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The map label for this gene is mepA [H]

Identifier: 15602225

GI number: 15602225

Start: 422185

End: 423045

Strand: Direct

Name: mepA [H]

Synonym: PM0360

Alternate gene names: 15602225

Gene position: 422185-423045 (Clockwise)

Preceding gene: 15602224

Following gene: 15602226

Centisome position: 18.7

GC content: 45.18

Gene sequence:

>861_bases
ATGATGAAAAAAAGCGTTAATATCGCGGTTATGTTGACCGCACTTTTCAGCCTGTTGTGTAGCCTTGCTTATGCGTCGCC
ACAAGAATGGCAGAAAATCAAACGCCCGATTCCGGGCAGCCCTGAGCCGATTGGTAGTTATTCCAATGGCTGTATTATTG
GCGCGCAAGCCTTACCCTATAAAGGTGATGGTTATCAAGTGATTCGTAAAAATCGCAATCGTTATTATGGTCACCCCGAT
ATGATTGACTATTTGCAACGTTTGGGCAAAAAAGTCAAAGCCGCAGGGTTACCTACAATGCTGATTGGCGATATTGCTAT
GCCTGGTGGTGGACGTTTTTTGACGGGGCATGCCAGTCACCAAATGGGCTTAGATGCGGATATTTGGTTGCGTATGGGCA
CTATGTCTGATCAAGATGCATTGAATTCTGATGGCAAAGGCTTGTTGGTAGTCGATCGTAAAGCACAGCGTGTAGATGAG
CGAGTTTGGAACAACAATCATGCCTTATTAATTAAGCTGGCAGCAGAAGATCCAAAAGTGACACGTATTTTTGTCAATCC
AGCGATTAAACTCAAATTATGTCAGAGTGCGGGAAATCAGCGAGCATGGTTGCATAAAGTGCGTCCTTGGTTCGGACATG
ATTCTCACTTTCATGTTCGTTTGACTTGTCCAAAGGGGGCAAGTTACTGTGAAAATCAAGCACCAGTGCCTGCAGGGGAT
GGCTGTGGAAGTGAATTATATTCTTGGTTCGAGCCACCGAAACCGACAAAAACACCAAGTGAACCTAAAGCGGTTCCACA
AGCTCCCCCTTTGTGTCAACAGATCTTAAATGCACCCAATCGGAGTGAATGGTTGGAATAA

Upstream 100 bases:

>100_bases
GATCACTTATTAAGACATAAAGCGCAAAATAGATAATGATGAAGTCATGTGCAAAGGATGGCACCAACACGAGGTTTAAT
TGAGGATGACATCCACGAAT

Downstream 100 bases:

>100_bases
GGAAAATAAATGGAAATTGGAATCAATGTGCTTGTACTTCTCTTTGCTGTAGCATTGGTCGCGGGGTTTATTGATGCGAT
TGCGGGTGGCGGTGGATTAA

Product: penicillin-insensitive murein endopeptidase

Products: Split Peptidoglycan Strands [C]

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MMKKSVNIAVMLTALFSLLCSLAYASPQEWQKIKRPIPGSPEPIGSYSNGCIIGAQALPYKGDGYQVIRKNRNRYYGHPD
MIDYLQRLGKKVKAAGLPTMLIGDIAMPGGGRFLTGHASHQMGLDADIWLRMGTMSDQDALNSDGKGLLVVDRKAQRVDE
RVWNNNHALLIKLAAEDPKVTRIFVNPAIKLKLCQSAGNQRAWLHKVRPWFGHDSHFHVRLTCPKGASYCENQAPVPAGD
GCGSELYSWFEPPKPTKTPSEPKAVPQAPPLCQQILNAPNRSEWLE

Sequences:

>Translated_286_residues
MMKKSVNIAVMLTALFSLLCSLAYASPQEWQKIKRPIPGSPEPIGSYSNGCIIGAQALPYKGDGYQVIRKNRNRYYGHPD
MIDYLQRLGKKVKAAGLPTMLIGDIAMPGGGRFLTGHASHQMGLDADIWLRMGTMSDQDALNSDGKGLLVVDRKAQRVDE
RVWNNNHALLIKLAAEDPKVTRIFVNPAIKLKLCQSAGNQRAWLHKVRPWFGHDSHFHVRLTCPKGASYCENQAPVPAGD
GCGSELYSWFEPPKPTKTPSEPKAVPQAPPLCQQILNAPNRSEWLE
>Mature_286_residues
MMKKSVNIAVMLTALFSLLCSLAYASPQEWQKIKRPIPGSPEPIGSYSNGCIIGAQALPYKGDGYQVIRKNRNRYYGHPD
MIDYLQRLGKKVKAAGLPTMLIGDIAMPGGGRFLTGHASHQMGLDADIWLRMGTMSDQDALNSDGKGLLVVDRKAQRVDE
RVWNNNHALLIKLAAEDPKVTRIFVNPAIKLKLCQSAGNQRAWLHKVRPWFGHDSHFHVRLTCPKGASYCENQAPVPAGD
GCGSELYSWFEPPKPTKTPSEPKAVPQAPPLCQQILNAPNRSEWLE

Specific function: Involved in the removal of murein from the sacculus. May also facilitate integration of nascent murein strands into the sacculus by cleaving the peptide bonds between neighboring strands in mature murein [H]

COG id: COG3770

COG function: function code M; Murein endopeptidase

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M74 family [H]

Homologues:

Organism=Escherichia coli, GI1788668, Length=248, Percent_Identity=52.8225806451613, Blast_Score=274, Evalue=6e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009045
- InterPro:   IPR005073 [H]

Pfam domain/function: PF03411 Peptidase_M74 [H]

EC number: 3.4.99.- [C]

Molecular weight: Translated: 31666; Mature: 31666

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKKSVNIAVMLTALFSLLCSLAYASPQEWQKIKRPIPGSPEPIGSYSNGCIIGAQALPY
CCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCC
KGDGYQVIRKNRNRYYGHPDMIDYLQRLGKKVKAAGLPTMLIGDIAMPGGGRFLTGHASH
CCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCEECCCCCEEEECCCCC
QMGLDADIWLRMGTMSDQDALNSDGKGLLVVDRKAQRVDERVWNNNHALLIKLAAEDPKV
CCCCCHHHEEEECCCCCHHHCCCCCCEEEEECCHHHHHHHHHHCCCCEEEEEEECCCCCE
TRIFVNPAIKLKLCQSAGNQRAWLHKVRPWFGHDSHFHVRLTCPKGASYCENQAPVPAGD
EEEEECCCEEEEEECCCCCCCHHHHHCCCCCCCCCEEEEEEECCCCCCHHCCCCCCCCCC
GCGSELYSWFEPPKPTKTPSEPKAVPQAPPLCQQILNAPNRSEWLE
CCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCHHHCCC
>Mature Secondary Structure
MMKKSVNIAVMLTALFSLLCSLAYASPQEWQKIKRPIPGSPEPIGSYSNGCIIGAQALPY
CCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCC
KGDGYQVIRKNRNRYYGHPDMIDYLQRLGKKVKAAGLPTMLIGDIAMPGGGRFLTGHASH
CCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCEECCCCCEEEECCCCC
QMGLDADIWLRMGTMSDQDALNSDGKGLLVVDRKAQRVDERVWNNNHALLIKLAAEDPKV
CCCCCHHHEEEECCCCCHHHCCCCCCEEEEECCHHHHHHHHHHCCCCEEEEEEECCCCCE
TRIFVNPAIKLKLCQSAGNQRAWLHKVRPWFGHDSHFHVRLTCPKGASYCENQAPVPAGD
EEEEECCCEEEEEECCCCCCCHHHHHCCCCCCCCCEEEEEEECCCCCCHHCCCCCCCCCC
GCGSELYSWFEPPKPTKTPSEPKAVPQAPPLCQQILNAPNRSEWLE
CCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond [C]

Specific reaction: Split The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond Connecting Neighboring Peptidoglycan Strands. [C]

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]