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The map label for this gene is purU [H]

Identifier: 15602738

GI number: 15602738

Start: 1027248

End: 1028084

Strand: Direct

Name: purU [H]

Synonym: PM0873

Alternate gene names: 15602738

Gene position: 1027248-1028084 (Clockwise)

Preceding gene: 15602737

Following gene: 15602741

Centisome position: 45.5

GC content: 34.65

Gene sequence:

>837_bases
ATGATTGAAAATAAAATATTATTAACCGATTGTCCAGATGATAAAGGTTTAATCGCAAAAATTACCAACATTTGTTACAA
ACATCAGCTCAATATCATTCATAACAATGAATTTGTGGATTTTGACACAAAGCATTTCTTTATGCGCACTGAACTACAGG
GCATTTTTAATGAAGATACCTTATTAGCTGACTTAAAATTTAGTCTTCCAGAGGGAACAAATTGTCGCTTAATTAGTGCG
AAAAGAAAACGCATTGTCATTTTAGTCACCAAAGAAGCGCATTGCCTTGGCGATATTTTAATGAAAAATTACTATGGTGG
TTTAGACGTAGAAATCGCTGCGGTGATTGGTAATCATGATACGTTAAGAACATTAGTAGAACGCTTTGACATCCCATTCC
ACTACATTAGCCATCATGATCTCACACGAGTAGAACATGATAAGTTACTTGCAGATAAAATCGATGAATATACACCAGAT
TATATTGTGTTAGCAAAATACATGCGGGTACTCAACCCTCAGTTTGTTGAAAAATATCCAAATCGAGTGATCAATATTCA
TCATTCTTTTTTACCTGCATTTATTGGTGCAAAACCTTATCACCAAGCTTATGAACGTGGTGTCAAAATTATTGGCGCAA
CTGCACACTTTATTAATAACGAATTAGATCAAGGTCCTATTATTATGCAAAATGTGATCAACGTGGATCACACTTATAAT
GCAGAAGCTATGATGCGTGCAGGACGAGATGTTGAAAAAACAGTATTAAGCCGTGCATTAGATCTGGCTTTGCACGATCG
TATTTTTGTTTACAAAAATAAGACGGTTGTTCTCTAA

Upstream 100 bases:

>100_bases
ATCCCTGAGTTCATTCGAAATTTAATTTATTTTACTTTGTCGTAAAGCCCATTTCTTGATGGGTTTTCTTTTTTATTTTT
TCGACTAAAACAACACACTT

Downstream 100 bases:

>100_bases
AAAGCACATCAAAGCAATGTAATACAAAACCACTTTTATCCGGCGTGGTTTTGATTTAGCGATAAAAAAATAGCGTGTTA
ATTTAACACGCTATTTTTAA

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MIENKILLTDCPDDKGLIAKITNICYKHQLNIIHNNEFVDFDTKHFFMRTELQGIFNEDTLLADLKFSLPEGTNCRLISA
KRKRIVILVTKEAHCLGDILMKNYYGGLDVEIAAVIGNHDTLRTLVERFDIPFHYISHHDLTRVEHDKLLADKIDEYTPD
YIVLAKYMRVLNPQFVEKYPNRVINIHHSFLPAFIGAKPYHQAYERGVKIIGATAHFINNELDQGPIIMQNVINVDHTYN
AEAMMRAGRDVEKTVLSRALDLALHDRIFVYKNKTVVL

Sequences:

>Translated_278_residues
MIENKILLTDCPDDKGLIAKITNICYKHQLNIIHNNEFVDFDTKHFFMRTELQGIFNEDTLLADLKFSLPEGTNCRLISA
KRKRIVILVTKEAHCLGDILMKNYYGGLDVEIAAVIGNHDTLRTLVERFDIPFHYISHHDLTRVEHDKLLADKIDEYTPD
YIVLAKYMRVLNPQFVEKYPNRVINIHHSFLPAFIGAKPYHQAYERGVKIIGATAHFINNELDQGPIIMQNVINVDHTYN
AEAMMRAGRDVEKTVLSRALDLALHDRIFVYKNKTVVL
>Mature_278_residues
MIENKILLTDCPDDKGLIAKITNICYKHQLNIIHNNEFVDFDTKHFFMRTELQGIFNEDTLLADLKFSLPEGTNCRLISA
KRKRIVILVTKEAHCLGDILMKNYYGGLDVEIAAVIGNHDTLRTLVERFDIPFHYISHHDLTRVEHDKLLADKIDEYTPD
YIVLAKYMRVLNPQFVEKYPNRVINIHHSFLPAFIGAKPYHQAYERGVKIIGATAHFINNELDQGPIIMQNVINVDHTYN
AEAMMRAGRDVEKTVLSRALDLALHDRIFVYKNKTVVL

Specific function: Produces formate from formyl-tetrahydrofolate. Provides the major source of formate for the purT-dependent synthesis of 5'-phosphoribosyl-N-formylglycinamide (FGAR) during aerobic growth. Has a role in regulating the one-carbon pool [H]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=193, Percent_Identity=30.0518134715026, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI209869995, Length=193, Percent_Identity=30.0518134715026, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI209869993, Length=193, Percent_Identity=30.0518134715026, Blast_Score=96, Evalue=5e-20,
Organism=Escherichia coli, GI1787483, Length=277, Percent_Identity=73.2851985559567, Blast_Score=429, Evalue=1e-121,
Organism=Escherichia coli, GI1788846, Length=94, Percent_Identity=43.6170212765958, Blast_Score=89, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17567511, Length=168, Percent_Identity=31.547619047619, Blast_Score=100, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24582400, Length=192, Percent_Identity=29.1666666666667, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32087; Mature: 32087

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIENKILLTDCPDDKGLIAKITNICYKHQLNIIHNNEFVDFDTKHFFMRTELQGIFNEDT
CCCCCEEEEECCCCCCHHHHHHHHHHHHEEEEEECCEEEEECCHHHEEHHHHHHCCCCCE
LLADLKFSLPEGTNCRLISAKRKRIVILVTKEAHCLGDILMKNYYGGLDVEIAAVIGNHD
EEEEEEECCCCCCCEEEEECCCCEEEEEEECCHHHHHHHHHHHCCCCCCEEEEEEECCCH
TLRTLVERFDIPFHYISHHDLTRVEHDKLLADKIDEYTPDYIVLAKYMRVLNPQFVEKYP
HHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHCC
NRVINIHHSFLPAFIGAKPYHQAYERGVKIIGATAHFINNELDQGPIIMQNVINVDHTYN
CCEEEEHHHHHHHHHCCCCHHHHHHCCCEEEEHHHHHHCCCCCCCCCHHHHHHCCCCCCC
AEAMMRAGRDVEKTVLSRALDLALHDRIFVYKNKTVVL
HHHHHHCCCHHHHHHHHHHHHHHHHCEEEEEECCEEEC
>Mature Secondary Structure
MIENKILLTDCPDDKGLIAKITNICYKHQLNIIHNNEFVDFDTKHFFMRTELQGIFNEDT
CCCCCEEEEECCCCCCHHHHHHHHHHHHEEEEEECCEEEEECCHHHEEHHHHHHCCCCCE
LLADLKFSLPEGTNCRLISAKRKRIVILVTKEAHCLGDILMKNYYGGLDVEIAAVIGNHD
EEEEEEECCCCCCCEEEEECCCCEEEEEEECCHHHHHHHHHHHCCCCCCEEEEEEECCCH
TLRTLVERFDIPFHYISHHDLTRVEHDKLLADKIDEYTPDYIVLAKYMRVLNPQFVEKYP
HHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHCC
NRVINIHHSFLPAFIGAKPYHQAYERGVKIIGATAHFINNELDQGPIIMQNVINVDHTYN
CCEEEEHHHHHHHHHCCCCHHHHHHCCCEEEEHHHHHHCCCCCCCCCHHHHHHCCCCCCC
AEAMMRAGRDVEKTVLSRALDLALHDRIFVYKNKTVVL
HHHHHHCCCHHHHHHHHHHHHHHHHCEEEEEECCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 8335255 [H]