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The map label for this gene is pheS

Identifier: 15602496

GI number: 15602496

Start: 733550

End: 734533

Strand: Reverse

Name: pheS

Synonym: PM0631

Alternate gene names: 15602496

Gene position: 734533-733550 (Counterclockwise)

Preceding gene: 15602497

Following gene: 15602495

Centisome position: 32.54

GC content: 40.96

Gene sequence:

>984_bases
ATGCAAAACCTAGAACAAATTACCGAACAAGCATTGGATGCAATTGCCCGTGCGGAAGATGGCAAAGCATTAGATGCGAT
TCGTGTGGAATATTTTGGTAAAAAGGGACCTTTTACGGCATTAATGCAAGGATTACGTGATGTGTCACCAGAAGCACGTC
CAGCCGTAGGTCAAAAAATTAACGATGCAAAACAACTTGCACAAAATGCATTAAATGAAAAGAAAGTACAGCTTGAAACA
GCGGAATTAAATGCACAATTAGCGAAAGAAAAAATTGATGTAAGCTTGCCGGGACGTAAAGTGGAAACTGGGGGATTACA
TCCAGTGACAATGACGATTGATCGTGTGACCAAATTCTTCTCTGAGTTAGGTTTTTCCGTCGAAAGTGGTCCAGAAATTG
AAAGCGACTATTATAATTTCGATGCCCTCAATATCCCGAAACATCACCCAGCCCGAGCTGATCACGATACATTTTGGTTT
AACCCAGAATTATTACTACGCACACAAACCTCTGGTGTACAAATTCGTACGATGGAAAAAATGCAACCGCCAATTCGCAT
TATGGCACCAGGACGTGTTTACCGTAACGATTATGATCAAACACATACACCGATGTTCCACCAAATTGAATTATTGTATG
TGGATAAAAAAGCGAACTTCACTGAATTAAAAGGGTTACTCCATGACTTCTTACGCGCCTTTTTTGAAGAAGACTTACAA
GTGCGTTTCCGTCCTTCCTATTTCCCGTTCACTGAACCTTCCGCTGAAGTGGATGTGATGGGCAAAAATGGCAAATGGTT
AGAAGTACTAGGTTGTGGTATGGTACATCCGAATGTTTTACGCAATGTTGGGATTGACCCAAATGAATATGCTGGCTTTG
CGGTGGGGATGGGGGTTGAGCGCTTAACGATGTTACGCTACAACGTCACAGATTTACGTGCTTTCTTTGAAAATGACTTA
CGTTTCTTAAAACAATTTAAATAA

Upstream 100 bases:

>100_bases
CATTAAAACACAAAAAACGCCAATAAAAACGTGATTTTTAAGGCAGTTAGTATTATCATTAACAGTATTTTTCACAGCAT
AATTGAATTTAGAGGAAGGC

Downstream 100 bases:

>100_bases
GAGAATCGCCATGAAAAAAATCATAGCAATGAGTTTGCTTATGTTTTCTGTTGTGATGTCAGTAAATGCAAAAAATACGG
TTGCCAGTAAAAAAGAAGAA

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MQNLEQITEQALDAIARAEDGKALDAIRVEYFGKKGPFTALMQGLRDVSPEARPAVGQKINDAKQLAQNALNEKKVQLET
AELNAQLAKEKIDVSLPGRKVETGGLHPVTMTIDRVTKFFSELGFSVESGPEIESDYYNFDALNIPKHHPARADHDTFWF
NPELLLRTQTSGVQIRTMEKMQPPIRIMAPGRVYRNDYDQTHTPMFHQIELLYVDKKANFTELKGLLHDFLRAFFEEDLQ
VRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVHPNVLRNVGIDPNEYAGFAVGMGVERLTMLRYNVTDLRAFFENDL
RFLKQFK

Sequences:

>Translated_327_residues
MQNLEQITEQALDAIARAEDGKALDAIRVEYFGKKGPFTALMQGLRDVSPEARPAVGQKINDAKQLAQNALNEKKVQLET
AELNAQLAKEKIDVSLPGRKVETGGLHPVTMTIDRVTKFFSELGFSVESGPEIESDYYNFDALNIPKHHPARADHDTFWF
NPELLLRTQTSGVQIRTMEKMQPPIRIMAPGRVYRNDYDQTHTPMFHQIELLYVDKKANFTELKGLLHDFLRAFFEEDLQ
VRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVHPNVLRNVGIDPNEYAGFAVGMGVERLTMLRYNVTDLRAFFENDL
RFLKQFK
>Mature_327_residues
MQNLEQITEQALDAIARAEDGKALDAIRVEYFGKKGPFTALMQGLRDVSPEARPAVGQKINDAKQLAQNALNEKKVQLET
AELNAQLAKEKIDVSLPGRKVETGGLHPVTMTIDRVTKFFSELGFSVESGPEIESDYYNFDALNIPKHHPARADHDTFWF
NPELLLRTQTSGVQIRTMEKMQPPIRIMAPGRVYRNDYDQTHTPMFHQIELLYVDKKANFTELKGLLHDFLRAFFEEDLQ
VRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVHPNVLRNVGIDPNEYAGFAVGMGVERLTMLRYNVTDLRAFFENDL
RFLKQFK

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5729820, Length=274, Percent_Identity=30.2919708029197, Blast_Score=121, Evalue=8e-28,
Organism=Homo sapiens, GI4758340, Length=259, Percent_Identity=30.8880308880309, Blast_Score=116, Evalue=3e-26,
Organism=Escherichia coli, GI1788007, Length=327, Percent_Identity=74.0061162079511, Blast_Score=514, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI32566635, Length=242, Percent_Identity=33.8842975206612, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17508957, Length=260, Percent_Identity=29.2307692307692, Blast_Score=114, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI32563657, Length=260, Percent_Identity=28.8461538461538, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6321087, Length=267, Percent_Identity=28.8389513108614, Blast_Score=117, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6325304, Length=90, Percent_Identity=41.1111111111111, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI18858079, Length=258, Percent_Identity=29.8449612403101, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI17137424, Length=280, Percent_Identity=28.2142857142857, Blast_Score=108, Evalue=4e-24,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_PASMU (P57860)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245568.1
- ProteinModelPortal:   P57860
- SMR:   P57860
- GeneID:   1243978
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0631
- NMPDR:   fig|272843.1.peg.631
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- ProtClustDB:   PRK00488
- BioCyc:   PMUL272843:PM0631-MONOMER
- BRENDA:   6.1.1.20
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 37314; Mature: 37314

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNLEQITEQALDAIARAEDGKALDAIRVEYFGKKGPFTALMQGLRDVSPEARPAVGQKI
CCHHHHHHHHHHHHHHHCCCCCHHHHEEHHHCCCCCCHHHHHHHHHHCCCCCCCHHCCCH
NDAKQLAQNALNEKKVQLETAELNAQLAKEKIDVSLPGRKVETGGLHPVTMTIDRVTKFF
HHHHHHHHHHHCHHHEEEEHHHHHHHHHHHHCCEECCCCEECCCCCCEEEEEHHHHHHHH
SELGFSVESGPEIESDYYNFDALNIPKHHPARADHDTFWFNPELLLRTQTSGVQIRTMEK
HHHCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCEEEECCEEEEEECCCCEEEEEHHH
MQPPIRIMAPGRVYRNDYDQTHTPMFHQIELLYVDKKANFTELKGLLHDFLRAFFEEDLQ
CCCCEEEECCCHHHCCCCCCCCCCHHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCE
VRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVHPNVLRNVGIDPNEYAGFAVGMGVE
EEECCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCHHHHHHCCCCCCCCCCEEECCCHH
RLTMLRYNVTDLRAFFENDLRFLKQFK
HHHHHHCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQNLEQITEQALDAIARAEDGKALDAIRVEYFGKKGPFTALMQGLRDVSPEARPAVGQKI
CCHHHHHHHHHHHHHHHCCCCCHHHHEEHHHCCCCCCHHHHHHHHHHCCCCCCCHHCCCH
NDAKQLAQNALNEKKVQLETAELNAQLAKEKIDVSLPGRKVETGGLHPVTMTIDRVTKFF
HHHHHHHHHHHCHHHEEEEHHHHHHHHHHHHCCEECCCCEECCCCCCEEEEEHHHHHHHH
SELGFSVESGPEIESDYYNFDALNIPKHHPARADHDTFWFNPELLLRTQTSGVQIRTMEK
HHHCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCEEEECCEEEEEECCCCEEEEEHHH
MQPPIRIMAPGRVYRNDYDQTHTPMFHQIELLYVDKKANFTELKGLLHDFLRAFFEEDLQ
CCCCEEEECCCHHHCCCCCCCCCCHHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCE
VRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVHPNVLRNVGIDPNEYAGFAVGMGVE
EEECCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCHHHHHHCCCCCCCCCCEEECCCHH
RLTMLRYNVTDLRAFFENDLRFLKQFK
HHHHHHCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100