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The map label for this gene is thiI

Identifier: 15602400

GI number: 15602400

Start: 611759

End: 613201

Strand: Direct

Name: thiI

Synonym: PM0535

Alternate gene names: 15602400

Gene position: 611759-613201 (Clockwise)

Preceding gene: 15602396

Following gene: 15602402

Centisome position: 27.1

GC content: 39.29

Gene sequence:

>1443_bases
ATGAAATTTGTGATTAAACTGTTTCCCGAAATTATGATTAAAAGTGAATCGGTACGTAAACGATTCGTCAAGATCTTAAC
TGGGAATATTCGTAATATTTTAACAAAACATGATGAAACTATTGCGGTGGTTCGTCATTGGGATTACATCGAAGTTCGTT
CGAAAAAAGAAGAAAATCGACCGCACTTAATTGAATTATTAGGGCGTATTCCGGGCATTCATCACTTTTTAGAAGTAGAT
GAAAAGCCGTTTACAACGATACACGATATTTTTGAACAGACTTTGCAAGATATTGGAACAAGTCTTGAGAACAAAACATT
TTGTGTACGGGTTAGACGAAAAGGCAAGCATACGTTTAATTCTTTGGATGTTGAACGTTATGTCGGTGGTGGCTTAAATC
AACATATTCCGAGTGCCAAAGTACAACTGAGTAAACCAGATGTCACCGTGCGTATTGATATTGAAAATGACAACATGATG
TTGATTAAAGCACGCCATGTTGGCATTGGCGGTTATCCGATTGGGACGCAAGAAGATGTATTATCACTGATTTCAGGTGG
CTTTGATTCCGGTGTATCAAGTTATATGCTGATTCGACGTGGTTCACGTGTGCATTATTGCTTCTTTAATCTTGGCGGTG
CGGCACATGAAATTGGTGTGAAACAAATGGCATACCATATTTGGCAACGTTATAGTGCATCACACAAAGTCCGTTTTGTG
GCAATCAATTTTGAAGGCGTGGTCGGCGAAATTTTGGAAAAAGTCGATAACGGGCAGATGGGCGTTGTCTTAAAACGTAT
GATGGTGCGAGCGGCGAGCCGTATTGCCGAGCGTTTTGGGATTCAAGCGATCGTGACAGGCGAAGCACTTGGTCAAGTGT
CTAGTCAAACCCTCACCAATTTACGCTTAATTGATGAGGCATCAGAGAGCTTAGTACTACGCCCATTAATCACCCACGAT
AAAGAACAGATTATCGCAATGGCGAAAGAAATTGGAACGGATGATATTGCTAAATCGATGCCCGAGTTTTGTGGTGTGAT
TTCGAAAAATCCAACGGTGAAAGCAATTAAAGCGAAGATTGAGCAAGAAGAACGTCATTTTGATTTTGCTGTATTAGAAA
GTGCGGTGCAAAATGCACAATATTTAGATATTCGTCAGATTGCCGAACAGACTGAAAAAGAAGTAGTTGCAGTAGATACG
GTCGCTGTTTTGAGTGCGCAAGATGTAATTTTGGATATTCGTAGTCCAGAAGAAACGGATGAAAAGCCACTCAATATGGA
AAATGTGCAATTAATGCCATTTTATAAATTATCTTCACAATTTGCCAATTTGGATCAGAGTAAGAATTACCTTTTATATT
GTGAGCGTGGTGTAATGAGCAAATTACAAGCCCTATATCTAAAAGAACAAGGATTTTCTAATGTGAAGGTTTTCCGTCAA
TAG

Upstream 100 bases:

>100_bases
ATTTGTTGGTTTTAAGCGTAAATTGTGAAAGATTTTTGATTCTCGGGCAGATCTGATAGAATGCACCGCTTGTAACTTGA
AGACAGAAAATAAATAAACT

Downstream 100 bases:

>100_bases
GGGAAAAAGATGCCGAGCAGTTTACTCGGCATTTTTCACTTATTTTTTCCAAACGACGTGGAATTCACGATCTTCTTCAC
GATGAGAAATTAAGAATTTA

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MKFVIKLFPEIMIKSESVRKRFVKILTGNIRNILTKHDETIAVVRHWDYIEVRSKKEENRPHLIELLGRIPGIHHFLEVD
EKPFTTIHDIFEQTLQDIGTSLENKTFCVRVRRKGKHTFNSLDVERYVGGGLNQHIPSAKVQLSKPDVTVRIDIENDNMM
LIKARHVGIGGYPIGTQEDVLSLISGGFDSGVSSYMLIRRGSRVHYCFFNLGGAAHEIGVKQMAYHIWQRYSASHKVRFV
AINFEGVVGEILEKVDNGQMGVVLKRMMVRAASRIAERFGIQAIVTGEALGQVSSQTLTNLRLIDEASESLVLRPLITHD
KEQIIAMAKEIGTDDIAKSMPEFCGVISKNPTVKAIKAKIEQEERHFDFAVLESAVQNAQYLDIRQIAEQTEKEVVAVDT
VAVLSAQDVILDIRSPEETDEKPLNMENVQLMPFYKLSSQFANLDQSKNYLLYCERGVMSKLQALYLKEQGFSNVKVFRQ

Sequences:

>Translated_480_residues
MKFVIKLFPEIMIKSESVRKRFVKILTGNIRNILTKHDETIAVVRHWDYIEVRSKKEENRPHLIELLGRIPGIHHFLEVD
EKPFTTIHDIFEQTLQDIGTSLENKTFCVRVRRKGKHTFNSLDVERYVGGGLNQHIPSAKVQLSKPDVTVRIDIENDNMM
LIKARHVGIGGYPIGTQEDVLSLISGGFDSGVSSYMLIRRGSRVHYCFFNLGGAAHEIGVKQMAYHIWQRYSASHKVRFV
AINFEGVVGEILEKVDNGQMGVVLKRMMVRAASRIAERFGIQAIVTGEALGQVSSQTLTNLRLIDEASESLVLRPLITHD
KEQIIAMAKEIGTDDIAKSMPEFCGVISKNPTVKAIKAKIEQEERHFDFAVLESAVQNAQYLDIRQIAEQTEKEVVAVDT
VAVLSAQDVILDIRSPEETDEKPLNMENVQLMPFYKLSSQFANLDQSKNYLLYCERGVMSKLQALYLKEQGFSNVKVFRQ
>Mature_480_residues
MKFVIKLFPEIMIKSESVRKRFVKILTGNIRNILTKHDETIAVVRHWDYIEVRSKKEENRPHLIELLGRIPGIHHFLEVD
EKPFTTIHDIFEQTLQDIGTSLENKTFCVRVRRKGKHTFNSLDVERYVGGGLNQHIPSAKVQLSKPDVTVRIDIENDNMM
LIKARHVGIGGYPIGTQEDVLSLISGGFDSGVSSYMLIRRGSRVHYCFFNLGGAAHEIGVKQMAYHIWQRYSASHKVRFV
AINFEGVVGEILEKVDNGQMGVVLKRMMVRAASRIAERFGIQAIVTGEALGQVSSQTLTNLRLIDEASESLVLRPLITHD
KEQIIAMAKEIGTDDIAKSMPEFCGVISKNPTVKAIKAKIEQEERHFDFAVLESAVQNAQYLDIRQIAEQTEKEVVAVDT
VAVLSAQDVILDIRSPEETDEKPLNMENVQLMPFYKLSSQFANLDQSKNYLLYCERGVMSKLQALYLKEQGFSNVKVFRQ

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1786625, Length=481, Percent_Identity=68.8149688149688, Blast_Score=693, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THII_PASMU (P57849)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245472.1
- ProteinModelPortal:   P57849
- PRIDE:   P57849
- GeneID:   1243882
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0535
- NMPDR:   fig|272843.1.peg.535
- HOGENOM:   HBG646072
- OMA:   GYPIGTQ
- ProtClustDB:   PRK01269
- BioCyc:   PMUL272843:PM0535-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00021
- InterPro:   IPR001763
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114
- Gene3D:   G3DSA:3.40.250.10
- Gene3D:   G3DSA:3.40.50.620
- SMART:   SM00981
- TIGRFAMs:   TIGR00342

Pfam domain/function: PF02568 ThiI; PF02926 THUMP; SSF52821 Rhodanese-like

EC number: =2.8.1.4

Molecular weight: Translated: 54429; Mature: 54429

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: PS50206 RHODANESE_3; PS51165 THUMP

Important sites: ACT_SITE 454-454 BINDING 265-265 BINDING 287-287 BINDING 296-296

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFVIKLFPEIMIKSESVRKRFVKILTGNIRNILTKHDETIAVVRHWDYIEVRSKKEENR
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEECCCCCCCC
PHLIELLGRIPGIHHFLEVDEKPFTTIHDIFEQTLQDIGTSLENKTFCVRVRRKGKHTFN
CHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCC
SLDVERYVGGGLNQHIPSAKVQLSKPDVTVRIDIENDNMMLIKARHVGIGGYPIGTQEDV
CCCHHHHHCCCCCCCCCCCEEEECCCCEEEEEEECCCCEEEEEEEECCCCCCCCCCHHHH
LSLISGGFDSGVSSYMLIRRGSRVHYCFFNLGGAAHEIGVKQMAYHIWQRYSASHKVRFV
HHHHHCCCCCCHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEE
AINFEGVVGEILEKVDNGQMGVVLKRMMVRAASRIAERFGIQAIVTGEALGQVSSQTLTN
EEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHHH
LRLIDEASESLVLRPLITHDKEQIIAMAKEIGTDDIAKSMPEFCGVISKNPTVKAIKAKI
HHHHHHCCCCEEEEHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHH
EQEERHFDFAVLESAVQNAQYLDIRQIAEQTEKEVVAVDTVAVLSAQDVILDIRSPEETD
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCEEEEHHHHHHHHCEEEEECCCCCCCC
EKPLNMENVQLMPFYKLSSQFANLDQSKNYLLYCERGVMSKLQALYLKEQGFSNVKVFRQ
CCCCCCCCEEEEEHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCCEEECC
>Mature Secondary Structure
MKFVIKLFPEIMIKSESVRKRFVKILTGNIRNILTKHDETIAVVRHWDYIEVRSKKEENR
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEECCCCCCCC
PHLIELLGRIPGIHHFLEVDEKPFTTIHDIFEQTLQDIGTSLENKTFCVRVRRKGKHTFN
CHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCC
SLDVERYVGGGLNQHIPSAKVQLSKPDVTVRIDIENDNMMLIKARHVGIGGYPIGTQEDV
CCCHHHHHCCCCCCCCCCCEEEECCCCEEEEEEECCCCEEEEEEEECCCCCCCCCCHHHH
LSLISGGFDSGVSSYMLIRRGSRVHYCFFNLGGAAHEIGVKQMAYHIWQRYSASHKVRFV
HHHHHCCCCCCHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEE
AINFEGVVGEILEKVDNGQMGVVLKRMMVRAASRIAERFGIQAIVTGEALGQVSSQTLTN
EEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHHH
LRLIDEASESLVLRPLITHDKEQIIAMAKEIGTDDIAKSMPEFCGVISKNPTVKAIKAKI
HHHHHHCCCCEEEEHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHH
EQEERHFDFAVLESAVQNAQYLDIRQIAEQTEKEVVAVDTVAVLSAQDVILDIRSPEETD
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCEEEEHHHHHHHHCEEEEECCCCCCCC
EKPLNMENVQLMPFYKLSSQFANLDQSKNYLLYCERGVMSKLQALYLKEQGFSNVKVFRQ
CCCCCCCCEEEEEHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100