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The map label for this gene is nirB [C]

Identifier: 15595073

GI number: 15595073

Start: 766733

End: 768067

Strand: Reverse

Name: nirB [C]

Synonym: BB0728

Alternate gene names: 15595073

Gene position: 768067-766733 (Counterclockwise)

Preceding gene: 15595074

Following gene: 15595068

Centisome position: 84.34

GC content: 31.61

Gene sequence:

>1335_bases
ATGATGAAAATAATAATTATTGGGGGCACATCAGCAGGAACTAGTGCCGCAGCTAAAGCAAACCGCTTAAACAAAAAGCT
AGACATTACTATCTATGAAAAAACAAATATTGTATCTTTTGGAACCTGTGGCCTGCCTTACTTTGTGGGGGGATTCTTTG
ACAACCCCAATACAATGATCTCAAGAACACAAGAAGAATTCGAAAAAACTGGAATCTCTGTTAAAACTAACCACGAAGTT
ATCAAAGTAGATGCAAAAAACAATACAATTGTAATAAAAAATCAAAAAACAGGAACCATTTTTAACAATACTTACGATCA
ACTTATGATAGCAACTGGTGCAAAACCTATTATTCCACCAATCAATAATATCAATCTAGAAAATTTTCATACTCTGAAAA
ATTTAGAAGACGGTCAAAAAATAAAAAAATTAATGGATAGAGAAGAGATTAAAAATATAGTGATAATTGGTGGTGGATAC
ATTGGAATTGAAATGGTAGAAGCAGCAAAAAATAAAAGAAAAAATGTAAGATTAATTCAACTAGATAAGCACATACTCAT
AGATTCCTTTGACGAAGAAATAGTCACAATAATGGAAGAAGAACTAACAAAAAAGGGGGTTAATCTTCATACAAATGAGT
TTGTAAAAAGTTTAATAGGAGAAAAAAAGGCAGAAGGAGTAGTAACAAACAAAAATACTTATCAAGCTGACGCTGTTATA
CTTGCTACCGGAATAAAACCTGACACTGAATTTTTAGAAAACCAGCTTAAAACTACTAAAAATGGAGCAATAATTGTAAA
TGAGTATGGCGAAACTAGCATAAAAAATATTTTTTCTGCAGGAGATTGTGCAACTATTTATAATATAGTAAGTAAAAAAA
ATGAATACATACCCTTGGCAACAACAGCCAACAAACTTGGAAGAATAGTTGGTGAAAATTTAGCTGGGAATCATACAGCA
TTTAAAGGCACATTGGGCTCAGCTTCAATTAAAATACTATCTTTAGAAGCTGCAAGAACAGGACTTACAGAAAAAGATGC
AAAAAAGCTCCAAATAAAATATAAAACGATTTTTGTAAAGGACAAAAATCATACAAATTATTATCCAGGCCAAGAAGATC
TTTATATTAAATTAATTTATGAGGAAAATACCAAAATAATCCTTGGGGCACAAGCAATAGGAAAAAATGGAGCCGTAATA
AGAATTCATGCTTTATCAATTGCAATCTATTCAAAACTTACAACAAAAGAGCTAGGGATGATGGATTTCTCATATTCCCC
ACCCTTCTCAAGAACTTGGGATATATTAAATATTGCTGGCAATGCTGCCAAATAG

Upstream 100 bases:

>100_bases
GCTCAATGCTTGCAGGCGTTATATCTGCTAAACAGCTCAAACAATTCAACCATAATATATACAACCAAAAAGAGCTTGTA
AACAAATAAATAGGAAAACA

Downstream 100 bases:

>100_bases
AAAGAATTAAATTAATTTAATTCTTCATGCTAATTGGTTGCCCCGTACTTGAAAGAACATCTCTCCAAAAAGAACCATTT
GGATTAACCTTATTTCTGTC

Product: coenzyme A disulfide reductase

Products: NA

Alternate protein names: CoA-disulfide reductase; CoADR [H]

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MMKIIIIGGTSAGTSAAAKANRLNKKLDITIYEKTNIVSFGTCGLPYFVGGFFDNPNTMISRTQEEFEKTGISVKTNHEV
IKVDAKNNTIVIKNQKTGTIFNNTYDQLMIATGAKPIIPPINNINLENFHTLKNLEDGQKIKKLMDREEIKNIVIIGGGY
IGIEMVEAAKNKRKNVRLIQLDKHILIDSFDEEIVTIMEEELTKKGVNLHTNEFVKSLIGEKKAEGVVTNKNTYQADAVI
LATGIKPDTEFLENQLKTTKNGAIIVNEYGETSIKNIFSAGDCATIYNIVSKKNEYIPLATTANKLGRIVGENLAGNHTA
FKGTLGSASIKILSLEAARTGLTEKDAKKLQIKYKTIFVKDKNHTNYYPGQEDLYIKLIYEENTKIILGAQAIGKNGAVI
RIHALSIAIYSKLTTKELGMMDFSYSPPFSRTWDILNIAGNAAK

Sequences:

>Translated_444_residues
MMKIIIIGGTSAGTSAAAKANRLNKKLDITIYEKTNIVSFGTCGLPYFVGGFFDNPNTMISRTQEEFEKTGISVKTNHEV
IKVDAKNNTIVIKNQKTGTIFNNTYDQLMIATGAKPIIPPINNINLENFHTLKNLEDGQKIKKLMDREEIKNIVIIGGGY
IGIEMVEAAKNKRKNVRLIQLDKHILIDSFDEEIVTIMEEELTKKGVNLHTNEFVKSLIGEKKAEGVVTNKNTYQADAVI
LATGIKPDTEFLENQLKTTKNGAIIVNEYGETSIKNIFSAGDCATIYNIVSKKNEYIPLATTANKLGRIVGENLAGNHTA
FKGTLGSASIKILSLEAARTGLTEKDAKKLQIKYKTIFVKDKNHTNYYPGQEDLYIKLIYEENTKIILGAQAIGKNGAVI
RIHALSIAIYSKLTTKELGMMDFSYSPPFSRTWDILNIAGNAAK
>Mature_444_residues
MMKIIIIGGTSAGTSAAAKANRLNKKLDITIYEKTNIVSFGTCGLPYFVGGFFDNPNTMISRTQEEFEKTGISVKTNHEV
IKVDAKNNTIVIKNQKTGTIFNNTYDQLMIATGAKPIIPPINNINLENFHTLKNLEDGQKIKKLMDREEIKNIVIIGGGY
IGIEMVEAAKNKRKNVRLIQLDKHILIDSFDEEIVTIMEEELTKKGVNLHTNEFVKSLIGEKKAEGVVTNKNTYQADAVI
LATGIKPDTEFLENQLKTTKNGAIIVNEYGETSIKNIFSAGDCATIYNIVSKKNEYIPLATTANKLGRIVGENLAGNHTA
FKGTLGSASIKILSLEAARTGLTEKDAKKLQIKYKTIFVKDKNHTNYYPGQEDLYIKLIYEENTKIILGAQAIGKNGAVI
RIHALSIAIYSKLTTKELGMMDFSYSPPFSRTWDILNIAGNAAK

Specific function: Nitrate assimilation (denitrification); second step. [C]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI226437568, Length=265, Percent_Identity=24.9056603773585, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI21389617, Length=265, Percent_Identity=24.9056603773585, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI65787454, Length=265, Percent_Identity=24.9056603773585, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1789065, Length=357, Percent_Identity=25.7703081232493, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1789765, Length=377, Percent_Identity=24.9336870026525, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788892, Length=180, Percent_Identity=28.3333333333333, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1786307, Length=441, Percent_Identity=24.2630385487528, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1789915, Length=308, Percent_Identity=26.6233766233766, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17557007, Length=344, Percent_Identity=25.2906976744186, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24585130, Length=265, Percent_Identity=23.7735849056604, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281359715, Length=187, Percent_Identity=26.2032085561497, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281359713, Length=187, Percent_Identity=26.2032085561497, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24639250, Length=187, Percent_Identity=26.2032085561497, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI18543267, Length=187, Percent_Identity=26.2032085561497, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24639252, Length=187, Percent_Identity=26.2032085561497, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24639257, Length=217, Percent_Identity=25.3456221198157, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017758
- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.14 [H]

Molecular weight: Translated: 49076; Mature: 49076

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKIIIIGGTSAGTSAAAKANRLNKKLDITIYEKTNIVSFGTCGLPYFVGGFFDNPNTMI
CEEEEEEECCCCCCHHHHHHHHCCCEEEEEEEECCCEEEEECCCCCHHHCCEECCCCHHH
SRTQEEFEKTGISVKTNHEVIKVDAKNNTIVIKNQKTGTIFNNTYDQLMIATGAKPIIPP
HHHHHHHHHCCCEEEECCEEEEEECCCCEEEEEECCCCCEECCCCCEEEEECCCCCCCCC
INNINLENFHTLKNLEDGQKIKKLMDREEIKNIVIIGGGYIGIEMVEAAKNKRKNVRLIQ
CCCCCCCHHHHHHCCCCHHHHHHHHCHHHCCEEEEECCCEEEEHHHHHHHCCCCCEEEEE
LDKHILIDSFDEEIVTIMEEELTKKGVNLHTNEFVKSLIGEKKAEGVVTNKNTYQADAVI
ECCEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHCCCEEECCCCEECCEEE
LATGIKPDTEFLENQLKTTKNGAIIVNEYGETSIKNIFSAGDCATIYNIVSKKNEYIPLA
EEECCCCCHHHHHHHHHCCCCCEEEEECCCCHHHHHHHCCCCHHHHHHHHHCCCCEEEEE
TTANKLGRIVGENLAGNHTAFKGTLGSASIKILSLEAARTGLTEKDAKKLQIKYKTIFVK
ECHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEEEEHHHCCCCCCCCCEEEEEEEEEEEE
DKNHTNYYPGQEDLYIKLIYEENTKIILGAQAIGKNGAVIRIHALSIAIYSKLTTKELGM
CCCCCCCCCCCCCEEEEEEEECCCEEEEEEHCCCCCCCEEEEEEEEEEEHHHHHHHHCCC
MDFSYSPPFSRTWDILNIAGNAAK
EECCCCCCCCCCCEEEEECCCCCC
>Mature Secondary Structure
MMKIIIIGGTSAGTSAAAKANRLNKKLDITIYEKTNIVSFGTCGLPYFVGGFFDNPNTMI
CEEEEEEECCCCCCHHHHHHHHCCCEEEEEEEECCCEEEEECCCCCHHHCCEECCCCHHH
SRTQEEFEKTGISVKTNHEVIKVDAKNNTIVIKNQKTGTIFNNTYDQLMIATGAKPIIPP
HHHHHHHHHCCCEEEECCEEEEEECCCCEEEEEECCCCCEECCCCCEEEEECCCCCCCCC
INNINLENFHTLKNLEDGQKIKKLMDREEIKNIVIIGGGYIGIEMVEAAKNKRKNVRLIQ
CCCCCCCHHHHHHCCCCHHHHHHHHCHHHCCEEEEECCCEEEEHHHHHHHCCCCCEEEEE
LDKHILIDSFDEEIVTIMEEELTKKGVNLHTNEFVKSLIGEKKAEGVVTNKNTYQADAVI
ECCEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHCCCEEECCCCEECCEEE
LATGIKPDTEFLENQLKTTKNGAIIVNEYGETSIKNIFSAGDCATIYNIVSKKNEYIPLA
EEECCCCCHHHHHHHHHCCCCCEEEEECCCCHHHHHHHCCCCHHHHHHHHHCCCCEEEEE
TTANKLGRIVGENLAGNHTAFKGTLGSASIKILSLEAARTGLTEKDAKKLQIKYKTIFVK
ECHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEEEEHHHCCCCCCCCCEEEEEEEEEEEE
DKNHTNYYPGQEDLYIKLIYEENTKIILGAQAIGKNGAVIRIHALSIAIYSKLTTKELGM
CCCCCCCCCCCCCEEEEEEEECCCEEEEEEHCCCCCCCEEEEEEEEEEEHHHHHHHHCCC
MDFSYSPPFSRTWDILNIAGNAAK
EECCCCCCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA