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The map label for this gene is ttrB [H]

Identifier: 15602588

GI number: 15602588

Start: 853269

End: 854006

Strand: Reverse

Name: ttrB [H]

Synonym: PM0723

Alternate gene names: 15602588

Gene position: 854006-853269 (Counterclockwise)

Preceding gene: 15602591

Following gene: 15602587

Centisome position: 37.83

GC content: 41.06

Gene sequence:

>738_bases
ATGAAAATGAGTAAGCGTATTTTTCTCAAAAATTTATCATTATTAAGTGTGGGGCAAGCTTTTATTCCATTGAGTCAAGC
AGAAAATGCTTTTCAACCTGCTAGACGAGAGGGAAGTGAAGCACATCGTTATGCCATGTTGGTGGATTTACGTCGTTGTA
TCGGTTGTCAATCTTGTACTGTAAGTTGTGGGATTGAGAATGCGACGCCGATTGGCGAATTTAGAACCACTGTGCGTCAA
TACGAAGTCACTGATGAAAAACAAGTGGTCAATAATGTGTTATTACCACGTTTATGTAATCACTGTGATAACCCGCCTTG
TGTACCAGTTTGTCCTGTGCAAGCTACCTATCAACGTAAAGATGGTATCGTGGTGGTTGATAATGAACGTTGTATAGGTT
GTGCGTATTGTGTACAAGCTTGTCCTTATGATGCACGTTTTATTAACGAAGAAACAAAAACAGCAGATAAATGCACGTTT
TGCACACATCGACTGGAGGTTGGTTTATTACCTGCTTGTGTGGAAAGCTGTGTGGGTGGCGCGCGGATTATCGGCGATTT
ACGCGATCCAAATAGTACGATCAGCAAAATGGTAGAAGAATTTAAAGCGGAACTCAAAGTCTTAAAACCAAATTCGGGTA
CAGTGCCGCATGTATTCTATTTAGGATTAGATGACGCCTTTGTTAATCAAGTGCAGGGGCAATCTATGTTATGGCAAAGT
GCGGAGGGAAGATTATGA

Upstream 100 bases:

>100_bases
TGGAAAACCACAATAGTTTTTTCGCCGTCCTTCCTGAACAATACGAGAAAAAAATTATAACGCAATTGCGCGAAGAATTA
TAACTAAGAAGGAGCTAACC

Downstream 100 bases:

>100_bases
TCATTCGTGAAATCGTGGTGGAACCGCAAGCTATTGCATGGTTACCTTGGGCGGTGAGCTATTTCTTTTTTATTGGTCTC
GCCTTTTCTAGTGTATTTGT

Product: TTRB

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: Hme subunit A [H]

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MKMSKRIFLKNLSLLSVGQAFIPLSQAENAFQPARREGSEAHRYAMLVDLRRCIGCQSCTVSCGIENATPIGEFRTTVRQ
YEVTDEKQVVNNVLLPRLCNHCDNPPCVPVCPVQATYQRKDGIVVVDNERCIGCAYCVQACPYDARFINEETKTADKCTF
CTHRLEVGLLPACVESCVGGARIIGDLRDPNSTISKMVEEFKAELKVLKPNSGTVPHVFYLGLDDAFVNQVQGQSMLWQS
AEGRL

Sequences:

>Translated_245_residues
MKMSKRIFLKNLSLLSVGQAFIPLSQAENAFQPARREGSEAHRYAMLVDLRRCIGCQSCTVSCGIENATPIGEFRTTVRQ
YEVTDEKQVVNNVLLPRLCNHCDNPPCVPVCPVQATYQRKDGIVVVDNERCIGCAYCVQACPYDARFINEETKTADKCTF
CTHRLEVGLLPACVESCVGGARIIGDLRDPNSTISKMVEEFKAELKVLKPNSGTVPHVFYLGLDDAFVNQVQGQSMLWQS
AEGRL
>Mature_245_residues
MKMSKRIFLKNLSLLSVGQAFIPLSQAENAFQPARREGSEAHRYAMLVDLRRCIGCQSCTVSCGIENATPIGEFRTTVRQ
YEVTDEKQVVNNVLLPRLCNHCDNPPCVPVCPVQATYQRKDGIVVVDNERCIGCAYCVQACPYDARFINEETKTADKCTF
CTHRLEVGLLPACVESCVGGARIIGDLRDPNSTISKMVEEFKAELKVLKPNSGTVPHVFYLGLDDAFVNQVQGQSMLWQS
AEGRL

Specific function: Has menaquinol-oxidizing activity. HmeA, hmeB and hmeE subunits may together catalyze electron transfer from menaquinol to cytochrome c [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Extracellular side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=237, Percent_Identity=36.7088607594937, Blast_Score=137, Evalue=9e-34,
Organism=Escherichia coli, GI226510944, Length=213, Percent_Identity=34.7417840375587, Blast_Score=127, Evalue=6e-31,
Organism=Escherichia coli, GI1787872, Length=189, Percent_Identity=36.5079365079365, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787122, Length=189, Percent_Identity=35.978835978836, Blast_Score=107, Evalue=5e-25,
Organism=Escherichia coli, GI1790326, Length=215, Percent_Identity=29.7674418604651, Blast_Score=103, Evalue=9e-24,
Organism=Escherichia coli, GI1787749, Length=215, Percent_Identity=29.3023255813954, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1787740, Length=124, Percent_Identity=39.5161290322581, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787478, Length=96, Percent_Identity=44.7916666666667, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1789370, Length=236, Percent_Identity=30.5084745762712, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787963, Length=195, Percent_Identity=31.2820512820513, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI2367245, Length=141, Percent_Identity=36.1702127659575, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87082179, Length=139, Percent_Identity=32.3741007194245, Blast_Score=68, Evalue=4e-13,
Organism=Escherichia coli, GI87082180, Length=130, Percent_Identity=33.8461538461538, Blast_Score=68, Evalue=4e-13,
Organism=Escherichia coli, GI1789067, Length=169, Percent_Identity=31.3609467455621, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788811, Length=144, Percent_Identity=29.1666666666667, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 27198; Mature: 27198

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
8.6 %Cys+Met (Translated Protein)
6.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
8.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMSKRIFLKNLSLLSVGQAFIPLSQAENAFQPARREGSEAHRYAMLVDLRRCIGCQSCT
CCCCHHHHHHCCHHHHHCHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCE
VSCGIENATPIGEFRTTVRQYEVTDEKQVVNNVLLPRLCNHCDNPPCVPVCPVQATYQRK
EECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCEECCCCHHHCCC
DGIVVVDNERCIGCAYCVQACPYDARFINEETKTADKCTFCTHRLEVGLLPACVESCVGG
CCEEEEECCCCHHHHHHHHHCCCCHHHCCCHHCCHHHHHHHHHHHHHCCHHHHHHHHCCC
ARIIGDLRDPNSTISKMVEEFKAELKVLKPNSGTVPHVFYLGLDDAFVNQVQGQSMLWQS
CEEEECCCCCHHHHHHHHHHHHHHHEEECCCCCCCCEEEEEECCHHHHHHHCCHHHHHCC
AEGRL
CCCCC
>Mature Secondary Structure
MKMSKRIFLKNLSLLSVGQAFIPLSQAENAFQPARREGSEAHRYAMLVDLRRCIGCQSCT
CCCCHHHHHHCCHHHHHCHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCE
VSCGIENATPIGEFRTTVRQYEVTDEKQVVNNVLLPRLCNHCDNPPCVPVCPVQATYQRK
EECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCEECCCCHHHCCC
DGIVVVDNERCIGCAYCVQACPYDARFINEETKTADKCTFCTHRLEVGLLPACVESCVGG
CCEEEEECCCCHHHHHHHHHCCCCHHHCCCHHCCHHHHHHHHHHHHHCCHHHHHHHHCCC
ARIIGDLRDPNSTISKMVEEFKAELKVLKPNSGTVPHVFYLGLDDAFVNQVQGQSMLWQS
CEEEECCCCCHHHHHHHHHHHHHHHEEECCCCCCCCEEEEEECCHHHHHHHCCHHHHHCC
AEGRL
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9389475; 11952791 [H]