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The map label for this gene is prfC [H]

Identifier: 15602681

GI number: 15602681

Start: 965205

End: 966794

Strand: Direct

Name: prfC [H]

Synonym: PM0816

Alternate gene names: 15602681

Gene position: 965205-966794 (Clockwise)

Preceding gene: 15602673

Following gene: 15602682

Centisome position: 42.76

GC content: 42.08

Gene sequence:

>1590_bases
ATGTCATTAAACGATTATCCACAACAAGTAAACAAACGTCGCACCTTTGCGATTATCTCTCACCCCGATGCGGGTAAAAC
CACGATTACCGAAAAAGTTTTGCTCTACGGACAAGCGATTCAAACCGCGGGATCGGTGAAAGGAAAAGGGTCTAGTACTC
ATGCCAAATCCGACTGGATGGAAATGGAAAAACAACGGGGTATTTCGATTACAACATCTGTAATGCAATTCCCTTATAAC
GATTGTCTCGTGAATCTTCTGGATACTCCGGGACACGAAGACTTCTCGGAAGATACCTACCGCACGTTAACCGCTGTTGA
TAGCTGTTTAATGGTGATCGACAGTGCCAAAGGGGTGGAAGAGCGCACGATTAAATTAATGGAAGTTACTCGCTTGCGTG
ATACCCCTATTTTGACTTTTATGAACAAATTGGACCGTGATATTCGTGATCCAATGGAATTGTTAGATGAAGTAGAAAAC
GTATTAAATATCCACTGTGCACCGATCACCTGGCCAATTGGGTGTGGCAAATTATTCAAAGGGGTTTACCACTTATATAA
AGACGAAACCTATTTATACCAAACGGGTCAAGGACATACGATCCAAGAAAAACGTGTGATTAAAGGGTTAGATAACCCAG
AACTTGATGCTGCTGTTGGTGATGATTTAGCTCAACAGTTACGTGATGAATTAGAACTTGTACAAGGTGCATCTAACGAA
TTCGATTTAGACGCATTTTTACAAGGTGAATTAACCCCTGTTTTCTTTGGAACAGCATTAGGTAACTTTGGTGTGGATCA
TTTCTTAGATGGCTTAACCCAATGGGCGCCCGCGCCACAATCACGTCAAGCAGATAGCCGTGCAGTAGCAAGTAGCGAAC
AAAAACTCACCGGTTTCGTGTTTAAAATCCAAGCCAATATGGACCCAAAACACCGTGATCGCGTGGCATTTATGCGCGTT
GTATCTGGCAAATATGAAAAAGGCATGAAACTACGCCATGTAAGACTAGGAAAAGATGTGGTAATTTCCGATGCCTTAAC
TTTTATGGCAGGAGATCGTTCTCATGCTGAAGAGGCTTATGCGGGCGATATTATCGGTTTACACAATCACGGTACGATCC
AAATTGGTGATACCTTCACGCAAGGCGAAGAATTAAAATTCACTGGGATTCCAAACTTTGCACCTGAATTGTTCCGTCGC
ATTCGTTTAAAAGATCCACTCAAACAAAAACAATTATTAAAAGGCTTAGTGCAACTTTCAGAAGAAGGTGCGGTGCAAGT
TTTCCGACCTCTCATGAATAACGATCTCATCGTAGGTGCGGTGGGTGTACTTCAGTTTGATGTGGTGGTTTCACGTTTAA
AATCAGAATATAACGTAGAAGCGATTTATGAAAACATCAACGTGGCGACGGCACGCTGGGTTGAATGTAGCGATGCGAAG
AAATTCGACGAATTTAAGCGTAAAAATGAGCAAAACCTAGCGTTAGATGGTGGCGATAATCTGACTTATATTGCGCCAAC
CATGGTGAACTTAAATCTGGCACAAGAACGTTATCCTGACGTCAAATTCTTTAAAACCAGAGAACATTAA

Upstream 100 bases:

>100_bases
GTTTGCATTTTTTGTTGCATCTATAAATAAATTTGAGAAGAACATCAAAATAGGCGAAAATAGCCTATTATTTTTAACGA
AAATACATTGATAACAAAAC

Downstream 100 bases:

>100_bases
TAAATAAAAGTGCGGTTGAATGTTGAGACCGCACTTTTTGCACATAATAACCTTTCAAAAATCAGGTATTCATTTATATT
ACCTATCAAATTTATTATCA

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MSLNDYPQQVNKRRTFAIISHPDAGKTTITEKVLLYGQAIQTAGSVKGKGSSTHAKSDWMEMEKQRGISITTSVMQFPYN
DCLVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVEERTIKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
VLNIHCAPITWPIGCGKLFKGVYHLYKDETYLYQTGQGHTIQEKRVIKGLDNPELDAAVGDDLAQQLRDELELVQGASNE
FDLDAFLQGELTPVFFGTALGNFGVDHFLDGLTQWAPAPQSRQADSRAVASSEQKLTGFVFKIQANMDPKHRDRVAFMRV
VSGKYEKGMKLRHVRLGKDVVISDALTFMAGDRSHAEEAYAGDIIGLHNHGTIQIGDTFTQGEELKFTGIPNFAPELFRR
IRLKDPLKQKQLLKGLVQLSEEGAVQVFRPLMNNDLIVGAVGVLQFDVVVSRLKSEYNVEAIYENINVATARWVECSDAK
KFDEFKRKNEQNLALDGGDNLTYIAPTMVNLNLAQERYPDVKFFKTREH

Sequences:

>Translated_529_residues
MSLNDYPQQVNKRRTFAIISHPDAGKTTITEKVLLYGQAIQTAGSVKGKGSSTHAKSDWMEMEKQRGISITTSVMQFPYN
DCLVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVEERTIKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
VLNIHCAPITWPIGCGKLFKGVYHLYKDETYLYQTGQGHTIQEKRVIKGLDNPELDAAVGDDLAQQLRDELELVQGASNE
FDLDAFLQGELTPVFFGTALGNFGVDHFLDGLTQWAPAPQSRQADSRAVASSEQKLTGFVFKIQANMDPKHRDRVAFMRV
VSGKYEKGMKLRHVRLGKDVVISDALTFMAGDRSHAEEAYAGDIIGLHNHGTIQIGDTFTQGEELKFTGIPNFAPELFRR
IRLKDPLKQKQLLKGLVQLSEEGAVQVFRPLMNNDLIVGAVGVLQFDVVVSRLKSEYNVEAIYENINVATARWVECSDAK
KFDEFKRKNEQNLALDGGDNLTYIAPTMVNLNLAQERYPDVKFFKTREH
>Mature_528_residues
SLNDYPQQVNKRRTFAIISHPDAGKTTITEKVLLYGQAIQTAGSVKGKGSSTHAKSDWMEMEKQRGISITTSVMQFPYND
CLVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVEERTIKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVENV
LNIHCAPITWPIGCGKLFKGVYHLYKDETYLYQTGQGHTIQEKRVIKGLDNPELDAAVGDDLAQQLRDELELVQGASNEF
DLDAFLQGELTPVFFGTALGNFGVDHFLDGLTQWAPAPQSRQADSRAVASSEQKLTGFVFKIQANMDPKHRDRVAFMRVV
SGKYEKGMKLRHVRLGKDVVISDALTFMAGDRSHAEEAYAGDIIGLHNHGTIQIGDTFTQGEELKFTGIPNFAPELFRRI
RLKDPLKQKQLLKGLVQLSEEGAVQVFRPLMNNDLIVGAVGVLQFDVVVSRLKSEYNVEAIYENINVATARWVECSDAKK
FDEFKRKNEQNLALDGGDNLTYIAPTMVNLNLAQERYPDVKFFKTREH

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=491, Percent_Identity=27.4949083503055, Blast_Score=166, Evalue=6e-41,
Organism=Homo sapiens, GI19923640, Length=510, Percent_Identity=25.8823529411765, Blast_Score=136, Evalue=6e-32,
Organism=Homo sapiens, GI25306283, Length=394, Percent_Identity=27.9187817258883, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI25306287, Length=482, Percent_Identity=25.5186721991701, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI157426893, Length=182, Percent_Identity=28.021978021978, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI94966754, Length=157, Percent_Identity=28.0254777070064, Blast_Score=73, Evalue=7e-13,
Organism=Escherichia coli, GI1790835, Length=529, Percent_Identity=86.7674858223062, Blast_Score=971, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=498, Percent_Identity=25.9036144578313, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI1788922, Length=156, Percent_Identity=30.7692307692308, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI48994988, Length=199, Percent_Identity=29.6482412060301, Blast_Score=87, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17533571, Length=517, Percent_Identity=26.8858800773694, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17556745, Length=163, Percent_Identity=31.9018404907975, Blast_Score=98, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17557151, Length=135, Percent_Identity=29.6296296296296, Blast_Score=76, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17506493, Length=162, Percent_Identity=32.0987654320988, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323098, Length=482, Percent_Identity=25.9336099585062, Blast_Score=171, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6322359, Length=420, Percent_Identity=25.952380952381, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6323320, Length=153, Percent_Identity=24.8366013071895, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6324707, Length=155, Percent_Identity=28.3870967741935, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6320593, Length=155, Percent_Identity=28.3870967741935, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24582462, Length=478, Percent_Identity=27.4058577405858, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI221458488, Length=522, Percent_Identity=24.1379310344828, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI78706572, Length=159, Percent_Identity=28.9308176100629, Blast_Score=83, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 59517; Mature: 59386

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLNDYPQQVNKRRTFAIISHPDAGKTTITEKVLLYGQAIQTAGSVKGKGSSTHAKSDWM
CCCCCCHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
EMEKQRGISITTSVMQFPYNDCLVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVE
HHHHHCCCEEEHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCH
ERTIKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVENVLNIHCAPITWPIGCGKLFK
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCEECCCCHHHHHH
GVYHLYKDETYLYQTGQGHTIQEKRVIKGLDNPELDAAVGDDLAQQLRDELELVQGASNE
HHHHHHCCCEEEEECCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
FDLDAFLQGELTPVFFGTALGNFGVDHFLDGLTQWAPAPQSRQADSRAVASSEQKLTGFV
CCCHHHHCCCCCCEEEHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCEEEE
FKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRHVRLGKDVVISDALTFMAGDRSHAEEAY
EEEEECCCCCHHHHHHHHHHHHCHHHCCCEEEEEECCCCCHHHHHHHHHCCCHHHHHHHH
AGDIIGLHNHGTIQIGDTFTQGEELKFTGIPNFAPELFRRIRLKDPLKQKQLLKGLVQLS
CCCEEEECCCCEEEECCCCCCCCCEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHC
EEGAVQVFRPLMNNDLIVGAVGVLQFDVVVSRLKSEYNVEAIYENINVATARWVECSDAK
CCCHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHCCHHHHHHCCCEEEEEEEECCCHH
KFDEFKRKNEQNLALDGGDNLTYIAPTMVNLNLAQERYPDVKFFKTREH
HHHHHHHCCCCCEEEECCCCEEEEECEEEEEEEHHHHCCCCEEEECCCC
>Mature Secondary Structure 
SLNDYPQQVNKRRTFAIISHPDAGKTTITEKVLLYGQAIQTAGSVKGKGSSTHAKSDWM
CCCCCHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
EMEKQRGISITTSVMQFPYNDCLVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVE
HHHHHCCCEEEHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCH
ERTIKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVENVLNIHCAPITWPIGCGKLFK
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCEECCCCHHHHHH
GVYHLYKDETYLYQTGQGHTIQEKRVIKGLDNPELDAAVGDDLAQQLRDELELVQGASNE
HHHHHHCCCEEEEECCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
FDLDAFLQGELTPVFFGTALGNFGVDHFLDGLTQWAPAPQSRQADSRAVASSEQKLTGFV
CCCHHHHCCCCCCEEEHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCEEEE
FKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRHVRLGKDVVISDALTFMAGDRSHAEEAY
EEEEECCCCCHHHHHHHHHHHHCHHHCCCEEEEEECCCCCHHHHHHHHHCCCHHHHHHHH
AGDIIGLHNHGTIQIGDTFTQGEELKFTGIPNFAPELFRRIRLKDPLKQKQLLKGLVQLS
CCCEEEECCCCEEEECCCCCCCCCEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHC
EEGAVQVFRPLMNNDLIVGAVGVLQFDVVVSRLKSEYNVEAIYENINVATARWVECSDAK
CCCHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHCCHHHHHHCCCEEEEEEEECCCHH
KFDEFKRKNEQNLALDGGDNLTYIAPTMVNLNLAQERYPDVKFFKTREH
HHHHHHHCCCCCEEEECCCCEEEEECEEEEEEEHHHHCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA