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The map label for this gene is thrS [H]

Identifier: 15595065

GI number: 15595065

Start: 757836

End: 759581

Strand: Direct

Name: thrS [H]

Synonym: BB0720

Alternate gene names: 15595065

Gene position: 757836-759581 (Clockwise)

Preceding gene: 15595064

Following gene: 15595066

Centisome position: 83.21

GC content: 31.86

Gene sequence:

>1746_bases
GTGAGCAAAGATTTAGATAAAGAAGATATTCTTTACAAAAAAAGACACTCAATAGCTCATGTTATGGCAGAAGCTGTTCT
TGATTTATTTCCAAATACCAAGATTGCAATAGGTCCTCCTATTAAAGATGGTTTTTATTATGATTTTGAATTTAAAAAGC
AGATTACAGAAGATTCTCTTTTAGACATAGAAAATAGAATGAGAGAAATTTTAAAGACCGGAAGTTCTTTTGAAAAAGAG
ATAATAAGCGTAGAACAGGCTCTTGAAATTTTTAAAGATGAACCTTATAAGATTGATTTGATTAAAAATTTTGATTTACA
AAATGAAGTTTCTATTTACAAGAGTCACAATTTTGTTGATCTTTGTAGAGGTCCTCATGTTGAGAATATGAATAAAATTG
ATCCAAAGGCATTTAAGCTTACTAGTATTGCTGGGGCTTATTGGCGGGGCAGTGAAAAAAACCCAATGCTTACCAGAATT
TATGGAACTTTATGGAATAATGAAAAAGAACTGAGATCTTATCTTAATTTGAGAGAGGAAATAAAAAAAAGAGATCATAG
AAAGCTTGGAAAAGAGCTTGATTTATTTTCTATACATGAAGAGATTGGACCAGGACTTGTTTTTTTTCATCCCAATGGTG
CCAAAATAAGAGCTTTAATAGAAGATTTTTGGAGAGAAGAGCACTCCAAAAATGGGTATGATATTCTTTTTACTCCTCAT
ATTGGCAAATCTTGGCTTTGGCAAACTTCTGGTCATTTAGACTTTTATAAGGATAGCATGTTTGAAAAAATAGAAATGGA
TAAAAGTGATTATTATCTTAAACCCATGAATTGTCCTTTTCATATTGCMATTTACAATACAGGTAAGCATTCTTATAGAG
ATTTGCCATTTAGATGGGCCGAACTTGGCACTGTGTATCGTTATGAAAAGATAGGTGCTTTGCATGGTATGATGAGAGCC
AGAGGGTTTACTCAGGATGATGCTCATATTATATGCACCCATTCTCAGGTTTTAGATGAGATTAAAGAAGTTCTTAGGTT
TGCTATTTATATGTGGAGTAAATTTGGCTTTAGCAACCCAAAGGCATATCTTTCTACAAAGCCTGACAAATCTGTTGGCA
ATGATTCTGATTGGGAAATGTCTTTAAAAGTTCTTGAAGAGACTTTAAGCGATTTTGAAGTTCCTTATGAAATTGATAAG
GGTGGAGGTGCTTTTTATGGGCCTAAAATTGATCTTAAGATAGTTGATTCGCTTGAGAGAGAGTGGCAGATGAGTACAAT
TCAATTTGATTTTAATCTCCCTGAGAGATTTAATATGACTTATACCGCTGAGGATGGTAAAGAAAAAAGACCATTTATGA
TTCATAGAGCTTTGTTGGGATCTATTGAAAGATTTTTTGGAATTCTTGTAGAGCACTATGGTGGTGCTTTTCCTTTATGG
TTATCTCCTGTTCAAGTAGTAATCATTCCTGTTAACAATATTGTCGAAGATTATGCTATTAAGGTTTTTAATAAATTTAA
AAATGAGGGGATTAGAATAAAGCTTGATAATAGTTCCTCAAGAATGAATGCTAAAATTAGAGAATATCAGGCTAAAAAAA
TACCTTATATGTTTATAATTGGTGAGAGAGAAGCAACAGAAGAGAGAATATCTATTAGAACAAGAACAAATGAGCAAATA
AATGGAATGAAACTTGATGAAGCTCTTAAATTTATTTTATTTAAAATAAGAGATAAGGAGATTTGA

Upstream 100 bases:

>100_bases
ATTTAGTAATTATAAATATTTGAGGTGCGTATTTTTACGTAAAAGTGATGAGTGTGAATTTTAATTTTTTAATAAAAAGC
GAAAGGCAAAAGGTATTGTT

Downstream 100 bases:

>100_bases
TAAATTGAATAATTTAATCAAGGTCATTACCCCTAATAAAATAACATTAGTTAGAATTGCACTTTCCTTTATCATATTAA
TTTTATTTTTTTTGGAAAAT

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 581; Mature: 580

Protein sequence:

>581_residues
MSKDLDKEDILYKKRHSIAHVMAEAVLDLFPNTKIAIGPPIKDGFYYDFEFKKQITEDSLLDIENRMREILKTGSSFEKE
IISVEQALEIFKDEPYKIDLIKNFDLQNEVSIYKSHNFVDLCRGPHVENMNKIDPKAFKLTSIAGAYWRGSEKNPMLTRI
YGTLWNNEKELRSYLNLREEIKKRDHRKLGKELDLFSIHEEIGPGLVFFHPNGAKIRALIEDFWREEHSKNGYDILFTPH
IGKSWLWQTSGHLDFYKDSMFEKIEMDKSDYYLKPMNCPFHIAIYNTGKHSYRDLPFRWAELGTVYRYEKIGALHGMMRA
RGFTQDDAHIICTHSQVLDEIKEVLRFAIYMWSKFGFSNPKAYLSTKPDKSVGNDSDWEMSLKVLEETLSDFEVPYEIDK
GGGAFYGPKIDLKIVDSLEREWQMSTIQFDFNLPERFNMTYTAEDGKEKRPFMIHRALLGSIERFFGILVEHYGGAFPLW
LSPVQVVIIPVNNIVEDYAIKVFNKFKNEGIRIKLDNSSSRMNAKIREYQAKKIPYMFIIGEREATEERISIRTRTNEQI
NGMKLDEALKFILFKIRDKEI

Sequences:

>Translated_581_residues
MSKDLDKEDILYKKRHSIAHVMAEAVLDLFPNTKIAIGPPIKDGFYYDFEFKKQITEDSLLDIENRMREILKTGSSFEKE
IISVEQALEIFKDEPYKIDLIKNFDLQNEVSIYKSHNFVDLCRGPHVENMNKIDPKAFKLTSIAGAYWRGSEKNPMLTRI
YGTLWNNEKELRSYLNLREEIKKRDHRKLGKELDLFSIHEEIGPGLVFFHPNGAKIRALIEDFWREEHSKNGYDILFTPH
IGKSWLWQTSGHLDFYKDSMFEKIEMDKSDYYLKPMNCPFHIAIYNTGKHSYRDLPFRWAELGTVYRYEKIGALHGMMRA
RGFTQDDAHIICTHSQVLDEIKEVLRFAIYMWSKFGFSNPKAYLSTKPDKSVGNDSDWEMSLKVLEETLSDFEVPYEIDK
GGGAFYGPKIDLKIVDSLEREWQMSTIQFDFNLPERFNMTYTAEDGKEKRPFMIHRALLGSIERFFGILVEHYGGAFPLW
LSPVQVVIIPVNNIVEDYAIKVFNKFKNEGIRIKLDNSSSRMNAKIREYQAKKIPYMFIIGEREATEERISIRTRTNEQI
NGMKLDEALKFILFKIRDKEI
>Mature_580_residues
SKDLDKEDILYKKRHSIAHVMAEAVLDLFPNTKIAIGPPIKDGFYYDFEFKKQITEDSLLDIENRMREILKTGSSFEKEI
ISVEQALEIFKDEPYKIDLIKNFDLQNEVSIYKSHNFVDLCRGPHVENMNKIDPKAFKLTSIAGAYWRGSEKNPMLTRIY
GTLWNNEKELRSYLNLREEIKKRDHRKLGKELDLFSIHEEIGPGLVFFHPNGAKIRALIEDFWREEHSKNGYDILFTPHI
GKSWLWQTSGHLDFYKDSMFEKIEMDKSDYYLKPMNCPFHIAIYNTGKHSYRDLPFRWAELGTVYRYEKIGALHGMMRAR
GFTQDDAHIICTHSQVLDEIKEVLRFAIYMWSKFGFSNPKAYLSTKPDKSVGNDSDWEMSLKVLEETLSDFEVPYEIDKG
GGAFYGPKIDLKIVDSLEREWQMSTIQFDFNLPERFNMTYTAEDGKEKRPFMIHRALLGSIERFFGILVEHYGGAFPLWL
SPVQVVIIPVNNIVEDYAIKVFNKFKNEGIRIKLDNSSSRMNAKIREYQAKKIPYMFIIGEREATEERISIRTRTNEQIN
GMKLDEALKFILFKIRDKEI

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38570111, Length=550, Percent_Identity=41.0909090909091, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI38202255, Length=546, Percent_Identity=40.2930402930403, Blast_Score=420, Evalue=1e-117,
Organism=Homo sapiens, GI20070344, Length=577, Percent_Identity=35.3552859618718, Blast_Score=373, Evalue=1e-103,
Organism=Escherichia coli, GI1788013, Length=570, Percent_Identity=42.6315789473684, Blast_Score=502, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI71984184, Length=557, Percent_Identity=39.6768402154399, Blast_Score=418, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI71984192, Length=557, Percent_Identity=39.6768402154399, Blast_Score=418, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6322112, Length=578, Percent_Identity=37.3702422145329, Blast_Score=406, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6322654, Length=400, Percent_Identity=38.25, Blast_Score=263, Evalue=5e-71,
Organism=Drosophila melanogaster, GI24583839, Length=568, Percent_Identity=39.612676056338, Blast_Score=438, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24583842, Length=568, Percent_Identity=39.612676056338, Blast_Score=437, Evalue=1e-123,
Organism=Drosophila melanogaster, GI28574141, Length=568, Percent_Identity=39.612676056338, Blast_Score=437, Evalue=1e-123,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 68173; Mature: 68042

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKDLDKEDILYKKRHSIAHVMAEAVLDLFPNTKIAIGPPIKDGFYYDFEFKKQITEDSL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEHHHHHCCCHHH
LDIENRMREILKTGSSFEKEIISVEQALEIFKDEPYKIDLIKNFDLQNEVSIYKSHNFVD
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEECCCCHH
LCRGPHVENMNKIDPKAFKLTSIAGAYWRGSEKNPMLTRIYGTLWNNEKELRSYLNLREE
HHCCCCCCCCCCCCCCCEEEHHHHHHHCCCCCCCCCCEEEHHHHHCCHHHHHHHHHHHHH
IKKRDHRKLGKELDLFSIHEEIGPGLVFFHPNGAKIRALIEDFWREEHSKNGYDILFTPH
HHHHHHHHHCCCCCHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEECC
IGKSWLWQTSGHLDFYKDSMFEKIEMDKSDYYLKPMNCPFHIAIYNTGKHSYRDLPFRWA
CCCCCEEECCCCCCHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCHHH
ELGTVYRYEKIGALHGMMRARGFTQDDAHIICTHSQVLDEIKEVLRFAIYMWSKFGFSNP
HHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCC
KAYLSTKPDKSVGNDSDWEMSLKVLEETLSDFEVPYEIDKGGGAFYGPKIDLKIVDSLER
CEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCEEECCEEEEHHHHHHHH
EWQMSTIQFDFNLPERFNMTYTAEDGKEKRPFMIHRALLGSIERFFGILVEHYGGAFPLW
HHEEEEEEEECCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
LSPVQVVIIPVNNIVEDYAIKVFNKFKNEGIRIKLDNSSSRMNAKIREYQAKKIPYMFII
ECCEEEEEECHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEE
GEREATEERISIRTRTNEQINGMKLDEALKFILFKIRDKEI
CCCCCCCHHEEEEECCCCCCCCEEHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SKDLDKEDILYKKRHSIAHVMAEAVLDLFPNTKIAIGPPIKDGFYYDFEFKKQITEDSL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEHHHHHCCCHHH
LDIENRMREILKTGSSFEKEIISVEQALEIFKDEPYKIDLIKNFDLQNEVSIYKSHNFVD
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEECCCCHH
LCRGPHVENMNKIDPKAFKLTSIAGAYWRGSEKNPMLTRIYGTLWNNEKELRSYLNLREE
HHCCCCCCCCCCCCCCCEEEHHHHHHHCCCCCCCCCCEEEHHHHHCCHHHHHHHHHHHHH
IKKRDHRKLGKELDLFSIHEEIGPGLVFFHPNGAKIRALIEDFWREEHSKNGYDILFTPH
HHHHHHHHHCCCCCHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEECC
IGKSWLWQTSGHLDFYKDSMFEKIEMDKSDYYLKPMNCPFHIAIYNTGKHSYRDLPFRWA
CCCCCEEECCCCCCHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCHHH
ELGTVYRYEKIGALHGMMRARGFTQDDAHIICTHSQVLDEIKEVLRFAIYMWSKFGFSNP
HHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCC
KAYLSTKPDKSVGNDSDWEMSLKVLEETLSDFEVPYEIDKGGGAFYGPKIDLKIVDSLER
CEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCEEECCEEEEHHHHHHHH
EWQMSTIQFDFNLPERFNMTYTAEDGKEKRPFMIHRALLGSIERFFGILVEHYGGAFPLW
HHEEEEEEEECCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
LSPVQVVIIPVNNIVEDYAIKVFNKFKNEGIRIKLDNSSSRMNAKIREYQAKKIPYMFII
ECCEEEEEECHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEE
GEREATEERISIRTRTNEQINGMKLDEALKFILFKIRDKEI
CCCCCCCHHEEEEECCCCCCCCEEHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA