Click here to switch to the map view.

The map label for this gene is purF

Identifier: 15602566

GI number: 15602566

Start: 817002

End: 818516

Strand: Reverse

Name: purF

Synonym: PM0701

Alternate gene names: 15602566

Gene position: 818516-817002 (Counterclockwise)

Preceding gene: 15602567

Following gene: 15602565

Centisome position: 36.26

GC content: 41.25

Gene sequence:

>1515_bases
ATGTGTGGTATTGTTGGTATTGTTAGCCAAAGCCCAGTTAACCAATCAATTTATGATGCGTTAACCTTATTGCAACACCG
CGGGCAAGACGCCGCCGGGATTGTAACCGTAGATGATGAAAACCGATTCCGCTTGCGTAAAGCGAACGGGTTAGTCAGCG
ATGTATTTGAACAAGTTCATATGTTACGTTTACAAGGCAATGCTGGCATTGGACATGTTCGTTATCCTACTGCTGGGAGC
TCAAGTGTCTCTGAAGCGCAACCTTTTTATGTAAATTCGCCTTATGGCTTAACCTTAGTGCATAATGGTAACTTGACCAA
TTCAAGTGAATTAAAAGAAAAGTTATTTCGTCGCGCGCGTCGCCATGTAAATACCAATTCAGATTCTGAATTATTACTCA
ATATCTTAGCCAATCACCTTGATCACTTCGAAAAATACCAATTAGATCCGCAAGATGTATTTAGTGCTGTCAAACAAACG
CATCAGGATATTCGTGGTGCTTATGCTTGTATCGCCATGATTATTGGTCATGGTATGGTCGCGTTTCGCGATCCGAACGG
TATCCGTCCATTAGTGTTAGGGAAACGCGAGGAAAATGGCAAAACAGAGTATATGTTTGCCTCCGAAAGTATCGCATTAG
ATACAGTGGGTTTTGAATTTGTACGAGATGTACAACCCGGCGAAGCGATTTATGTCACGTTTGAAGGGGAAATGTATGCC
CAGCAATGTGCTGACAAACCAACATTAACACCTTGTATTTTTGAATATGTCTATTTTGCACGTCCAGACTCCTGCATCGA
TGGGGTTTCTGTTTATGCTGCCCGTGTTCATATGGGACAGCGTTTAGGTGAAAAAATTGCACGGGAATGGGCGGATGTGG
ATGATATTGATGTGGTCATTCCTGTGCCTGAAACCTCTAACGATATTGCTTTACGTATTGCGCGCGTGTTAAATAAACCG
TATCGTCAAGGTTTTGTGAAAAATCGCTATGTGGGGCGTACGTTTATTATGCCGGGTCAGGCATTGCGAGTCAGTTCTGT
TAGACGTAAACTCAATACCATTGCTTCAGAATTTAAAGATAAGAATGTGTTATTAGTTGACGACTCGATTGTACGTGGTA
CCACGTCTGAACAAATTGTCGAAATGGCGAGAGCGGCAGGTGCGAAGAAAATTTATTTTGCCTCTGCTGCACCGGAAATT
CGTTATCCAAATGTGTATGGTATTGATATGCCGACCAAAAATGAATTGATCGCTTATGGTCGTGATGTAGATGAAATTGC
TAAATTAATTGGTGTGGATAAATTGATTTTCCAAGATTTGGATGCGTTAACCGGTTCTGTGCAACAAGAAAATCCAAGTA
TTCAAGACTTTGATTGTTCGGTGTTTACAGGGGTTTATGTGACGGGTGATATTACACCTGAATATCTGGATAATATTGCA
GAACAGCGTAATGATATCGCCAAGAAAAAGCGTGAAAAAGATGCTACCAATCTTGAAATGCACAATGAAAAATAA

Upstream 100 bases:

>100_bases
ATTCCACATTTCGGCTTTATTGTTGAGTGGTTTTTTCAACAGTTACAAGCAACATCCAGTTTTATAAATTCAACATTAAA
TAAATAGAGGATAAACACGA

Downstream 100 bases:

>100_bases
GCCTTTTTGATAACAAAAATGGAGAGTTGAATGAATTTGACTCTCCATTTTTATTTTTCCATTTTCTGCTTTTCTTTCAC
TTAAATTGTGAGGAACACAT

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MCGIVGIVSQSPVNQSIYDALTLLQHRGQDAAGIVTVDDENRFRLRKANGLVSDVFEQVHMLRLQGNAGIGHVRYPTAGS
SSVSEAQPFYVNSPYGLTLVHNGNLTNSSELKEKLFRRARRHVNTNSDSELLLNILANHLDHFEKYQLDPQDVFSAVKQT
HQDIRGAYACIAMIIGHGMVAFRDPNGIRPLVLGKREENGKTEYMFASESIALDTVGFEFVRDVQPGEAIYVTFEGEMYA
QQCADKPTLTPCIFEYVYFARPDSCIDGVSVYAARVHMGQRLGEKIAREWADVDDIDVVIPVPETSNDIALRIARVLNKP
YRQGFVKNRYVGRTFIMPGQALRVSSVRRKLNTIASEFKDKNVLLVDDSIVRGTTSEQIVEMARAAGAKKIYFASAAPEI
RYPNVYGIDMPTKNELIAYGRDVDEIAKLIGVDKLIFQDLDALTGSVQQENPSIQDFDCSVFTGVYVTGDITPEYLDNIA
EQRNDIAKKKREKDATNLEMHNEK

Sequences:

>Translated_504_residues
MCGIVGIVSQSPVNQSIYDALTLLQHRGQDAAGIVTVDDENRFRLRKANGLVSDVFEQVHMLRLQGNAGIGHVRYPTAGS
SSVSEAQPFYVNSPYGLTLVHNGNLTNSSELKEKLFRRARRHVNTNSDSELLLNILANHLDHFEKYQLDPQDVFSAVKQT
HQDIRGAYACIAMIIGHGMVAFRDPNGIRPLVLGKREENGKTEYMFASESIALDTVGFEFVRDVQPGEAIYVTFEGEMYA
QQCADKPTLTPCIFEYVYFARPDSCIDGVSVYAARVHMGQRLGEKIAREWADVDDIDVVIPVPETSNDIALRIARVLNKP
YRQGFVKNRYVGRTFIMPGQALRVSSVRRKLNTIASEFKDKNVLLVDDSIVRGTTSEQIVEMARAAGAKKIYFASAAPEI
RYPNVYGIDMPTKNELIAYGRDVDEIAKLIGVDKLIFQDLDALTGSVQQENPSIQDFDCSVFTGVYVTGDITPEYLDNIA
EQRNDIAKKKREKDATNLEMHNEK
>Mature_504_residues
MCGIVGIVSQSPVNQSIYDALTLLQHRGQDAAGIVTVDDENRFRLRKANGLVSDVFEQVHMLRLQGNAGIGHVRYPTAGS
SSVSEAQPFYVNSPYGLTLVHNGNLTNSSELKEKLFRRARRHVNTNSDSELLLNILANHLDHFEKYQLDPQDVFSAVKQT
HQDIRGAYACIAMIIGHGMVAFRDPNGIRPLVLGKREENGKTEYMFASESIALDTVGFEFVRDVQPGEAIYVTFEGEMYA
QQCADKPTLTPCIFEYVYFARPDSCIDGVSVYAARVHMGQRLGEKIAREWADVDDIDVVIPVPETSNDIALRIARVLNKP
YRQGFVKNRYVGRTFIMPGQALRVSSVRRKLNTIASEFKDKNVLLVDDSIVRGTTSEQIVEMARAAGAKKIYFASAAPEI
RYPNVYGIDMPTKNELIAYGRDVDEIAKLIGVDKLIFQDLDALTGSVQQENPSIQDFDCSVFTGVYVTGDITPEYLDNIA
EQRNDIAKKKREKDATNLEMHNEK

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain

Homologues:

Organism=Homo sapiens, GI29570798, Length=474, Percent_Identity=36.2869198312236, Blast_Score=268, Evalue=9e-72,
Organism=Escherichia coli, GI1788651, Length=505, Percent_Identity=72.4752475247525, Blast_Score=763, Evalue=0.0,
Organism=Escherichia coli, GI1790167, Length=224, Percent_Identity=32.1428571428571, Blast_Score=82, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17554892, Length=465, Percent_Identity=33.7634408602151, Blast_Score=227, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6323958, Length=502, Percent_Identity=51.394422310757, Blast_Score=502, Evalue=1e-143,
Organism=Drosophila melanogaster, GI24659598, Length=504, Percent_Identity=33.9285714285714, Blast_Score=259, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24659604, Length=480, Percent_Identity=34.5833333333333, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI28573187, Length=503, Percent_Identity=33.3996023856859, Blast_Score=253, Evalue=3e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUR1_PASMU (Q9L6B8)

Other databases:

- EMBL:   AF237920
- EMBL:   AE004439
- RefSeq:   NP_245638.1
- ProteinModelPortal:   Q9L6B8
- SMR:   Q9L6B8
- GeneID:   1244048
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0701
- NMPDR:   fig|272843.1.peg.701
- HOGENOM:   HBG392416
- OMA:   SYCTACF
- ProtClustDB:   PRK09246
- BioCyc:   PMUL272843:PM0701-MONOMER
- BRENDA:   2.4.2.14
- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836
- PANTHER:   PTHR11907
- PIRSF:   PIRSF000485
- TIGRFAMs:   TIGR01134

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran

EC number: =2.4.2.14

Molecular weight: Translated: 56328; Mature: 56328

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS51278 GATASE_TYPE_2; PS00103 PUR_PYR_PR_TRANSFER; PS00443 GATASE_TYPE_II

Important sites: ACT_SITE 2-2

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGIVSQSPVNQSIYDALTLLQHRGQDAAGIVTVDDENRFRLRKANGLVSDVFEQVH
CCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEHHCCHHHHHHHHHH
MLRLQGNAGIGHVRYPTAGSSSVSEAQPFYVNSPYGLTLVHNGNLTNSSELKEKLFRRAR
HEEEECCCCCCEEECCCCCCCCCCCCCCEEEECCCCEEEEECCCCCCHHHHHHHHHHHHH
RHVNTNSDSELLLNILANHLDHFEKYQLDPQDVFSAVKQTHQDIRGAYACIAMIIGHGMV
HHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
AFRDPNGIRPLVLGKREENGKTEYMFASESIALDTVGFEFVRDVQPGEAIYVTFEGEMYA
EEECCCCCCCEEECCCCCCCCEEEEEECCCEEEEHHHHHHHHCCCCCCEEEEEECCCHHH
QQCADKPTLTPCIFEYVYFARPDSCIDGVSVYAARVHMGQRLGEKIAREWADVDDIDVVI
HHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
PVPETSNDIALRIARVLNKPYRQGFVKNRYVGRTFIMPGQALRVSSVRRKLNTIASEFKD
ECCCCCCHHHHHHHHHHCCHHHHCHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCC
KNVLLVDDSIVRGTTSEQIVEMARAAGAKKIYFASAAPEIRYPNVYGIDMPTKNELIAYG
CCEEEECCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCCCCEECC
RDVDEIAKLIGVDKLIFQDLDALTGSVQQENPSIQDFDCSVFTGVYVTGDITPEYLDNIA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHH
EQRNDIAKKKREKDATNLEMHNEK
HHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MCGIVGIVSQSPVNQSIYDALTLLQHRGQDAAGIVTVDDENRFRLRKANGLVSDVFEQVH
CCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEHHCCHHHHHHHHHH
MLRLQGNAGIGHVRYPTAGSSSVSEAQPFYVNSPYGLTLVHNGNLTNSSELKEKLFRRAR
HEEEECCCCCCEEECCCCCCCCCCCCCCEEEECCCCEEEEECCCCCCHHHHHHHHHHHHH
RHVNTNSDSELLLNILANHLDHFEKYQLDPQDVFSAVKQTHQDIRGAYACIAMIIGHGMV
HHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
AFRDPNGIRPLVLGKREENGKTEYMFASESIALDTVGFEFVRDVQPGEAIYVTFEGEMYA
EEECCCCCCCEEECCCCCCCCEEEEEECCCEEEEHHHHHHHHCCCCCCEEEEEECCCHHH
QQCADKPTLTPCIFEYVYFARPDSCIDGVSVYAARVHMGQRLGEKIAREWADVDDIDVVI
HHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
PVPETSNDIALRIARVLNKPYRQGFVKNRYVGRTFIMPGQALRVSSVRRKLNTIASEFKD
ECCCCCCHHHHHHHHHHCCHHHHCHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCC
KNVLLVDDSIVRGTTSEQIVEMARAAGAKKIYFASAAPEIRYPNVYGIDMPTKNELIAYG
CCEEEECCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCCCCEECC
RDVDEIAKLIGVDKLIFQDLDALTGSVQQENPSIQDFDCSVFTGVYVTGDITPEYLDNIA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHH
EQRNDIAKKKREKDATNLEMHNEK
HHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10873488; 11248100