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The map label for this gene is tolB

Identifier: 15602832

GI number: 15602832

Start: 1139455

End: 1140753

Strand: Reverse

Name: tolB

Synonym: PM0967

Alternate gene names: 15602832

Gene position: 1140753-1139455 (Counterclockwise)

Preceding gene: 15602833

Following gene: 15602831

Centisome position: 50.53

GC content: 42.49

Gene sequence:

>1299_bases
ATGAAATTAGTCACCCGTATGTGGAGTATATTGATCGTATTCTTTCTTGCTGTATTGCAACCTGCACAAGCAGAGGTACG
AATTGTCATTGATGAAGGTGTAGATAGTGCCAGACCAATTGCGGTTGTGCCATTTAAGTGGAATGGACCGGGTTCTGCAC
CAGCGGATATTGCCGATATCATTGCTGCCGATTTACGCCATAGTGGTAAATTTAATCCGATTCCAGTCAATCGGATGCCA
CAGCACCCAACATCTGTTTCAGAAGTGAATCCAGAAGCTTGGGCTGCGCTTGGTATTGATGCGGTAGTAGTGGGGCATGT
AACACCAGCAAATGGCAGTTTTAACATCGCTTATCAGCTTGTTGATACGATTGGTGCAACTGGTACTGCGGGTACGGTGT
TAACACAAAACCAATATACAGTGACAGCAAAATGGTTGCGTTATGGAGCACATACAGTCAGTGACGAAGTTTTTGAAAAA
TTAACGGGCATCAGAGGGGCGTTTAGAACACGCATTGCTTATATTGTGCAGAAACACGGTGGCTCTCAACCTTATGAAGT
CAGAGTATCTGATTACGATGGGTTTAATCAATTTGTCGTTAACCGTAGTGCGCAACCTTTAATGTCACCAGCTTGGTCAC
ATGATGGTAAAAAATTAGCTTATGTTTCTTTTGAAAATAAAAAATCCCAGCTAGTAGTGCAGGATTTAGGCTCTGGTGCA
CGTAAAGTGGTCGCGTCGTTTAATGGACATAATGGTGCGCCTGCATTTTCACCAGACGGTTCAAGATTAGCCTTTGCCTC
TTCAAAAGACGGTGTATTGAATATTTATGTAATGAATTTAGGTAGTGGACAGATTTCACAGTTGACTAGTGGTACAGGGA
ATAATACTGAACCATCTTGGTCACCTGATGGACAAACAATTGTGTTCACATCTGATAGAAGCGGTTCACCGCAAGTCTAC
CAAATGAGTGCATCCGGTGGTGGTGCATCATTAGTAAGCACTGGTGGTCGCAGTTATAGTGGGCAAATGACTGCCGATGG
TAGCGCTATAATCATGATCAGTAGAGATAATATTGTGAAAAAGGATTTAGCTTCTGGCTCCACAGAAGTGCTAAGTTCCA
CTTTCTTAGATGAAAATCCAAGTATTTCCCCTAACGGTATCATGATCATTTACAGCTCTACTCAAGGGTTAGGAAAAGTG
CTACAATTGGTATCCGCTGACGGTCGTTTTAAAGCACGGTTACCAGGAAATGATGGACAAGTTAAATTCCCAGCTTGGTC
ACCTTATTTAACTAAATAA

Upstream 100 bases:

>100_bases
TCCCCAATTGAATTTAAATTAAGATAAAGTTAGTATTGCCTTTTTGTTTATCGTAAATCAACGATAAAGGATTGGAAATG
AAAAATAATAGGTGGTTCCA

Downstream 100 bases:

>100_bases
AAAACTCTAAGGAGAAAACAATGAAAAAACTAACTAAAGTATTATTAGTTGCTGGTTCTGTTGCTGTATTAGCTGCTTGT
GGTTCATCTAAAAAAGATGA

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MKLVTRMWSILIVFFLAVLQPAQAEVRIVIDEGVDSARPIAVVPFKWNGPGSAPADIADIIAADLRHSGKFNPIPVNRMP
QHPTSVSEVNPEAWAALGIDAVVVGHVTPANGSFNIAYQLVDTIGATGTAGTVLTQNQYTVTAKWLRYGAHTVSDEVFEK
LTGIRGAFRTRIAYIVQKHGGSQPYEVRVSDYDGFNQFVVNRSAQPLMSPAWSHDGKKLAYVSFENKKSQLVVQDLGSGA
RKVVASFNGHNGAPAFSPDGSRLAFASSKDGVLNIYVMNLGSGQISQLTSGTGNNTEPSWSPDGQTIVFTSDRSGSPQVY
QMSASGGGASLVSTGGRSYSGQMTADGSAIIMISRDNIVKKDLASGSTEVLSSTFLDENPSISPNGIMIIYSSTQGLGKV
LQLVSADGRFKARLPGNDGQVKFPAWSPYLTK

Sequences:

>Translated_432_residues
MKLVTRMWSILIVFFLAVLQPAQAEVRIVIDEGVDSARPIAVVPFKWNGPGSAPADIADIIAADLRHSGKFNPIPVNRMP
QHPTSVSEVNPEAWAALGIDAVVVGHVTPANGSFNIAYQLVDTIGATGTAGTVLTQNQYTVTAKWLRYGAHTVSDEVFEK
LTGIRGAFRTRIAYIVQKHGGSQPYEVRVSDYDGFNQFVVNRSAQPLMSPAWSHDGKKLAYVSFENKKSQLVVQDLGSGA
RKVVASFNGHNGAPAFSPDGSRLAFASSKDGVLNIYVMNLGSGQISQLTSGTGNNTEPSWSPDGQTIVFTSDRSGSPQVY
QMSASGGGASLVSTGGRSYSGQMTADGSAIIMISRDNIVKKDLASGSTEVLSSTFLDENPSISPNGIMIIYSSTQGLGKV
LQLVSADGRFKARLPGNDGQVKFPAWSPYLTK
>Mature_432_residues
MKLVTRMWSILIVFFLAVLQPAQAEVRIVIDEGVDSARPIAVVPFKWNGPGSAPADIADIIAADLRHSGKFNPIPVNRMP
QHPTSVSEVNPEAWAALGIDAVVVGHVTPANGSFNIAYQLVDTIGATGTAGTVLTQNQYTVTAKWLRYGAHTVSDEVFEK
LTGIRGAFRTRIAYIVQKHGGSQPYEVRVSDYDGFNQFVVNRSAQPLMSPAWSHDGKKLAYVSFENKKSQLVVQDLGSGA
RKVVASFNGHNGAPAFSPDGSRLAFASSKDGVLNIYVMNLGSGQISQLTSGTGNNTEPSWSPDGQTIVFTSDRSGSPQVY
QMSASGGGASLVSTGGRSYSGQMTADGSAIIMISRDNIVKKDLASGSTEVLSSTFLDENPSISPNGIMIIYSSTQGLGKV
LQLVSADGRFKARLPGNDGQVKFPAWSPYLTK

Specific function: Involved in the tonB-independent uptake of proteins

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family

Homologues:

Organism=Escherichia coli, GI1786961, Length=436, Percent_Identity=63.0733944954128, Blast_Score=552, Evalue=1e-158,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOLB_PASMU (Q9CM71)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245904.1
- ProteinModelPortal:   Q9CM71
- SMR:   Q9CM71
- GeneID:   1244314
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0967
- NMPDR:   fig|272843.1.peg.967
- HOGENOM:   HBG644919
- OMA:   RVADYDG
- ProtClustDB:   PRK01742
- BioCyc:   PMUL272843:PM0967-MONOMER
- HAMAP:   MF_00671
- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR001680
- Gene3D:   G3DSA:3.40.50.10070
- SMART:   SM00320
- TIGRFAMs:   TIGR02800

Pfam domain/function: PF07676 PD40; PF04052 TolB_N; SSF52964 TolB_N

EC number: NA

Molecular weight: Translated: 46004; Mature: 46004

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLVTRMWSILIVFFLAVLQPAQAEVRIVIDEGVDSARPIAVVPFKWNGPGSAPADIADI
CHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEEEEEECCCCCCCHHHHHH
IAADLRHSGKFNPIPVNRMPQHPTSVSEVNPEAWAALGIDAVVVGHVTPANGSFNIAYQL
HHHHHHHCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHCCCEEEEEEEECCCCCEEEEEEE
VDTIGATGTAGTVLTQNQYTVTAKWLRYGAHTVSDEVFEKLTGIRGAFRTRIAYIVQKHG
EHHHCCCCCCCEEEECCCEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GSQPYEVRVSDYDGFNQFVVNRSAQPLMSPAWSHDGKKLAYVSFENKKSQLVVQDLGSGA
CCCCEEEEECCCCCCHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEHHHHCCCH
RKVVASFNGHNGAPAFSPDGSRLAFASSKDGVLNIYVMNLGSGQISQLTSGTGNNTEPSW
HHHHEECCCCCCCCCCCCCCCEEEEECCCCCEEEEEEEEECCCCHHHHHCCCCCCCCCCC
SPDGQTIVFTSDRSGSPQVYQMSASGGGASLVSTGGRSYSGQMTADGSAIIMISRDNIVK
CCCCCEEEEECCCCCCCCEEEEECCCCCCEEEECCCCCCCCEEECCCCEEEEEECCCHHH
KDLASGSTEVLSSTFLDENPSISPNGIMIIYSSTQGLGKVLQLVSADGRFKARLPGNDGQ
HHHCCCCHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCCCEEEECCCCCCE
VKFPAWSPYLTK
EECCCCCCCCCC
>Mature Secondary Structure
MKLVTRMWSILIVFFLAVLQPAQAEVRIVIDEGVDSARPIAVVPFKWNGPGSAPADIADI
CHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEEEEEECCCCCCCHHHHHH
IAADLRHSGKFNPIPVNRMPQHPTSVSEVNPEAWAALGIDAVVVGHVTPANGSFNIAYQL
HHHHHHHCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHCCCEEEEEEEECCCCCEEEEEEE
VDTIGATGTAGTVLTQNQYTVTAKWLRYGAHTVSDEVFEKLTGIRGAFRTRIAYIVQKHG
EHHHCCCCCCCEEEECCCEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GSQPYEVRVSDYDGFNQFVVNRSAQPLMSPAWSHDGKKLAYVSFENKKSQLVVQDLGSGA
CCCCEEEEECCCCCCHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEHHHHCCCH
RKVVASFNGHNGAPAFSPDGSRLAFASSKDGVLNIYVMNLGSGQISQLTSGTGNNTEPSW
HHHHEECCCCCCCCCCCCCCCEEEEECCCCCEEEEEEEEECCCCHHHHHCCCCCCCCCCC
SPDGQTIVFTSDRSGSPQVYQMSASGGGASLVSTGGRSYSGQMTADGSAIIMISRDNIVK
CCCCCEEEEECCCCCCCCEEEEECCCCCCEEEECCCCCCCCEEECCCCEEEEEECCCHHH
KDLASGSTEVLSSTFLDENPSISPNGIMIIYSSTQGLGKVLQLVSADGRFKARLPGNDGQ
HHHCCCCHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCCCEEEECCCCCCE
VKFPAWSPYLTK
EECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100