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The map label for this gene is topA

Identifier: 15595173

GI number: 15595173

Start: 872586

End: 875132

Strand: Reverse

Name: topA

Synonym: BB0828

Alternate gene names: 15595173

Gene position: 875132-872586 (Counterclockwise)

Preceding gene: 15595177

Following gene: 15595172

Centisome position: 96.09

GC content: 28.62

Gene sequence:

>2547_bases
ATGCAAAATAAAAAGTTGATAATAGTTGAATCGCCAACAAAAGCCAAAACAATAAAGAAGTTTCTCGATGAATCATTTCT
AGTAGAAGCATGCATTGGACATGTAGTAGATCTACCAAACAACGCAAAAGAAATCCCAAAAGAATATAAAAAATACGAAT
GGGCAAATATTTCTATAGATTATAACAATGGATTTAATCCAATTTACATTATTCCCAGCAATAAAAAACCAATTGTATCA
AAACTAAAAAAATTAGTAAAAACAATAAATGAAATATATCTTGCAACCGACCAAGACAGAGAAGGAGAAACTATAGCATT
TCACTTAAAAGAAGTATTAAAAATCAAAAACTACAAACGGATGATATTTCATGAAATCACAGAAACCGCAATAACTGAAT
CACTAAAAAATACTAGAAATATAGACATGAACCTTGTTAATGCCGGGGAAGCTAGAAGAATATTGGACCGACTATACGGG
TATACAATCTCTCCACTACTTTGGAAAAAAGTAGCTTATGGACTTTCTGCTGGGCGAGTACAATCTGTTGGATTAAAATT
ATTAATAGAGAAAGAAAAAACTAGAATAAATTTCAAAAAGGCAAATTATTATTCAATTTTACTTCAATGTAAACACGAGA
AAAAAAACTTGTTGCTTGAAGCAAAATTAGAAGAAATTGACGGCAAAAATATAGCAGAGGGTAAAGACTTTGTAAATGAA
ACTGGAAAACTTAAAAATATTGCCAAAACAACAATAATAACCCAAGATTTAATGATAGAGCTTGAAAAAGAATTAAAAAA
TGGACAAAAAATTGAATTAATTTCAATAGAAACTAAAAAAATAAAAATACCTCCTCCAAAGCCATTTACCACCTCTACAC
TTCAACAAGAAATAAATAAGCGTCTTAAAATTGGAACAAAGCAAATCATGCAACACGCTCAAAAACTTTACGAACACGGA
TACATTACCTATATGAGAACAGACTCTCATAATATTGCTAAAATTGCAAAAGATAAAATAACAAAAATAATAAAAAATAA
ATATGGGAAAGAGTATATAGAGGAAAAAGATAGAATTTATGAAAAAGAAAAAATGGCTCAAAATGCACATGAGGCAATAA
GGCCTTCTGAAATATTTATTCCAAATGAAACCATAGAAATAGAAAGCAAAACCGCTAAAGAAATTTACAAAATAATATGG
GATAGAACCATTATTTCTGGAATGAAAGATGCAATAAAAGAAAATATAAAACTGACTTTTAAATATAAAAACTTAATTTT
CAGATCAAGTTTTACAAAAATAATTTTTGATGGATTTCTTAAACACACTAAAGAACAAGATGAACATCTTAACATAAATT
TTGACTTAATTAAAAAGGGAGATACATTTTCCATAGTTAAAATGAAAACAAGTGAGCACGAAACAAAGGCTCCATTTAGA
TACACAGAAGCGTCTCTTGTGCAAAAAATGGAAAAAGAAGGAATAGGTCGTCCCTCGACCTATTCTACAATTATATCAAC
ACTTTTAGAAAGAGAATATGCATTCAAACTTAACAACACATTAATGCCAACTATAAAAGGCGCTGCTGTAATAAATCTTC
TTGAGAAATATTTTCCAGTACTCATTGAACTAAATTTCACCTCTAATATGGAAGAAAAATTAGACAAAATAGCAATAGGA
AAACTAGATAAAATAAAATATCTAAGTAAATTTTATAATGGCAAAAAAGGACTAAAAGATACAGTAATGCAACTAGAGCC
TAAAATTGATTCCTCTGAATTTAGAACCGTTATTGAAAGTCAAAAAATAGAAAATAAAAATAGCATTAATTACACAATAA
ACATTGGTAAATATGGGCCTTATTTGATATTCAAAGGACATAATTACTCAATTAATGCAAAAACTCCATTAGAAAATTTG
TACAAAAAAGATGAAATAGAAAAAATAATAAATGAAAAAGAGCTAAAACCCAATATACTTGGGGTTGATCCTTTAACAGG
ACTTAATGTGATCTTTAAAAATACAATTTACGGAAACATTGTTCAACTTGGAGAAGATACCCATGCCCCTCAAGAATATA
CAAAAAAAGGAAAACCTAAAAAATTAAAAATAATAAAAGCAAAAAAAGCATCAACTAAAAAAATTGACCCTGAAAACATA
ACATTGGAGCTTGCTTTAAAATTGCTCTCACTGCCAAAACCAATTGGCAAACATCCCCAAACCAATGAACAAATCATTGC
TGCAACTGGTGTTTTTGGGGATTATATTAAAACTGAAAGCGGAAGCATTGCTTGCTCGCTAAAAAAAGATTTAAAAGCAT
ATGACATAACACTAGACAAGGCCATCAGCCTACTCAACGAAAGAGCCAATAAAGTGGGTATAATCGTTAAAACAATCACA
TTTTCTAAAAACAAAATTGGCAACAAAATATATATTTACAAAAAAAACGACAAATTTTATGCTAAAATTAAAAGAAAGAA
GATTGATTTACCTGATAACATTAATCTTGAAGAAATAAATGAGAAATATGTATTCAGCTTGTTATAA

Upstream 100 bases:

>100_bases
GTATGCAGAATTTTATAATTGCTCACAAAATACTCTCACTTTTTTTAATTCTTAAATTATATTTATATATTATAATACAA
TATATAAACATAGGGAATTT

Downstream 100 bases:

>100_bases
ATATGAATGATTTCAAACTCCCAATTTATAAATACAAAGATGAATTAATTAAAGTACTAAAAAACCACAATGTTTTAATT
GTAGAAAGTCCAACAGGTAG

Product: DNA topoisomerase I subunit A

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme

Number of amino acids: Translated: 848; Mature: 848

Protein sequence:

>848_residues
MQNKKLIIVESPTKAKTIKKFLDESFLVEACIGHVVDLPNNAKEIPKEYKKYEWANISIDYNNGFNPIYIIPSNKKPIVS
KLKKLVKTINEIYLATDQDREGETIAFHLKEVLKIKNYKRMIFHEITETAITESLKNTRNIDMNLVNAGEARRILDRLYG
YTISPLLWKKVAYGLSAGRVQSVGLKLLIEKEKTRINFKKANYYSILLQCKHEKKNLLLEAKLEEIDGKNIAEGKDFVNE
TGKLKNIAKTTIITQDLMIELEKELKNGQKIELISIETKKIKIPPPKPFTTSTLQQEINKRLKIGTKQIMQHAQKLYEHG
YITYMRTDSHNIAKIAKDKITKIIKNKYGKEYIEEKDRIYEKEKMAQNAHEAIRPSEIFIPNETIEIESKTAKEIYKIIW
DRTIISGMKDAIKENIKLTFKYKNLIFRSSFTKIIFDGFLKHTKEQDEHLNINFDLIKKGDTFSIVKMKTSEHETKAPFR
YTEASLVQKMEKEGIGRPSTYSTIISTLLEREYAFKLNNTLMPTIKGAAVINLLEKYFPVLIELNFTSNMEEKLDKIAIG
KLDKIKYLSKFYNGKKGLKDTVMQLEPKIDSSEFRTVIESQKIENKNSINYTINIGKYGPYLIFKGHNYSINAKTPLENL
YKKDEIEKIINEKELKPNILGVDPLTGLNVIFKNTIYGNIVQLGEDTHAPQEYTKKGKPKKLKIIKAKKASTKKIDPENI
TLELALKLLSLPKPIGKHPQTNEQIIAATGVFGDYIKTESGSIACSLKKDLKAYDITLDKAISLLNERANKVGIIVKTIT
FSKNKIGNKIYIYKKNDKFYAKIKRKKIDLPDNINLEEINEKYVFSLL

Sequences:

>Translated_848_residues
MQNKKLIIVESPTKAKTIKKFLDESFLVEACIGHVVDLPNNAKEIPKEYKKYEWANISIDYNNGFNPIYIIPSNKKPIVS
KLKKLVKTINEIYLATDQDREGETIAFHLKEVLKIKNYKRMIFHEITETAITESLKNTRNIDMNLVNAGEARRILDRLYG
YTISPLLWKKVAYGLSAGRVQSVGLKLLIEKEKTRINFKKANYYSILLQCKHEKKNLLLEAKLEEIDGKNIAEGKDFVNE
TGKLKNIAKTTIITQDLMIELEKELKNGQKIELISIETKKIKIPPPKPFTTSTLQQEINKRLKIGTKQIMQHAQKLYEHG
YITYMRTDSHNIAKIAKDKITKIIKNKYGKEYIEEKDRIYEKEKMAQNAHEAIRPSEIFIPNETIEIESKTAKEIYKIIW
DRTIISGMKDAIKENIKLTFKYKNLIFRSSFTKIIFDGFLKHTKEQDEHLNINFDLIKKGDTFSIVKMKTSEHETKAPFR
YTEASLVQKMEKEGIGRPSTYSTIISTLLEREYAFKLNNTLMPTIKGAAVINLLEKYFPVLIELNFTSNMEEKLDKIAIG
KLDKIKYLSKFYNGKKGLKDTVMQLEPKIDSSEFRTVIESQKIENKNSINYTINIGKYGPYLIFKGHNYSINAKTPLENL
YKKDEIEKIINEKELKPNILGVDPLTGLNVIFKNTIYGNIVQLGEDTHAPQEYTKKGKPKKLKIIKAKKASTKKIDPENI
TLELALKLLSLPKPIGKHPQTNEQIIAATGVFGDYIKTESGSIACSLKKDLKAYDITLDKAISLLNERANKVGIIVKTIT
FSKNKIGNKIYIYKKNDKFYAKIKRKKIDLPDNINLEEINEKYVFSLL
>Mature_848_residues
MQNKKLIIVESPTKAKTIKKFLDESFLVEACIGHVVDLPNNAKEIPKEYKKYEWANISIDYNNGFNPIYIIPSNKKPIVS
KLKKLVKTINEIYLATDQDREGETIAFHLKEVLKIKNYKRMIFHEITETAITESLKNTRNIDMNLVNAGEARRILDRLYG
YTISPLLWKKVAYGLSAGRVQSVGLKLLIEKEKTRINFKKANYYSILLQCKHEKKNLLLEAKLEEIDGKNIAEGKDFVNE
TGKLKNIAKTTIITQDLMIELEKELKNGQKIELISIETKKIKIPPPKPFTTSTLQQEINKRLKIGTKQIMQHAQKLYEHG
YITYMRTDSHNIAKIAKDKITKIIKNKYGKEYIEEKDRIYEKEKMAQNAHEAIRPSEIFIPNETIEIESKTAKEIYKIIW
DRTIISGMKDAIKENIKLTFKYKNLIFRSSFTKIIFDGFLKHTKEQDEHLNINFDLIKKGDTFSIVKMKTSEHETKAPFR
YTEASLVQKMEKEGIGRPSTYSTIISTLLEREYAFKLNNTLMPTIKGAAVINLLEKYFPVLIELNFTSNMEEKLDKIAIG
KLDKIKYLSKFYNGKKGLKDTVMQLEPKIDSSEFRTVIESQKIENKNSINYTINIGKYGPYLIFKGHNYSINAKTPLENL
YKKDEIEKIINEKELKPNILGVDPLTGLNVIFKNTIYGNIVQLGEDTHAPQEYTKKGKPKKLKIIKAKKASTKKIDPENI
TLELALKLLSLPKPIGKHPQTNEQIIAATGVFGDYIKTESGSIACSLKKDLKAYDITLDKAISLLNERANKVGIIVKTIT
FSKNKIGNKIYIYKKNDKFYAKIKRKKIDLPDNINLEEINEKYVFSLL

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain

Homologues:

Organism=Homo sapiens, GI4507635, Length=504, Percent_Identity=25, Blast_Score=100, Evalue=9e-21,
Organism=Escherichia coli, GI1787529, Length=591, Percent_Identity=35.0253807106599, Blast_Score=348, Evalue=6e-97,
Organism=Caenorhabditis elegans, GI32563869, Length=565, Percent_Identity=22.4778761061947, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17555378, Length=524, Percent_Identity=21.7557251908397, Blast_Score=94, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOP1_BORBU (O51768)

Other databases:

- EMBL:   AE000783
- PIR:   C70203
- RefSeq:   NP_212962.1
- ProteinModelPortal:   O51768
- SMR:   O51768
- EnsemblBacteria:   EBBORT00000008495
- GeneID:   1195686
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0828
- NMPDR:   fig|224326.1.peg.1212
- TIGR:   BB_0828
- GeneTree:   EBGT00050000007103
- HOGENOM:   HBG512935
- OMA:   KKVMPRL
- ProtClustDB:   CLSK507867
- BioCyc:   BBUR224326:BB_0828-MONOMER
- BRENDA:   5.99.1.2
- GO:   GO:0005694
- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013826
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005733
- InterPro:   IPR006171
- Gene3D:   G3DSA:1.10.460.10
- Gene3D:   G3DSA:1.10.290.10
- PANTHER:   PTHR11390
- PRINTS:   PR00417
- SMART:   SM00437
- SMART:   SM00436
- SMART:   SM00493
- TIGRFAMs:   TIGR01051

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; SSF56712 Topo_IA_core

EC number: =5.99.1.2

Molecular weight: Translated: 97664; Mature: 97664

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: ACT_SITE 324-324

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNKKLIIVESPTKAKTIKKFLDESFLVEACIGHVVDLPNNAKEIPKEYKKYEWANISID
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEEEEEEE
YNNGFNPIYIIPSNKKPIVSKLKKLVKTINEIYLATDQDREGETIAFHLKEVLKIKNYKR
ECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHH
MIFHEITETAITESLKNTRNIDMNLVNAGEARRILDRLYGYTISPLLWKKVAYGLSAGRV
HHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCH
QSVGLKLLIEKEKTRINFKKANYYSILLQCKHEKKNLLLEAKLEEIDGKNIAEGKDFVNE
HHCCEEEEEEECHHEEEEEECCEEEEEEEECCCCCCEEEEEHHHHCCCCCCCCCHHHHHC
TGKLKNIAKTTIITQDLMIELEKELKNGQKIELISIETKKIKIPPPKPFTTSTLQQEINK
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEECCCCCCCCHHHHHHHHHH
RLKIGTKQIMQHAQKLYEHGYITYMRTDSHNIAKIAKDKITKIIKNKYGKEYIEEKDRIY
HHCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
EKEKMAQNAHEAIRPSEIFIPNETIEIESKTAKEIYKIIWDRTIISGMKDAIKENIKLTF
HHHHHHHHHHHHCCCCEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
KYKNLIFRSSFTKIIFDGFLKHTKEQDEHLNINFDLIKKGDTFSIVKMKTSEHETKAPFR
EEHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCEEEEEEEECCCCCCCCCCE
YTEASLVQKMEKEGIGRPSTYSTIISTLLEREYAFKLNNTLMPTIKGAAVINLLEKYFPV
EHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHCCE
LIELNFTSNMEEKLDKIAIGKLDKIKYLSKFYNGKKGLKDTVMQLEPKIDSSEFRTVIES
EEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHH
QKIENKNSINYTINIGKYGPYLIFKGHNYSINAKTPLENLYKKDEIEKIINEKELKPNIL
HHCCCCCCEEEEEEECCCCCEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEE
GVDPLTGLNVIFKNTIYGNIVQLGEDTHAPQEYTKKGKPKKLKIIKAKKASTKKIDPENI
CCCCCCCCHHHHHCCHHCCCEEECCCCCCCHHHHHCCCCCEEEEEEECCCCCCCCCCCCC
TLELALKLLSLPKPIGKHPQTNEQIIAATGVFGDYIKTESGSIACSLKKDLKAYDITLDK
HHHHHHHHHHCCCCCCCCCCCCCEEEEEECCHHHHHCCCCCCEEEEEHHCCHHHEEEHHH
AISLLNERANKVGIIVKTITFSKNKIGNKIYIYKKNDKFYAKIKRKKIDLPDNINLEEIN
HHHHHHHCCCCEEEEEEEEEECCCCCCCEEEEEECCCCEEEEEEHHCCCCCCCCCHHHHC
EKYVFSLL
CHHHEECC
>Mature Secondary Structure
MQNKKLIIVESPTKAKTIKKFLDESFLVEACIGHVVDLPNNAKEIPKEYKKYEWANISID
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEEEEEEE
YNNGFNPIYIIPSNKKPIVSKLKKLVKTINEIYLATDQDREGETIAFHLKEVLKIKNYKR
ECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHH
MIFHEITETAITESLKNTRNIDMNLVNAGEARRILDRLYGYTISPLLWKKVAYGLSAGRV
HHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCH
QSVGLKLLIEKEKTRINFKKANYYSILLQCKHEKKNLLLEAKLEEIDGKNIAEGKDFVNE
HHCCEEEEEEECHHEEEEEECCEEEEEEEECCCCCCEEEEEHHHHCCCCCCCCCHHHHHC
TGKLKNIAKTTIITQDLMIELEKELKNGQKIELISIETKKIKIPPPKPFTTSTLQQEINK
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEECCCCCCCCHHHHHHHHHH
RLKIGTKQIMQHAQKLYEHGYITYMRTDSHNIAKIAKDKITKIIKNKYGKEYIEEKDRIY
HHCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
EKEKMAQNAHEAIRPSEIFIPNETIEIESKTAKEIYKIIWDRTIISGMKDAIKENIKLTF
HHHHHHHHHHHHCCCCEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
KYKNLIFRSSFTKIIFDGFLKHTKEQDEHLNINFDLIKKGDTFSIVKMKTSEHETKAPFR
EEHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCEEEEEEEECCCCCCCCCCE
YTEASLVQKMEKEGIGRPSTYSTIISTLLEREYAFKLNNTLMPTIKGAAVINLLEKYFPV
EHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHCCE
LIELNFTSNMEEKLDKIAIGKLDKIKYLSKFYNGKKGLKDTVMQLEPKIDSSEFRTVIES
EEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHH
QKIENKNSINYTINIGKYGPYLIFKGHNYSINAKTPLENLYKKDEIEKIINEKELKPNIL
HHCCCCCCEEEEEEECCCCCEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEE
GVDPLTGLNVIFKNTIYGNIVQLGEDTHAPQEYTKKGKPKKLKIIKAKKASTKKIDPENI
CCCCCCCCHHHHHCCHHCCCEEECCCCCCCHHHHHCCCCCEEEEEEECCCCCCCCCCCCC
TLELALKLLSLPKPIGKHPQTNEQIIAATGVFGDYIKTESGSIACSLKKDLKAYDITLDK
HHHHHHHHHHCCCCCCCCCCCCCEEEEEECCHHHHHCCCCCCEEEEEHHCCHHHEEEHHH
AISLLNERANKVGIIVKTITFSKNKIGNKIYIYKKNDKFYAKIKRKKIDLPDNINLEEIN
HHHHHHHCCCCEEEEEEEEEECCCCCCCEEEEEECCCCEEEEEEHHCCCCCCCCCHHHHC
EKYVFSLL
CHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685