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The map label for this gene is birA [H]

Identifier: 15602161

GI number: 15602161

Start: 339194

End: 340132

Strand: Direct

Name: birA [H]

Synonym: PM0296

Alternate gene names: 15602161

Gene position: 339194-340132 (Clockwise)

Preceding gene: 15602152

Following gene: 15602163

Centisome position: 15.03

GC content: 39.51

Gene sequence:

>939_bases
ATGCAAAACGTACTTGCATTACTCGCTAGCTATCAACAACTTTCTTTAGCAGAATTGACCGCACTTTTAGGTTATTCAGA
ACAAGATATCTTACACAATATCGAAAAATTGAAACAACAAGGGATACAAATAGAACAGCAAGTCCAACATTTTCGTCTCA
TACCTCAATTAAGTCGCTTAAATGAATCTTATTTGACGCAAGCGTTAGCACCTTACCCACTTTATATTAAGCCGATTATT
AACTCGACCAATCAATTCTTGCTTGACAATATTGCACATTTGGAAAAAGGCGCTATTTGCTTAGCAGAATATCAAACTGC
GGGACGTGGACGACGTGGTCGTCAATGGCTTTCTCCTTTTGCTGGACAAGTGATTATGAGTTTGTATTGGACATGTGACC
AAAAGGTCAATTTAGAAGGCTTGAGTTTAGTGGTGGGCATGGCAATCGCAGAAACACTAAAACAAGCAGGTGCTTTGAAC
ATTGGATTAAAATGGCCAAATGATGTGCTATTACATGGGCGCAAATTAGCAGGGATTTTGGTCGAAATCGCCAATCGCCA
GAATAATCAACATAATCTCGTGATTGGCTTCGGCATTAATTTGTCTTTCCCAAAACAAACACAGCAAATTTATCAACCTT
GGGCAGAGCTGATTGAAGTATTACCAACGATTGATCGGAATAAATTGATCGCAGAATTAGGTAAAAAGTTGATTGCACGT
TTACAACATTTTGAACAAGCAGGAATTGATGAAGCATTTCGCCAAGATTGGCGAGATATGGATGCGTATTTTGGGGAAGA
GGTTCATGTGTTAACGGAAAACCAGAAAATCACAGGTATTGCGCAGGGAATTGATAAACGGGGTTATCTTCAACTGATAA
CGAGCGAGGGAATACAATATTTCAATGGTGGGGAAGTGTCACTCAGGAAAGCCTCTTGA

Upstream 100 bases:

>100_bases
GATTTCGTTGCTGTTTGAAAATGGATTTTTTTATCGATTTTCGAGAAAATCATTGTCTATAATAAGTTGCCTCTCTTTGG
TTATATAAATAAGGATTTTT

Downstream 100 bases:

>100_bases
TACTATCGTTATAAATAAAAGTGCGGTCAAAAAAATGAAAATTTTTAACCGCACTTTATCCTTTACACAACAACTTGTAC
TAATGCTGCAATATCCTCAG

Product: biotin--protein ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MQNVLALLASYQQLSLAELTALLGYSEQDILHNIEKLKQQGIQIEQQVQHFRLIPQLSRLNESYLTQALAPYPLYIKPII
NSTNQFLLDNIAHLEKGAICLAEYQTAGRGRRGRQWLSPFAGQVIMSLYWTCDQKVNLEGLSLVVGMAIAETLKQAGALN
IGLKWPNDVLLHGRKLAGILVEIANRQNNQHNLVIGFGINLSFPKQTQQIYQPWAELIEVLPTIDRNKLIAELGKKLIAR
LQHFEQAGIDEAFRQDWRDMDAYFGEEVHVLTENQKITGIAQGIDKRGYLQLITSEGIQYFNGGEVSLRKAS

Sequences:

>Translated_312_residues
MQNVLALLASYQQLSLAELTALLGYSEQDILHNIEKLKQQGIQIEQQVQHFRLIPQLSRLNESYLTQALAPYPLYIKPII
NSTNQFLLDNIAHLEKGAICLAEYQTAGRGRRGRQWLSPFAGQVIMSLYWTCDQKVNLEGLSLVVGMAIAETLKQAGALN
IGLKWPNDVLLHGRKLAGILVEIANRQNNQHNLVIGFGINLSFPKQTQQIYQPWAELIEVLPTIDRNKLIAELGKKLIAR
LQHFEQAGIDEAFRQDWRDMDAYFGEEVHVLTENQKITGIAQGIDKRGYLQLITSEGIQYFNGGEVSLRKAS
>Mature_312_residues
MQNVLALLASYQQLSLAELTALLGYSEQDILHNIEKLKQQGIQIEQQVQHFRLIPQLSRLNESYLTQALAPYPLYIKPII
NSTNQFLLDNIAHLEKGAICLAEYQTAGRGRRGRQWLSPFAGQVIMSLYWTCDQKVNLEGLSLVVGMAIAETLKQAGALN
IGLKWPNDVLLHGRKLAGILVEIANRQNNQHNLVIGFGINLSFPKQTQQIYQPWAELIEVLPTIDRNKLIAELGKKLIAR
LQHFEQAGIDEAFRQDWRDMDAYFGEEVHVLTENQKITGIAQGIDKRGYLQLITSEGIQYFNGGEVSLRKAS

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=311, Percent_Identity=38.2636655948553, Blast_Score=202, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24644246, Length=231, Percent_Identity=30.3030303030303, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR004409
- InterPro:   IPR003142
- InterPro:   IPR004143
- InterPro:   IPR008988 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 35215; Mature: 35215

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNVLALLASYQQLSLAELTALLGYSEQDILHNIEKLKQQGIQIEQQVQHFRLIPQLSRL
CHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHC
NESYLTQALAPYPLYIKPIINSTNQFLLDNIAHLEKGAICLAEYQTAGRGRRGRQWLSPF
CHHHHHHHHCCCCEEEEHHHCCCHHHHHHHHHHHHCCCEEEEEHHHCCCCCCCHHHHHHH
AGQVIMSLYWTCDQKVNLEGLSLVVGMAIAETLKQAGALNIGLKWPNDVLLHGRKLAGIL
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHCCHHHHHHH
VEIANRQNNQHNLVIGFGINLSFPKQTQQIYQPWAELIEVLPTIDRNKLIAELGKKLIAR
HHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LQHFEQAGIDEAFRQDWRDMDAYFGEEVHVLTENQKITGIAQGIDKRGYLQLITSEGIQY
HHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHCCCCCCCEEEEHHCCCEE
FNGGEVSLRKAS
ECCCCEEEEECC
>Mature Secondary Structure
MQNVLALLASYQQLSLAELTALLGYSEQDILHNIEKLKQQGIQIEQQVQHFRLIPQLSRL
CHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHC
NESYLTQALAPYPLYIKPIINSTNQFLLDNIAHLEKGAICLAEYQTAGRGRRGRQWLSPF
CHHHHHHHHCCCCEEEEHHHCCCHHHHHHHHHHHHCCCEEEEEHHHCCCCCCCHHHHHHH
AGQVIMSLYWTCDQKVNLEGLSLVVGMAIAETLKQAGALNIGLKWPNDVLLHGRKLAGIL
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHCCHHHHHHH
VEIANRQNNQHNLVIGFGINLSFPKQTQQIYQPWAELIEVLPTIDRNKLIAELGKKLIAR
HHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LQHFEQAGIDEAFRQDWRDMDAYFGEEVHVLTENQKITGIAQGIDKRGYLQLITSEGIQY
HHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHCCCCCCCEEEEHHCCCEE
FNGGEVSLRKAS
ECCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]