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The map label for this gene is mrdA [H]

Identifier: 15595063

GI number: 15595063

Start: 754550

End: 756349

Strand: Direct

Name: mrdA [H]

Synonym: BB0718

Alternate gene names: 15595063

Gene position: 754550-756349 (Clockwise)

Preceding gene: 15595062

Following gene: 15595064

Centisome position: 82.85

GC content: 31.56

Gene sequence:

>1800_bases
GTGGGTGTTATAACAAATTTTAGATACAAGTTCGGCATATTTTTTTTAATAGTAATTATGGTGCTTTATTTGGCGATTTT
ATTCCAAATGCAAATTGGTAAGCATTTGTTTTATGACAGAGAAGCCAATGTTTTTTTATCAAGATTGGAAAAAATCAATG
CCTCAAGAGGTGAAATTTTGGATTCTAATTCCAATGTTTTGGCAAATAATTTAACTATGTTTATCTTAAAGATAAGCTTA
CAACAGTATTACAATATGCCTGCTGCTACTAGAATTGAGATGATAGATTTTTTATCAAGCACTCTAGATATTGATAAATC
AATTATTTTGTCTAAACTTCAAGAGCCTGGTGGATATCTTAAAGATGTTGAAATAATTGAACTTACCCCAAAGATGCTTT
TCAAAATTTCTGAAAAAAAGTTTTATTATCCTGCTCTTTTGTGGACCTATTCTTTTAAGCGTAACTATTTAGTTGACGAT
TCATATTCTCATTCAATCGGTTATGTTGGGCAAATAAATCAAAGAGAACTTAGGACGTTTTACAATGTTAGTGGGTATGA
TAATACTTCTACGATTGGAAAGTTGGGCGTTGAACAAGTTTATGATAATTACATTAGAGGGCAAGAGGGATTAATAAAAT
ACAAGGTAGATTCTAAGGAGAGAAGAATAGACGATGGTTCTATTATAAAAAATATGGTGCCCGGTAATGATGTTGTGCTT
AATATCAATAAAGATATTCAAGATCTTGCTAAGAATGCTTTAGGCAAAAGGTATGGTTCTGTTGTAGTATTAAAACCATC
AACAGGTGCTGTTCTTGCTCTTCACAATTATCCTTATTATTCTATGAAAGATGTTTACAATAAAGATAATAAGGAAGATT
ACTCTTTTTTAAATAAAGCAATTCAATCTGTTTACCCGCCTGCGTCTATTTTTAAATTAGTTGTTGCTGCTGCCATTCTT
GAAGAAAGAGTTATAGACAAAGATCGTAAAATTTATTGTCCTGGATATTTTAAAGTTGGAAATAGAATTTTTCATTGCTG
GAAGCCCGGAGGTCATGGGTATGTTAATTTAGAAGAGGCGATTGCACATTCTTCTAATGTTTATTTTTATACACTTGGGC
TTAAGTATCTTGGAGTTGATAGAATTAGAAAATATGCAAAAGAATTTGGGTTTGGAGAAAAAACAGGAATTGATTTGCCA
AATGAAGTAGCTGGCCTTCTTCCTAGTCCTGAGTGGAAAGAAAAAACTTTTAATCAGCCTTGGGTAGGAGGAGATACTGT
AAATTTTTCAATAGGTCAAGGATTTTTAAATGCTACTCCTATGCAGATTGTTAATATGGTTGCTATGATTGCAAATGAAG
GTGTTGTATATAAACCTAGAATTGTAAATAAAATTTTAAAAGGTGGTACTAATAAGGTTGTTCTTGAAAATAAACCAGAA
ATATTAAGAAAGACAAATCTTATTAGTAAAAACACATTTAAACTTCTGAAAAAATATATGAGAAGTGTTATAACTTATGG
TACAGCAAGATATGCAGTTCTTACCAAAGCTGTTGAGGTTGGAGGAAAAACAGGTACTGGTCAAACTGGAATAGATGGTT
TTGAAAATAGTTCTTTTATTGGACTTGCCCCTTATAACGGTTCAGCTGATAATCAAATTATTGTTTTTAGTTTGGTTGAG
GCAAAAAGTAATGTGGATTGGTGGCCTGCAAAATCTACAGATTTAATAATGCAAGGCATTTTTGCAAATCAAAGTTATGA
AGATATTCTTAAAGGTTATAGGCCGTGGTATATTAGGTAG

Upstream 100 bases:

>100_bases
TCAACCTTGATCTTTTTGTAAATATAATGTCTATTAACTTTTTATATCCAATTCAAAATTATTTTACTAGAAATTTTTAT
TCTTTTAAAGAGGATTATTA

Downstream 100 bases:

>100_bases
ATTAATGGTTTTTAGAAAAAATTATGATTATTTGGCTTTGATAAGCTTACTTATAGTTTCTTTTGTTGGTATATTGTTGA
TTTATTCTAGCGATTATAAT

Product: penicillin-binding protein

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 599; Mature: 598

Protein sequence:

>599_residues
MGVITNFRYKFGIFFLIVIMVLYLAILFQMQIGKHLFYDREANVFLSRLEKINASRGEILDSNSNVLANNLTMFILKISL
QQYYNMPAATRIEMIDFLSSTLDIDKSIILSKLQEPGGYLKDVEIIELTPKMLFKISEKKFYYPALLWTYSFKRNYLVDD
SYSHSIGYVGQINQRELRTFYNVSGYDNTSTIGKLGVEQVYDNYIRGQEGLIKYKVDSKERRIDDGSIIKNMVPGNDVVL
NINKDIQDLAKNALGKRYGSVVVLKPSTGAVLALHNYPYYSMKDVYNKDNKEDYSFLNKAIQSVYPPASIFKLVVAAAIL
EERVIDKDRKIYCPGYFKVGNRIFHCWKPGGHGYVNLEEAIAHSSNVYFYTLGLKYLGVDRIRKYAKEFGFGEKTGIDLP
NEVAGLLPSPEWKEKTFNQPWVGGDTVNFSIGQGFLNATPMQIVNMVAMIANEGVVYKPRIVNKILKGGTNKVVLENKPE
ILRKTNLISKNTFKLLKKYMRSVITYGTARYAVLTKAVEVGGKTGTGQTGIDGFENSSFIGLAPYNGSADNQIIVFSLVE
AKSNVDWWPAKSTDLIMQGIFANQSYEDILKGYRPWYIR

Sequences:

>Translated_599_residues
MGVITNFRYKFGIFFLIVIMVLYLAILFQMQIGKHLFYDREANVFLSRLEKINASRGEILDSNSNVLANNLTMFILKISL
QQYYNMPAATRIEMIDFLSSTLDIDKSIILSKLQEPGGYLKDVEIIELTPKMLFKISEKKFYYPALLWTYSFKRNYLVDD
SYSHSIGYVGQINQRELRTFYNVSGYDNTSTIGKLGVEQVYDNYIRGQEGLIKYKVDSKERRIDDGSIIKNMVPGNDVVL
NINKDIQDLAKNALGKRYGSVVVLKPSTGAVLALHNYPYYSMKDVYNKDNKEDYSFLNKAIQSVYPPASIFKLVVAAAIL
EERVIDKDRKIYCPGYFKVGNRIFHCWKPGGHGYVNLEEAIAHSSNVYFYTLGLKYLGVDRIRKYAKEFGFGEKTGIDLP
NEVAGLLPSPEWKEKTFNQPWVGGDTVNFSIGQGFLNATPMQIVNMVAMIANEGVVYKPRIVNKILKGGTNKVVLENKPE
ILRKTNLISKNTFKLLKKYMRSVITYGTARYAVLTKAVEVGGKTGTGQTGIDGFENSSFIGLAPYNGSADNQIIVFSLVE
AKSNVDWWPAKSTDLIMQGIFANQSYEDILKGYRPWYIR
>Mature_598_residues
GVITNFRYKFGIFFLIVIMVLYLAILFQMQIGKHLFYDREANVFLSRLEKINASRGEILDSNSNVLANNLTMFILKISLQ
QYYNMPAATRIEMIDFLSSTLDIDKSIILSKLQEPGGYLKDVEIIELTPKMLFKISEKKFYYPALLWTYSFKRNYLVDDS
YSHSIGYVGQINQRELRTFYNVSGYDNTSTIGKLGVEQVYDNYIRGQEGLIKYKVDSKERRIDDGSIIKNMVPGNDVVLN
INKDIQDLAKNALGKRYGSVVVLKPSTGAVLALHNYPYYSMKDVYNKDNKEDYSFLNKAIQSVYPPASIFKLVVAAAILE
ERVIDKDRKIYCPGYFKVGNRIFHCWKPGGHGYVNLEEAIAHSSNVYFYTLGLKYLGVDRIRKYAKEFGFGEKTGIDLPN
EVAGLLPSPEWKEKTFNQPWVGGDTVNFSIGQGFLNATPMQIVNMVAMIANEGVVYKPRIVNKILKGGTNKVVLENKPEI
LRKTNLISKNTFKLLKKYMRSVITYGTARYAVLTKAVEVGGKTGTGQTGIDGFENSSFIGLAPYNGSADNQIIVFSLVEA
KSNVDWWPAKSTDLIMQGIFANQSYEDILKGYRPWYIR

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=540, Percent_Identity=29.0740740740741, Blast_Score=224, Evalue=2e-59,
Organism=Escherichia coli, GI1786272, Length=555, Percent_Identity=22.5225225225225, Blast_Score=90, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 67998; Mature: 67867

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVITNFRYKFGIFFLIVIMVLYLAILFQMQIGKHLFYDREANVFLSRLEKINASRGEIL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEE
DSNSNVLANNLTMFILKISLQQYYNMPAATRIEMIDFLSSTLDIDKSIILSKLQEPGGYL
CCCCCEECCCCEEEHHHEEHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCC
KDVEIIELTPKMLFKISEKKFYYPALLWTYSFKRNYLVDDSYSHSIGYVGQINQRELRTF
CCEEEEEECHHHHHEECCCCEECCEEEEEEECCCCEEECCCCCCCCCCCCCCCHHHHHHH
YNVSGYDNTSTIGKLGVEQVYDNYIRGQEGLIKYKVDSKERRIDDGSIIKNMVPGNDVVL
HCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHCCCCCHHHHHCCCCCCEEE
NINKDIQDLAKNALGKRYGSVVVLKPSTGAVLALHNYPYYSMKDVYNKDNKEDYSFLNKA
EECCCHHHHHHHHHHHHHCCEEEEECCCCCEEEEECCCCCHHHHHHCCCCCHHHHHHHHH
IQSVYPPASIFKLVVAAAILEERVIDKDRKIYCPGYFKVGNRIFHCWKPGGHGYVNLEEA
HHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHCCEEEEEECCCCCCEEEHHHH
IAHSSNVYFYTLGLKYLGVDRIRKYAKEFGFGEKTGIDLPNEVAGLLPSPEWKEKTFNQP
HHCCCCEEEEEECHHHHCHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCHHHHCCCCC
WVGGDTVNFSIGQGFLNATPMQIVNMVAMIANEGVVYKPRIVNKILKGGTNKVVLENKPE
CCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEECHHHHHHHHHCCCCEEEECCCHH
ILRKTNLISKNTFKLLKKYMRSVITYGTARYAVLTKAVEVGGKTGTGQTGIDGFENSSFI
HHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEE
GLAPYNGSADNQIIVFSLVEAKSNVDWWPAKSTDLIMQGIFANQSYEDILKGYRPWYIR
EEECCCCCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCHHHHHCCCCCCCCC
>Mature Secondary Structure 
GVITNFRYKFGIFFLIVIMVLYLAILFQMQIGKHLFYDREANVFLSRLEKINASRGEIL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEE
DSNSNVLANNLTMFILKISLQQYYNMPAATRIEMIDFLSSTLDIDKSIILSKLQEPGGYL
CCCCCEECCCCEEEHHHEEHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCC
KDVEIIELTPKMLFKISEKKFYYPALLWTYSFKRNYLVDDSYSHSIGYVGQINQRELRTF
CCEEEEEECHHHHHEECCCCEECCEEEEEEECCCCEEECCCCCCCCCCCCCCCHHHHHHH
YNVSGYDNTSTIGKLGVEQVYDNYIRGQEGLIKYKVDSKERRIDDGSIIKNMVPGNDVVL
HCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHCCCCCHHHHHCCCCCCEEE
NINKDIQDLAKNALGKRYGSVVVLKPSTGAVLALHNYPYYSMKDVYNKDNKEDYSFLNKA
EECCCHHHHHHHHHHHHHCCEEEEECCCCCEEEEECCCCCHHHHHHCCCCCHHHHHHHHH
IQSVYPPASIFKLVVAAAILEERVIDKDRKIYCPGYFKVGNRIFHCWKPGGHGYVNLEEA
HHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHCCEEEEEECCCCCCEEEHHHH
IAHSSNVYFYTLGLKYLGVDRIRKYAKEFGFGEKTGIDLPNEVAGLLPSPEWKEKTFNQP
HHCCCCEEEEEECHHHHCHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCHHHHCCCCC
WVGGDTVNFSIGQGFLNATPMQIVNMVAMIANEGVVYKPRIVNKILKGGTNKVVLENKPE
CCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEECHHHHHHHHHCCCCEEEECCCHH
ILRKTNLISKNTFKLLKKYMRSVITYGTARYAVLTKAVEVGGKTGTGQTGIDGFENSSFI
HHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEE
GLAPYNGSADNQIIVFSLVEAKSNVDWWPAKSTDLIMQGIFANQSYEDILKGYRPWYIR
EEECCCCCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]