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The map label for this gene is manX [H]

Identifier: 15602699

GI number: 15602699

Start: 983514

End: 984488

Strand: Reverse

Name: manX [H]

Synonym: PM0834

Alternate gene names: 15602699

Gene position: 984488-983514 (Counterclockwise)

Preceding gene: 15602701

Following gene: 15602698

Centisome position: 43.61

GC content: 42.77

Gene sequence:

>975_bases
ATGACAATTGCTATTATGATAGCGACTCATGGGGTAGCAGCAGAGCAATTGCTGAAAACTACGGAAATGTTGATTGGTGA
GCAAGACAATGTTGCCACTATCGATTTTGTTCCGGGAGAAAATGCTGAAACTATCATGACCAAATACCAAGAAAAACTAT
CAACCACTTTATCCCATTGTGATGAGGTGTTGTTTTTAGTGGATACTTGGGGTGGCAGTCCATTTAATGCGGCAAATCGC
GTTGCTGATGGTAAGCCAAATATGGACATCGTGACTGGGGTAAATGTCCCGATGTTAGTGGAAACCTTTATGGCACGTGA
TGATGGACCAACATTACCTGAATTGGTAGCGATTGCGCTTGAAACGGGTCGCACCGGTGTGCGTGCATTACGCTATGAGG
AAGAGGAAGTCGCAACACCAGTCCAAAATGTGGAAGAAAAACCCGTTGAACCGACAGTTGTTACCGCAAATGGTGTTGGG
CATTTAGAAATTGGTTTGGCACGTATTGATGACCGTTTAATTCATGGTCAAGTCGCAACCCGTTGGACGAAAGAAAGTAA
AGTGAGCCGTATTGTTGTGGTCAATGATGATGTGGCGAAGGACAGCGTACGTTCAACCATGTTGAAAAGTGTTGCACCGC
CGGGCGTAACTGCACATGTCGTGGATGTAGAAAAAATGATCCGTGTATATAACAACCCAGAATATGCGGGCGAGCGTATG
ATGTTATTGTTTACTAATCCGACCGATGTTGTCCGTTTAATGGAAGCAGGTGTGGCGTTTAAATCAATTAATGTTGGTGG
TATGGCGTATAAAGATGGCAAAAAAATGATCACCAGTGCGGTTGCTGTCGATGATAAAGATATTGAGGCATTTAAGAAAC
TGGATGCACAAAACATTGAGCTTGATGTGCGTAAAGTCTCAAATGATAGCCGTCAGTATATGATGGATTTATTAAAGAAA
AATAATCTCATCTAA

Upstream 100 bases:

>100_bases
TTAAATTGTGATCTAAATCACATCGTTATTTCATCCTTTACTCTATAATTCGCGCTCAGATAACTCTATTTGATTTTTAA
TTTTTATAAGGAGTCAAACA

Downstream 100 bases:

>100_bases
TGATATTAAGAAAAGGAAATTAGAATGGAAATTTCAACTTTACAAATTGTACTTGTCTTTCTCGTTGCTTGTGTATCAGG
AATGGGATCGATTTTGGATG

Product: hypothetical protein

Products: NA

Alternate protein names: EIIAB-Man; Mannose-specific phosphotransferase enzyme IIA component; EIII-Man; PTS system mannose-specific EIIA component; Mannose-specific phosphotransferase enzyme IIB component; PTS system mannose-specific EIIB component [H]

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MTIAIMIATHGVAAEQLLKTTEMLIGEQDNVATIDFVPGENAETIMTKYQEKLSTTLSHCDEVLFLVDTWGGSPFNAANR
VADGKPNMDIVTGVNVPMLVETFMARDDGPTLPELVAIALETGRTGVRALRYEEEEVATPVQNVEEKPVEPTVVTANGVG
HLEIGLARIDDRLIHGQVATRWTKESKVSRIVVVNDDVAKDSVRSTMLKSVAPPGVTAHVVDVEKMIRVYNNPEYAGERM
MLLFTNPTDVVRLMEAGVAFKSINVGGMAYKDGKKMITSAVAVDDKDIEAFKKLDAQNIELDVRKVSNDSRQYMMDLLKK
NNLI

Sequences:

>Translated_324_residues
MTIAIMIATHGVAAEQLLKTTEMLIGEQDNVATIDFVPGENAETIMTKYQEKLSTTLSHCDEVLFLVDTWGGSPFNAANR
VADGKPNMDIVTGVNVPMLVETFMARDDGPTLPELVAIALETGRTGVRALRYEEEEVATPVQNVEEKPVEPTVVTANGVG
HLEIGLARIDDRLIHGQVATRWTKESKVSRIVVVNDDVAKDSVRSTMLKSVAPPGVTAHVVDVEKMIRVYNNPEYAGERM
MLLFTNPTDVVRLMEAGVAFKSINVGGMAYKDGKKMITSAVAVDDKDIEAFKKLDAQNIELDVRKVSNDSRQYMMDLLKK
NNLI
>Mature_323_residues
TIAIMIATHGVAAEQLLKTTEMLIGEQDNVATIDFVPGENAETIMTKYQEKLSTTLSHCDEVLFLVDTWGGSPFNAANRV
ADGKPNMDIVTGVNVPMLVETFMARDDGPTLPELVAIALETGRTGVRALRYEEEEVATPVQNVEEKPVEPTVVTANGVGH
LEIGLARIDDRLIHGQVATRWTKESKVSRIVVVNDDVAKDSVRSTMLKSVAPPGVTAHVVDVEKMIRVYNNPEYAGERMM
LLFTNPTDVVRLMEAGVAFKSINVGGMAYKDGKKMITSAVAVDDKDIEAFKKLDAQNIELDVRKVSNDSRQYMMDLLKKN
NLI

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG2893

COG function: function code G; Phosphotransferase system, mannose/fructose-specific component IIA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1788120, Length=321, Percent_Identity=64.4859813084112, Blast_Score=404, Evalue=1e-114,
Organism=Escherichia coli, GI87082217, Length=157, Percent_Identity=35.031847133758, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789527, Length=152, Percent_Identity=32.2368421052632, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004701
- InterPro:   IPR013789
- InterPro:   IPR004720
- InterPro:   IPR018455 [H]

Pfam domain/function: PF03610 EIIA-man; PF03830 PTSIIB_sorb [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 35590; Mature: 35459

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS51096 PTS_EIIA_TYPE_4 ; PS51101 PTS_EIIB_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIAIMIATHGVAAEQLLKTTEMLIGEQDNVATIDFVPGENAETIMTKYQEKLSTTLSHC
CEEEEEEEECCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
DEVLFLVDTWGGSPFNAANRVADGKPNMDIVTGVNVPMLVETFMARDDGPTLPELVAIAL
HHEEEEEECCCCCCCCHHHHCCCCCCCCEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHH
ETGRTGVRALRYEEEEVATPVQNVEEKPVEPTVVTANGVGHLEIGLARIDDRLIHGQVAT
HCCCCHHHHHEECHHHHHCCHHHHHHCCCCCEEEEECCCCEEEEEHHHHCCHHHCCHHHH
RWTKESKVSRIVVVNDDVAKDSVRSTMLKSVAPPGVTAHVVDVEKMIRVYNNPEYAGERM
HHCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHCCCCCCCCEE
MLLFTNPTDVVRLMEAGVAFKSINVGGMAYKDGKKMITSAVAVDDKDIEAFKKLDAQNIE
EEEEECHHHHHHHHHHCCEEEEECCCCEEECCCHHHHHHHHHCCCHHHHHHHHCCCCCCE
LDVRKVSNDSRQYMMDLLKKNNLI
EEEEECCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TIAIMIATHGVAAEQLLKTTEMLIGEQDNVATIDFVPGENAETIMTKYQEKLSTTLSHC
EEEEEEEECCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
DEVLFLVDTWGGSPFNAANRVADGKPNMDIVTGVNVPMLVETFMARDDGPTLPELVAIAL
HHEEEEEECCCCCCCCHHHHCCCCCCCCEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHH
ETGRTGVRALRYEEEEVATPVQNVEEKPVEPTVVTANGVGHLEIGLARIDDRLIHGQVAT
HCCCCHHHHHEECHHHHHCCHHHHHHCCCCCEEEEECCCCEEEEEHHHHCCHHHCCHHHH
RWTKESKVSRIVVVNDDVAKDSVRSTMLKSVAPPGVTAHVVDVEKMIRVYNNPEYAGERM
HHCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHCCCCCCCCEE
MLLFTNPTDVVRLMEAGVAFKSINVGGMAYKDGKKMITSAVAVDDKDIEAFKKLDAQNIE
EEEEECHHHHHHHHHHCCEEEEECCCCEEECCCHHHHHHHHHCCCHHHHHHHHCCCCCCE
LDVRKVSNDSRQYMMDLLKKNNLI
EEEEECCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]