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The map label for this gene is pykA [H]

Identifier: 15602518

GI number: 15602518

Start: 755890

End: 757329

Strand: Reverse

Name: pykA [H]

Synonym: PM0653

Alternate gene names: 15602518

Gene position: 757329-755890 (Counterclockwise)

Preceding gene: 15602519

Following gene: 15602515

Centisome position: 33.55

GC content: 39.79

Gene sequence:

>1440_bases
ATGTCTAGAAGACTAAGAAGAACGAAGATTGTATGTACAATGGGACCGTCAACAGAGCGTGGGAATAATCTTGAAAAAAT
TATCATGGCTGGCGCGAATGTTGTTCGTATGAACTTTTCTCATGGCACACCAGAAGATCATATCGGTCGCGCGGAGAAAG
TACGTGAAGTGGCAAAAAAACTTGGAAAAACCGTTGCTATTTTAGGTGATTTACAAGGCCCTAAAATCCGTGTCTCAACT
TTCAAAGATGGTAAAATTTTCTTAAATATTGGTGATAAATTTGTGCTTGATGCCGATTTACCAAAAGGTGAAGGTACGCA
AGAAGGTGTTGGTTTAGACTACAAAACATTACCACAAGACGTTGTGCCAGGCGATATTTTATTGTTAGATGATGGTCGTG
TTCAATTAAAAGTCTTATCTACAGAAGGTAATAAAGTTTTTACTGAAGTCACTGTTGGTGGTCCATTATCGAACAATAAA
GGTATTAACAAGTTAGGCGGAGGTTTATCTGCTGATGCATTAACTGAAAAAGATAAAAATGATATTTTATTGGCAGCAAA
AATCGGTGTAGATTATTTAGCGGTTTCTTTCCCTCGTTCAAGTGCAGATTTAAACTATGCGCGTGAGTTAGCTGAAGCTG
CAGGCTTAAAAGCAAAAATCGTGGCAAAAGTAGAACGTGCAGAAACTGTTGCAACCACTGAAGCAATGGATGATATTATT
TTAGCATCAGATGTCATCATGGTAGCACGTGGTGACTTAGGGGTTGAAATTGGTGACCCTGAATTAGTGGGTGTACAGAA
AAAATTAATCCGTCGCGCACGCCAATTAAACCGTGTTGTTATTACAGCAACACAAATGATGGAATCTATGATTAGCAATC
CAATGCCGACTCGTGCGGAAGTGATGGACGTAGCGAATGCCGTATTAGATGGTACAGACGCCGTGATGCTTTCTGCAGAA
ACAGCGGCAGGGCAATATCCAGCAGAAACAGTGGCTACCATGGCAAAAGTGTGTTTAGGTGCTGAAAAAATGCCAAGCAT
CAATGTTTCTCGTCACCGTATGGATATCACTTTTGATTCTATCGAAGAATCCGTTGCAATGTCTGCAATGTATGCTGCAA
ACCACATGAAAGGTGTTGCTGCAATTATTGCAATGACACATACTGGACGTACCGCGTTATTAATGTCACGTATTAGTTCT
GGTTTACCGATTTTTGCCTTATCTCGTAACCAAGATGCATTAAATCTTTGTGCATTATACCGTGGTGTTACACCTGTTCA
TTTTGATGAAGTCAGCCGTAATTCAAAAGGTGCAGCTGCAGCGATTCGTTTGTTAAAAGAGAAAGGTTTCTTAGTATCGG
GTGATTTAGTTCTTTTAACTCAAGGTGATGAATTGTTAGCAAGTGGTACAAATACTTGCCGTACACTAGTTGTAGAATAA

Upstream 100 bases:

>100_bases
TTTAGGCGTATAATAAGGCACAGTTTTTTCTTTCAAGCTTGAATTATGTAAGAAAGCTAAGATCACTATTTTATAACTTA
TATCATTTACGGAGTAGGTA

Downstream 100 bases:

>100_bases
ACAGTATTCTTAAAACAAAACGGTGATAAGCTAATGTTTATCACCGTTTTTTATTTTTTAAATAAAGTGTAACGATAAGA
AAATAGGCTATTTTAATGTT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MSRRLRRTKIVCTMGPSTERGNNLEKIIMAGANVVRMNFSHGTPEDHIGRAEKVREVAKKLGKTVAILGDLQGPKIRVST
FKDGKIFLNIGDKFVLDADLPKGEGTQEGVGLDYKTLPQDVVPGDILLLDDGRVQLKVLSTEGNKVFTEVTVGGPLSNNK
GINKLGGGLSADALTEKDKNDILLAAKIGVDYLAVSFPRSSADLNYARELAEAAGLKAKIVAKVERAETVATTEAMDDII
LASDVIMVARGDLGVEIGDPELVGVQKKLIRRARQLNRVVITATQMMESMISNPMPTRAEVMDVANAVLDGTDAVMLSAE
TAAGQYPAETVATMAKVCLGAEKMPSINVSRHRMDITFDSIEESVAMSAMYAANHMKGVAAIIAMTHTGRTALLMSRISS
GLPIFALSRNQDALNLCALYRGVTPVHFDEVSRNSKGAAAAIRLLKEKGFLVSGDLVLLTQGDELLASGTNTCRTLVVE

Sequences:

>Translated_479_residues
MSRRLRRTKIVCTMGPSTERGNNLEKIIMAGANVVRMNFSHGTPEDHIGRAEKVREVAKKLGKTVAILGDLQGPKIRVST
FKDGKIFLNIGDKFVLDADLPKGEGTQEGVGLDYKTLPQDVVPGDILLLDDGRVQLKVLSTEGNKVFTEVTVGGPLSNNK
GINKLGGGLSADALTEKDKNDILLAAKIGVDYLAVSFPRSSADLNYARELAEAAGLKAKIVAKVERAETVATTEAMDDII
LASDVIMVARGDLGVEIGDPELVGVQKKLIRRARQLNRVVITATQMMESMISNPMPTRAEVMDVANAVLDGTDAVMLSAE
TAAGQYPAETVATMAKVCLGAEKMPSINVSRHRMDITFDSIEESVAMSAMYAANHMKGVAAIIAMTHTGRTALLMSRISS
GLPIFALSRNQDALNLCALYRGVTPVHFDEVSRNSKGAAAAIRLLKEKGFLVSGDLVLLTQGDELLASGTNTCRTLVVE
>Mature_478_residues
SRRLRRTKIVCTMGPSTERGNNLEKIIMAGANVVRMNFSHGTPEDHIGRAEKVREVAKKLGKTVAILGDLQGPKIRVSTF
KDGKIFLNIGDKFVLDADLPKGEGTQEGVGLDYKTLPQDVVPGDILLLDDGRVQLKVLSTEGNKVFTEVTVGGPLSNNKG
INKLGGGLSADALTEKDKNDILLAAKIGVDYLAVSFPRSSADLNYARELAEAAGLKAKIVAKVERAETVATTEAMDDIIL
ASDVIMVARGDLGVEIGDPELVGVQKKLIRRARQLNRVVITATQMMESMISNPMPTRAEVMDVANAVLDGTDAVMLSAET
AAGQYPAETVATMAKVCLGAEKMPSINVSRHRMDITFDSIEESVAMSAMYAANHMKGVAAIIAMTHTGRTALLMSRISSG
LPIFALSRNQDALNLCALYRGVTPVHFDEVSRNSKGAAAAIRLLKEKGFLVSGDLVLLTQGDELLASGTNTCRTLVVE

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=497, Percent_Identity=35.4124748490946, Blast_Score=254, Evalue=8e-68,
Organism=Homo sapiens, GI33286420, Length=497, Percent_Identity=35.4124748490946, Blast_Score=254, Evalue=8e-68,
Organism=Homo sapiens, GI33286418, Length=493, Percent_Identity=35.6997971602434, Blast_Score=251, Evalue=8e-67,
Organism=Homo sapiens, GI10835121, Length=502, Percent_Identity=34.6613545816733, Blast_Score=237, Evalue=2e-62,
Organism=Homo sapiens, GI32967597, Length=502, Percent_Identity=34.6613545816733, Blast_Score=236, Evalue=3e-62,
Organism=Homo sapiens, GI310128732, Length=289, Percent_Identity=34.9480968858132, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI310128730, Length=289, Percent_Identity=34.9480968858132, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=32.7659574468085, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=32.7659574468085, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=32.2429906542056, Blast_Score=97, Evalue=5e-20,
Organism=Escherichia coli, GI1788160, Length=480, Percent_Identity=78.125, Blast_Score=764, Evalue=0.0,
Organism=Escherichia coli, GI1787965, Length=480, Percent_Identity=38.5416666666667, Blast_Score=295, Evalue=4e-81,
Organism=Caenorhabditis elegans, GI17544584, Length=490, Percent_Identity=36.9387755102041, Blast_Score=278, Evalue=6e-75,
Organism=Caenorhabditis elegans, GI17506829, Length=492, Percent_Identity=36.3821138211382, Blast_Score=248, Evalue=4e-66,
Organism=Caenorhabditis elegans, GI71984413, Length=492, Percent_Identity=36.3821138211382, Blast_Score=248, Evalue=4e-66,
Organism=Caenorhabditis elegans, GI71984406, Length=492, Percent_Identity=36.3821138211382, Blast_Score=248, Evalue=5e-66,
Organism=Caenorhabditis elegans, GI17506831, Length=492, Percent_Identity=36.3821138211382, Blast_Score=248, Evalue=6e-66,
Organism=Saccharomyces cerevisiae, GI6319279, Length=490, Percent_Identity=36.1224489795918, Blast_Score=250, Evalue=3e-67,
Organism=Saccharomyces cerevisiae, GI6324923, Length=487, Percent_Identity=32.4435318275154, Blast_Score=234, Evalue=2e-62,
Organism=Drosophila melanogaster, GI24648964, Length=493, Percent_Identity=36.9168356997972, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI28571814, Length=493, Percent_Identity=36.9168356997972, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24648966, Length=423, Percent_Identity=35.4609929078014, Blast_Score=237, Evalue=1e-62,
Organism=Drosophila melanogaster, GI24581235, Length=440, Percent_Identity=32.7272727272727, Blast_Score=221, Evalue=1e-57,
Organism=Drosophila melanogaster, GI24646914, Length=286, Percent_Identity=32.8671328671329, Blast_Score=139, Evalue=6e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51231; Mature: 51100

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRLRRTKIVCTMGPSTERGNNLEKIIMAGANVVRMNFSHGTPEDHIGRAEKVREVAKK
CCCCCCEEEEEEEECCCCCCCCCHHHHHHCCCEEEEEECCCCCCHHHCCHHHHHHHHHHH
LGKTVAILGDLQGPKIRVSTFKDGKIFLNIGDKFVLDADLPKGEGTQEGVGLDYKTLPQD
HCCEEEEEECCCCCEEEEEEECCCEEEEEECCEEEEECCCCCCCCCCCCCCCCHHCCCCC
VVPGDILLLDDGRVQLKVLSTEGNKVFTEVTVGGPLSNNKGINKLGGGLSADALTEKDKN
CCCCCEEEEECCCEEEEEEECCCCEEEEEEEECCCCCCCCCCHHHCCCCCCHHHCCCCCC
DILLAAKIGVDYLAVSFPRSSADLNYARELAEAAGLKAKIVAKVERAETVATTEAMDDII
CEEEEEECCCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHH
LASDVIMVARGDLGVEIGDPELVGVQKKLIRRARQLNRVVITATQMMESMISNPMPTRAE
HHCCEEEEEECCCCEEECCCCEEHHHHHHHHHHHHHCEEEEEHHHHHHHHHCCCCCCHHH
VMDVANAVLDGTDAVMLSAETAAGQYPAETVATMAKVCLGAEKMPSINVSRHRMDITFDS
HHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHH
IEESVAMSAMYAANHMKGVAAIIAMTHTGRTALLMSRISSGLPIFALSRNQDALNLCALY
HHHHHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHH
RGVTPVHFDEVSRNSKGAAAAIRLLKEKGFLVSGDLVLLTQGDELLASGTNTCRTLVVE
HCCCCEEHHHHCCCCCCHHHHHHHHHHCCCEEECCEEEEECCCHHHHCCCCCEEEEEEC
>Mature Secondary Structure 
SRRLRRTKIVCTMGPSTERGNNLEKIIMAGANVVRMNFSHGTPEDHIGRAEKVREVAKK
CCCCCEEEEEEEECCCCCCCCCHHHHHHCCCEEEEEECCCCCCHHHCCHHHHHHHHHHH
LGKTVAILGDLQGPKIRVSTFKDGKIFLNIGDKFVLDADLPKGEGTQEGVGLDYKTLPQD
HCCEEEEEECCCCCEEEEEEECCCEEEEEECCEEEEECCCCCCCCCCCCCCCCHHCCCCC
VVPGDILLLDDGRVQLKVLSTEGNKVFTEVTVGGPLSNNKGINKLGGGLSADALTEKDKN
CCCCCEEEEECCCEEEEEEECCCCEEEEEEEECCCCCCCCCCHHHCCCCCCHHHCCCCCC
DILLAAKIGVDYLAVSFPRSSADLNYARELAEAAGLKAKIVAKVERAETVATTEAMDDII
CEEEEEECCCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHH
LASDVIMVARGDLGVEIGDPELVGVQKKLIRRARQLNRVVITATQMMESMISNPMPTRAE
HHCCEEEEEECCCCEEECCCCEEHHHHHHHHHHHHHCEEEEEHHHHHHHHHCCCCCCHHH
VMDVANAVLDGTDAVMLSAETAAGQYPAETVATMAKVCLGAEKMPSINVSRHRMDITFDS
HHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHH
IEESVAMSAMYAANHMKGVAAIIAMTHTGRTALLMSRISSGLPIFALSRNQDALNLCALY
HHHHHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHH
RGVTPVHFDEVSRNSKGAAAAIRLLKEKGFLVSGDLVLLTQGDELLASGTNTCRTLVVE
HCCCCEEHHHHCCCCCCHHHHHHHHHHCCCEEECCEEEEECCCHHHHCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]