Click here to switch to the map view.

The map label for this gene is rfe

Identifier: 15602328

GI number: 15602328

Start: 539456

End: 540529

Strand: Direct

Name: rfe

Synonym: PM0463

Alternate gene names: 15602328

Gene position: 539456-540529 (Clockwise)

Preceding gene: 15602325

Following gene: 15602329

Centisome position: 23.9

GC content: 37.62

Gene sequence:

>1074_bases
ATGCTATTGAGTTTAATTGTGACATTTTTGGGGGCATTTCTGACATTATTATTTATGCGCCCGATTGCCCAAAAAATAGG
TTTAGTGGATAAACCGAATTTTCGTAAGCGCCATCAGGGTGCGATTCCTCTAATTGGTGGTGTGTCGTTGTTTGTTGGGA
ATCTCTGCTTCTATTTATTAGAGTGGGAACAAATGCGCTTACCAACACTTTATTTATTTAGTATTTTTGTGTTGCTTGTG
ATCGGTATGATTGATGACCGTTTTGACATCAGCCCTTTTTTGCGTGCCGGTATTCAAGCAATATTAGCTATATTAATGAT
TGATCTCGGTAATCTTTATTTAGATCATTTGGGGCAAATTCTCGGTCCATTTCAGCTGACACTTGGATCCATTGGCTTAA
TTATTACAGTGCTAGCCACTATAGCTGCAATTAATGCCTTTAATATGATTGATGGGATTGATGGTTTACTAGGCGGATTA
TCGAGTGTGGCTTTTGTGTCTATTGGCATTTTATTAATTAAGGATAATCAGCTTGACTTGGCTTATTGGAGCTTTGCCTT
AATTATTGCCATTCTGCCTTATTTTATGATGAATTTAGGCATTCCTTTTGGGCAGAAATTTAAAGTATTTATGGGCGATG
CGGGCAGTACTTTAATTGGCTTTACGATTATTTGGATTTTACTGCTCAGTACGCAAGGTAAAGGGCATCCAATGAATCCT
GTGACAGCATTATGGATAATCGCGATCCCACTTATTGATATGATTGCGATTATGTATCGCCGTTTACGTAAAGGCAAAAG
CCCATTCCGTCCAGACCGTTTACATGTACATCATTTAATGATGCGTGCCGGTTTAACATCTCGTCAAGCTTTCTTATTAA
TTACTTTCGCAGCGGCAATTTGTGCCACGATTGGTATTTTAGGAGAGATTTTTTATATTAATGAATGGCTGATGTTTGCC
GGATTTATTCTATTATTTTTTATGTATTCTTATTCAATTACACGTGCGTGGCGTATTACACGTTGGATTAGACGAATGAG
AAGACGCGCAAAACGTATTCATAATAAGGGTTAA

Upstream 100 bases:

>100_bases
ACCATTCTACTGTAAAAAAGCGGAAATAGATAAAAATATTTATGGAACAAAAAATGCTTTGTGGTATTCTTCGAGCATTC
AAATTCAAAGGGAAATTCCA

Downstream 100 bases:

>100_bases
ACAGCATGACGCAAGTAGAAAATACACAAAAAACACACCGCACTTTTCTAAGCTCAATCAGTATAATATTGGGGACTACG
TTGCTTGGCGCGATAGTGGC

Product: hypothetical protein

Products: UMP; Undecaprenyl N-Acetyl-Alpha-D-Glucosaminyl Pyrophosphate. [C]

Alternate protein names: UDP-GlcNAc:undecaprenyl-phosphate GlcNAc-1-phosphate transferase; Undecaprenyl-Phosphate GlcNAc-1-phosphate transferase

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MLLSLIVTFLGAFLTLLFMRPIAQKIGLVDKPNFRKRHQGAIPLIGGVSLFVGNLCFYLLEWEQMRLPTLYLFSIFVLLV
IGMIDDRFDISPFLRAGIQAILAILMIDLGNLYLDHLGQILGPFQLTLGSIGLIITVLATIAAINAFNMIDGIDGLLGGL
SSVAFVSIGILLIKDNQLDLAYWSFALIIAILPYFMMNLGIPFGQKFKVFMGDAGSTLIGFTIIWILLLSTQGKGHPMNP
VTALWIIAIPLIDMIAIMYRRLRKGKSPFRPDRLHVHHLMMRAGLTSRQAFLLITFAAAICATIGILGEIFYINEWLMFA
GFILLFFMYSYSITRAWRITRWIRRMRRRAKRIHNKG

Sequences:

>Translated_357_residues
MLLSLIVTFLGAFLTLLFMRPIAQKIGLVDKPNFRKRHQGAIPLIGGVSLFVGNLCFYLLEWEQMRLPTLYLFSIFVLLV
IGMIDDRFDISPFLRAGIQAILAILMIDLGNLYLDHLGQILGPFQLTLGSIGLIITVLATIAAINAFNMIDGIDGLLGGL
SSVAFVSIGILLIKDNQLDLAYWSFALIIAILPYFMMNLGIPFGQKFKVFMGDAGSTLIGFTIIWILLLSTQGKGHPMNP
VTALWIIAIPLIDMIAIMYRRLRKGKSPFRPDRLHVHHLMMRAGLTSRQAFLLITFAAAICATIGILGEIFYINEWLMFA
GFILLFFMYSYSITRAWRITRWIRRMRRRAKRIHNKG
>Mature_357_residues
MLLSLIVTFLGAFLTLLFMRPIAQKIGLVDKPNFRKRHQGAIPLIGGVSLFVGNLCFYLLEWEQMRLPTLYLFSIFVLLV
IGMIDDRFDISPFLRAGIQAILAILMIDLGNLYLDHLGQILGPFQLTLGSIGLIITVLATIAAINAFNMIDGIDGLLGGL
SSVAFVSIGILLIKDNQLDLAYWSFALIIAILPYFMMNLGIPFGQKFKVFMGDAGSTLIGFTIIWILLLSTQGKGHPMNP
VTALWIIAIPLIDMIAIMYRRLRKGKSPFRPDRLHVHHLMMRAGLTSRQAFLLITFAAAICATIGILGEIFYINEWLMFA
GFILLFFMYSYSITRAWRITRWIRRMRRRAKRIHNKG

Specific function: Catalyzes the transfer of the GlcNAc-1-phosphate moiety from UDP-GlcNAc onto the carrier lipid undecaprenyl phosphate (C55-P), yielding GlcNAc-pyrophosphoryl-undecaprenyl (GlcNAc-PP- C55)

COG id: COG0472

COG function: function code M; UDP-N-acetylmuramyl pentapeptide phosphotransferase/UDP-N-acetylglucosamine-1-phosphate transferase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 4 family. WecA subfamily

Homologues:

Organism=Escherichia coli, GI1790218, Length=327, Percent_Identity=60.8562691131498, Blast_Score=374, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): WECA_PASMU (Q9CNG8)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245400.1
- GeneID:   1243810
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0463
- NMPDR:   fig|272843.1.peg.463
- HOGENOM:   HBG536396
- OMA:   IPLIDMI
- ProtClustDB:   CLSK870116
- BioCyc:   PMUL272843:PM0463-MONOMER
- InterPro:   IPR012750
- InterPro:   IPR000715
- InterPro:   IPR018480
- PANTHER:   PTHR22926
- TIGRFAMs:   TIGR02380

Pfam domain/function: PF00953 Glycos_transf_4

EC number: 2.7.8.- [C]

Molecular weight: Translated: 40287; Mature: 40287

Theoretical pI: Translated: 10.76; Mature: 10.76

Prosite motif: PS01348 MRAY_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xd03dce4)-; HASH(0xca8be64)-; HASH(0xd149f90)-; HASH(0xcb96044)-; HASH(0xcc72754)-; HASH(0xca02354)-; HASH(0xc02c768)-; HASH(0xd144034)-; HASH(0xcbf2fd0)-; HASH(0xc6c2610)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLSLIVTFLGAFLTLLFMRPIAQKIGLVDKPNFRKRHQGAIPLIGGVSLFVGNLCFYLL
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHH
EWEQMRLPTLYLFSIFVLLVIGMIDDRFDISPFLRAGIQAILAILMIDLGNLYLDHLGQI
HHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGPFQLTLGSIGLIITVLATIAAINAFNMIDGIDGLLGGLSSVAFVSIGILLIKDNQLDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCHH
AYWSFALIIAILPYFMMNLGIPFGQKFKVFMGDAGSTLIGFTIIWILLLSTQGKGHPMNP
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCH
VTALWIIAIPLIDMIAIMYRRLRKGKSPFRPDRLHVHHLMMRAGLTSRQAFLLITFAAAI
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
CATIGILGEIFYINEWLMFAGFILLFFMYSYSITRAWRITRWIRRMRRRAKRIHNKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
>Mature Secondary Structure
MLLSLIVTFLGAFLTLLFMRPIAQKIGLVDKPNFRKRHQGAIPLIGGVSLFVGNLCFYLL
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHH
EWEQMRLPTLYLFSIFVLLVIGMIDDRFDISPFLRAGIQAILAILMIDLGNLYLDHLGQI
HHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGPFQLTLGSIGLIITVLATIAAINAFNMIDGIDGLLGGLSSVAFVSIGILLIKDNQLDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCHH
AYWSFALIIAILPYFMMNLGIPFGQKFKVFMGDAGSTLIGFTIIWILLLSTQGKGHPMNP
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCH
VTALWIIAIPLIDMIAIMYRRLRKGKSPFRPDRLHVHHLMMRAGLTSRQAFLLITFAAAI
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
CATIGILGEIFYINEWLMFAGFILLFFMYSYSITRAWRITRWIRRMRRRAKRIHNKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-N-Acetyl-D-Glucosamine; Undecaprenyl Phosphate [C]

Specific reaction: UDP-N-Acetyl-D-Glucosamine + Undecaprenyl Phosphate = UMP + Undecaprenyl N-Acetyl-Alpha-D-Glucosaminyl Pyrophosphate. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11248100