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The map label for this gene is dus [H]

Identifier: 15595082

GI number: 15595082

Start: 777747

End: 778754

Strand: Direct

Name: dus [H]

Synonym: BB0737

Alternate gene names: 15595082

Gene position: 777747-778754 (Clockwise)

Preceding gene: 15595081

Following gene: 15595083

Centisome position: 85.4

GC content: 28.27

Gene sequence:

>1008_bases
TTGTTATGTAGTTATTTTATGAAGTTTTTATTTGATATTCCTCTTCCAATTATGATTTTAGCTCCAATGGAAGATGTTAC
CGATACTGTTTTTAGAAATTTAATTCATTTAATAGGATCTGCAGAAGGAGAACCTCATATTTATTTTACCGAATTTATTT
CTACAAAAGGAATTTTAAATGGATCAAAACAATCTGTTCAACATGTTTTTTTAAAACCCAATGAACTTAATAGGCCTTTA
ATTGCTCAGATTTGGGGCAATGTTCCTGAGCAATTTTATAGAGCAATAGAAATATTAGGGAGCATGGGGTTTTGGGGAAT
TGATATTAATATGGGTTGTCCTAAGAGTAAAATAATTAAAAAAGGAGTTTGTTCAGCTTTAATTAATAATAAATCTTTAG
CTAAAGAGATAATTCTTGCAAGCAAAGAAGCTTGTGCGAGATTTAATTTGCCTCTTAGTGTTAAGACCAGGCATGGATTT
TCATATCCAGAAGTTGATGATTGGCTGGGGTTTTTGTTAGGCCTTGAAATTGATATGTTAACGGTTTATCCAAGACTTGC
TGTTAATCAGAGCAAAGGTCCTGTTAATTTTGATATTTTTTATGAACTTGTTAAATTACGAAATAATCTTAGTCCTTCTA
CTCTGATTATTGGAAATGGGGATGTTTTGAGTCTCAAGGAAGCTAGATATTATGTTGATAAATATTTAATTGATGGAATT
ATGTTTGGTCGTGGAATTTTTGATAATTTGAATTTATTTAAGCCCTTTTCAAAACACTTTTTAGATAGTAATTTAAATTT
TAGGTTAAATATATTAAAATTCCATATAAAGGATTTTCATGCTACTTGGGGACTTGAAAAAGATTTTAATAAACTTAAAA
AATATTTTAAGATATATTTTACTGAGAAGGAAAGACAGAGTAAGTATTTCTCAAATCTTATAAATTCAAAAACTTATGAA
GAGCTTTTTGAAAATTTAAAAGTTATTGACATGGTAGGAGATTTTTAA

Upstream 100 bases:

>100_bases
GTCCTTTTGTGGTATTTCTTTATTTTAAAAATTTCAATGTTATTTTTGGAAATTTAAATTTATTAAACGACTGTTTTACT
TTGTTTAAATTTGTATTTTA

Downstream 100 bases:

>100_bases
ACGATGAATTGTAGACCTCTTGAAAAATATGATCCTAAGGCATTTGAAGATGAAATTTACACTAAGTGGCTTAAAAACAA
TGTTTTTTTGCCAGATAATT

Product: histidine phosphokinase/phophatase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MLCSYFMKFLFDIPLPIMILAPMEDVTDTVFRNLIHLIGSAEGEPHIYFTEFISTKGILNGSKQSVQHVFLKPNELNRPL
IAQIWGNVPEQFYRAIEILGSMGFWGIDINMGCPKSKIIKKGVCSALINNKSLAKEIILASKEACARFNLPLSVKTRHGF
SYPEVDDWLGFLLGLEIDMLTVYPRLAVNQSKGPVNFDIFYELVKLRNNLSPSTLIIGNGDVLSLKEARYYVDKYLIDGI
MFGRGIFDNLNLFKPFSKHFLDSNLNFRLNILKFHIKDFHATWGLEKDFNKLKKYFKIYFTEKERQSKYFSNLINSKTYE
ELFENLKVIDMVGDF

Sequences:

>Translated_335_residues
MLCSYFMKFLFDIPLPIMILAPMEDVTDTVFRNLIHLIGSAEGEPHIYFTEFISTKGILNGSKQSVQHVFLKPNELNRPL
IAQIWGNVPEQFYRAIEILGSMGFWGIDINMGCPKSKIIKKGVCSALINNKSLAKEIILASKEACARFNLPLSVKTRHGF
SYPEVDDWLGFLLGLEIDMLTVYPRLAVNQSKGPVNFDIFYELVKLRNNLSPSTLIIGNGDVLSLKEARYYVDKYLIDGI
MFGRGIFDNLNLFKPFSKHFLDSNLNFRLNILKFHIKDFHATWGLEKDFNKLKKYFKIYFTEKERQSKYFSNLINSKTYE
ELFENLKVIDMVGDF
>Mature_335_residues
MLCSYFMKFLFDIPLPIMILAPMEDVTDTVFRNLIHLIGSAEGEPHIYFTEFISTKGILNGSKQSVQHVFLKPNELNRPL
IAQIWGNVPEQFYRAIEILGSMGFWGIDINMGCPKSKIIKKGVCSALINNKSLAKEIILASKEACARFNLPLSVKTRHGF
SYPEVDDWLGFLLGLEIDMLTVYPRLAVNQSKGPVNFDIFYELVKLRNNLSPSTLIIGNGDVLSLKEARYYVDKYLIDGI
MFGRGIFDNLNLFKPFSKHFLDSNLNFRLNILKFHIKDFHATWGLEKDFNKLKKYFKIYFTEKERQSKYFSNLINSKTYE
ELFENLKVIDMVGDF

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Escherichia coli, GI1789660, Length=323, Percent_Identity=25.077399380805, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI145693211, Length=258, Percent_Identity=24.031007751938, Blast_Score=77, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323437, Length=243, Percent_Identity=24.6913580246914, Blast_Score=66, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 38668; Mature: 38668

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLCSYFMKFLFDIPLPIMILAPMEDVTDTVFRNLIHLIGSAEGEPHIYFTEFISTKGILN
CCHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHCCCCC
GSKQSVQHVFLKPNELNRPLIAQIWGNVPEQFYRAIEILGSMGFWGIDINMGCPKSKIIK
CCHHHHHEEEECCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHH
KGVCSALINNKSLAKEIILASKEACARFNLPLSVKTRHGFSYPEVDDWLGFLLGLEIDML
HHHHHHHHCCHHHHHHHHHHCHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCCHHHH
TVYPRLAVNQSKGPVNFDIFYELVKLRNNLSPSTLIIGNGDVLSLKEARYYVDKYLIDGI
HHCCHHEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCEEEHHHHHHHHHHHHHHHH
MFGRGIFDNLNLFKPFSKHFLDSNLNFRLNILKFHIKDFHATWGLEKDFNKLKKYFKIYF
HHCCCHHCCCCCCCHHHHHHCCCCCCEEEEEEEEEHHHHHHHCCCHHHHHHHHHHHHHHC
TEKERQSKYFSNLINSKTYEELFENLKVIDMVGDF
CCHHHHHHHHHHHHCCHHHHHHHHCCEEHHHCCCC
>Mature Secondary Structure
MLCSYFMKFLFDIPLPIMILAPMEDVTDTVFRNLIHLIGSAEGEPHIYFTEFISTKGILN
CCHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHCCCCC
GSKQSVQHVFLKPNELNRPLIAQIWGNVPEQFYRAIEILGSMGFWGIDINMGCPKSKIIK
CCHHHHHEEEECCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHH
KGVCSALINNKSLAKEIILASKEACARFNLPLSVKTRHGFSYPEVDDWLGFLLGLEIDML
HHHHHHHHCCHHHHHHHHHHCHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCCHHHH
TVYPRLAVNQSKGPVNFDIFYELVKLRNNLSPSTLIIGNGDVLSLKEARYYVDKYLIDGI
HHCCHHEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCEEEHHHHHHHHHHHHHHHH
MFGRGIFDNLNLFKPFSKHFLDSNLNFRLNILKFHIKDFHATWGLEKDFNKLKKYFKIYF
HHCCCHHCCCCCCCHHHHHHCCCCCCEEEEEEEEEHHHHHHHCCCHHHHHHHHHHHHHHC
TEKERQSKYFSNLINSKTYEELFENLKVIDMVGDF
CCHHHHHHHHHHHHCCHHHHHHHHCCEEHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA