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The map label for this gene is mdh_2 [H]

Identifier: 15602415

GI number: 15602415

Start: 634091

End: 635026

Strand: Direct

Name: mdh_2 [H]

Synonym: PM0550

Alternate gene names: 15602415

Gene position: 634091-635026 (Clockwise)

Preceding gene: 15602410

Following gene: 15602417

Centisome position: 28.09

GC content: 41.77

Gene sequence:

>936_bases
ATGAAAGTTGCAGTTCTAGGTGCCGCAGGTGGTATTGGTCAAGCGTTAGCATTATTATTGAAATTACAATTACCAGCCGG
TTCAGAGTTATCATTATATGATATTGCCCCCGTGACACCAGGTGTTGCTGCGGATGTCAGCCATATTCCAACAGCTGTAA
AAGTGCAAGGATTTGCCGGTGAAGATCCAACACCTGCATTACAAGGGGCTGATGTAGTATTAATTTCTGCAGGTGTTGCA
CGTAAACCGGGTATGGATCGTTCTGATTTATTTAATATTAATGCCGGTATCGTGCGTAACTTAATTGAGAAAGTTGCGCA
AGTTTGTCCAAAAGCTTGTGTGGGGATTATTACTAATCCTGTGAATACGACAGTAGCAATTGCGGCTGAAGTATTGAAAA
AAGCGGGTGTTTACGATAAGCGTAAATTATTCGGTGTAACCACATTAGATATTATTCGTTCTGAAACCTTTGTATCAGAA
TTGAAAAATTTAGAGCCAGCGCGCACGACAGTGCCTGTGATTGGTGGGCATTCTGGTGTCACCATTCTTCCATTACTTTC
TCAAGTCCAATACGCAGAGTGGAATGACGCGGAAATTGCACCATTAACAAAACGTATTCAAAATGCGGGTACCGAAGTGG
TCGAAGCAAAAGCGGGTGGTGGTTCAGCAACGTTATCCATGGCACAAGCCGCAGCACGTTTTGCACTTTCTTTAGTTAAA
GGCTTAAATGGGGAAAATGTAGTTGAATGTACTTATGTGGAAGGTGATGGCAAATATGCCCGTTTCTTTGCTCAGCCTGT
TCGTTTAGGTAAAGAAGGCGTTGAAGAAATTCTACCATTAGGGTCATTGAGTGCATTTGAACAACATGCATTAGATGCAA
TGTTAGAGACATTACGTGCGGATATTGAATTAGGCGAGAAATTTGTCAATCAATAA

Upstream 100 bases:

>100_bases
TTTGAGTTAGATCTCACTATTGCATTTCTTTATTTGAAATTGGTTGTGGTTCATTCTAAAATCATAGGTATTTAGTTTAT
TAACCCATAAGGAGTAGCAA

Downstream 100 bases:

>100_bases
TCTGTGACAGGAAAAAGTGATTTCTAAAGAGTATCAAGATTTATTGATACTCTTTTTTTATCACAGCTAAGCCGAAATCA
TTTTTACTTTTTTATCTTAA

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MKVAVLGAAGGIGQALALLLKLQLPAGSELSLYDIAPVTPGVAADVSHIPTAVKVQGFAGEDPTPALQGADVVLISAGVA
RKPGMDRSDLFNINAGIVRNLIEKVAQVCPKACVGIITNPVNTTVAIAAEVLKKAGVYDKRKLFGVTTLDIIRSETFVSE
LKNLEPARTTVPVIGGHSGVTILPLLSQVQYAEWNDAEIAPLTKRIQNAGTEVVEAKAGGGSATLSMAQAAARFALSLVK
GLNGENVVECTYVEGDGKYARFFAQPVRLGKEGVEEILPLGSLSAFEQHALDAMLETLRADIELGEKFVNQ

Sequences:

>Translated_311_residues
MKVAVLGAAGGIGQALALLLKLQLPAGSELSLYDIAPVTPGVAADVSHIPTAVKVQGFAGEDPTPALQGADVVLISAGVA
RKPGMDRSDLFNINAGIVRNLIEKVAQVCPKACVGIITNPVNTTVAIAAEVLKKAGVYDKRKLFGVTTLDIIRSETFVSE
LKNLEPARTTVPVIGGHSGVTILPLLSQVQYAEWNDAEIAPLTKRIQNAGTEVVEAKAGGGSATLSMAQAAARFALSLVK
GLNGENVVECTYVEGDGKYARFFAQPVRLGKEGVEEILPLGSLSAFEQHALDAMLETLRADIELGEKFVNQ
>Mature_311_residues
MKVAVLGAAGGIGQALALLLKLQLPAGSELSLYDIAPVTPGVAADVSHIPTAVKVQGFAGEDPTPALQGADVVLISAGVA
RKPGMDRSDLFNINAGIVRNLIEKVAQVCPKACVGIITNPVNTTVAIAAEVLKKAGVYDKRKLFGVTTLDIIRSETFVSE
LKNLEPARTTVPVIGGHSGVTILPLLSQVQYAEWNDAEIAPLTKRIQNAGTEVVEAKAGGGSATLSMAQAAARFALSLVK
GLNGENVVECTYVEGDGKYARFFAQPVRLGKEGVEEILPLGSLSAFEQHALDAMLETLRADIELGEKFVNQ

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 1 family [H]

Homologues:

Organism=Homo sapiens, GI21735621, Length=311, Percent_Identity=56.2700964630225, Blast_Score=326, Evalue=2e-89,
Organism=Escherichia coli, GI1789632, Length=312, Percent_Identity=79.4871794871795, Blast_Score=501, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI17554310, Length=311, Percent_Identity=57.8778135048232, Blast_Score=335, Evalue=2e-92,
Organism=Saccharomyces cerevisiae, GI6322765, Length=315, Percent_Identity=51.4285714285714, Blast_Score=281, Evalue=8e-77,
Organism=Saccharomyces cerevisiae, GI6320125, Length=338, Percent_Identity=40.5325443786982, Blast_Score=196, Evalue=6e-51,
Organism=Saccharomyces cerevisiae, GI6324446, Length=279, Percent_Identity=43.010752688172, Blast_Score=184, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24647881, Length=312, Percent_Identity=58.0128205128205, Blast_Score=332, Evalue=2e-91,
Organism=Drosophila melanogaster, GI24663599, Length=313, Percent_Identity=50.1597444089457, Blast_Score=276, Evalue=1e-74,
Organism=Drosophila melanogaster, GI24663595, Length=298, Percent_Identity=45.9731543624161, Blast_Score=251, Evalue=3e-67,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR001252
- InterPro:   IPR010097
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 32549; Mature: 32549

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00068 MDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAVLGAAGGIGQALALLLKLQLPAGSELSLYDIAPVTPGVAADVSHIPTAVKVQGFAG
CEEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHCCCEEEEECCCC
EDPTPALQGADVVLISAGVARKPGMDRSDLFNINAGIVRNLIEKVAQVCPKACVGIITNP
CCCCCCCCCCCEEEEECCCCCCCCCCHHHEEECCHHHHHHHHHHHHHHHHHHHHHHHCCC
VNTTVAIAAEVLKKAGVYDKRKLFGVTTLDIIRSETFVSELKNLEPARTTVPVIGGHSGV
CHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
TILPLLSQVQYAEWNDAEIAPLTKRIQNAGTEVVEAKAGGGSATLSMAQAAARFALSLVK
HHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHH
GLNGENVVECTYVEGDGKYARFFAQPVRLGKEGVEEILPLGSLSAFEQHALDAMLETLRA
CCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
DIELGEKFVNQ
HHHHHHHHHCC
>Mature Secondary Structure
MKVAVLGAAGGIGQALALLLKLQLPAGSELSLYDIAPVTPGVAADVSHIPTAVKVQGFAG
CEEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHCCCEEEEECCCC
EDPTPALQGADVVLISAGVARKPGMDRSDLFNINAGIVRNLIEKVAQVCPKACVGIITNP
CCCCCCCCCCCEEEEECCCCCCCCCCHHHEEECCHHHHHHHHHHHHHHHHHHHHHHHCCC
VNTTVAIAAEVLKKAGVYDKRKLFGVTTLDIIRSETFVSELKNLEPARTTVPVIGGHSGV
CHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
TILPLLSQVQYAEWNDAEIAPLTKRIQNAGTEVVEAKAGGGSATLSMAQAAARFALSLVK
HHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHH
GLNGENVVECTYVEGDGKYARFFAQPVRLGKEGVEEILPLGSLSAFEQHALDAMLETLRA
CCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
DIELGEKFVNQ
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA