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The map label for this gene is truC

Identifier: 15602344

GI number: 15602344

Start: 562927

End: 563658

Strand: Direct

Name: truC

Synonym: PM0479

Alternate gene names: 15602344

Gene position: 562927-563658 (Clockwise)

Preceding gene: 15602343

Following gene: 15602345

Centisome position: 24.94

GC content: 39.34

Gene sequence:

>732_bases
ATGACATTAGAGATTTTATATCAAGATCCATATTTGATTGCTGTAAATAAACCAGCAGGTATGCTAGTCCATCGTAGTTG
GTTAGATCGGCATGAAACACAATTTGTCATGCAAACATTACGCGATCAAATAGGGCAGCATGTTTATCCTATTCACCGTT
TAGATCGACCGACTTCAGGTGTTTTACTTTTCGCATTAAGTAGCGAAGTGGCAAATTTATTGTGTCTTCAATTTGAAAAT
AAGCAAGTGCAAAAAAGTTATTTAGCGATTGTTCGAGGATATTTAGTTGGTGAGGGGCGAATTGATTACCCACTGAAAGT
TCAATTAGACAAAATTGCGGATAAGTTTGCCCAAGATGACAAGGCGCCACAAGAAGCGGTGACAGATTATCTCGGGTTAG
CTAAAGTTGAAATGCCGTATTGTGTGAAAAAGTACCCAACAACGCGTTATTCGCTTGTGCGTTTAATTCCGCATACGGGG
CGAAAACATCAGCTGCGTCGTCACATGAAACACCTTTTTCATCCTATTTTGGGAGATACACAATATGGTGATTTACACCA
AAATCGTGCATTAACCACTCATACGGGCGTGCAACGTTTAATGTTACATGCAGAACGATTGATCTTTACACACCCGATAA
CACAGCAACAGATCACGATCTGTGCGGGTGTCGATCAACAATGGCTCGATTTGATTAATATTTTTGATTGGGATTTAAAT
TCCATACATTAA

Upstream 100 bases:

>100_bases
AATTACGCCTTATCTTGAGGAGGCGCTGAAAGAAATGGATGGATTGGCTGATTTATTAAGTCCGATCAGCGAAATTGAAA
AATTGCTGCAGAAATAAGTC

Downstream 100 bases:

>100_bases
GTATGGGTAACTGAATTTGTTTTTTCTAGGTGAAATGAAATGTTAGATAATGCACTATTAGGTTTAACGCATGAGCAACA
ACAAGAGGCAGTTGAGAAAA

Product: tRNA pseudouridine synthase C

Products: NA

Alternate protein names: tRNA pseudouridylate synthase C; tRNA-uridine isomerase C

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MTLEILYQDPYLIAVNKPAGMLVHRSWLDRHETQFVMQTLRDQIGQHVYPIHRLDRPTSGVLLFALSSEVANLLCLQFEN
KQVQKSYLAIVRGYLVGEGRIDYPLKVQLDKIADKFAQDDKAPQEAVTDYLGLAKVEMPYCVKKYPTTRYSLVRLIPHTG
RKHQLRRHMKHLFHPILGDTQYGDLHQNRALTTHTGVQRLMLHAERLIFTHPITQQQITICAGVDQQWLDLINIFDWDLN
SIH

Sequences:

>Translated_243_residues
MTLEILYQDPYLIAVNKPAGMLVHRSWLDRHETQFVMQTLRDQIGQHVYPIHRLDRPTSGVLLFALSSEVANLLCLQFEN
KQVQKSYLAIVRGYLVGEGRIDYPLKVQLDKIADKFAQDDKAPQEAVTDYLGLAKVEMPYCVKKYPTTRYSLVRLIPHTG
RKHQLRRHMKHLFHPILGDTQYGDLHQNRALTTHTGVQRLMLHAERLIFTHPITQQQITICAGVDQQWLDLINIFDWDLN
SIH
>Mature_242_residues
TLEILYQDPYLIAVNKPAGMLVHRSWLDRHETQFVMQTLRDQIGQHVYPIHRLDRPTSGVLLFALSSEVANLLCLQFENK
QVQKSYLAIVRGYLVGEGRIDYPLKVQLDKIADKFAQDDKAPQEAVTDYLGLAKVEMPYCVKKYPTTRYSLVRLIPHTGR
KHQLRRHMKHLFHPILGDTQYGDLHQNRALTTHTGVQRLMLHAERLIFTHPITQQQITICAGVDQQWLDLINIFDWDLNS
IH

Specific function: Responsible for synthesis of pseudouridine from uracil- 65 in transfer RNAs

COG id: COG0564

COG function: function code J; Pseudouridylate synthases, 23S RNA-specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rluA family

Homologues:

Organism=Homo sapiens, GI23308689, Length=184, Percent_Identity=30.4347826086957, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI221316667, Length=190, Percent_Identity=33.6842105263158, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI221316663, Length=208, Percent_Identity=31.25, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI1789155, Length=235, Percent_Identity=64.2553191489362, Blast_Score=315, Evalue=2e-87,
Organism=Escherichia coli, GI1787327, Length=224, Percent_Identity=35.2678571428571, Blast_Score=107, Evalue=5e-25,
Organism=Escherichia coli, GI1786244, Length=228, Percent_Identity=36.8421052631579, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI1788946, Length=257, Percent_Identity=26.0700389105058, Blast_Score=83, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324506, Length=188, Percent_Identity=27.6595744680851, Blast_Score=80, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6320168, Length=185, Percent_Identity=28.6486486486486, Blast_Score=79, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6321608, Length=197, Percent_Identity=27.9187817258883, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24583449, Length=197, Percent_Identity=32.48730964467, Blast_Score=78, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24583447, Length=197, Percent_Identity=32.48730964467, Blast_Score=78, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24583445, Length=197, Percent_Identity=32.48730964467, Blast_Score=78, Evalue=4e-15,
Organism=Drosophila melanogaster, GI19921090, Length=181, Percent_Identity=31.4917127071823, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUC_PASMU (Q9CNF3)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245416.1
- ProteinModelPortal:   Q9CNF3
- SMR:   Q9CNF3
- GeneID:   1243826
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0479
- NMPDR:   fig|272843.1.peg.479
- HOGENOM:   HBG750656
- OMA:   KSSEMAN
- ProtClustDB:   PRK11112
- BioCyc:   PMUL272843:PM0479-MONOMER
- InterPro:   IPR020103
- InterPro:   IPR006224
- InterPro:   IPR006145

Pfam domain/function: PF00849 PseudoU_synth_2; SSF55120 PsdUridine_synth_cat_dom

EC number: NA

Molecular weight: Translated: 28242; Mature: 28111

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: PS01129 PSI_RLU

Important sites: ACT_SITE 55-55

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLEILYQDPYLIAVNKPAGMLVHRSWLDRHETQFVMQTLRDQIGQHVYPIHRLDRPTSG
CEEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCC
VLLFALSSEVANLLCLQFENKQVQKSYLAIVRGYLVGEGRIDYPLKVQLDKIADKFAQDD
EEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEECCCCCCCCEEHHHHHHHHHHCCC
KAPQEAVTDYLGLAKVEMPYCVKKYPTTRYSLVRLIPHTGRKHQLRRHMKHLFHPILGDT
CCHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCC
QYGDLHQNRALTTHTGVQRLMLHAERLIFTHPITQQQITICAGVDQQWLDLINIFDWDLN
CCCCHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEECCCHHHHHHHHHHCCCCC
SIH
CCC
>Mature Secondary Structure 
TLEILYQDPYLIAVNKPAGMLVHRSWLDRHETQFVMQTLRDQIGQHVYPIHRLDRPTSG
EEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCC
VLLFALSSEVANLLCLQFENKQVQKSYLAIVRGYLVGEGRIDYPLKVQLDKIADKFAQDD
EEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEECCCCCCCCEEHHHHHHHHHHCCC
KAPQEAVTDYLGLAKVEMPYCVKKYPTTRYSLVRLIPHTGRKHQLRRHMKHLFHPILGDT
CCHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCC
QYGDLHQNRALTTHTGVQRLMLHAERLIFTHPITQQQITICAGVDQQWLDLINIFDWDLN
CCCCHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEECCCHHHHHHHHHHCCCCC
SIH
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100