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The map label for this gene is queF

Identifier: 15602341

GI number: 15602341

Start: 560807

End: 561646

Strand: Reverse

Name: queF

Synonym: PM0476

Alternate gene names: 15602341

Gene position: 561646-560807 (Counterclockwise)

Preceding gene: 15602342

Following gene: 15602340

Centisome position: 24.88

GC content: 39.4

Gene sequence:

>840_bases
ATGCAGTATCAACACGATAGCTTAGACAAACTCAAACTGGGTCAACAAACACAATATGCTTCAAATTATGACCACACTTT
ATTACAACCCGTCCCACGTCACTTAAATCGGGATACCCTCGGCATCACGCATACACAGCCCTTTCATTTTGGTGCCGATA
TTTGGACGGCTTATGAAATTTCTTGGCTAAACTTAAATGGGCTCCCACAAGTCGCCATTGCCGATGTCGCAATTGATTTC
CAGAGTGAAAACTTAATCGAATCAAAAAGCTTTAAATTGTATTTAAACAGTTTCAACCAAAGCAAATTTGCCACATTCGA
GGAAGTACAACAGCATTTAACGCAAGATCTCAGCAATTGTGCTAAAGGGAAAGTCAGCGTCAAATTGCACCCATTGTCAA
AATATTGTCATGAACCCATTGTAGAATTAGCCGGTGAATGTATCGATCAACAAGACATTGAAATTAACGACTATCAGTTT
AATCCCGAGATCTTAACAAATTGCACTCATGATCAAATGGTGAAAGAATCACTCGTCAGCCACCTACTGAAATCCAACTG
TTTAATTACCAATCAACCGGATTGGGGCACACTCCAAATTCGTTATGAAGGCAAACAAATTGATCGTGAGAAACTATTAC
GTTACATTATTTCTTTCCGTCAACATAATGAATTTCATGAACAATGCGTTGAACGGATTTTTTGTGATCTCATGCAATTT
GCTAAACCAGACAAGCTCACTGTTTATGCACGTTATACTCGACGAGGGGGATTAGATATCAATCCTTTCCGTTCTAACTT
TGAAGCGGTTCCAGACAATCAGCGTTTGGCTCGTCAATAG

Upstream 100 bases:

>100_bases
TATTTTAAATGTGATGATTGTCGCCTTGTTTCCAATTTATCATGGCATTGGCAATGAATTAAATGATGAGCGCTCAATTA
TTCGTATGAAGGAAGGTAAG

Downstream 100 bases:

>100_bases
GAGAAATTATGGATAAAAATATTTATTATGTGAATCCACGCGGAAGCATGGATCAGCTCTCTCATTTAGAGGTCGAGCTA
CTCACCAAAAAAGCCAAAAG

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MQYQHDSLDKLKLGQQTQYASNYDHTLLQPVPRHLNRDTLGITHTQPFHFGADIWTAYEISWLNLNGLPQVAIADVAIDF
QSENLIESKSFKLYLNSFNQSKFATFEEVQQHLTQDLSNCAKGKVSVKLHPLSKYCHEPIVELAGECIDQQDIEINDYQF
NPEILTNCTHDQMVKESLVSHLLKSNCLITNQPDWGTLQIRYEGKQIDREKLLRYIISFRQHNEFHEQCVERIFCDLMQF
AKPDKLTVYARYTRRGGLDINPFRSNFEAVPDNQRLARQ

Sequences:

>Translated_279_residues
MQYQHDSLDKLKLGQQTQYASNYDHTLLQPVPRHLNRDTLGITHTQPFHFGADIWTAYEISWLNLNGLPQVAIADVAIDF
QSENLIESKSFKLYLNSFNQSKFATFEEVQQHLTQDLSNCAKGKVSVKLHPLSKYCHEPIVELAGECIDQQDIEINDYQF
NPEILTNCTHDQMVKESLVSHLLKSNCLITNQPDWGTLQIRYEGKQIDREKLLRYIISFRQHNEFHEQCVERIFCDLMQF
AKPDKLTVYARYTRRGGLDINPFRSNFEAVPDNQRLARQ
>Mature_279_residues
MQYQHDSLDKLKLGQQTQYASNYDHTLLQPVPRHLNRDTLGITHTQPFHFGADIWTAYEISWLNLNGLPQVAIADVAIDF
QSENLIESKSFKLYLNSFNQSKFATFEEVQQHLTQDLSNCAKGKVSVKLHPLSKYCHEPIVELAGECIDQQDIEINDYQF
NPEILTNCTHDQMVKESLVSHLLKSNCLITNQPDWGTLQIRYEGKQIDREKLLRYIISFRQHNEFHEQCVERIFCDLMQF
AKPDKLTVYARYTRRGGLDINPFRSNFEAVPDNQRLARQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1789158, Length=277, Percent_Identity=62.4548736462094, Blast_Score=357, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_PASMU (Q9CNF6)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245413.1
- ProteinModelPortal:   Q9CNF6
- SMR:   Q9CNF6
- GeneID:   1243823
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0476
- NMPDR:   fig|272843.1.peg.476
- HOGENOM:   HBG289139
- OMA:   PFRSNFE
- ProtClustDB:   PRK11792
- BioCyc:   PMUL272843:PM0476-MONOMER
- BRENDA:   1.7.1.13
- GO:   GO:0005737
- HAMAP:   MF_00817
- InterPro:   IPR016428
- InterPro:   IPR020602
- PIRSF:   PIRSF004750
- TIGRFAMs:   TIGR03138

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 32549; Mature: 32549

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYQHDSLDKLKLGQQTQYASNYDHTLLQPVPRHLNRDTLGITHTQPFHFGADIWTAYEI
CCCCCCCHHHHHCCCCHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEE
SWLNLNGLPQVAIADVAIDFQSENLIESKSFKLYLNSFNQSKFATFEEVQQHLTQDLSNC
EEEECCCCCCEEEEEEEEECCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
AKGKVSVKLHPLSKYCHEPIVELAGECIDQQDIEINDYQFNPEILTNCTHDQMVKESLVS
HCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCEECCEEECHHHHHCCCHHHHHHHHHHH
HLLKSNCLITNQPDWGTLQIRYEGKQIDREKLLRYIISFRQHNEFHEQCVERIFCDLMQF
HHHHCCCEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
AKPDKLTVYARYTRRGGLDINPFRSNFEAVPDNQRLARQ
CCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHCCC
>Mature Secondary Structure
MQYQHDSLDKLKLGQQTQYASNYDHTLLQPVPRHLNRDTLGITHTQPFHFGADIWTAYEI
CCCCCCCHHHHHCCCCHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEE
SWLNLNGLPQVAIADVAIDFQSENLIESKSFKLYLNSFNQSKFATFEEVQQHLTQDLSNC
EEEECCCCCCEEEEEEEEECCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
AKGKVSVKLHPLSKYCHEPIVELAGECIDQQDIEINDYQFNPEILTNCTHDQMVKESLVS
HCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCEECCEEECHHHHHCCCHHHHHHHHHHH
HLLKSNCLITNQPDWGTLQIRYEGKQIDREKLLRYIISFRQHNEFHEQCVERIFCDLMQF
HHHHCCCEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
AKPDKLTVYARYTRRGGLDINPFRSNFEAVPDNQRLARQ
CCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100