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The map label for this gene is folC [H]

Identifier: 15602500

GI number: 15602500

Start: 736710

End: 738038

Strand: Reverse

Name: folC [H]

Synonym: PM0635

Alternate gene names: 15602500

Gene position: 738038-736710 (Counterclockwise)

Preceding gene: 15602501

Following gene: 15602498

Centisome position: 32.69

GC content: 42.81

Gene sequence:

>1329_bases
ATGAATAATTCTACAGTATCATTAACAGCCACTTCGCCTCTTGCGCAGTGGCTTTCTTATTTGGAAAACAGCCATTTTAA
AGCCATTGATTTAGGGCTAGATAGAATTAAATCGGTTGCCAAGGCGCTGGATTTGCTCACGCCCGCACCATTTGTCATCA
CGGTGGGTGGTACTAATGGTAAAGGCACTACGTGTCGTTTATTGGAAACCGTTTTATTAAAGGCAGGCTACCGTGTTGGT
GTGTATTCTTCCCCTCATTTGCTTCGCTATAATGAACGCGTACGTATTCAAGACCAAGAATTGCCAGATGAAGCGCATAC
GGCTTCTTTTGCCTTTATTGAACAGCATAAACGTGAATCGTTAACCTATTTTGAGTTTAGCACCCTTTCTGCCTTGCATT
TATTTAAGCAAGCGAACTTGGATGTGGTGATTTTAGAAGTGGGGTTAGGGGGGCGTTTAGACGCGACTAATATTGTTGAT
AGTGATGTGGCGGTGATTACCAGTATAGACATTGATCATGTGGATTTTTTAGGGGCAGATCGCGAACAAATCGGGTTTGA
GAAAGCAGGCATTTTCCGTGCACATAAACCGGCGATTATTGGAGAGCCTAATATTCCTCAAAGATTATTGGAGCATGCTA
AGTCACTCGGATGCCAAATCTCTTGTCGTGATCAAGATTGGCATTTCCGTTTACAAGCGGAGCATTGGGATTGGTGTGGG
CAAAAAGTGCGGTTGAAAAATTTGCCATGTTGTCAAATTCCACTCGCTAATGCCGCAACAGCACTCGCTACATTTGAACA
GTTGCCTTTTCAGATTTCAGAAGAGGTGATTCGTCAATCTTTGCAAGAAGTGGAATTAGTTGGGCGTTTTCAAACCTTAA
AACCAGATGTCCTTTTCCCTTTAGCCGAAAATTTAGCGAAACCATGGGCTGATTTTCCACAAATGATTATTGATGTGGGG
CATAATCCACATGCAGCCCTTTATTTAGCAGAGAAACTGACCGCACTTAAGGCGAAAATAACAGGTAAGATCGTCGCTGT
TTGTGGCATTTTAAAAGATAAAGATGCACAAGGCGTGCTTTCTCCTTTGTTACCGGTGATTGATGAATGGCATTGCGTGA
CATTAGAAGGGTATCGAGGTCAAAGCGGACAGGCATTATTTGTGACGTTGCAACAAATTGCACAGGGACAATCTCATCAA
CTGCATGCAAACACTGCGAGCTCTGTAGCATTAGGTGTAAAACAAGCGTTGGCAACGACAACAGCGCATGATGTAATCTT
AGTCTTTGGCTCTTTCCATACTGTTGGCGAATTCTTACAGTTACTTTAA

Upstream 100 bases:

>100_bases
CGTCATACGTTAGCAAGTGTGTTAAGCAAATTAAGCAATAAACCATCGCCTTTTGTGGAACCTGAATTGGTGGAAAACGA
AGAACAAAGTAAAAGCGATA

Downstream 100 bases:

>100_bases
TACTCAATAAGGCAGAATAGACTTCTGCCTTTATTCCTCATCGAATGAATCGGTTAAATGCTGTATATCATTGACCTTTT
CAAGCACAAAGCGTCCTGTT

Product: hypothetical protein

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase [H]

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MNNSTVSLTATSPLAQWLSYLENSHFKAIDLGLDRIKSVAKALDLLTPAPFVITVGGTNGKGTTCRLLETVLLKAGYRVG
VYSSPHLLRYNERVRIQDQELPDEAHTASFAFIEQHKRESLTYFEFSTLSALHLFKQANLDVVILEVGLGGRLDATNIVD
SDVAVITSIDIDHVDFLGADREQIGFEKAGIFRAHKPAIIGEPNIPQRLLEHAKSLGCQISCRDQDWHFRLQAEHWDWCG
QKVRLKNLPCCQIPLANAATALATFEQLPFQISEEVIRQSLQEVELVGRFQTLKPDVLFPLAENLAKPWADFPQMIIDVG
HNPHAALYLAEKLTALKAKITGKIVAVCGILKDKDAQGVLSPLLPVIDEWHCVTLEGYRGQSGQALFVTLQQIAQGQSHQ
LHANTASSVALGVKQALATTTAHDVILVFGSFHTVGEFLQLL

Sequences:

>Translated_442_residues
MNNSTVSLTATSPLAQWLSYLENSHFKAIDLGLDRIKSVAKALDLLTPAPFVITVGGTNGKGTTCRLLETVLLKAGYRVG
VYSSPHLLRYNERVRIQDQELPDEAHTASFAFIEQHKRESLTYFEFSTLSALHLFKQANLDVVILEVGLGGRLDATNIVD
SDVAVITSIDIDHVDFLGADREQIGFEKAGIFRAHKPAIIGEPNIPQRLLEHAKSLGCQISCRDQDWHFRLQAEHWDWCG
QKVRLKNLPCCQIPLANAATALATFEQLPFQISEEVIRQSLQEVELVGRFQTLKPDVLFPLAENLAKPWADFPQMIIDVG
HNPHAALYLAEKLTALKAKITGKIVAVCGILKDKDAQGVLSPLLPVIDEWHCVTLEGYRGQSGQALFVTLQQIAQGQSHQ
LHANTASSVALGVKQALATTTAHDVILVFGSFHTVGEFLQLL
>Mature_442_residues
MNNSTVSLTATSPLAQWLSYLENSHFKAIDLGLDRIKSVAKALDLLTPAPFVITVGGTNGKGTTCRLLETVLLKAGYRVG
VYSSPHLLRYNERVRIQDQELPDEAHTASFAFIEQHKRESLTYFEFSTLSALHLFKQANLDVVILEVGLGGRLDATNIVD
SDVAVITSIDIDHVDFLGADREQIGFEKAGIFRAHKPAIIGEPNIPQRLLEHAKSLGCQISCRDQDWHFRLQAEHWDWCG
QKVRLKNLPCCQIPLANAATALATFEQLPFQISEEVIRQSLQEVELVGRFQTLKPDVLFPLAENLAKPWADFPQMIIDVG
HNPHAALYLAEKLTALKAKITGKIVAVCGILKDKDAQGVLSPLLPVIDEWHCVTLEGYRGQSGQALFVTLQQIAQGQSHQ
LHANTASSVALGVKQALATTTAHDVILVFGSFHTVGEFLQLL

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=210, Percent_Identity=35.7142857142857, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI66932990, Length=210, Percent_Identity=35.7142857142857, Blast_Score=121, Evalue=1e-27,
Organism=Escherichia coli, GI1788654, Length=434, Percent_Identity=51.6129032258064, Blast_Score=415, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17553148, Length=191, Percent_Identity=36.1256544502618, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71984923, Length=191, Percent_Identity=36.1256544502618, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17553150, Length=191, Percent_Identity=36.1256544502618, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6323760, Length=354, Percent_Identity=28.8135593220339, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6324815, Length=178, Percent_Identity=33.1460674157303, Blast_Score=96, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24641571, Length=205, Percent_Identity=37.5609756097561, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24581568, Length=178, Percent_Identity=35.3932584269663, Blast_Score=93, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 48715; Mature: 48715

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1 ; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNSTVSLTATSPLAQWLSYLENSHFKAIDLGLDRIKSVAKALDLLTPAPFVITVGGTNG
CCCCEEEEEECHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
KGTTCRLLETVLLKAGYRVGVYSSPHLLRYNERVRIQDQELPDEAHTASFAFIEQHKRES
CCHHHHHHHHHHHHCCCEEEECCCCCEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHCC
LTYFEFSTLSALHLFKQANLDVVILEVGLGGRLDATNIVDSDVAVITSIDIDHVDFLGAD
CEEEEHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEECCCHHHHCCCC
REQIGFEKAGIFRAHKPAIIGEPNIPQRLLEHAKSLGCQISCRDQDWHFRLQAEHWDWCG
HHHCCCHHHCCEECCCCCEECCCCCHHHHHHHHHHCCCEEEEECCCEEEEEECCCCCCCC
QKVRLKNLPCCQIPLANAATALATFEQLPFQISEEVIRQSLQEVELVGRFQTLKPDVLFP
CEEEECCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
LAENLAKPWADFPQMIIDVGHNPHAALYLAEKLTALKAKITGKIVAVCGILKDKDAQGVL
HHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHEEEHHHHCCCCCHHHH
SPLLPVIDEWHCVTLEGYRGQSGQALFVTLQQIAQGQSHQLHANTASSVALGVKQALATT
HHHHHHHCCCEEEEEECCCCCCCCCHHEEHHHHHCCCCCEEECCCHHHHHHHHHHHHHHH
TAHDVILVFGSFHTVGEFLQLL
HHCEEEEEECCHHHHHHHHHHC
>Mature Secondary Structure
MNNSTVSLTATSPLAQWLSYLENSHFKAIDLGLDRIKSVAKALDLLTPAPFVITVGGTNG
CCCCEEEEEECHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
KGTTCRLLETVLLKAGYRVGVYSSPHLLRYNERVRIQDQELPDEAHTASFAFIEQHKRES
CCHHHHHHHHHHHHCCCEEEECCCCCEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHCC
LTYFEFSTLSALHLFKQANLDVVILEVGLGGRLDATNIVDSDVAVITSIDIDHVDFLGAD
CEEEEHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEECCCHHHHCCCC
REQIGFEKAGIFRAHKPAIIGEPNIPQRLLEHAKSLGCQISCRDQDWHFRLQAEHWDWCG
HHHCCCHHHCCEECCCCCEECCCCCHHHHHHHHHHCCCEEEEECCCEEEEEECCCCCCCC
QKVRLKNLPCCQIPLANAATALATFEQLPFQISEEVIRQSLQEVELVGRFQTLKPDVLFP
CEEEECCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
LAENLAKPWADFPQMIIDVGHNPHAALYLAEKLTALKAKITGKIVAVCGILKDKDAQGVL
HHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHEEEHHHHCCCCCHHHH
SPLLPVIDEWHCVTLEGYRGQSGQALFVTLQQIAQGQSHQLHANTASSVALGVKQALATT
HHHHHHHCCCEEEEEECCCCCCCCCHHEEHHHHHCCCCCEEECCCHHHHHHHHHHHHHHH
TAHDVILVFGSFHTVGEFLQLL
HHCEEEEEECCHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]