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The map label for this gene is cafA [H]

Identifier: 15602207

GI number: 15602207

Start: 403136

End: 404611

Strand: Direct

Name: cafA [H]

Synonym: PM0342

Alternate gene names: 15602207

Gene position: 403136-404611 (Clockwise)

Preceding gene: 15602204

Following gene: 15602208

Centisome position: 17.86

GC content: 41.8

Gene sequence:

>1476_bases
ATGGATTCTGTTGAGCTGTTAGTGAATGTCACACCGAGTGAAACACGGATTGCCTTAGTCGATATGGGGATCTTAAAAGA
GGTACATATTGAGCGTCAGGCGAAACGTGGTATTGTGGGTAATATTTATAAAGGGCGTGTTACAAGAGTATTGCCGGGTA
TGCAATCGGCATTTGTGGATATTGGTTTAGAAAAAGCCGCTTTTTTGCATGCTTCAGATATTGTTTCGCACACTGAATGT
GTGGAAGAAAGCGAGCAAAAGCAGTTTGTGGTGAAAGACATTGCGGAACTGGTACGCGAAGGACAAGATATTGTGGTGCA
AGTGGTGAAAGATCCTTTAGGAACCAAAGGGGCACGGTTAACCACAGATATCACCTTGCCTTCACGTTACTTAGTGTTTA
TGCCTGAAAATAGCCATGTAGGGGTATCACAACGGATTGAAAGTGAAGAAGAACGCGCCAGATTAAAAGAGTTAGTCCTG
CCGCTTTGTGATGAACTTGGTGGTTTTATTATCCGTACCGCAGCCGATGGTGCAACAGAAGAAGAACTTAAACAAGATGC
GGATTTTTTAAAACGTTTATGGCGTAAGGTCTTAGAACGTCGGGCAAAATACCCAACAAAATCCATGTTATATGGTGAGC
TTGCATTAACCCAACGCATTTTACGTGATTTTATCGGGGCGGGATTAGAAAAAATTCGTATTGACTCGAAATTATGTTTT
ACGGAAGTGAAGCAATTTACTGAGGAATTTATGCCGACGTTGGTGGATAAGTTGGTGTTGTATTCGGGGAATCAGCCTTT
GTTTGATGTGTATGGCGTGGAGGCGGCGATTCAAACCGCGTTAAATAAACGGGTTAATTTGAAATCTGGTGGCTATTTAA
TCATTGAGCAAACCGAAGCCATGACCACTATTGATATTAATACGGGGGCATTTGTAGGGCATCGTAACCTCGAGGAAACC
ATTTTTAATACCAATATTGAAGCGACAAAAGCCATTGCCCAGCAGCTTCAATTACGTAATCTCGGTGGGATTATCATTAT
TGATTTTATTGATATGCAAACCGATGATCACCGTGTTCGTGTATTAGAATCCCTGCAAGAAGCACTCTCTAAAGACCGTG
TCAAAACGAGCGTAAATGGCTTTACACAATTAGGTTTGGTAGAAATGACGCGTAAACGAACACGTGAAAGTTTAGAACAT
GTGTTATGTGGCGATTGCCCTGCTTGTGAGGGACGTGGACGTATCAAAACAGTGGAAACCGTCTGCTACGAAATTATGCG
TGAGATTATTCGTGTACACCATTTATTTGCCAGTGAGCAATTTGTGGTCTATGCCTCAAGGGCTGTGGCAGATTATCTCA
TCAATGAAGAATCGCATGGGTTATTAGCTGAGTTAGAAGTCTTTATCGGCAAGCAAGTTCAAATCAAAACAGAAGTGTAT
TATAACCAAGAGCAGTTTGATGTAGTGGTGATGTAA

Upstream 100 bases:

>100_bases
GCTCACATTTTACCTTATTTAGCACTATTATTCTATTTCCCTTTTCGTTAGACTAACTGGCAATTGAATGTCTTGAAAAA
TGATGATGTTAGGTAGAAGT

Downstream 100 bases:

>100_bases
TGAAAAAGGAGCAAGGGCAGACTTGCTCCTTTTGCTTTTGGGTATAAAAGTGCGGTCATTTTTAAAAAAGTTTCGCTAAG
CCAAGCACTTTTGTCTTTCC

Product: ribonuclease G

Products: NA

Alternate protein names: RNase G; Cytoplasmic axial filament protein [H]

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MDSVELLVNVTPSETRIALVDMGILKEVHIERQAKRGIVGNIYKGRVTRVLPGMQSAFVDIGLEKAAFLHASDIVSHTEC
VEESEQKQFVVKDIAELVREGQDIVVQVVKDPLGTKGARLTTDITLPSRYLVFMPENSHVGVSQRIESEEERARLKELVL
PLCDELGGFIIRTAADGATEEELKQDADFLKRLWRKVLERRAKYPTKSMLYGELALTQRILRDFIGAGLEKIRIDSKLCF
TEVKQFTEEFMPTLVDKLVLYSGNQPLFDVYGVEAAIQTALNKRVNLKSGGYLIIEQTEAMTTIDINTGAFVGHRNLEET
IFNTNIEATKAIAQQLQLRNLGGIIIIDFIDMQTDDHRVRVLESLQEALSKDRVKTSVNGFTQLGLVEMTRKRTRESLEH
VLCGDCPACEGRGRIKTVETVCYEIMREIIRVHHLFASEQFVVYASRAVADYLINEESHGLLAELEVFIGKQVQIKTEVY
YNQEQFDVVVM

Sequences:

>Translated_491_residues
MDSVELLVNVTPSETRIALVDMGILKEVHIERQAKRGIVGNIYKGRVTRVLPGMQSAFVDIGLEKAAFLHASDIVSHTEC
VEESEQKQFVVKDIAELVREGQDIVVQVVKDPLGTKGARLTTDITLPSRYLVFMPENSHVGVSQRIESEEERARLKELVL
PLCDELGGFIIRTAADGATEEELKQDADFLKRLWRKVLERRAKYPTKSMLYGELALTQRILRDFIGAGLEKIRIDSKLCF
TEVKQFTEEFMPTLVDKLVLYSGNQPLFDVYGVEAAIQTALNKRVNLKSGGYLIIEQTEAMTTIDINTGAFVGHRNLEET
IFNTNIEATKAIAQQLQLRNLGGIIIIDFIDMQTDDHRVRVLESLQEALSKDRVKTSVNGFTQLGLVEMTRKRTRESLEH
VLCGDCPACEGRGRIKTVETVCYEIMREIIRVHHLFASEQFVVYASRAVADYLINEESHGLLAELEVFIGKQVQIKTEVY
YNQEQFDVVVM
>Mature_491_residues
MDSVELLVNVTPSETRIALVDMGILKEVHIERQAKRGIVGNIYKGRVTRVLPGMQSAFVDIGLEKAAFLHASDIVSHTEC
VEESEQKQFVVKDIAELVREGQDIVVQVVKDPLGTKGARLTTDITLPSRYLVFMPENSHVGVSQRIESEEERARLKELVL
PLCDELGGFIIRTAADGATEEELKQDADFLKRLWRKVLERRAKYPTKSMLYGELALTQRILRDFIGAGLEKIRIDSKLCF
TEVKQFTEEFMPTLVDKLVLYSGNQPLFDVYGVEAAIQTALNKRVNLKSGGYLIIEQTEAMTTIDINTGAFVGHRNLEET
IFNTNIEATKAIAQQLQLRNLGGIIIIDFIDMQTDDHRVRVLESLQEALSKDRVKTSVNGFTQLGLVEMTRKRTRESLEH
VLCGDCPACEGRGRIKTVETVCYEIMREIIRVHHLFASEQFVVYASRAVADYLINEESHGLLAELEVFIGKQVQIKTEVY
YNQEQFDVVVM

Specific function: Involved in the processing of the 5'-end of 16S rRNA. Could be involved in chromosome segregation and cell division. It may be one of the components of the cytoplasmic axial filaments bundles or merely regulate the formation of this structure [H]

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm, cytoskeleton [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI87082235, Length=489, Percent_Identity=71.7791411042945, Blast_Score=713, Evalue=0.0,
Organism=Escherichia coli, GI1787325, Length=463, Percent_Identity=36.5010799136069, Blast_Score=275, Evalue=5e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]

EC number: 3.1.4.- [C]

Molecular weight: Translated: 55497; Mature: 55497

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSVELLVNVTPSETRIALVDMGILKEVHIERQAKRGIVGNIYKGRVTRVLPGMQSAFVD
CCCEEEEEEECCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCHHHHHHH
IGLEKAAFLHASDIVSHTECVEESEQKQFVVKDIAELVREGQDIVVQVVKDPLGTKGARL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEE
TTDITLPSRYLVFMPENSHVGVSQRIESEEERARLKELVLPLCDELGGFIIRTAADGATE
EEEEECCCEEEEEECCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCH
EELKQDADFLKRLWRKVLERRAKYPTKSMLYGELALTQRILRDFIGAGLEKIRIDSKLCF
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHEEECHHHHH
TEVKQFTEEFMPTLVDKLVLYSGNQPLFDVYGVEAAIQTALNKRVNLKSGGYLIIEQTEA
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHCCCEECCCCEEEEEECCC
MTTIDINTGAFVGHRNLEETIFNTNIEATKAIAQQLQLRNLGGIIIIDFIDMQTDDHRVR
EEEEEECCCCEEECCCHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHH
VLESLQEALSKDRVKTSVNGFTQLGLVEMTRKRTRESLEHVLCGDCPACEGRGRIKTVET
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
VCYEIMREIIRVHHLFASEQFVVYASRAVADYLINEESHGLLAELEVFIGKQVQIKTEVY
HHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEEEE
YNQEQFDVVVM
ECCCCEEEEEC
>Mature Secondary Structure
MDSVELLVNVTPSETRIALVDMGILKEVHIERQAKRGIVGNIYKGRVTRVLPGMQSAFVD
CCCEEEEEEECCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCHHHHHHH
IGLEKAAFLHASDIVSHTECVEESEQKQFVVKDIAELVREGQDIVVQVVKDPLGTKGARL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEE
TTDITLPSRYLVFMPENSHVGVSQRIESEEERARLKELVLPLCDELGGFIIRTAADGATE
EEEEECCCEEEEEECCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCH
EELKQDADFLKRLWRKVLERRAKYPTKSMLYGELALTQRILRDFIGAGLEKIRIDSKLCF
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHEEECHHHHH
TEVKQFTEEFMPTLVDKLVLYSGNQPLFDVYGVEAAIQTALNKRVNLKSGGYLIIEQTEA
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHCCCEECCCCEEEEEECCC
MTTIDINTGAFVGHRNLEETIFNTNIEATKAIAQQLQLRNLGGIIIIDFIDMQTDDHRVR
EEEEEECCCCEEECCCHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHH
VLESLQEALSKDRVKTSVNGFTQLGLVEMTRKRTRESLEHVLCGDCPACEGRGRIKTVET
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
VCYEIMREIIRVHHLFASEQFVVYASRAVADYLINEESHGLLAELEVFIGKQVQIKTEVY
HHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEEEE
YNQEQFDVVVM
ECCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]