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The map label for this gene is greA

Identifier: 15602580

GI number: 15602580

Start: 840742

End: 841218

Strand: Reverse

Name: greA

Synonym: PM0715

Alternate gene names: 15602580

Gene position: 841218-840742 (Counterclockwise)

Preceding gene: 15602586

Following gene: 15602579

Centisome position: 37.26

GC content: 39.41

Gene sequence:

>477_bases
ATGAAACAGATTCCAATGACTATACGTGGTGCGGAACAATTAAAACAAGAACTCGATTTTTTGAAAAACACTCGTCGCCC
AGAAATTATTAATGCTATCGCAGAAGCTCGTGAACATGGCGATCTAAAAGAAAATGCAGAATACCATGCTGCGCGTGAAC
AGCAAGGATTTTGTGAAGGACGAATCCAAGAAATTGAAGGAAAATTAGCGAATAGTCAAATTATTGACGTCACAAAGATC
CCGAATAATGGCAAAGTGATTTTTGGTGCCACAATTTTGTTACTGAATATTGACACGGAAGAAGAAGTCTCGTACCAAAT
TGTAGGCGATGATGAAGCCAATATTAAAGCAGGGCTAATTTCCGTTAACTCACCGATTGCGCGAGGGTTAATTGGCAAAG
AGGTGGATGACGTTGTTGTTATTCAAACGCCGGGCGGCAAAGTGGAGTTTGAAATTCTTGAAGTTGAATATAAATAA

Upstream 100 bases:

>100_bases
AATCCTTTTCTCGTTAAACACTTATTTTTTTATTCAACTACGGCATTGTTTTTACAATGTTGTGGTTTTGTTTTTATCTA
AAAAGGAAGAAAAAACGATT

Downstream 100 bases:

>100_bases
GTAAAAAACGCCCAGAAAATAAAAGCCTTATTTGTTGACAATAAGGCTTTTTTACATCCACAATAAATGACTCTAATCTC
TGTAAAGAAAGGTAACTAAT

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MKQIPMTIRGAEQLKQELDFLKNTRRPEIINAIAEAREHGDLKENAEYHAAREQQGFCEGRIQEIEGKLANSQIIDVTKI
PNNGKVIFGATILLLNIDTEEEVSYQIVGDDEANIKAGLISVNSPIARGLIGKEVDDVVVIQTPGGKVEFEILEVEYK

Sequences:

>Translated_158_residues
MKQIPMTIRGAEQLKQELDFLKNTRRPEIINAIAEAREHGDLKENAEYHAAREQQGFCEGRIQEIEGKLANSQIIDVTKI
PNNGKVIFGATILLLNIDTEEEVSYQIVGDDEANIKAGLISVNSPIARGLIGKEVDDVVVIQTPGGKVEFEILEVEYK
>Mature_158_residues
MKQIPMTIRGAEQLKQELDFLKNTRRPEIINAIAEAREHGDLKENAEYHAAREQQGFCEGRIQEIEGKLANSQIIDVTKI
PNNGKVIFGATILLLNIDTEEEVSYQIVGDDEANIKAGLISVNSPIARGLIGKEVDDVVVIQTPGGKVEFEILEVEYK

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=157, Percent_Identity=77.0700636942675, Blast_Score=260, Evalue=3e-71,
Organism=Escherichia coli, GI87082261, Length=157, Percent_Identity=35.6687898089172, Blast_Score=96, Evalue=1e-21,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_PASMU (P57801)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245652.1
- ProteinModelPortal:   P57801
- SMR:   P57801
- GeneID:   1244062
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0715
- NMPDR:   fig|272843.1.peg.715
- HOGENOM:   HBG753387
- OMA:   HNEGRIA
- ProtClustDB:   PRK00226
- BioCyc:   PMUL272843:PM0715-MONOMER
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 17556; Mature: 17556

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQIPMTIRGAEQLKQELDFLKNTRRPEIINAIAEAREHGDLKENAEYHAAREQQGFCEG
CCCCCCEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHH
RIQEIEGKLANSQIIDVTKIPNNGKVIFGATILLLNIDTEEEVSYQIVGDDEANIKAGLI
HHHHHCCCCCCCEEEEEEECCCCCEEEEEEEEEEEECCCCCCEEEEEECCCCCCEEEEEE
SVNSPIARGLIGKEVDDVVVIQTPGGKVEFEILEVEYK
ECCCHHHHCCCCCCCCCEEEEECCCCEEEEEEEEEEEC
>Mature Secondary Structure
MKQIPMTIRGAEQLKQELDFLKNTRRPEIINAIAEAREHGDLKENAEYHAAREQQGFCEG
CCCCCCEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHH
RIQEIEGKLANSQIIDVTKIPNNGKVIFGATILLLNIDTEEEVSYQIVGDDEANIKAGLI
HHHHHCCCCCCCEEEEEEECCCCCEEEEEEEEEEEECCCCCCEEEEEECCCCCCEEEEEE
SVNSPIARGLIGKEVDDVVVIQTPGGKVEFEILEVEYK
ECCCHHHHCCCCCCCCCEEEEECCCCEEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100