTTCCATCAATTAGGACGTTGGAAAGAATTAAAGAAACATAAGCCGGGACTCGTTATCGGTGTT
GGGGGCTGTGTTGCCTCACAAGAAGGAGAACACATTCGTACTCGTGCTCCTTATGTCGATATTATTTTTGGACCACAAAC
CTTACATCGTTTACCTGAAATGATCAATCAGATCAGAGGTGGTAAAAGCTCAGTAGTCGATGTCAGTTTCCCAGAAATTG
AAAAATTCGATCGTTTACCAGAACCGCGTGCAGAAGGTCCAACTGCTTTCGTATCCATTATGGAAGGCTGTAATAAATAT
TGCTCATTCTGTGTCGTGCCTTATACGCGTGGTGAAGAAGTCAGTCGTCCAGTGGATGATGTATTATTTGAAATTGCACA
GTTGGCAGAGCAAGGCGTGCGTGAAGTGAATTTATTAGGACAAAACGTGAACGCTTATCGTGGTGCAACTCATGATGACG
GTATTTGTACTTTTGCGGAATTGTTACGTTTAGTAGCCGCTATTGATGGTATCGACCGTTTACGTTTTACCACCAGTCAC
CCAATTGAGTTCACTGACGACATTATTGATGTGTACCGTGATACGCCAGAGTTGGTGAGCTTCTTACACTTACCTGTACA
AAGTGGTTCTGATCGTGTGTTATCTATGATGAAACGCAATCATACAGCATTAGAATATAAATCGATTATTCGGAAGTTAA
GAGCGGTGCGTCCAGAGATTCAAATTAGCTCAGATTTTATTGTTGGTTTCCCGGGTGAAACAGCAGAAGATTTTGAGCAA
ACCATGAATTTAATTGCACAAGTAAATTTTGATATGAGTTTCAGCTTCATTTATTCAGCACGTCCAGGCACGCCAGCAGC
AGATATGCCTGATGATGTGACGGAAGAAGAGAAGAAACAACGTTTATATGTGTTGCAACAACGCATTAACAATCAAGCCG
CGCAATTTAGTCGAGCAATGTTAGGCACAGAACAGCGCGTGTTAGTGGAAGGACCATCGAAAAAAGATTTAATGGAACTC
ACAGGGCGTACAGAAACTAATCGTATCGTCAATTTTGTGGGTACGCCAGATATGATTGGGAAGTTTGTTGATATCAAGAT
CACGGATGTGTTTACTAACTCACTGCGTGGTGAAGTCGTCAGAACTGAAGAACAAATGGGACTTCGCGTTGTTCAATCGC
CACAAATGGTGATTAATCGTACTCGTAAAGAAGATGAACTCGGCGTGGGACGTTATCACGCGTAG

Upstream 100 bases:

>100_bases
GAATTTCAAAAGAAATCTCTTTTTTAGCTAGCCAGCATAGGTTCAAGACTGTAAAATAGCCAGTCACATTTTTATAGGTT
AACTGAATTTTTTAAACGAT

Downstream 100 bases:

>100_bases
TAGTGCTATCCCTTCAAATATTTAACCGCTCTCGAGTTTCTCAAGAGCGGTTATTTTTTATGAAAAATTTTTGATAAATT
GACCGCTCTTTTTATTGCTA

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MTQKLHIKTWGCQMNEYDSSKMADLLNSTHGLELTEIPEEADVLLLNTCSIREKAQEKVFHQLGRWKELKKHKPGLVIGV
GGCVASQEGEHIRTRAPYVDIIFGPQTLHRLPEMINQIRGGKSSVVDVSFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
CSFCVVPYTRGEEVSRPVDDVLFEIAQLAEQGVREVNLLGQNVNAYRGATHDDGICTFAELLRLVAAIDGIDRLRFTTSH
PIEFTDDIIDVYRDTPELVSFLHLPVQSGSDRVLSMMKRNHTALEYKSIIRKLRAVRPEIQISSDFIVGFPGETAEDFEQ
TMNLIAQVNFDMSFSFIYSARPGTPAADMPDDVTEEEKKQRLYVLQQRINNQAAQFSRAMLGTEQRVLVEGPSKKDLMEL
TGRTETNRIVNFVGTPDMIGKFVDIKITDVFTNSLRGEVVRTEEQMGLRVVQSPQMVINRTRKEDELGVGRYHA

Sequences:

>Translated_474_residues
MTQKLHIKTWGCQMNEYDSSKMADLLNSTHGLELTEIPEEADVLLLNTCSIREKAQEKVFHQLGRWKELKKHKPGLVIGV
GGCVASQEGEHIRTRAPYVDIIFGPQTLHRLPEMINQIRGGKSSVVDVSFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
CSFCVVPYTRGEEVSRPVDDVLFEIAQLAEQGVREVNLLGQNVNAYRGATHDDGICTFAELLRLVAAIDGIDRLRFTTSH
PIEFTDDIIDVYRDTPELVSFLHLPVQSGSDRVLSMMKRNHTALEYKSIIRKLRAVRPEIQISSDFIVGFPGETAEDFEQ
TMNLIAQVNFDMSFSFIYSARPGTPAADMPDDVTEEEKKQRLYVLQQRINNQAAQFSRAMLGTEQRVLVEGPSKKDLMEL
TGRTETNRIVNFVGTPDMIGKFVDIKITDVFTNSLRGEVVRTEEQMGLRVVQSPQMVINRTRKEDELGVGRYHA
>Mature_473_residues
TQKLHIKTWGCQMNEYDSSKMADLLNSTHGLELTEIPEEADVLLLNTCSIREKAQEKVFHQLGRWKELKKHKPGLVIGVG
GCVASQEGEHIRTRAPYVDIIFGPQTLHRLPEMINQIRGGKSSVVDVSFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYC
SFCVVPYTRGEEVSRPVDDVLFEIAQLAEQGVREVNLLGQNVNAYRGATHDDGICTFAELLRLVAAIDGIDRLRFTTSHP
IEFTDDIIDVYRDTPELVSFLHLPVQSGSDRVLSMMKRNHTALEYKSIIRKLRAVRPEIQISSDFIVGFPGETAEDFEQT
MNLIAQVNFDMSFSFIYSARPGTPAADMPDDVTEEEKKQRLYVLQQRINNQAAQFSRAMLGTEQRVLVEGPSKKDLMELT
GRTETNRIVNFVGTPDMIGKFVDIKITDVFTNSLRGEVVRTEEQMGLRVVQSPQMVINRTRKEDELGVGRYHA

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=478, Percent_Identity=37.2384937238494, Blast_Score=294, Evalue=1e-79,
Organism=Homo sapiens, GI28872782, Length=478, Percent_Identity=37.2384937238494, Blast_Score=294, Evalue=1e-79,
Organism=Homo sapiens, GI93277076, Length=449, Percent_Identity=29.6213808463252, Blast_Score=174, Evalue=1e-43,
Organism=Escherichia coli, GI1786882, Length=473, Percent_Identity=81.183932346723, Blast_Score=819, Evalue=0.0,
Organism=Escherichia coli, GI1787057, Length=431, Percent_Identity=29.0023201856148, Blast_Score=162, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17553146, Length=461, Percent_Identity=34.4902386117137, Blast_Score=259, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI71996771, Length=339, Percent_Identity=28.023598820059, Blast_Score=121, Evalue=6e-28,
Organism=Drosophila melanogaster, GI21356207, Length=475, Percent_Identity=35.7894736842105, Blast_Score=286, Evalue=2e-77,
Organism=Drosophila melanogaster, GI19922432, Length=456, Percent_Identity=29.8245614035088, Blast_Score=166, Evalue=5e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 53554; Mature: 53423

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQKLHIKTWGCQMNEYDSSKMADLLNSTHGLELTEIPEEADVLLLNTCSIREKAQEKVF
CCCEEEEEEECCCCCCCCHHHHHHHHHCCCCCEEECCCCCCCEEEEECHHHHHHHHHHHH
HQLGRWKELKKHKPGLVIGVGGCVASQEGEHIRTRAPYVDIIFGPQTLHRLPEMINQIRG
HHHHHHHHHHHCCCCEEEECCCEEECCCCCCEECCCCEEEEEECHHHHHHHHHHHHHHCC
GKSSVVDVSFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCSFCVVPYTRGEEVSRPVDD
CCCCEEECCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCHHCCCHHH
VLFEIAQLAEQGVREVNLLGQNVNAYRGATHDDGICTFAELLRLVAAIDGIDRLRFTTSH
HHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHEECCCC
PIEFTDDIIDVYRDTPELVSFLHLPVQSGSDRVLSMMKRNHTALEYKSIIRKLRAVRPEI
CCCHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCE
QISSDFIVGFPGETAEDFEQTMNLIAQVNFDMSFSFIYSARPGTPAADMPDDVTEEEKKQ
EECCCEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHH
RLYVLQQRINNQAAQFSRAMLGTEQRVLVEGPSKKDLMELTGRTETNRIVNFVGTPDMIG
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHCCCCCCHHEEECCCHHHHC
KFVDIKITDVFTNSLRGEVVRTEEQMGLRVVQSPQMVINRTRKEDELGVGRYHA
CEEEEEEHHHHHHHHCCCEEECHHHCCCEEECCCHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TQKLHIKTWGCQMNEYDSSKMADLLNSTHGLELTEIPEEADVLLLNTCSIREKAQEKVF
CCEEEEEEECCCCCCCCHHHHHHHHHCCCCCEEECCCCCCCEEEEECHHHHHHHHHHHH
HQLGRWKELKKHKPGLVIGVGGCVASQEGEHIRTRAPYVDIIFGPQTLHRLPEMINQIRG
HHHHHHHHHHHCCCCEEEECCCEEECCCCCCEECCCCEEEEEECHHHHHHHHHHHHHHCC
GKSSVVDVSFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCSFCVVPYTRGEEVSRPVDD
CCCCEEECCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCHHCCCHHH
VLFEIAQLAEQGVREVNLLGQNVNAYRGATHDDGICTFAELLRLVAAIDGIDRLRFTTSH
HHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHEECCCC
PIEFTDDIIDVYRDTPELVSFLHLPVQSGSDRVLSMMKRNHTALEYKSIIRKLRAVRPEI
CCCHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCE
QISSDFIVGFPGETAEDFEQTMNLIAQVNFDMSFSFIYSARPGTPAADMPDDVTEEEKKQ
EECCCEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHH
RLYVLQQRINNQAAQFSRAMLGTEQRVLVEGPSKKDLMELTGRTETNRIVNFVGTPDMIG
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHCCCCCCHHEEECCCHHHHC
KFVDIKITDVFTNSLRGEVVRTEEQMGLRVVQSPQMVINRTRKEDELGVGRYHA
CEEEEEEHHHHHHHHCCCEEECHHHCCCEEECCCHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA