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The map label for this gene is pfs

Identifier: 15602059

GI number: 15602059

Start: 217192

End: 217881

Strand: Direct

Name: pfs

Synonym: PM0194

Alternate gene names: 15602059

Gene position: 217192-217881 (Clockwise)

Preceding gene: 15602058

Following gene: 15602061

Centisome position: 9.62

GC content: 44.93

Gene sequence:

>690_bases
ATGAAAATTGGTATTGTGGGTGCGATGGCACAAGAAGTTCAAATGTTGGCAGATTTAATGCAAGATAAACGCGTTACCCA
AGTCGCCAGTTGCACAATTTATGAAGGCATGATTCATGGTAAAGCGGTTGCCTTATTACAATCTGGCATTGGCAAAGTCG
CCGCTGCAATCGGTGCCACTTTATTGTTAGAGATGTGCAAACCTGATTTGGTCATTAATACCGGTTCGGCAGGCGGTGTG
GCGACAGGGCTTAACGTCGGGGATATTGTGATTTCTGATGAAACGGTTTACCACGATGCTGATGTGACGGCATTTGGTTA
TGCTAAAGGACAGTTGCCCGCTTGCCCAGCACGATTCCAATCTGACGAGAAGTTAGTACAACTGGCAGAAAAGATCGCTG
TTCAACAACAGCAACAAGTTAAACGTGGTTTGATTTGTTCTGGGGACAGTTTTATTCAAGGTGGGACACCGTTAGCGCAG
ATTAAAGCAGATTTCCCTACGGTTATGGCTGTAGAAATGGAAGCGACAGCAATTGCTCAAGTATGTCATGCCTTCAATGT
GCCTTTTGTGGTTGTCAGGGCAATTTCAGACAGTGGTGACGGTGAGGCGAGTATGTCTTTTGAGGAATTTTTGCCACTGG
CGGCGAAACAATCTTCAGCAATGGTTTTGGAGATGATTGATAATTTATAG

Upstream 100 bases:

>100_bases
GACGTTATGTCTTAACTGCTAGCACCTTATATAGTGATGATTACAGCTTTGCGGTGTTAGATTTTTTAGTAAGTAAATTA
ATGAAGGAAAAACAAAAATA

Downstream 100 bases:

>100_bases
TGCAGTGATGAATCAAACATCATCGTAAAAGTGCGGTAAAAATAGAGATATTTTTGACCGCACTTTTTGATTTTAGTCGT
GGCTAGGGGGATTATTTACC

Product: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase

Products: NA

Alternate protein names: MTA/SAH nucleosidase; MTAN; 5'-methylthioadenosine nucleosidase; MTA nucleosidase; S-adenosylhomocysteine nucleosidase; AdoHcy nucleosidase; SAH nucleosidase; SRH nucleosidase

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MKIGIVGAMAQEVQMLADLMQDKRVTQVASCTIYEGMIHGKAVALLQSGIGKVAAAIGATLLLEMCKPDLVINTGSAGGV
ATGLNVGDIVISDETVYHDADVTAFGYAKGQLPACPARFQSDEKLVQLAEKIAVQQQQQVKRGLICSGDSFIQGGTPLAQ
IKADFPTVMAVEMEATAIAQVCHAFNVPFVVVRAISDSGDGEASMSFEEFLPLAAKQSSAMVLEMIDNL

Sequences:

>Translated_229_residues
MKIGIVGAMAQEVQMLADLMQDKRVTQVASCTIYEGMIHGKAVALLQSGIGKVAAAIGATLLLEMCKPDLVINTGSAGGV
ATGLNVGDIVISDETVYHDADVTAFGYAKGQLPACPARFQSDEKLVQLAEKIAVQQQQQVKRGLICSGDSFIQGGTPLAQ
IKADFPTVMAVEMEATAIAQVCHAFNVPFVVVRAISDSGDGEASMSFEEFLPLAAKQSSAMVLEMIDNL
>Mature_229_residues
MKIGIVGAMAQEVQMLADLMQDKRVTQVASCTIYEGMIHGKAVALLQSGIGKVAAAIGATLLLEMCKPDLVINTGSAGGV
ATGLNVGDIVISDETVYHDADVTAFGYAKGQLPACPARFQSDEKLVQLAEKIAVQQQQQVKRGLICSGDSFIQGGTPLAQ
IKADFPTVMAVEMEATAIAQVCHAFNVPFVVVRAISDSGDGEASMSFEEFLPLAAKQSSAMVLEMIDNL

Specific function: Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S- adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively

COG id: COG0775

COG function: function code F; Nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/UDP phosphorylase family. MtnN subfamily

Homologues:

Organism=Escherichia coli, GI1786354, Length=229, Percent_Identity=60.6986899563319, Blast_Score=260, Evalue=4e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MTNN_PASMU (Q9CP62)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245131.1
- HSSP:   P0AF12
- ProteinModelPortal:   Q9CP62
- SMR:   Q9CP62
- GeneID:   1243541
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0194
- NMPDR:   fig|272843.1.peg.194
- HOGENOM:   HBG367723
- OMA:   AMEQEVT
- ProtClustDB:   PRK05584
- BioCyc:   PMUL272843:PM0194-MONOMER
- HAMAP:   MF_01684
- InterPro:   IPR010049
- InterPro:   IPR018017
- InterPro:   IPR000845
- PANTHER:   PTHR21234
- TIGRFAMs:   TIGR01704

Pfam domain/function: PF01048 PNP_UDP_1

EC number: =3.2.2.9

Molecular weight: Translated: 24065; Mature: 24065

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: NA

Important sites: ACT_SITE 12-12 BINDING 78-78 BINDING 152-152 BINDING 197-197

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGIVGAMAQEVQMLADLMQDKRVTQVASCTIYEGMIHGKAVALLQSGIGKVAAAIGAT
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLLEMCKPDLVINTGSAGGVATGLNVGDIVISDETVYHDADVTAFGYAKGQLPACPARFQ
HHHHHCCCCEEEECCCCCCEEECCCCCEEEECCCEEEECCCCEEEECCCCCCCCCCHHCC
SDEKLVQLAEKIAVQQQQQVKRGLICSGDSFIQGGTPLAQIKADFPTVMAVEMEATAIAQ
CHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCHHHHCCCCCEEEEEEHHHHHHHH
VCHAFNVPFVVVRAISDSGDGEASMSFEEFLPLAAKQSSAMVLEMIDNL
HHHHCCCCEEEEEEECCCCCCCCCCCHHHHHCHHHCCCHHHHHHHHHCC
>Mature Secondary Structure
MKIGIVGAMAQEVQMLADLMQDKRVTQVASCTIYEGMIHGKAVALLQSGIGKVAAAIGAT
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLLEMCKPDLVINTGSAGGVATGLNVGDIVISDETVYHDADVTAFGYAKGQLPACPARFQ
HHHHHCCCCEEEECCCCCCEEECCCCCEEEECCCEEEECCCCEEEECCCCCCCCCCHHCC
SDEKLVQLAEKIAVQQQQQVKRGLICSGDSFIQGGTPLAQIKADFPTVMAVEMEATAIAQ
CHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCHHHHCCCCCEEEEEEHHHHHHHH
VCHAFNVPFVVVRAISDSGDGEASMSFEEFLPLAAKQSSAMVLEMIDNL
HHHHCCCCEEEEEEECCCCCCCCCCCHHHHHCHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100