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The map label for this gene is pfp [H]

Identifier: 15595072

GI number: 15595072

Start: 765370

End: 766716

Strand: Direct

Name: pfp [H]

Synonym: BB0727

Alternate gene names: 15595072

Gene position: 765370-766716 (Clockwise)

Preceding gene: 15595071

Following gene: 15595078

Centisome position: 84.04

GC content: 34.0

Gene sequence:

>1347_bases
TTGGTGTATAGAATTAAAAATGAAAATTTAGATTTTAAAATAGATAGCTTGGGAGAATGCAAGCAAAACAATCCTTTGAT
TGATTTTTATGCTAGTGAGGGTTCTTCCCATTTTGTTAATGAAAAAAATAAAATCAAGTTTAGTGTATATAGGAATGAGG
ATAAGGGAGATAGGTATGAAGATGTTCTTTTAGAAAAAGCTGGGCCTAGAGAAAAAATTTATTTTGTGCCCAGGCATGTT
AAAGCTGCTATTACTACTTGTGGTGGGCTTTGTCCTGGTTTTAACGATGTTATTCGCTCTATTGTGCGAACTTTATGGAA
AATATATGGGGTTCGCAATATTTATGGAGTAAAATTTGGATATCAAGGGCTTCTTCCTGAGTCAAATTCACCTTTTATTA
ATCTTAATCCAGATGTTGTTGATGATATTAATAAATTTGGAGGTACTATTCTTGGTTCTTCAAGGGGTGGTATTAAGCCT
GTGGAAATAGTTGATACTTTAGAGAGAATGAACATTAATATGATTTTTAACATTGGCGGGGATGGTACTCAAAAGGGGTC
TCTTCTTATTGCTGAGGAGATAGAAAAAAGAAATTTAAAAATAGCAGTTGTGGGCATTCCTAAAACCGTAGACAATGATT
TTATGTTTGTTCAAAAATCTTTTGGATTTGAGACTGCTGTAGAACAAGCTGTTGCAGCTGTTGCTGGTGCTCATTTTGAA
GCTAATAGTGCTTATAATGGCATTGGACTTGTTAAAGTTATGGGACGGGATTCTGGCTTTATTGCTGCTCATACCGCACT
TTCTTCTAATGATGTTAATTTTTGTTTAATCCCAGAACTTGATTTTGACATAGAAGGTCCTAATGGATTTCTTGTTCATC
TTGAAAGACGACTTTTAGAGAAAGAAAGCTTAGAAGAGATTCCCCATGCAGTAATATTGATAGCAGAAGGAGCAGGCCAA
AAATATTTTGATCATTTTCCTAAGAAGAAAGACGATTCTGGGAATTTGCTTTATGAGGATATTGGGCTTTATATTAAAGA
TAAAATTACAGAATATTTTAAAGCAAAAAATATACAATTTACTCTTAAATACATTGATCCCAGCTATATTATTAGAAGTT
CACCTGCTAATGCCAGTGATTCGCTTTATTGTGCTAGGCTTGGGTCGAATGCTGTGCATGCTGCAATGGCTGGTAAGACA
AAAATGTTGATTAGTTTATGGAGTACAAAATTTGTTCATATACCTATTAAGATGGCAGTAATTGACAGAAATAAGGTTAA
TCCAAATGGTTCTTTTTGGAGAGATGTTCTTTCAAGTACGGGGCAACCAATTAGCATGAAGAATTAA

Upstream 100 bases:

>100_bases
TGAAGGGTCTCCTTATATTTACGATGACATATACGATATGATTAAAGATCAAAGTCGATTTATTAGAAAATAATTAGGTT
ATAATTCTATAAAGGAGTAT

Downstream 100 bases:

>100_bases
ATTAATTTAATTCTTTCTATTTGGCAGCATTGCCAGCAATATTTAATATATCCCAAGTTCTTGAGAAGGGTGGGGAATAT
GAGAAATCCATCATCCCTAG

Product: diphosphate--fructose-6-phosphate 1-phosphotransferase

Products: NA

Alternate protein names: 6-phosphofructokinase, pyrophosphate dependent; PPi-dependent phosphofructokinase; PPi-PFK; Pyrophosphate-dependent 6-phosphofructose-1-kinase [H]

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MVYRIKNENLDFKIDSLGECKQNNPLIDFYASEGSSHFVNEKNKIKFSVYRNEDKGDRYEDVLLEKAGPREKIYFVPRHV
KAAITTCGGLCPGFNDVIRSIVRTLWKIYGVRNIYGVKFGYQGLLPESNSPFINLNPDVVDDINKFGGTILGSSRGGIKP
VEIVDTLERMNINMIFNIGGDGTQKGSLLIAEEIEKRNLKIAVVGIPKTVDNDFMFVQKSFGFETAVEQAVAAVAGAHFE
ANSAYNGIGLVKVMGRDSGFIAAHTALSSNDVNFCLIPELDFDIEGPNGFLVHLERRLLEKESLEEIPHAVILIAEGAGQ
KYFDHFPKKKDDSGNLLYEDIGLYIKDKITEYFKAKNIQFTLKYIDPSYIIRSSPANASDSLYCARLGSNAVHAAMAGKT
KMLISLWSTKFVHIPIKMAVIDRNKVNPNGSFWRDVLSSTGQPISMKN

Sequences:

>Translated_448_residues
MVYRIKNENLDFKIDSLGECKQNNPLIDFYASEGSSHFVNEKNKIKFSVYRNEDKGDRYEDVLLEKAGPREKIYFVPRHV
KAAITTCGGLCPGFNDVIRSIVRTLWKIYGVRNIYGVKFGYQGLLPESNSPFINLNPDVVDDINKFGGTILGSSRGGIKP
VEIVDTLERMNINMIFNIGGDGTQKGSLLIAEEIEKRNLKIAVVGIPKTVDNDFMFVQKSFGFETAVEQAVAAVAGAHFE
ANSAYNGIGLVKVMGRDSGFIAAHTALSSNDVNFCLIPELDFDIEGPNGFLVHLERRLLEKESLEEIPHAVILIAEGAGQ
KYFDHFPKKKDDSGNLLYEDIGLYIKDKITEYFKAKNIQFTLKYIDPSYIIRSSPANASDSLYCARLGSNAVHAAMAGKT
KMLISLWSTKFVHIPIKMAVIDRNKVNPNGSFWRDVLSSTGQPISMKN
>Mature_448_residues
MVYRIKNENLDFKIDSLGECKQNNPLIDFYASEGSSHFVNEKNKIKFSVYRNEDKGDRYEDVLLEKAGPREKIYFVPRHV
KAAITTCGGLCPGFNDVIRSIVRTLWKIYGVRNIYGVKFGYQGLLPESNSPFINLNPDVVDDINKFGGTILGSSRGGIKP
VEIVDTLERMNINMIFNIGGDGTQKGSLLIAEEIEKRNLKIAVVGIPKTVDNDFMFVQKSFGFETAVEQAVAAVAGAHFE
ANSAYNGIGLVKVMGRDSGFIAAHTALSSNDVNFCLIPELDFDIEGPNGFLVHLERRLLEKESLEEIPHAVILIAEGAGQ
KYFDHFPKKKDDSGNLLYEDIGLYIKDKITEYFKAKNIQFTLKYIDPSYIIRSSPANASDSLYCARLGSNAVHAAMAGKT
KMLISLWSTKFVHIPIKMAVIDRNKVNPNGSFWRDVLSSTGQPISMKN

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI266453619, Length=366, Percent_Identity=24.8633879781421, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI266453768, Length=366, Percent_Identity=24.8633879781421, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI266453748, Length=366, Percent_Identity=24.8633879781421, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI4505749, Length=366, Percent_Identity=24.8633879781421, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI11321601, Length=260, Percent_Identity=27.3076923076923, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI48762920, Length=365, Percent_Identity=24.3835616438356, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI1790350, Length=361, Percent_Identity=25.207756232687, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI25147584, Length=372, Percent_Identity=25.5376344086022, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17558788, Length=348, Percent_Identity=23.2758620689655, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17647809, Length=373, Percent_Identity=24.6648793565684, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24652337, Length=373, Percent_Identity=24.6648793565684, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI28573326, Length=372, Percent_Identity=24.7311827956989, Blast_Score=82, Evalue=5e-16,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.90 [H]

Molecular weight: Translated: 49888; Mature: 49888

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVYRIKNENLDFKIDSLGECKQNNPLIDFYASEGSSHFVNEKNKIKFSVYRNEDKGDRYE
CEEEEECCCCCEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEEEEECCCCCCHHH
DVLLEKAGPREKIYFVPRHVKAAITTCGGLCPGFNDVIRSIVRTLWKIYGVRNIYGVKFG
HHHHHCCCCCCEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEC
YQGLLPESNSPFINLNPDVVDDINKFGGTILGSSRGGIKPVEIVDTLERMNINMIFNIGG
CCCCCCCCCCCEEEECCHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHCCEEEEEEECC
DGTQKGSLLIAEEIEKRNLKIAVVGIPKTVDNDFMFVQKSFGFETAVEQAVAAVAGAHFE
CCCCCCCEEEEEHHHHCCEEEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCEE
ANSAYNGIGLVKVMGRDSGFIAAHTALSSNDVNFCLIPELDFDIEGPNGFLVHLERRLLE
CCCCCCCEEEEEEECCCCCEEEEEEEECCCCCCEEEECCCCCEEECCCCEEEEEHHHHHH
KESLEEIPHAVILIAEGAGQKYFDHFPKKKDDSGNLLYEDIGLYIKDKITEYFKAKNIQF
HHHHHHCCCEEEEEECCCCCHHHHHCCCCCCCCCCEEEEHHCCHHHHHHHHHHHCCCEEE
TLKYIDPSYIIRSSPANASDSLYCARLGSNAVHAAMAGKTKMLISLWSTKFVHIPIKMAV
EEEEECCCEEEECCCCCCCCCEEEEECCCCCEEHHHCCCCEEEEEEECCEEEEEEEEEEE
IDRNKVNPNGSFWRDVLSSTGQPISMKN
EECCCCCCCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MVYRIKNENLDFKIDSLGECKQNNPLIDFYASEGSSHFVNEKNKIKFSVYRNEDKGDRYE
CEEEEECCCCCEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEEEEECCCCCCHHH
DVLLEKAGPREKIYFVPRHVKAAITTCGGLCPGFNDVIRSIVRTLWKIYGVRNIYGVKFG
HHHHHCCCCCCEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEC
YQGLLPESNSPFINLNPDVVDDINKFGGTILGSSRGGIKPVEIVDTLERMNINMIFNIGG
CCCCCCCCCCCEEEECCHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHCCEEEEEEECC
DGTQKGSLLIAEEIEKRNLKIAVVGIPKTVDNDFMFVQKSFGFETAVEQAVAAVAGAHFE
CCCCCCCEEEEEHHHHCCEEEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCEE
ANSAYNGIGLVKVMGRDSGFIAAHTALSSNDVNFCLIPELDFDIEGPNGFLVHLERRLLE
CCCCCCCEEEEEEECCCCCEEEEEEEECCCCCCEEEECCCCCEEECCCCEEEEEHHHHHH
KESLEEIPHAVILIAEGAGQKYFDHFPKKKDDSGNLLYEDIGLYIKDKITEYFKAKNIQF
HHHHHHCCCEEEEEECCCCCHHHHHCCCCCCCCCCEEEEHHCCHHHHHHHHHHHCCCEEE
TLKYIDPSYIIRSSPANASDSLYCARLGSNAVHAAMAGKTKMLISLWSTKFVHIPIKMAV
EEEEECCCEEEECCCCCCCCCEEEEECCCCCEEHHHCCCCEEEEEEECCEEEEEEEEEEE
IDRNKVNPNGSFWRDVLSSTGQPISMKN
EECCCCCCCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8550409 [H]