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The map label for this gene is topB

Identifier: 15602072

GI number: 15602072

Start: 231866

End: 233818

Strand: Direct

Name: topB

Synonym: PM0207

Alternate gene names: 15602072

Gene position: 231866-233818 (Clockwise)

Preceding gene: 15602071

Following gene: 15602073

Centisome position: 10.27

GC content: 42.65

Gene sequence:

>1953_bases
ATGCGATTATTTATAGCGGAGAAACCCAGCCTTGCAAGAGCCATTGCGGATGTGTTACCTAAACCTCACCAGCGAGGTGA
CGGCTTTATTAAGTGTGGTGCCGATGATTATGTGACATGGTGCATAGGGCATTTACTCGAACAAGCTGAACCCGATGCCT
ATGATCCCAAATTTAAGCAATGGCGCTTAGAACATCTGCCGATTATTCCTGAAAAATGGCAACTCATTCCCCGTAAAGAT
GTACACAAACAATTAACTATCGTTGAAAAATTGATTCATCAAGCGGATATTTTGATTAACGCAGGCGACCCTGATCGTGA
AGGGCAGTTATTGGTGGATGAAGTGTTTAGCTATGCTAATTTAAGTGTCGATAAACTGAATCAGATTCAGCGTTGCTTAA
TTAGTGATCTGAATCCAAGTGCGGTTGAAAAAGCCGTGAACAAACTACAATCAAATCGCAATTTTATCCCCCTTGCTACT
TCCGCTTTAGCACGAGCTCGGGCAGATTGGCTGTATGGTATTAACATGACACGCGCTTATACCTTGCGAGGTCGGCAAGC
TGGTTATCAAGGTGTGTTGTCAGTGGGACGTGTACAAACCCCTGTGTTGGGCTTGATTGTGCGTCGAGATTTAGAGATCG
AAAACTTCAAACCACAGGACTTTTTTGAAGTACTTGCCCATATTCAAGCGGAAACACCAGAAAAAATCACCGCACTTTCT
GCGCAAGAGAAAGCGAACATTCCACAATTTAAAGCCTTATGGCAACCAAGTAAGGCGTGCGAAGATTATCAGGACGAAGA
AGGACGTGTGCTTTCGTTAGGGTTAGTGGAAAATGTAGTCAAACGGATTGCGCAACAACCTGCAGAGGTGGTGGAGTATG
TAGATAAACGCGAACATGAAAGTGCGCCGTTGCCTTATTCTTTGTCCGCGTTACAAATTGATGCGGCGAAACGTTATGGC
TTATCGGCACAAGAAGTATTGGATATTTGTCAACGTTTGTATGAAACGCACCGTTTGATTACGTATCCGCGTTCGGATTG
CCGTTATTTACCAGAAGAGCATTTCGGGGAACGCACAAAAGTCTTTCAGGCGATTTCTCGCCATATTTCAGACTATCAGC
CTTTGCCTGATATTTTAAATCCAGAGCAAAAAAATCGTTGCTGGAATGATAAAAAAGTAGAGGCACACCACGCCATTATT
CCAACAGCGAAAAATACCCCCGTTAACCTGAATCAGCGTGAATGGCAAATTTACCATTTAATCGCACGGCAGTATTTAAT
GCAATTTTGTCCGGATGCAGAATATCGGAAAAGTAAAATAACCTTAAATATTGCGGGGGGCACCTTTATTGCGCAAGCGC
GTAATTTACAAGTTGCGGGCTGGAAACAACTTTTGGGGAAAGAAGACAGTGATGAGCAGCAAGAACCTTTATTACCTGTC
GTGAAAAAAGGGCAGATTTTATTTTGCGAGAAAGGGGAAATTGTTAGTAAAAAAACACAACCCCCTAAACCCTTTACTGA
TGCGACATTGCTTTCAGCAATGACCGGGATTGCGCGTTTTGTACAAGACAAAGAATTGAAAAAAATTCTGCGGGAAACAG
ATGGGTTAGGGACAGAAGCAACAAGGGCTGGCATTATTGAACTCTTATTTAAGCGGGGTTTTTTATATAAAAAAGGACGC
AATATTCACAGTACTGAAACTGGGCGTATTTTAATTCAAGCCTTACCCGATGTGGCAACACAGCCTGATATGACGGCACA
TTGGGAATCACAATTGACCAGTATTAGTCAGAAAGAAATGAGTTATCAGCAATTTATGTCAACGTTGACGAACTTTTTAC
CTGAGTTGATGCGTTATGTGAACTTCGCCGCGTTACGCCAATTAAGTCAAGTGGAGAAACCTCAGTCTTTTTCGAAAAAG
ATGAGCGCGAAGTCTAAAAAGCGTCCTACTTAG

Upstream 100 bases:

>100_bases
TGCACATGGGATTCCAGTGGGGGGGGAATTAGAAATGGTGGATGGTACAACCTTGACTCATTCCTTTTTAGGACGTCGCC
AACTCGGGTAAAATTCGATA

Downstream 100 bases:

>100_bases
CAAGGTAAAATGCCAGTTAGATAAAAGTTACATCTTTTGGGCTTTGCTCAGGTAAGCGGGGGCTAATTGAGGCTGAATGA
GTGAGGAATTAAGTACTGTT

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III

Number of amino acids: Translated: 650; Mature: 650

Protein sequence:

>650_residues
MRLFIAEKPSLARAIADVLPKPHQRGDGFIKCGADDYVTWCIGHLLEQAEPDAYDPKFKQWRLEHLPIIPEKWQLIPRKD
VHKQLTIVEKLIHQADILINAGDPDREGQLLVDEVFSYANLSVDKLNQIQRCLISDLNPSAVEKAVNKLQSNRNFIPLAT
SALARARADWLYGINMTRAYTLRGRQAGYQGVLSVGRVQTPVLGLIVRRDLEIENFKPQDFFEVLAHIQAETPEKITALS
AQEKANIPQFKALWQPSKACEDYQDEEGRVLSLGLVENVVKRIAQQPAEVVEYVDKREHESAPLPYSLSALQIDAAKRYG
LSAQEVLDICQRLYETHRLITYPRSDCRYLPEEHFGERTKVFQAISRHISDYQPLPDILNPEQKNRCWNDKKVEAHHAII
PTAKNTPVNLNQREWQIYHLIARQYLMQFCPDAEYRKSKITLNIAGGTFIAQARNLQVAGWKQLLGKEDSDEQQEPLLPV
VKKGQILFCEKGEIVSKKTQPPKPFTDATLLSAMTGIARFVQDKELKKILRETDGLGTEATRAGIIELLFKRGFLYKKGR
NIHSTETGRILIQALPDVATQPDMTAHWESQLTSISQKEMSYQQFMSTLTNFLPELMRYVNFAALRQLSQVEKPQSFSKK
MSAKSKKRPT

Sequences:

>Translated_650_residues
MRLFIAEKPSLARAIADVLPKPHQRGDGFIKCGADDYVTWCIGHLLEQAEPDAYDPKFKQWRLEHLPIIPEKWQLIPRKD
VHKQLTIVEKLIHQADILINAGDPDREGQLLVDEVFSYANLSVDKLNQIQRCLISDLNPSAVEKAVNKLQSNRNFIPLAT
SALARARADWLYGINMTRAYTLRGRQAGYQGVLSVGRVQTPVLGLIVRRDLEIENFKPQDFFEVLAHIQAETPEKITALS
AQEKANIPQFKALWQPSKACEDYQDEEGRVLSLGLVENVVKRIAQQPAEVVEYVDKREHESAPLPYSLSALQIDAAKRYG
LSAQEVLDICQRLYETHRLITYPRSDCRYLPEEHFGERTKVFQAISRHISDYQPLPDILNPEQKNRCWNDKKVEAHHAII
PTAKNTPVNLNQREWQIYHLIARQYLMQFCPDAEYRKSKITLNIAGGTFIAQARNLQVAGWKQLLGKEDSDEQQEPLLPV
VKKGQILFCEKGEIVSKKTQPPKPFTDATLLSAMTGIARFVQDKELKKILRETDGLGTEATRAGIIELLFKRGFLYKKGR
NIHSTETGRILIQALPDVATQPDMTAHWESQLTSISQKEMSYQQFMSTLTNFLPELMRYVNFAALRQLSQVEKPQSFSKK
MSAKSKKRPT
>Mature_650_residues
MRLFIAEKPSLARAIADVLPKPHQRGDGFIKCGADDYVTWCIGHLLEQAEPDAYDPKFKQWRLEHLPIIPEKWQLIPRKD
VHKQLTIVEKLIHQADILINAGDPDREGQLLVDEVFSYANLSVDKLNQIQRCLISDLNPSAVEKAVNKLQSNRNFIPLAT
SALARARADWLYGINMTRAYTLRGRQAGYQGVLSVGRVQTPVLGLIVRRDLEIENFKPQDFFEVLAHIQAETPEKITALS
AQEKANIPQFKALWQPSKACEDYQDEEGRVLSLGLVENVVKRIAQQPAEVVEYVDKREHESAPLPYSLSALQIDAAKRYG
LSAQEVLDICQRLYETHRLITYPRSDCRYLPEEHFGERTKVFQAISRHISDYQPLPDILNPEQKNRCWNDKKVEAHHAII
PTAKNTPVNLNQREWQIYHLIARQYLMQFCPDAEYRKSKITLNIAGGTFIAQARNLQVAGWKQLLGKEDSDEQQEPLLPV
VKKGQILFCEKGEIVSKKTQPPKPFTDATLLSAMTGIARFVQDKELKKILRETDGLGTEATRAGIIELLFKRGFLYKKGR
NIHSTETGRILIQALPDVATQPDMTAHWESQLTSISQKEMSYQQFMSTLTNFLPELMRYVNFAALRQLSQVEKPQSFSKK
MSAKSKKRPT

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain

Homologues:

Organism=Homo sapiens, GI4507635, Length=700, Percent_Identity=23, Blast_Score=105, Evalue=2e-22,
Organism=Homo sapiens, GI10835218, Length=621, Percent_Identity=24.4766505636071, Blast_Score=97, Evalue=5e-20,
Organism=Escherichia coli, GI1788061, Length=651, Percent_Identity=64.5161290322581, Blast_Score=863, Evalue=0.0,
Organism=Escherichia coli, GI1787529, Length=561, Percent_Identity=25.4901960784314, Blast_Score=109, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17555378, Length=643, Percent_Identity=22.5505443234837, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24640096, Length=690, Percent_Identity=23.3333333333333, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24585251, Length=592, Percent_Identity=23.8175675675676, Blast_Score=80, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOP3_PASMU (Q9CP53)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245144.1
- ProteinModelPortal:   Q9CP53
- SMR:   Q9CP53
- GeneID:   1243554
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0207
- NMPDR:   fig|272843.1.peg.207
- HOGENOM:   HBG585507
- OMA:   NDVVTWC
- ProtClustDB:   PRK07726
- BioCyc:   PMUL272843:PM0207-MONOMER
- BRENDA:   5.99.1.2
- GO:   GO:0005694
- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171
- Gene3D:   G3DSA:1.10.460.10
- Gene3D:   G3DSA:2.70.20.10
- PANTHER:   PTHR11390
- PRINTS:   PR00417
- SMART:   SM00437
- SMART:   SM00436
- SMART:   SM00493
- TIGRFAMs:   TIGR01056

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; SSF56712 Topo_IA_core

EC number: =5.99.1.2

Molecular weight: Translated: 74197; Mature: 74197

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: ACT_SITE 342-342

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLFIAEKPSLARAIADVLPKPHQRGDGFIKCGADDYVTWCIGHLLEQAEPDAYDPKFKQ
CEEEECCCCHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCCCCHHHH
WRLEHLPIIPEKWQLIPRKDVHKQLTIVEKLIHQADILINAGDPDREGQLLVDEVFSYAN
HHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCHHHHHHHHHC
LSVDKLNQIQRCLISDLNPSAVEKAVNKLQSNRNFIPLATSALARARADWLYGINMTRAY
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHCCCCCEEEE
TLRGRQAGYQGVLSVGRVQTPVLGLIVRRDLEIENFKPQDFFEVLAHIQAETPEKITALS
EECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHC
AQEKANIPQFKALWQPSKACEDYQDEEGRVLSLGLVENVVKRIAQQPAEVVEYVDKREHE
CHHHCCCCHHHHHCCCHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCHHHHHHHHHHHCCC
SAPLPYSLSALQIDAAKRYGLSAQEVLDICQRLYETHRLITYPRSDCRYLPEEHFGERTK
CCCCCCCCHHHEEHHHHHHCCCHHHHHHHHHHHHHHHHEEECCCHHHCCCCHHHCCHHHH
VFQAISRHISDYQPLPDILNPEQKNRCWNDKKVEAHHAIIPTAKNTPVNLNQREWQIYHL
HHHHHHHHHHCCCCCCHHCCCCHHHCCCCCCCCCHHHEEECCCCCCCCCCCHHHHHHHHH
IARQYLMQFCPDAEYRKSKITLNIAGGTFIAQARNLQVAGWKQLLGKEDSDEQQEPLLPV
HHHHHHHHHCCCCCCCCCEEEEEECCCEEEHHHCCCCHHHHHHHHCCCCCCCCCCCCCHH
VKKGQILFCEKGEIVSKKTQPPKPFTDATLLSAMTGIARFVQDKELKKILRETDGLGTEA
HCCCCEEEECCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
TRAGIIELLFKRGFLYKKGRNIHSTETGRILIQALPDVATQPDMTAHWESQLTSISQKEM
HHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
SYQQFMSTLTNFLPELMRYVNFAALRQLSQVEKPQSFSKKMSAKSKKRPT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRLFIAEKPSLARAIADVLPKPHQRGDGFIKCGADDYVTWCIGHLLEQAEPDAYDPKFKQ
CEEEECCCCHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCCCCHHHH
WRLEHLPIIPEKWQLIPRKDVHKQLTIVEKLIHQADILINAGDPDREGQLLVDEVFSYAN
HHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCHHHHHHHHHC
LSVDKLNQIQRCLISDLNPSAVEKAVNKLQSNRNFIPLATSALARARADWLYGINMTRAY
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHCCCCCEEEE
TLRGRQAGYQGVLSVGRVQTPVLGLIVRRDLEIENFKPQDFFEVLAHIQAETPEKITALS
EECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHC
AQEKANIPQFKALWQPSKACEDYQDEEGRVLSLGLVENVVKRIAQQPAEVVEYVDKREHE
CHHHCCCCHHHHHCCCHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCHHHHHHHHHHHCCC
SAPLPYSLSALQIDAAKRYGLSAQEVLDICQRLYETHRLITYPRSDCRYLPEEHFGERTK
CCCCCCCCHHHEEHHHHHHCCCHHHHHHHHHHHHHHHHEEECCCHHHCCCCHHHCCHHHH
VFQAISRHISDYQPLPDILNPEQKNRCWNDKKVEAHHAIIPTAKNTPVNLNQREWQIYHL
HHHHHHHHHHCCCCCCHHCCCCHHHCCCCCCCCCHHHEEECCCCCCCCCCCHHHHHHHHH
IARQYLMQFCPDAEYRKSKITLNIAGGTFIAQARNLQVAGWKQLLGKEDSDEQQEPLLPV
HHHHHHHHHCCCCCCCCCEEEEEECCCEEEHHHCCCCHHHHHHHHCCCCCCCCCCCCCHH
VKKGQILFCEKGEIVSKKTQPPKPFTDATLLSAMTGIARFVQDKELKKILRETDGLGTEA
HCCCCEEEECCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
TRAGIIELLFKRGFLYKKGRNIHSTETGRILIQALPDVATQPDMTAHWESQLTSISQKEM
HHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
SYQQFMSTLTNFLPELMRYVNFAALRQLSQVEKPQSFSKKMSAKSKKRPT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100