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The map label for this gene is metR [H]

Identifier: 15602286

GI number: 15602286

Start: 495524

End: 496450

Strand: Direct

Name: metR [H]

Synonym: PM0421

Alternate gene names: 15602286

Gene position: 495524-496450 (Clockwise)

Preceding gene: 15602283

Following gene: 15602287

Centisome position: 21.95

GC content: 43.37

Gene sequence:

>927_bases
ATGAAACCTATTTTTTTAGAACTCCGTCATTTAAAAACGTTACTGGCGTTAAAAGAAACTGGCAGTGTGTCTTTAGCGGC
AAAACGTGTGTATTTAACTCAGTCTGCGCTGTCACACCAGTTGAAATTACTGGAAGAACAATATGGGCTTCCTTTATTTG
AACGCAAAACACAACCGTTACATTTTACTCCTGCGGGGGAAAGGTTGATTCAGCTTGCTAATGAAATTTTGCCGAAAGTA
GTAGAAGCGGAGCGCGATCTGGCTCGGGTGAAACAAGGGGAGGCAGGGGAGCTACGCATTGCGGTGGAATGTCATACTTG
CTTCGACTGGCTGATGCCAGCGATGGATTCCTTACGTCAAAATTGGCCTTTAGTGGAATTAGATATTGTCTCGGGCTTTC
ATACTGATCCAGTGGGCTTATTATTAACGCACCGTGCCGATTGGGCGGTGGTATCTGAGATTGAAGAAACCGCAGGTATT
ATCCATAAGCCCTTGTTTTCTTATGAAATGGTGGGATTGTGCGCCAAAGATCACCCTTTAGCAACGAAAGAGATTTGGGA
ACCTGAAGATTTTATTGATCAAACCTTGATTACTTACCCTGTCCCTGATGATATGTTGGATTTATTACGCAAAGTATTAC
ACCCGAATGGGATTAATCCTCTGCGCCGTACCAGTGAATTAACCATTGCGATTATTCAGCTGGTGGCAAGCAAACGTGGG
ATTGCGGCGTTGCCATTTTGGGCAGCCAAACCCTATTTAGATCGTGGCTATTTGGTGGCGCGTAAAATTACCAAACAAGG
CTTATACAGTAATTTATATGCGGCAATTCGTGAAATTGATGCCAATGTAGCGTTTGTCGATGATTTTTATGAAACCGTAA
AAGCACAGAGTTTTTCGACGTTACCCGCACTATCCGTATTGGAATAA

Upstream 100 bases:

>100_bases
GATAAAACTGAGCGCACTTTTATTTGCTTTTAGTGATCCAATAGCGATTTTGGTATATGATAACGTTCAATTCTTTCCCT
TATTTCGATGAATCTTTTTA

Downstream 100 bases:

>100_bases
TCAAGATGTTAGATGTTAATAAGCACCCTATTTTTAGGGCAGCTAAAGCCGCATTTCCTTATAGTTTACCCATTTTGTCT
GGTTTTCTGTTTTTAGGGAT

Product: MetR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MKPIFLELRHLKTLLALKETGSVSLAAKRVYLTQSALSHQLKLLEEQYGLPLFERKTQPLHFTPAGERLIQLANEILPKV
VEAERDLARVKQGEAGELRIAVECHTCFDWLMPAMDSLRQNWPLVELDIVSGFHTDPVGLLLTHRADWAVVSEIEETAGI
IHKPLFSYEMVGLCAKDHPLATKEIWEPEDFIDQTLITYPVPDDMLDLLRKVLHPNGINPLRRTSELTIAIIQLVASKRG
IAALPFWAAKPYLDRGYLVARKITKQGLYSNLYAAIREIDANVAFVDDFYETVKAQSFSTLPALSVLE

Sequences:

>Translated_308_residues
MKPIFLELRHLKTLLALKETGSVSLAAKRVYLTQSALSHQLKLLEEQYGLPLFERKTQPLHFTPAGERLIQLANEILPKV
VEAERDLARVKQGEAGELRIAVECHTCFDWLMPAMDSLRQNWPLVELDIVSGFHTDPVGLLLTHRADWAVVSEIEETAGI
IHKPLFSYEMVGLCAKDHPLATKEIWEPEDFIDQTLITYPVPDDMLDLLRKVLHPNGINPLRRTSELTIAIIQLVASKRG
IAALPFWAAKPYLDRGYLVARKITKQGLYSNLYAAIREIDANVAFVDDFYETVKAQSFSTLPALSVLE
>Mature_308_residues
MKPIFLELRHLKTLLALKETGSVSLAAKRVYLTQSALSHQLKLLEEQYGLPLFERKTQPLHFTPAGERLIQLANEILPKV
VEAERDLARVKQGEAGELRIAVECHTCFDWLMPAMDSLRQNWPLVELDIVSGFHTDPVGLLLTHRADWAVVSEIEETAGI
IHKPLFSYEMVGLCAKDHPLATKEIWEPEDFIDQTLITYPVPDDMLDLLRKVLHPNGINPLRRTSELTIAIIQLVASKRG
IAALPFWAAKPYLDRGYLVARKITKQGLYSNLYAAIREIDANVAFVDDFYETVKAQSFSTLPALSVLE

Specific function: Control of the last step in methionine biosynthesis; metR is a positive activator of the metA, metE and metH genes [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790262, Length=298, Percent_Identity=41.6107382550336, Blast_Score=241, Evalue=4e-65,
Organism=Escherichia coli, GI1787879, Length=255, Percent_Identity=30.5882352941176, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI145693105, Length=241, Percent_Identity=26.1410788381743, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1786401, Length=304, Percent_Identity=25.9868421052632, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI157672245, Length=269, Percent_Identity=26.3940520446097, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1790208, Length=156, Percent_Identity=30.1282051282051, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1789204, Length=264, Percent_Identity=26.5151515151515, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787530, Length=198, Percent_Identity=26.2626262626263, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790399, Length=260, Percent_Identity=27.3076923076923, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788297, Length=258, Percent_Identity=26.7441860465116, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1786713, Length=178, Percent_Identity=30.3370786516854, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1789173, Length=242, Percent_Identity=25.6198347107438, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34776; Mature: 34776

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPIFLELRHLKTLLALKETGSVSLAAKRVYLTQSALSHQLKLLEEQYGLPLFERKTQPL
CCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCE
HFTPAGERLIQLANEILPKVVEAERDLARVKQGEAGELRIAVECHTCFDWLMPAMDSLRQ
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHH
NWPLVELDIVSGFHTDPVGLLLTHRADWAVVSEIEETAGIIHKPLFSYEMVGLCAKDHPL
CCCEEEEEECCCCCCCCHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ATKEIWEPEDFIDQTLITYPVPDDMLDLLRKVLHPNGINPLRRTSELTIAIIQLVASKRG
CHHHCCCCHHHHCCCEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCC
IAALPFWAAKPYLDRGYLVARKITKQGLYSNLYAAIREIDANVAFVDDFYETVKAQSFST
CEECCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCC
LPALSVLE
CCHHHHCC
>Mature Secondary Structure
MKPIFLELRHLKTLLALKETGSVSLAAKRVYLTQSALSHQLKLLEEQYGLPLFERKTQPL
CCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCE
HFTPAGERLIQLANEILPKVVEAERDLARVKQGEAGELRIAVECHTCFDWLMPAMDSLRQ
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHH
NWPLVELDIVSGFHTDPVGLLLTHRADWAVVSEIEETAGIIHKPLFSYEMVGLCAKDHPL
CCCEEEEEECCCCCCCCHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ATKEIWEPEDFIDQTLITYPVPDDMLDLLRKVLHPNGINPLRRTSELTIAIIQLVASKRG
CHHHCCCCHHHHCCCEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCC
IAALPFWAAKPYLDRGYLVARKITKQGLYSNLYAAIREIDANVAFVDDFYETVKAQSFST
CEECCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCC
LPALSVLE
CCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]