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The map label for this gene is ponB [H]

Identifier: 15602321

GI number: 15602321

Start: 530422

End: 532749

Strand: Reverse

Name: ponB [H]

Synonym: PM0456

Alternate gene names: 15602321

Gene position: 532749-530422 (Counterclockwise)

Preceding gene: 15602322

Following gene: 15602318

Centisome position: 23.6

GC content: 42.53

Gene sequence:

>2328_bases
ATGCCAAAATCCAACAAAACCAATGTAAGCCGAAAAAAATCTTCTTGGCCCCTCTTATGTGCCAAAGTGAGTTTTACATT
AATCTGTATGTTGGCGTTTTATGGATTGTATTTAGATTGGCAAATACGCTCTAAAATGGATGGGCAGATCTGGCGACTCC
CCGCAGAAGTCTATAGCCGTATTGAACGTATCAGTCTTGAAGAACAACTGAGCTTTGAACAAGTCAAACAACGTTTATTA
GACAATGATTATCGTCAAACCACCCTAGTAGCAGCCCCTGGCGATTTTAAAGTGGAAGGTGACACCCTCGTTTTGCTACG
ACGCGCGTTTCCCTTTCCTGTACAACCAGAAGCACAGCGTGTCTTTCGTCTACGTTTTAAACACAATCAATTGGCAGTAA
TTGAAGATTTAATCAACCAACGTACCATTCCTGAGTTTCGTTTAGCGCCAAAATTAATCGCTATGCTACAGTCAGAGAAA
GAAGAGCGTTTAGCGATCCCACTCCATAACTATCCACGTTTATTAATCGATGCATTATTACTTACCGAAGATCGTCGCTT
TTACCAACATGAAGGAATCAGTTTACTCGGCATTGCACGTGCGATGATCACCAATATTCAAGCAGGTCATACTGTACAAG
GTGGCAGTACCTTAACGCAACAATTAGTCAAAAACCTGTTTTTAAGTAACAAACGAACCCTAAGCCGTAAAGTCAATGAA
GCCTTGATGGCCGTGATTTTAGATTGGCGTTACGATAAAAATCGTATTTTAGAAACCTATTTAAATGAAATTTATTTAGG
ACAAAATGGCGATACTCAAATTCATGGCTTTGAACTGGCTAGCCACTTTTATTTTGGGCGCTCAACCAACGAAATTAGTT
TGGATCAAATCGCCTTATTAGTTGGCATGGTCAAAGGTCCTTCTCTTTATAATCCTTGGCGGAATCCGAACACCGCATTA
GAGCGCCGTAATGTGGTACTGCGTTTACTCCTCGATCATCAAGTAATTGGGCATGAACTGTATCAAATGTTAGTCCAGCG
TCCATTAGGCGTACAAGAACGTGGTCAAATTACGCGCAAGTACCCTGCCTTCATTCAGACCTTACAAGCTGAATTACGTG
AGCAATTAGGCGAAAACAAAGCCACGAATTTATTAGGAGCACGGATTTTTTCAACCTTAGATCCTAAACAACAAACCCAT
GCAGAAAATGCAGTAGTCAGTGCGACATCTACCTTACAAGCGAAACACAAAAACCCCCATTTACAATCTGCGATGGTAAT
TGCTGATTATCGTTTAGGCGAAGTGCGGGCCATTGTTGGTGGTTTACAAACACAATATGCGGGCTTTAATCGTGCCTTAA
GCGCCAAACGCCAAATTGGCTCTTTAGTAAAACCCGCCATTTATTTGACCGCACTTTCAGTACCTGACAAGTTCCGTCTT
AATACAGCGATTCAAAATCAACCGATTACCATTCAAATTAAAGGCAGCCCACCGTGGCAACCGCGTAACTATGATCGCAA
ATACAGCGGTTCCGTGATGTTAATGGATGCCTTAGCTCGTTCCTTAAACATCCCAACAGTAAATATTGGGATGCAAGTGG
GATTAGAGAAAGTGATTGAAACACAAAAGAAAATGGGTTGGGATAAAGTACAAATCCCCAAAGTACCCGCCACTTTATTG
GGCGCTTATGCGATTTCCCCTTACGAAGTCACGCATTTATATCAAGTGATCGCCAATCAAGGACAAAAAATCCCGTTGGC
GACCATTGACAGCATCACAGACCAACAAGGAAATCTCTTATATCAACGCCGCTTAGAACCTGAGCAAGTGGTACCGGCTG
AAGCCAGTTATCAAACACTGTATGCTATGCAACAAACCGTAACGCGAGGCACTGCGCGCAGTTTACAAAACCAATTTAGT
CACTTACATCTTGCCGGAAAAACAGGCACTACCAATGATGCACGTGATACCTGGTTTGTGGGCATTGATGGTGAAAACAT
CGTGACGGTTTGGTTAGGGCGTGATGATAATGGCGAAACCAAACTAACTGGCGCTTCTGGTGCCTTGCAAATTTATCAAA
ATTATTTACAGCGTCATCCGGCAACTGTACTTCACTTACCTTCTCTTAACAGTATTCAATGGGTAGGTATTAATGATTAT
GGTAGTTGGGATTGTTCAAGCGCACGTCAAATTCCCGTTTGGATAAATCATGCTCAGTCTTTTTGCCAAGCACCTGAAAT
CAGCAAACAAGAAAGCGTATGGGATGTGTTATCGCCGGGTGCTCCATCAAACCAACCGGCACCAGTAGAAGATGCCATTG
CTAACTGA

Upstream 100 bases:

>100_bases
AGTATTGCCTTTTGTGGCACAGATGACTTTATCCATTTCTTACAATCTGCACTAGCTTTCATCCAATCCTAATGAAATTA
CGCGAGTTTGAGTAGGTCAT

Downstream 100 bases:

>100_bases
GCAAACAGCATAAACAAAAAGTGCGGTCAATTTTTTTGCTTTTTTGACCGCACTTTTCTAAGTGAATATGAAGATTAGAG
AGTTTTTAATTTCTCTAACG

Product: PonB

Products: NA

Alternate protein names: PBP-1b; PBP1b; Murein polymerase; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Peptidoglycan glycosyltransferase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 775; Mature: 774

Protein sequence:

>775_residues
MPKSNKTNVSRKKSSWPLLCAKVSFTLICMLAFYGLYLDWQIRSKMDGQIWRLPAEVYSRIERISLEEQLSFEQVKQRLL
DNDYRQTTLVAAPGDFKVEGDTLVLLRRAFPFPVQPEAQRVFRLRFKHNQLAVIEDLINQRTIPEFRLAPKLIAMLQSEK
EERLAIPLHNYPRLLIDALLLTEDRRFYQHEGISLLGIARAMITNIQAGHTVQGGSTLTQQLVKNLFLSNKRTLSRKVNE
ALMAVILDWRYDKNRILETYLNEIYLGQNGDTQIHGFELASHFYFGRSTNEISLDQIALLVGMVKGPSLYNPWRNPNTAL
ERRNVVLRLLLDHQVIGHELYQMLVQRPLGVQERGQITRKYPAFIQTLQAELREQLGENKATNLLGARIFSTLDPKQQTH
AENAVVSATSTLQAKHKNPHLQSAMVIADYRLGEVRAIVGGLQTQYAGFNRALSAKRQIGSLVKPAIYLTALSVPDKFRL
NTAIQNQPITIQIKGSPPWQPRNYDRKYSGSVMLMDALARSLNIPTVNIGMQVGLEKVIETQKKMGWDKVQIPKVPATLL
GAYAISPYEVTHLYQVIANQGQKIPLATIDSITDQQGNLLYQRRLEPEQVVPAEASYQTLYAMQQTVTRGTARSLQNQFS
HLHLAGKTGTTNDARDTWFVGIDGENIVTVWLGRDDNGETKLTGASGALQIYQNYLQRHPATVLHLPSLNSIQWVGINDY
GSWDCSSARQIPVWINHAQSFCQAPEISKQESVWDVLSPGAPSNQPAPVEDAIAN

Sequences:

>Translated_775_residues
MPKSNKTNVSRKKSSWPLLCAKVSFTLICMLAFYGLYLDWQIRSKMDGQIWRLPAEVYSRIERISLEEQLSFEQVKQRLL
DNDYRQTTLVAAPGDFKVEGDTLVLLRRAFPFPVQPEAQRVFRLRFKHNQLAVIEDLINQRTIPEFRLAPKLIAMLQSEK
EERLAIPLHNYPRLLIDALLLTEDRRFYQHEGISLLGIARAMITNIQAGHTVQGGSTLTQQLVKNLFLSNKRTLSRKVNE
ALMAVILDWRYDKNRILETYLNEIYLGQNGDTQIHGFELASHFYFGRSTNEISLDQIALLVGMVKGPSLYNPWRNPNTAL
ERRNVVLRLLLDHQVIGHELYQMLVQRPLGVQERGQITRKYPAFIQTLQAELREQLGENKATNLLGARIFSTLDPKQQTH
AENAVVSATSTLQAKHKNPHLQSAMVIADYRLGEVRAIVGGLQTQYAGFNRALSAKRQIGSLVKPAIYLTALSVPDKFRL
NTAIQNQPITIQIKGSPPWQPRNYDRKYSGSVMLMDALARSLNIPTVNIGMQVGLEKVIETQKKMGWDKVQIPKVPATLL
GAYAISPYEVTHLYQVIANQGQKIPLATIDSITDQQGNLLYQRRLEPEQVVPAEASYQTLYAMQQTVTRGTARSLQNQFS
HLHLAGKTGTTNDARDTWFVGIDGENIVTVWLGRDDNGETKLTGASGALQIYQNYLQRHPATVLHLPSLNSIQWVGINDY
GSWDCSSARQIPVWINHAQSFCQAPEISKQESVWDVLSPGAPSNQPAPVEDAIAN
>Mature_774_residues
PKSNKTNVSRKKSSWPLLCAKVSFTLICMLAFYGLYLDWQIRSKMDGQIWRLPAEVYSRIERISLEEQLSFEQVKQRLLD
NDYRQTTLVAAPGDFKVEGDTLVLLRRAFPFPVQPEAQRVFRLRFKHNQLAVIEDLINQRTIPEFRLAPKLIAMLQSEKE
ERLAIPLHNYPRLLIDALLLTEDRRFYQHEGISLLGIARAMITNIQAGHTVQGGSTLTQQLVKNLFLSNKRTLSRKVNEA
LMAVILDWRYDKNRILETYLNEIYLGQNGDTQIHGFELASHFYFGRSTNEISLDQIALLVGMVKGPSLYNPWRNPNTALE
RRNVVLRLLLDHQVIGHELYQMLVQRPLGVQERGQITRKYPAFIQTLQAELREQLGENKATNLLGARIFSTLDPKQQTHA
ENAVVSATSTLQAKHKNPHLQSAMVIADYRLGEVRAIVGGLQTQYAGFNRALSAKRQIGSLVKPAIYLTALSVPDKFRLN
TAIQNQPITIQIKGSPPWQPRNYDRKYSGSVMLMDALARSLNIPTVNIGMQVGLEKVIETQKKMGWDKVQIPKVPATLLG
AYAISPYEVTHLYQVIANQGQKIPLATIDSITDQQGNLLYQRRLEPEQVVPAEASYQTLYAMQQTVTRGTARSLQNQFSH
LHLAGKTGTTNDARDTWFVGIDGENIVTVWLGRDDNGETKLTGASGALQIYQNYLQRHPATVLHLPSLNSIQWVGINDYG
SWDCSSARQIPVWINHAQSFCQAPEISKQESVWDVLSPGAPSNQPAPVEDAIAN

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=741, Percent_Identity=50.0674763832659, Blast_Score=710, Evalue=0.0,
Organism=Escherichia coli, GI87082258, Length=276, Percent_Identity=33.3333333333333, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI1788867, Length=588, Percent_Identity=26.0204081632653, Blast_Score=146, Evalue=6e-36,
Organism=Escherichia coli, GI1789601, Length=130, Percent_Identity=32.3076923076923, Blast_Score=73, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011813
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: =2.4.1.129 [H]

Molecular weight: Translated: 87554; Mature: 87422

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKSNKTNVSRKKSSWPLLCAKVSFTLICMLAFYGLYLDWQIRSKMDGQIWRLPAEVYSR
CCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHEEEEEECCCCCCCEEECCHHHHHH
IERISLEEQLSFEQVKQRLLDNDYRQTTLVAAPGDFKVEGDTLVLLRRAFPFPVQPEAQR
HHHHCHHHHCCHHHHHHHHHCCCCCCEEEEECCCCEEECCCEEEEEECCCCCCCCHHHHH
VFRLRFKHNQLAVIEDLINQRTIPEFRLAPKLIAMLQSEKEERLAIPLHNYPRLLIDALL
HHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCHHHHHHHHH
LTEDRRFYQHEGISLLGIARAMITNIQAGHTVQGGSTLTQQLVKNLFLSNKRTLSRKVNE
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHCCCHHHHHHHHH
ALMAVILDWRYDKNRILETYLNEIYLGQNGDTQIHGFELASHFYFGRSTNEISLDQIALL
HHHHHHHHCCCCHHHHHHHHHHHEEECCCCCCEEEHEEHHHHEEECCCCCCCCHHHHHHH
VGMVKGPSLYNPWRNPNTALERRNVVLRLLLDHQVIGHELYQMLVQRPLGVQERGQITRK
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCHHHH
YPAFIQTLQAELREQLGENKATNLLGARIFSTLDPKQQTHAENAVVSATSTLQAKHKNPH
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHEEHHHHHHHHCCCCC
LQSAMVIADYRLGEVRAIVGGLQTQYAGFNRALSAKRQIGSLVKPAIYLTALSVPDKFRL
HHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEECCCCCCEE
NTAIQNQPITIQIKGSPPWQPRNYDRKYSGSVMLMDALARSLNIPTVNIGMQVGLEKVIE
CHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEECCHHHHHHHHHH
TQKKMGWDKVQIPKVPATLLGAYAISPYEVTHLYQVIANQGQKIPLATIDSITDQQGNLL
HHHHCCCCCEECCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEHHHCCCCCCCCEE
YQRRLEPEQVVPAEASYQTLYAMQQTVTRGTARSLQNQFSHLHLAGKTGTTNDARDTWFV
EECCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCEEEE
GIDGENIVTVWLGRDDNGETKLTGASGALQIYQNYLQRHPATVLHLPSLNSIQWVGINDY
EECCCCEEEEEEECCCCCCEEEECCCHHHHHHHHHHHHCCCEEEECCCCCCEEEEEECCC
GSWDCSSARQIPVWINHAQSFCQAPEISKQESVWDVLSPGAPSNQPAPVEDAIAN
CCCCCCCCCCCCEEEHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHCC
>Mature Secondary Structure 
PKSNKTNVSRKKSSWPLLCAKVSFTLICMLAFYGLYLDWQIRSKMDGQIWRLPAEVYSR
CCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHEEEEEECCCCCCCEEECCHHHHHH
IERISLEEQLSFEQVKQRLLDNDYRQTTLVAAPGDFKVEGDTLVLLRRAFPFPVQPEAQR
HHHHCHHHHCCHHHHHHHHHCCCCCCEEEEECCCCEEECCCEEEEEECCCCCCCCHHHHH
VFRLRFKHNQLAVIEDLINQRTIPEFRLAPKLIAMLQSEKEERLAIPLHNYPRLLIDALL
HHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCHHHHHHHHH
LTEDRRFYQHEGISLLGIARAMITNIQAGHTVQGGSTLTQQLVKNLFLSNKRTLSRKVNE
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHCCCHHHHHHHHH
ALMAVILDWRYDKNRILETYLNEIYLGQNGDTQIHGFELASHFYFGRSTNEISLDQIALL
HHHHHHHHCCCCHHHHHHHHHHHEEECCCCCCEEEHEEHHHHEEECCCCCCCCHHHHHHH
VGMVKGPSLYNPWRNPNTALERRNVVLRLLLDHQVIGHELYQMLVQRPLGVQERGQITRK
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCHHHH
YPAFIQTLQAELREQLGENKATNLLGARIFSTLDPKQQTHAENAVVSATSTLQAKHKNPH
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHEEHHHHHHHHCCCCC
LQSAMVIADYRLGEVRAIVGGLQTQYAGFNRALSAKRQIGSLVKPAIYLTALSVPDKFRL
HHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEECCCCCCEE
NTAIQNQPITIQIKGSPPWQPRNYDRKYSGSVMLMDALARSLNIPTVNIGMQVGLEKVIE
CHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEECCHHHHHHHHHH
TQKKMGWDKVQIPKVPATLLGAYAISPYEVTHLYQVIANQGQKIPLATIDSITDQQGNLL
HHHHCCCCCEECCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEHHHCCCCCCCCEE
YQRRLEPEQVVPAEASYQTLYAMQQTVTRGTARSLQNQFSHLHLAGKTGTTNDARDTWFV
EECCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCEEEE
GIDGENIVTVWLGRDDNGETKLTGASGALQIYQNYLQRHPATVLHLPSLNSIQWVGINDY
EECCCCEEEEEEECCCCCCEEEECCCHHHHHHHHHHHHCCCEEEECCCCCCEEEEEECCC
GSWDCSSARQIPVWINHAQSFCQAPEISKQESVWDVLSPGAPSNQPAPVEDAIAN
CCCCCCCCCCCCEEEHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]