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The map label for this gene is nth [H]

Identifier: 15595090

GI number: 15595090

Start: 789270

End: 789938

Strand: Direct

Name: nth [H]

Synonym: BB0745

Alternate gene names: 15595090

Gene position: 789270-789938 (Clockwise)

Preceding gene: 15595089

Following gene: 15595094

Centisome position: 86.66

GC content: 25.26

Gene sequence:

>669_bases
TTGTATATTTTTTATTTTTGTTTTGTGTTTATAATGTTTTATAATTTTTTTATGATTAATCTTGATTTGATTGTTGATGA
AACTTTATTTAGATATCCTGATGTTAAACCTTTTTTAAATTATAAAAACAATTATGAACTTTTAATAATGGTTATTTTAA
GTGCAAGAACAACAGATAATTTGGTGAATAAAATTTCTCCATACCTTTTTGAAAGGTACGAGAATTTTGAAAGTTTATCA
AGAGCAAATGTGAGAGATGTTGAAAAATTAATTTATAAGACTGGTTTTTATTCAAGAAAGGCTAAAAATATTGTGAATTG
CTCTATTGATATTTTAGAAAAATTTAATGGTGTTATTCCAAATAATATTTTTGATCTTATTAAGCTACCTGGAGTAGGTC
GAAAAACGGCAAATGTTATTCTTGGATCTGTTTACAATAAACCCGCAATTATTGTAGATACTCATTTTAGTAGAGTTATT
ACAAGACATGCTCTTTCTTTGGAAAGTTCTCCTATTAAGATTGAATTGGATCTAAAAAGAAGAATAAAGCCTTGTAAGCA
GTATAGATTTTCTATGGCTATTAATAAGCATGCAAGAGAAATTTGTACTTCTAGAAATGTAAATTGTGATAATTGTTTTT
TAGAAAAATTTGCTCCAAGAGTTTGTTAA

Upstream 100 bases:

>100_bases
CTGTTGCTCTATCCAATTGAGCTATTGGTGCATTTAAAATTCTAATGATGAGTATATCATTTTGAAAAATCCTTGTCAAT
AAAGTCTAGATACCTTTACT

Downstream 100 bases:

>100_bases
TTTTTTATTTAAGATGATATTAAATGTTTCATTTTTTTTCTTAGCTTAAAATTTATAATTGCTAGTATTATGTTTATGCC
TATTAATATGAGTAAGGGAT

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MYIFYFCFVFIMFYNFFMINLDLIVDETLFRYPDVKPFLNYKNNYELLIMVILSARTTDNLVNKISPYLFERYENFESLS
RANVRDVEKLIYKTGFYSRKAKNIVNCSIDILEKFNGVIPNNIFDLIKLPGVGRKTANVILGSVYNKPAIIVDTHFSRVI
TRHALSLESSPIKIELDLKRRIKPCKQYRFSMAINKHAREICTSRNVNCDNCFLEKFAPRVC

Sequences:

>Translated_222_residues
MYIFYFCFVFIMFYNFFMINLDLIVDETLFRYPDVKPFLNYKNNYELLIMVILSARTTDNLVNKISPYLFERYENFESLS
RANVRDVEKLIYKTGFYSRKAKNIVNCSIDILEKFNGVIPNNIFDLIKLPGVGRKTANVILGSVYNKPAIIVDTHFSRVI
TRHALSLESSPIKIELDLKRRIKPCKQYRFSMAINKHAREICTSRNVNCDNCFLEKFAPRVC
>Mature_222_residues
MYIFYFCFVFIMFYNFFMINLDLIVDETLFRYPDVKPFLNYKNNYELLIMVILSARTTDNLVNKISPYLFERYENFESLS
RANVRDVEKLIYKTGFYSRKAKNIVNCSIDILEKFNGVIPNNIFDLIKLPGVGRKTANVILGSVYNKPAIIVDTHFSRVI
TRHALSLESSPIKIELDLKRRIKPCKQYRFSMAINKHAREICTSRNVNCDNCFLEKFAPRVC

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Escherichia coli, GI1787920, Length=185, Percent_Identity=33.5135135135135, Blast_Score=104, Evalue=6e-24,
Organism=Drosophila melanogaster, GI45550361, Length=181, Percent_Identity=26.5193370165746, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 26110; Mature: 26110

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYIFYFCFVFIMFYNFFMINLDLIVDETLFRYPDVKPFLNYKNNYELLIMVILSARTTDN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCEEEEEEEHHCCCHHH
LVNKISPYLFERYENFESLSRANVRDVEKLIYKTGFYSRKAKNIVNCSIDILEKFNGVIP
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHCHHHHHHHHHHHHCCCCCC
NNIFDLIKLPGVGRKTANVILGSVYNKPAIIVDTHFSRVITRHALSLESSPIKIELDLKR
CHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHCCCCCEEEEEEHHH
RIKPCKQYRFSMAINKHAREICTSRNVNCDNCFLEKFAPRVC
HCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MYIFYFCFVFIMFYNFFMINLDLIVDETLFRYPDVKPFLNYKNNYELLIMVILSARTTDN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCEEEEEEEHHCCCHHH
LVNKISPYLFERYENFESLSRANVRDVEKLIYKTGFYSRKAKNIVNCSIDILEKFNGVIP
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHCHHHHHHHHHHHHCCCCCC
NNIFDLIKLPGVGRKTANVILGSVYNKPAIIVDTHFSRVITRHALSLESSPIKIELDLKR
CHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHCCCCCEEEEEEHHH
RIKPCKQYRFSMAINKHAREICTSRNVNCDNCFLEKFAPRVC
HCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]