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The map label for this gene is nusA [H]

Identifier: 15602625

GI number: 15602625

Start: 895990

End: 897474

Strand: Reverse

Name: nusA [H]

Synonym: PM0760

Alternate gene names: 15602625

Gene position: 897474-895990 (Counterclockwise)

Preceding gene: 15602626

Following gene: 15602624

Centisome position: 39.76

GC content: 41.41

Gene sequence:

>1485_bases
ATGAGTAAAGAACTTTTATTGGCTGCAGAAGCGGTATCAAATGAAAAATTATTACCACGTGAAAAGATTTTTGAAGCGTT
AGAAAGTGCGCTTGCCCTTTCAACCAAAAAGAAGTATGAGCAAGAAATTGATGTGCGTGTGGTGATTAACACAAAAACGG
GGGAGTTTGATACTTTCCGTCGTTGGTTGGTTGTGGACGAAGTAACTAATCCGACAAAAGAAATCACCTTAGAAGCGGCA
CAATTTGACGATCCTAATGTGCAACTAGGTGATTATGTAGAAGATCAAATTGAATCTGTGGCCTTTGACCGCATCACGAT
GCAAACTGCACGTCAGGTGATTAGCACGAAAATCCGCGAAGCAGAGCGTAACAAAATTGTTGAGCAATTCCGTTCTCAAG
AAGGCGAGATTGTGACCGGCACAGTGAAAAAAGTGAATCGCGACAATATTGTATTAGATTTAGGTCAACAAGCCGAAGCC
GTGATATTACGTGAAGATATGTTACCGCGTGAAAATTTCCGTCCGGGAGATCGTGTCCGTGGTGTGTTATATAAAGTGAG
CCCAGAAAGCAAAGGTGCTCAATTATTTGTTACCCGTGCTAAACCGGAAATGTTAATTGAGTTATTCCGTATTGAGGTTC
CTGAAATTGGTGAAGAACTGATCGAAATTAAAAATGCTGCGCGTGATCCGGGTTCTCGAGCGAAAATTGCAGTGAAAAGC
AATGATAAACGTATTGACCCAGTGGGTGCTTGTGTTGGTATGCGTGGTGCGCGTGTACAAGCGATTACCAATGAATTAGG
TGGTGAACGTGTGGATATCGTACTTTGGGATGATAACCCTGCGCAATTTGTGATCAATGCGATGGCACCAGCAGATGTTA
CTTCTATCGTGGTGGATGAAGATTCACATGCAATGGATATCGCAGTCGAACCAGCCAATCTTGCACAAGCGATTGGTCGA
AATGGTCAAAATGTGCGTTTAGCAACCCAACTAACAAGTTGGACATTAAACGTGATGACCACTGATGAGCTGAACCAAAA
ACATCAAGCAGAAGATAATAAAGTATTGAAATTATTCATGAATGCGTTAGAAATTGATGAAGATTTTGCGCATATCTTAA
TTGAGGAAGGTTTCTCTACATTAGAAGAACTGGCTTATGTTTCAGTCAGTGAATTAACCGCAATTGATGGCTTAGAAGAT
GAAGATTTGGTCGAAGAATTGCAAGCGCGTGCCAAAAATGCGATTACGGCTGCGGCATTAGCAGAAGAAGAAGCCTTAAA
ACAAGCGCATATTGAAGATCGTTTACTCGCATTAGAGGGAATGAATCGCCATATTGCGTTGAAATTAGCAGAAAAACAAA
TTACTACTCTGGAAGAACTTGCAGAACAAGGTGTTGATGATTTAGCGGATATTGAAGATTTGACAGCAGAACAAGCCGCC
GATTTTATTATGGCGGCACGTAATATCTGTTGGTTCAGTGAGTAA

Upstream 100 bases:

>100_bases
TGTTGACCCTGATTGTCGATGGGCAAGAACAAATTTTGGTATTTGGCAATATTCAAAAGGCCAATATTATTCCTAAATTC
TAAAAGGAGCAAACAGAGCA

Downstream 100 bases:

>100_bases
TTGGAGGAGTGAAAAATGACAGAAGATGTAAAAAAAGCCGATGGGGCTGCCCCAAAAAAACTGAGTTTACAGCGTCGAAC
TAAAACCACCGTCAGTACAA

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MSKELLLAAEAVSNEKLLPREKIFEALESALALSTKKKYEQEIDVRVVINTKTGEFDTFRRWLVVDEVTNPTKEITLEAA
QFDDPNVQLGDYVEDQIESVAFDRITMQTARQVISTKIREAERNKIVEQFRSQEGEIVTGTVKKVNRDNIVLDLGQQAEA
VILREDMLPRENFRPGDRVRGVLYKVSPESKGAQLFVTRAKPEMLIELFRIEVPEIGEELIEIKNAARDPGSRAKIAVKS
NDKRIDPVGACVGMRGARVQAITNELGGERVDIVLWDDNPAQFVINAMAPADVTSIVVDEDSHAMDIAVEPANLAQAIGR
NGQNVRLATQLTSWTLNVMTTDELNQKHQAEDNKVLKLFMNALEIDEDFAHILIEEGFSTLEELAYVSVSELTAIDGLED
EDLVEELQARAKNAITAAALAEEEALKQAHIEDRLLALEGMNRHIALKLAEKQITTLEELAEQGVDDLADIEDLTAEQAA
DFIMAARNICWFSE

Sequences:

>Translated_494_residues
MSKELLLAAEAVSNEKLLPREKIFEALESALALSTKKKYEQEIDVRVVINTKTGEFDTFRRWLVVDEVTNPTKEITLEAA
QFDDPNVQLGDYVEDQIESVAFDRITMQTARQVISTKIREAERNKIVEQFRSQEGEIVTGTVKKVNRDNIVLDLGQQAEA
VILREDMLPRENFRPGDRVRGVLYKVSPESKGAQLFVTRAKPEMLIELFRIEVPEIGEELIEIKNAARDPGSRAKIAVKS
NDKRIDPVGACVGMRGARVQAITNELGGERVDIVLWDDNPAQFVINAMAPADVTSIVVDEDSHAMDIAVEPANLAQAIGR
NGQNVRLATQLTSWTLNVMTTDELNQKHQAEDNKVLKLFMNALEIDEDFAHILIEEGFSTLEELAYVSVSELTAIDGLED
EDLVEELQARAKNAITAAALAEEEALKQAHIEDRLLALEGMNRHIALKLAEKQITTLEELAEQGVDDLADIEDLTAEQAA
DFIMAARNICWFSE
>Mature_493_residues
SKELLLAAEAVSNEKLLPREKIFEALESALALSTKKKYEQEIDVRVVINTKTGEFDTFRRWLVVDEVTNPTKEITLEAAQ
FDDPNVQLGDYVEDQIESVAFDRITMQTARQVISTKIREAERNKIVEQFRSQEGEIVTGTVKKVNRDNIVLDLGQQAEAV
ILREDMLPRENFRPGDRVRGVLYKVSPESKGAQLFVTRAKPEMLIELFRIEVPEIGEELIEIKNAARDPGSRAKIAVKSN
DKRIDPVGACVGMRGARVQAITNELGGERVDIVLWDDNPAQFVINAMAPADVTSIVVDEDSHAMDIAVEPANLAQAIGRN
GQNVRLATQLTSWTLNVMTTDELNQKHQAEDNKVLKLFMNALEIDEDFAHILIEEGFSTLEELAYVSVSELTAIDGLEDE
DLVEELQARAKNAITAAALAEEEALKQAHIEDRLLALEGMNRHIALKLAEKQITTLEELAEQGVDDLADIEDLTAEQAAD
FIMAARNICWFSE

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=494, Percent_Identity=74.8987854251012, Blast_Score=748, Evalue=0.0,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 55146; Mature: 55015

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKELLLAAEAVSNEKLLPREKIFEALESALALSTKKKYEQEIDVRVVINTKTGEFDTFR
CCCCCEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHH
RWLVVDEVTNPTKEITLEAAQFDDPNVQLGDYVEDQIESVAFDRITMQTARQVISTKIRE
HEEEEECCCCCCHHEEEEEECCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AERNKIVEQFRSQEGEIVTGTVKKVNRDNIVLDLGQQAEAVILREDMLPRENFRPGDRVR
HHHHHHHHHHHCCCCCEEEEEHHHCCCCCEEEECCCCCCEEEEHHHCCCCCCCCCCHHHC
GVLYKVSPESKGAQLFVTRAKPEMLIELFRIEVPEIGEELIEIKNAARDPGSRAKIAVKS
EEEEEECCCCCCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEEEC
NDKRIDPVGACVGMRGARVQAITNELGGERVDIVLWDDNPAQFVINAMAPADVTSIVVDE
CCCCCCHHHHHHCCCCCHHHHHHHHCCCCEEEEEEECCCCHHEEEEECCCCCEEEEEEEC
DSHAMDIAVEPANLAQAIGRNGQNVRLATQLTSWTLNVMTTDELNQKHQAEDNKVLKLFM
CCCEEEEEECCHHHHHHHCCCCCCEEEEEEEEEEEEEEEECHHHHHHHCCCHHHHHHHHH
NALEIDEDFAHILIEEGFSTLEELAYVSVSELTAIDGLEDEDLVEELQARAKNAITAAAL
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
AEEEALKQAHIEDRLLALEGMNRHIALKLAEKQITTLEELAEQGVDDLADIEDLTAEQAA
HHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
DFIMAARNICWFSE
HHHHHHHCCCCCCC
>Mature Secondary Structure 
SKELLLAAEAVSNEKLLPREKIFEALESALALSTKKKYEQEIDVRVVINTKTGEFDTFR
CCCCEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHH
RWLVVDEVTNPTKEITLEAAQFDDPNVQLGDYVEDQIESVAFDRITMQTARQVISTKIRE
HEEEEECCCCCCHHEEEEEECCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AERNKIVEQFRSQEGEIVTGTVKKVNRDNIVLDLGQQAEAVILREDMLPRENFRPGDRVR
HHHHHHHHHHHCCCCCEEEEEHHHCCCCCEEEECCCCCCEEEEHHHCCCCCCCCCCHHHC
GVLYKVSPESKGAQLFVTRAKPEMLIELFRIEVPEIGEELIEIKNAARDPGSRAKIAVKS
EEEEEECCCCCCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEEEC
NDKRIDPVGACVGMRGARVQAITNELGGERVDIVLWDDNPAQFVINAMAPADVTSIVVDE
CCCCCCHHHHHHCCCCCHHHHHHHHCCCCEEEEEEECCCCHHEEEEECCCCCEEEEEEEC
DSHAMDIAVEPANLAQAIGRNGQNVRLATQLTSWTLNVMTTDELNQKHQAEDNKVLKLFM
CCCEEEEEECCHHHHHHHCCCCCCEEEEEEEEEEEEEEEECHHHHHHHCCCHHHHHHHHH
NALEIDEDFAHILIEEGFSTLEELAYVSVSELTAIDGLEDEDLVEELQARAKNAITAAAL
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
AEEEALKQAHIEDRLLALEGMNRHIALKLAEKQITTLEELAEQGVDDLADIEDLTAEQAA
HHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
DFIMAARNICWFSE
HHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]