Click here to switch to the map view.

The map label for this gene is hemL

Identifier: 15602327

GI number: 15602327

Start: 538040

End: 539323

Strand: Reverse

Name: hemL

Synonym: PM0462

Alternate gene names: 15602327

Gene position: 539323-538040 (Counterclockwise)

Preceding gene: 15602330

Following gene: 15602326

Centisome position: 23.89

GC content: 44.16

Gene sequence:

>1284_bases
ATGACAACCTCTTTATCTCTTTTTCAACGTGCACAACATCGAATTCCCGGCGGTGTAAACTCACCAGTACGTGCCTTTAA
GGGAGTCGGTGGCACGCCGATTTTTATCGAAAAAGCCCAAGGTGCTTATCTTTATGATACTGAAGGCAATCAATATATTG
ATTATGTGGGATCTTGGGGACCGATGATTTTAGGGCATAACCACCCTACCATCTTAGATGCAGTCATCAGCACCGCTCAA
AATGGGTTAAGTTTTGGCGCACCAACTGCGTTAGAAATTGAATTAGCAGAATTAGTTTGTTCATTAGTCCCCTCAATAGA
CATGGTACGTATGGTCAGTTCTGGGACAGAAGCGACTATGTCGGCTATTCGCTTAGCTCGTGGATATACTAAACGAGATA
AAATCATTAAATTTGAAGGCTGTTACCATGGTCATGCTGATTCTTTGCTAGTAAAAGCTGGCTCGGGCGCGCTAACTTTA
GGGCAACCAAGCTCACCCGGTGTACCTGCAGATTTTGCCAAACATACTTTAACCTGTACATATAATGACCTAGATTCAGT
CAAGCACGCTTTTGAACAATATCCTAATGAAATTGCTTGTCTGATTGTCGAGCCTGTGGCCGGTAACATGAACTGTATTC
CACCTCAAGAGGGCTTTTTACAAGGCTTACGTGCACTCTGTGATCAATATGGTGCCGTCTTTATTATTGATGAAGTGATG
ACCGGTTTCCGTGTCGCATTAGGTGGTGCACAAACTTATTATGGCGTCACGCCGGACTTAACTTGTTTAGGCAAAGTGAT
TGGCGGCGGGATGCCTGTTGGTGCATTTGGCGGTAAAAAAGCCATTATGCAACACATTGCTCCGCTAGGTCCGGTTTATC
AAGCGGGAACATTGTCAGGCAACCCGATTGCCATGGCAGCCGGTCTTGCTTGCCTTACGGAGTTGAAAAAAGCGGGAAAT
GCACAACGTTTAGCCCAACAAACCGAGAAATTAGCACTAGGCTTAAAAGCCTTAGCAGACAAACATCATGTCCCGTTTGT
AGTGAATTATGTTGGTGGGATGTTTGGCATTTTCTTTACTGAACAAAAAACTGTTTCTTCTTATCAAGCGGTGATGGCTT
GTGATACAGAAAAATTCAACCGCTTTTTCCATGCGATGTTAGCACAAGGTATTTATCTTGCCCCTTCGGCGTTTGAAGCA
GGCTTTATGTCACTCGCCCATTCTGATCAAGATATTGAGCGCACTTTGCAAGCTGCCGATCAAGTTTTCTCTCAGTTAGC
TTAA

Upstream 100 bases:

>100_bases
AATACCACAAAGCATTTTTTGTTCCATAAATATTTTTATCTATTTCCGCTTTTTTACAGTAGAATGGTGCTCATTTCTAT
CACAAAATTAGGATACGATT

Downstream 100 bases:

>100_bases
AGGCAGTGTATGGCGATAATTAAGGATAATTACAGCTTAGCATTGCTTAAGGTACACGGCACCGCAGTGTTATTTGGTGC
CTCTGGTGTTTTTGGCGCAT

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MTTSLSLFQRAQHRIPGGVNSPVRAFKGVGGTPIFIEKAQGAYLYDTEGNQYIDYVGSWGPMILGHNHPTILDAVISTAQ
NGLSFGAPTALEIELAELVCSLVPSIDMVRMVSSGTEATMSAIRLARGYTKRDKIIKFEGCYHGHADSLLVKAGSGALTL
GQPSSPGVPADFAKHTLTCTYNDLDSVKHAFEQYPNEIACLIVEPVAGNMNCIPPQEGFLQGLRALCDQYGAVFIIDEVM
TGFRVALGGAQTYYGVTPDLTCLGKVIGGGMPVGAFGGKKAIMQHIAPLGPVYQAGTLSGNPIAMAAGLACLTELKKAGN
AQRLAQQTEKLALGLKALADKHHVPFVVNYVGGMFGIFFTEQKTVSSYQAVMACDTEKFNRFFHAMLAQGIYLAPSAFEA
GFMSLAHSDQDIERTLQAADQVFSQLA

Sequences:

>Translated_427_residues
MTTSLSLFQRAQHRIPGGVNSPVRAFKGVGGTPIFIEKAQGAYLYDTEGNQYIDYVGSWGPMILGHNHPTILDAVISTAQ
NGLSFGAPTALEIELAELVCSLVPSIDMVRMVSSGTEATMSAIRLARGYTKRDKIIKFEGCYHGHADSLLVKAGSGALTL
GQPSSPGVPADFAKHTLTCTYNDLDSVKHAFEQYPNEIACLIVEPVAGNMNCIPPQEGFLQGLRALCDQYGAVFIIDEVM
TGFRVALGGAQTYYGVTPDLTCLGKVIGGGMPVGAFGGKKAIMQHIAPLGPVYQAGTLSGNPIAMAAGLACLTELKKAGN
AQRLAQQTEKLALGLKALADKHHVPFVVNYVGGMFGIFFTEQKTVSSYQAVMACDTEKFNRFFHAMLAQGIYLAPSAFEA
GFMSLAHSDQDIERTLQAADQVFSQLA
>Mature_426_residues
TTSLSLFQRAQHRIPGGVNSPVRAFKGVGGTPIFIEKAQGAYLYDTEGNQYIDYVGSWGPMILGHNHPTILDAVISTAQN
GLSFGAPTALEIELAELVCSLVPSIDMVRMVSSGTEATMSAIRLARGYTKRDKIIKFEGCYHGHADSLLVKAGSGALTLG
QPSSPGVPADFAKHTLTCTYNDLDSVKHAFEQYPNEIACLIVEPVAGNMNCIPPQEGFLQGLRALCDQYGAVFIIDEVMT
GFRVALGGAQTYYGVTPDLTCLGKVIGGGMPVGAFGGKKAIMQHIAPLGPVYQAGTLSGNPIAMAAGLACLTELKKAGNA
QRLAQQTEKLALGLKALADKHHVPFVVNYVGGMFGIFFTEQKTVSSYQAVMACDTEKFNRFFHAMLAQGIYLAPSAFEAG
FMSLAHSDQDIERTLQAADQVFSQLA

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=311, Percent_Identity=30.8681672025724, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI37574042, Length=347, Percent_Identity=28.2420749279539, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI13994255, Length=368, Percent_Identity=26.6304347826087, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI226442705, Length=354, Percent_Identity=27.683615819209, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI284507298, Length=267, Percent_Identity=29.9625468164794, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI226442709, Length=316, Percent_Identity=27.5316455696203, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1786349, Length=426, Percent_Identity=72.3004694835681, Blast_Score=657, Evalue=0.0,
Organism=Escherichia coli, GI1789016, Length=380, Percent_Identity=31.8421052631579, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1787560, Length=376, Percent_Identity=30.5851063829787, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI1788044, Length=283, Percent_Identity=32.5088339222615, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI145693181, Length=314, Percent_Identity=30.2547770700637, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1789759, Length=278, Percent_Identity=32.0143884892086, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1786991, Length=389, Percent_Identity=26.7352185089974, Blast_Score=93, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71992977, Length=427, Percent_Identity=27.6346604215457, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI25144271, Length=318, Percent_Identity=28.3018867924528, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI25144274, Length=163, Percent_Identity=32.5153374233129, Blast_Score=78, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6323470, Length=339, Percent_Identity=29.2035398230088, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6324432, Length=353, Percent_Identity=24.6458923512748, Blast_Score=83, Evalue=8e-17,
Organism=Drosophila melanogaster, GI21357415, Length=308, Percent_Identity=29.5454545454545, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21356575, Length=351, Percent_Identity=26.7806267806268, Blast_Score=100, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_PASMU (Q9CNG9)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245399.1
- ProteinModelPortal:   Q9CNG9
- SMR:   Q9CNG9
- GeneID:   1243809
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0462
- NMPDR:   fig|272843.1.peg.462
- HOGENOM:   HBG725944
- OMA:   VGCFGGK
- ProtClustDB:   PRK00062
- BioCyc:   PMUL272843:PM0462-MONOMER
- BRENDA:   5.4.3.8
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 45514; Mature: 45383

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSLSLFQRAQHRIPGGVNSPVRAFKGVGGTPIFIEKAQGAYLYDTEGNQYIDYVGSWG
CCCCHHHHHHHHHHCCCCCCCHHHHHCCCCCCEEEEEECCCCEEEECCCCCEEHCCCCCC
PMILGHNHPTILDAVISTAQNGLSFGAPTALEIELAELVCSLVPSIDMVRMVSSGTEATM
CEEECCCCCHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHCCHHHHHHHHCCHHHHH
SAIRLARGYTKRDKIIKFEGCYHGHADSLLVKAGSGALTLGQPSSPGVPADFAKHTLTCT
HHHHHHHCCCCCCCEEEEECCCCCCCCCEEEECCCCEEECCCCCCCCCCHHHHCCEEEEE
YNDLDSVKHAFEQYPNEIACLIVEPVAGNMNCIPPQEGFLQGLRALCDQYGAVFIIDEVM
CCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEEHHHHH
TGFRVALGGAQTYYGVTPDLTCLGKVIGGGMPVGAFGGKKAIMQHIAPLGPVYQAGTLSG
HHHHHHHCCCCEECCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCC
NPIAMAAGLACLTELKKAGNAQRLAQQTEKLALGLKALADKHHVPFVVNYVGGMFGIFFT
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCC
EQKTVSSYQAVMACDTEKFNRFFHAMLAQGIYLAPSAFEAGFMSLAHSDQDIERTLQAAD
CCHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHCCCHHHHHHHHHHH
QVFSQLA
HHHHHCC
>Mature Secondary Structure 
TTSLSLFQRAQHRIPGGVNSPVRAFKGVGGTPIFIEKAQGAYLYDTEGNQYIDYVGSWG
CCCHHHHHHHHHHCCCCCCCHHHHHCCCCCCEEEEEECCCCEEEECCCCCEEHCCCCCC
PMILGHNHPTILDAVISTAQNGLSFGAPTALEIELAELVCSLVPSIDMVRMVSSGTEATM
CEEECCCCCHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHCCHHHHHHHHCCHHHHH
SAIRLARGYTKRDKIIKFEGCYHGHADSLLVKAGSGALTLGQPSSPGVPADFAKHTLTCT
HHHHHHHCCCCCCCEEEEECCCCCCCCCEEEECCCCEEECCCCCCCCCCHHHHCCEEEEE
YNDLDSVKHAFEQYPNEIACLIVEPVAGNMNCIPPQEGFLQGLRALCDQYGAVFIIDEVM
CCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEEHHHHH
TGFRVALGGAQTYYGVTPDLTCLGKVIGGGMPVGAFGGKKAIMQHIAPLGPVYQAGTLSG
HHHHHHHCCCCEECCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCC
NPIAMAAGLACLTELKKAGNAQRLAQQTEKLALGLKALADKHHVPFVVNYVGGMFGIFFT
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCC
EQKTVSSYQAVMACDTEKFNRFFHAMLAQGIYLAPSAFEAGFMSLAHSDQDIERTLQAAD
CCHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHCCCHHHHHHHHHHH
QVFSQLA
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100