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The map label for this gene is tbpA [H]

Identifier: 15602241

GI number: 15602241

Start: 444251

End: 445255

Strand: Direct

Name: tbpA [H]

Synonym: PM0376

Alternate gene names: 15602241

Gene position: 444251-445255 (Clockwise)

Preceding gene: 15602239

Following gene: 15602242

Centisome position: 19.68

GC content: 40.4

Gene sequence:

>1005_bases
ATGTCTAGATTAAAAACTTCCTTTTTTTTCACCGCACTTTCCACACTGTCACTATCCGTCTTTGCTCAAACGCAAGCGGT
CAATGTGTATACTTATGATTCCTTCACTTCTGAATGGGGGGCAGGTCCGAAAGTAAAAAAAGCATTTGAAACCCATTTCC
CACAATGCCAAGTGAATTTTACTGCGTTTGGTGATTCTGGCACTATGTTTAATCGCTTACGTTTAGAAGGGAAAAAAACA
AAAGCGGATGTGGTTGTAGGGTTAGATAACTACAACCTTGAGGAGGCAGAAAAAAGCGGCTTATTTGTTCAACATAAAGT
CGATTTAACACCGCTGTCACTACCGGTTGAGTGGAAAAATCAAACTTTTTTACCTTATGATTTTGGGCAGTTTGCCTTTA
TTTATGACAAAACCAAAGTGCAACAGCCACCAAAAAGCTTAAAAGAATTGGTAGAACGTGATGATTTAAAAGTCATTTAT
CAAGATCCACGTACTAGCAGTGTAGGACGTGGCTTACTCATTTGGATGAACCATGTTTATACCGAGAATGAAGTGGCACA
AGCTTGGCAAAAATTAGCCAAACATACGGTGACTGTGGGGAAAGGTTGGTCTGATACTTATGGCGCGTTTTTGAAAGGCG
AAGCTGATGTTGTCTTGAGCTATAACACCTCTCCGTTATATCACATGGTGTTTGAACAAAAAGATCAGTATCTCGCCACC
GAATTCGAGGAAGGGGGCGTATTACAAATTGAAACGGCAGCACGTGTAGCACAACATGATAATCACTGTGCGGATCATTT
CTTGGCATTTTTAATTCATCCAGAAGCACAAGGGCATTTAGTCAAGAATAATGTGATGTTACCGGTGATTAATACCAATA
TTGAACCGCACTTTGATGCCCTTAAAGCCACCCAAATGAACACGAAAGTGCTCGATACCTCAAAAGTAAATGCCGAACAA
GTCAAAAAATGGATTGCTGTTTGGCAAACGACCCTAACCCAATAA

Upstream 100 bases:

>100_bases
TGAGATGATACCCGTGAACCTGATGCAGTTAATACTGACGTAGGAAACTAGCAGTCAATATTGCTGTGTACTTTTTTGCG
TAATTACAAGAGAGGACACA

Downstream 100 bases:

>100_bases
TTGTTTGCCTTGATGTTTAAGCGATTTCGTGCATTCACATACCGTCCCGCCAGTTATCTTGGCGGGATGTTGGTGATTGT
TTTTCTGATCGCTTTTTATG

Product: TbpA

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MSRLKTSFFFTALSTLSLSVFAQTQAVNVYTYDSFTSEWGAGPKVKKAFETHFPQCQVNFTAFGDSGTMFNRLRLEGKKT
KADVVVGLDNYNLEEAEKSGLFVQHKVDLTPLSLPVEWKNQTFLPYDFGQFAFIYDKTKVQQPPKSLKELVERDDLKVIY
QDPRTSSVGRGLLIWMNHVYTENEVAQAWQKLAKHTVTVGKGWSDTYGAFLKGEADVVLSYNTSPLYHMVFEQKDQYLAT
EFEEGGVLQIETAARVAQHDNHCADHFLAFLIHPEAQGHLVKNNVMLPVINTNIEPHFDALKATQMNTKVLDTSKVNAEQ
VKKWIAVWQTTLTQ

Sequences:

>Translated_334_residues
MSRLKTSFFFTALSTLSLSVFAQTQAVNVYTYDSFTSEWGAGPKVKKAFETHFPQCQVNFTAFGDSGTMFNRLRLEGKKT
KADVVVGLDNYNLEEAEKSGLFVQHKVDLTPLSLPVEWKNQTFLPYDFGQFAFIYDKTKVQQPPKSLKELVERDDLKVIY
QDPRTSSVGRGLLIWMNHVYTENEVAQAWQKLAKHTVTVGKGWSDTYGAFLKGEADVVLSYNTSPLYHMVFEQKDQYLAT
EFEEGGVLQIETAARVAQHDNHCADHFLAFLIHPEAQGHLVKNNVMLPVINTNIEPHFDALKATQMNTKVLDTSKVNAEQ
VKKWIAVWQTTLTQ
>Mature_333_residues
SRLKTSFFFTALSTLSLSVFAQTQAVNVYTYDSFTSEWGAGPKVKKAFETHFPQCQVNFTAFGDSGTMFNRLRLEGKKTK
ADVVVGLDNYNLEEAEKSGLFVQHKVDLTPLSLPVEWKNQTFLPYDFGQFAFIYDKTKVQQPPKSLKELVERDDLKVIYQ
DPRTSSVGRGLLIWMNHVYTENEVAQAWQKLAKHTVTVGKGWSDTYGAFLKGEADVVLSYNTSPLYHMVFEQKDQYLATE
FEEGGVLQIETAARVAQHDNHCADHFLAFLIHPEAQGHLVKNNVMLPVINTNIEPHFDALKATQMNTKVLDTSKVNAEQV
KKWIAVWQTTLTQ

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import [H]

COG id: COG4143

COG function: function code H; ABC-type thiamine transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1786255, Length=317, Percent_Identity=49.8422712933754, Blast_Score=304, Evalue=5e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR006061
- InterPro:   IPR005948
- InterPro:   IPR005967 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37900; Mature: 37769

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLKTSFFFTALSTLSLSVFAQTQAVNVYTYDSFTSEWGAGPKVKKAFETHFPQCQVNF
CCCHHHHHHHHHHHHHHEEEEEEEEEEEEEEECCCCHHCCCCCHHHHHHHHCCCEEEEEE
TAFGDSGTMFNRLRLEGKKTKADVVVGLDNYNLEEAEKSGLFVQHKVDLTPLSLPVEWKN
EEECCCCCEEEEEEECCCCCCEEEEEECCCCCHHHHHHCCEEEEEEECCCEEEEEEEECC
QTFLPYDFGQFAFIYDKTKVQQPPKSLKELVERDDLKVIYQDPRTSSVGRGLLIWMNHVY
CEECCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEEEEHHC
TENEVAQAWQKLAKHTVTVGKGWSDTYGAFLKGEADVVLSYNTSPLYHMVFEQKDQYLAT
CCHHHHHHHHHHHHHEEEECCCCCCCCCEEECCCCEEEEEECCCCEEEHHHHCCCCCEEE
EFEEGGVLQIETAARVAQHDNHCADHFLAFLIHPEAQGHLVKNNVMLPVINTNIEPHFDA
ECCCCCEEEEHHHHHHHHCCCHHHHHEEEEEECCCCCCEEEECCEEEEEEECCCCCCHHH
LKATQMNTKVLDTSKVNAEQVKKWIAVWQTTLTQ
HHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SRLKTSFFFTALSTLSLSVFAQTQAVNVYTYDSFTSEWGAGPKVKKAFETHFPQCQVNF
CCHHHHHHHHHHHHHHEEEEEEEEEEEEEEECCCCHHCCCCCHHHHHHHHCCCEEEEEE
TAFGDSGTMFNRLRLEGKKTKADVVVGLDNYNLEEAEKSGLFVQHKVDLTPLSLPVEWKN
EEECCCCCEEEEEEECCCCCCEEEEEECCCCCHHHHHHCCEEEEEEECCCEEEEEEEECC
QTFLPYDFGQFAFIYDKTKVQQPPKSLKELVERDDLKVIYQDPRTSSVGRGLLIWMNHVY
CEECCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEEEEHHC
TENEVAQAWQKLAKHTVTVGKGWSDTYGAFLKGEADVVLSYNTSPLYHMVFEQKDQYLAT
CCHHHHHHHHHHHHHEEEECCCCCCCCCEEECCCCEEEEEECCCCEEEHHHHCCCCCEEE
EFEEGGVLQIETAARVAQHDNHCADHFLAFLIHPEAQGHLVKNNVMLPVINTNIEPHFDA
ECCCCCEEEEHHHHHHHHCCCHHHHHEEEEEECCCCCCEEEECCEEEEEEECCCCCCHHH
LKATQMNTKVLDTSKVNAEQVKKWIAVWQTTLTQ
HHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]