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The map label for this gene is sucB [H]

Identifier: 15602143

GI number: 15602143

Start: 317118

End: 318332

Strand: Direct

Name: sucB [H]

Synonym: PM0278

Alternate gene names: 15602143

Gene position: 317118-318332 (Clockwise)

Preceding gene: 15602142

Following gene: 15602144

Centisome position: 14.05

GC content: 44.03

Gene sequence:

>1215_bases
ATGAGCAATTTTGAGATTATAACTCCCGATTTACCAGAATCTGTCGCCGATGCAACGGTTGTAACTTGGCATAAGAAAGT
GGGTGATGTCGTAAAACGTGATGAGATTTTAGTCGAAATTGAAACCGATAAAGTGGTACTTGAAGTCCCAGCACAATCTG
ATGGTGTACTTGAAGCGATTATTGAAGCAGAAGGCGCCACCGTTATCAGTAAACAATTATTAGGTAAATTGTCAGCCACA
GCCGTAGCGGGTGGTGTCACTAAAGAAACGGTTGTCACTCAAGAGCCAACGCCGGCGGATCGCCATCATGCGAATTTAAG
TACTGAATCAGTGGGTAGCGACTCGGTGAGCCCGGGGGTACGTCGTTTAATTGCTGAGCATGACTTAAATGCAGAAGACA
TTAAAGGAAGCGGTGTGGGCGGGCGTATTACACGCGAAGATGTGGAGAAAGTTATCGCGCAAAAAGCCAATAAAGCGCCG
AATAAGCCGGCTGAGCCTGCGTTTGTCGTCGGTAATCGTGAAGAAAAACGTGTACCAATGACGCGTTTACGCAAACGTAT
TGCGGAGCGTTTGTTAGAAGCGAAAAACAGCACGGCAATGTTAACCACATTTAACGAAGTGGACATGGCGCCGATTATGA
AATTACGTAAAACTTATGGTGAGAAGTTTGAAAAACAACACGGCACGCGTCTCGGTTTTATGTCGTTCTACATTAAAGCA
GTGGTGGAAGCGCTAAAACGTTATCCAGAAGTCAATGCGTCGATTGATGGTGACGATATCATTTATCATAACTATTTCGA
TATCAGCATCGCGGTTTCGACACCACGTGGTTTAGTTACCCCAGTATTACGCAACTGCGATAAATTAAGTATGGTGGATA
TTGAAAAAGAAATTAAAGCGCTTGCTGATAAGGGGCGTGACGGAAAATTAACAGTTGAAGATTTAACCGGGGGTAACTTT
ACCATTACTAATGGTGGTGTGTTCGGTTCCCTCATGTCTACCCCGATTATCAATCCTCCACAAAGTGCCATTTTAGGCAT
GCATGCCATTAAAGATCGCCCTGTGGCGGTGAATGGCGAAGTGGTGATTCGTCCGATGATGTATCTTGCGCTCTCCTATG
ATCACCGTTTAATTGATGGTCGTGAATCAGTTGGTTTCTTAGTAACGATTAAAGAATTGTTGGAAGATCCAACCCGTTTA
TTGTTAGAAATCTAA

Upstream 100 bases:

>100_bases
CATTAGTGAATGAGGCGTTAGCCTAATGGAAAGTGCGGTCAGGTTTGATGAAAACAGACCGCACTTTTCGCAAAAATAAA
ACTGATAAAAGGAAAGAAAA

Downstream 100 bases:

>100_bases
GCATTCATGGAATAAGTGCGGTCAATTTGGTAAAAAATTTCAAATCAGACCGCACTTTTTATCCCTATCAAGAGAATCAG
TTTAAAGGTAAATATGAGCA

Product: hypothetical protein

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MSNFEIITPDLPESVADATVVTWHKKVGDVVKRDEILVEIETDKVVLEVPAQSDGVLEAIIEAEGATVISKQLLGKLSAT
AVAGGVTKETVVTQEPTPADRHHANLSTESVGSDSVSPGVRRLIAEHDLNAEDIKGSGVGGRITREDVEKVIAQKANKAP
NKPAEPAFVVGNREEKRVPMTRLRKRIAERLLEAKNSTAMLTTFNEVDMAPIMKLRKTYGEKFEKQHGTRLGFMSFYIKA
VVEALKRYPEVNASIDGDDIIYHNYFDISIAVSTPRGLVTPVLRNCDKLSMVDIEKEIKALADKGRDGKLTVEDLTGGNF
TITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPVAVNGEVVIRPMMYLALSYDHRLIDGRESVGFLVTIKELLEDPTRL
LLEI

Sequences:

>Translated_404_residues
MSNFEIITPDLPESVADATVVTWHKKVGDVVKRDEILVEIETDKVVLEVPAQSDGVLEAIIEAEGATVISKQLLGKLSAT
AVAGGVTKETVVTQEPTPADRHHANLSTESVGSDSVSPGVRRLIAEHDLNAEDIKGSGVGGRITREDVEKVIAQKANKAP
NKPAEPAFVVGNREEKRVPMTRLRKRIAERLLEAKNSTAMLTTFNEVDMAPIMKLRKTYGEKFEKQHGTRLGFMSFYIKA
VVEALKRYPEVNASIDGDDIIYHNYFDISIAVSTPRGLVTPVLRNCDKLSMVDIEKEIKALADKGRDGKLTVEDLTGGNF
TITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPVAVNGEVVIRPMMYLALSYDHRLIDGRESVGFLVTIKELLEDPTRL
LLEI
>Mature_403_residues
SNFEIITPDLPESVADATVVTWHKKVGDVVKRDEILVEIETDKVVLEVPAQSDGVLEAIIEAEGATVISKQLLGKLSATA
VAGGVTKETVVTQEPTPADRHHANLSTESVGSDSVSPGVRRLIAEHDLNAEDIKGSGVGGRITREDVEKVIAQKANKAPN
KPAEPAFVVGNREEKRVPMTRLRKRIAERLLEAKNSTAMLTTFNEVDMAPIMKLRKTYGEKFEKQHGTRLGFMSFYIKAV
VEALKRYPEVNASIDGDDIIYHNYFDISIAVSTPRGLVTPVLRNCDKLSMVDIEKEIKALADKGRDGKLTVEDLTGGNFT
ITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPVAVNGEVVIRPMMYLALSYDHRLIDGRESVGFLVTIKELLEDPTRLL
LEI

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=243, Percent_Identity=57.6131687242798, Blast_Score=291, Evalue=5e-79,
Organism=Homo sapiens, GI31711992, Length=439, Percent_Identity=29.3849658314351, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI203098753, Length=457, Percent_Identity=28.6652078774617, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI203098816, Length=457, Percent_Identity=28.6652078774617, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI110671329, Length=420, Percent_Identity=28.5714285714286, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI260898739, Length=168, Percent_Identity=35.7142857142857, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1786946, Length=407, Percent_Identity=73.2186732186732, Blast_Score=597, Evalue=1e-172,
Organism=Escherichia coli, GI1786305, Length=410, Percent_Identity=30.4878048780488, Blast_Score=163, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI25146366, Length=403, Percent_Identity=43.424317617866, Blast_Score=313, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI17560088, Length=435, Percent_Identity=31.264367816092, Blast_Score=175, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17537937, Length=423, Percent_Identity=26.7139479905437, Blast_Score=157, Evalue=9e-39,
Organism=Caenorhabditis elegans, GI17538894, Length=328, Percent_Identity=32.0121951219512, Blast_Score=139, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6320352, Length=401, Percent_Identity=46.3840399002494, Blast_Score=338, Evalue=7e-94,
Organism=Saccharomyces cerevisiae, GI6324258, Length=447, Percent_Identity=26.3982102908277, Blast_Score=146, Evalue=6e-36,
Organism=Drosophila melanogaster, GI24645909, Length=229, Percent_Identity=60.2620087336244, Blast_Score=293, Evalue=2e-79,
Organism=Drosophila melanogaster, GI18859875, Length=419, Percent_Identity=27.9236276849642, Blast_Score=158, Evalue=5e-39,
Organism=Drosophila melanogaster, GI20129315, Length=290, Percent_Identity=33.448275862069, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24582497, Length=290, Percent_Identity=33.448275862069, Blast_Score=147, Evalue=1e-35,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 44214; Mature: 44083

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNFEIITPDLPESVADATVVTWHKKVGDVVKRDEILVEIETDKVVLEVPAQSDGVLEAI
CCCCEEECCCCCHHHHCCHHEEHHHHHHHHHCCCCEEEEEECCEEEEECCCCCCCHHHHH
IEAEGATVISKQLLGKLSATAVAGGVTKETVVTQEPTPADRHHANLSTESVGSDSVSPGV
HHCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHH
RRLIAEHDLNAEDIKGSGVGGRITREDVEKVIAQKANKAPNKPAEPAFVVGNREEKRVPM
HHHHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCH
TRLRKRIAERLLEAKNSTAMLTTFNEVDMAPIMKLRKTYGEKFEKQHGTRLGFMSFYIKA
HHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
VVEALKRYPEVNASIDGDDIIYHNYFDISIAVSTPRGLVTPVLRNCDKLSMVDIEKEIKA
HHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEEECCCHHHHHHHHCCCCCCHHHHHHHHHH
LADKGRDGKLTVEDLTGGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPVAVNGE
HHHCCCCCCEEEEECCCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEECCC
VVIRPMMYLALSYDHRLIDGRESVGFLVTIKELLEDPTRLLLEI
EEEHHHHHHHHHCCCCEECCCCCCCCEEEHHHHHCCHHHHHHCC
>Mature Secondary Structure 
SNFEIITPDLPESVADATVVTWHKKVGDVVKRDEILVEIETDKVVLEVPAQSDGVLEAI
CCCEEECCCCCHHHHCCHHEEHHHHHHHHHCCCCEEEEEECCEEEEECCCCCCCHHHHH
IEAEGATVISKQLLGKLSATAVAGGVTKETVVTQEPTPADRHHANLSTESVGSDSVSPGV
HHCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHH
RRLIAEHDLNAEDIKGSGVGGRITREDVEKVIAQKANKAPNKPAEPAFVVGNREEKRVPM
HHHHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCH
TRLRKRIAERLLEAKNSTAMLTTFNEVDMAPIMKLRKTYGEKFEKQHGTRLGFMSFYIKA
HHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
VVEALKRYPEVNASIDGDDIIYHNYFDISIAVSTPRGLVTPVLRNCDKLSMVDIEKEIKA
HHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEEECCCHHHHHHHHCCCCCCHHHHHHHHHH
LADKGRDGKLTVEDLTGGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPVAVNGE
HHHCCCCCCEEEEECCCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEECCC
VVIRPMMYLALSYDHRLIDGRESVGFLVTIKELLEDPTRLLLEI
EEEHHHHHHHHHCCCCEECCCCCCCCEEEHHHHHCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]