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The map label for this gene is rpoD

Identifier: 15595057

GI number: 15595057

Start: 748123

End: 750018

Strand: Direct

Name: rpoD

Synonym: BB0712

Alternate gene names: 15595057

Gene position: 748123-750018 (Clockwise)

Preceding gene: 15595056

Following gene: 15595058

Centisome position: 82.15

GC content: 31.91

Gene sequence:

>1896_bases
TTGTCGGATTTGGAAAAGAAATATTCGAAGCTGATAGAGGGTATTATTACTCATTTGGGAGATAGAAAATCTCTTAGTTT
TAGTGAATTATCAAATTTGCTTCCCGATGATATATTAGAACCAGAGATTCTTGATTGTATTTGTTCGGTACTTGAGGATA
GGGGAATAAGGTTGGTTAATAAAATTTCGGAATTAGATCTGGTTGTCAGTGAAGATGGAAATGATGAAGAGGAAGAGGTT
GAGATTGAATCTGATAGAAATTTTATGATTTTAGATGATGGTTTTCAAAGTGATGAGGAAGATATTGACATTGATGTTAA
GCTGGATGATTGTGATGAAGAAGATATTTCTGTTAAGGATGATTTAGGTTCAGGGTATATTAAAGGCAATGTTTTAAAAG
ATAGTCACTCAGAAGATCCAATCAAGCTTTATTTAAAGGAAATAGGAAAAGAGTTTTTATTAACGGGAAATCAAGAAGTT
GAACTTGCAAAGCAAATGGATTCTGGAGAGAGTATAATTGAGAATATTCTTAAGAATGAGGGACTTGTTATAGAAAATTA
TTATAATCTTGTGAATACTATTTACTCAAGAATGGAAAGAGAAGAGTTTTTTAAAAGAGAAAAAGATAAGGATAAAGAGA
GTAGTCCGGATTATTATAATAAAAAAAAAAGAATTGCCTCTTTTTACAAAATTCCTTTAAAGCCAATTCAAGATCGTTTA
ATAAGTTATGTAGATAATAAGCATAGGGTGTATGATCTTGGGGGGGATATTTTTGAAAAGAATTTAAAAAAGGAAAGATT
GGCCCTAAAAGAGCTTTTGAGAGACATTCCTTTGTATCAAGAGGAATTAAGGATTTTCTCAGATGATTATATTGACTCTG
CTAACAAAATAAAAGATTTACAAAGACAACAAAGAATAATTCTAAGCAGGCTGAAAATTGAAAAAATAAGAGATTTGAGG
GTGCTTGGAAGAGATTTGACTATTGCTGAAAAAAAAATAGAGATAGAAAAATCTCTCAAGCTTAAAGAAGATGCTATTAA
AGAGCAGATTACAGAGGCTCAGCTTGCCCAAAAAGAACTTGAGAGAATTGAGATGTATTATGAATATCCAACTGATAAAA
TAATAAGCATGTCAGAAGAGATTGCTAAAGGGAAGCAAATGATGCAGCATGCTAAAGATCAGTTGATTAAGGCTAATTTA
AGGCTTGTTGTAAGCATTGCTAAAAAATATGCAAATAGAGGTCTTCATTTTTTTGATCTTGTTCAAGAAGGCAATATTGG
ATTGATTAAGGCTGTTGAAAAGTTCGAATATAAGAGAGGTTTTAAGTTTTCAACCTATGCTACTTGGTGGATTAGACAAG
CCATAACAAGATCTATTTCTGATCAAGCTCGCACAATTAGAGTTCCTGTGCACATGATTGAGCAAATAAATAGGCTTAAT
AGAGAAACTAGATATTTAATTCAAGTTTTAGGCAAAGATCCCACAGATGAAGAGCTTTCAGACAGGCTTGGATGGGAACT
TAAAAAGGTTAAAACTGTAAAGAGTGTTTCAAGAGAACCTGTTTCTCTTGAAACACCAATTGGAGAAGAGGAAGATTCTG
TTCTTAGCGATTTTATTGAGGATAAGGCAATAAAAAATCCTGCAAATCACACATCTTTTGTAGTTTTGCAAGATCAAATA
AGAGCAATTCTTGGAACTCTTCCTGAAAGAGAACAAGAAGTTGTAAAAATGAGATTTGGACTTGAAGATGGCTATTCTTT
AACTCTTGAAGAGGTTGGACTTCATTTTAATGTTACAAGAGAAAGAATTAGGCAAATTGAATCTAAAGCATTAAGGCGGC
TTAAAAATCCCAAAAAAACTCAAAAATTAAAAGATTATCTTGAAGATTTAAATTGA

Upstream 100 bases:

>100_bases
TTTGGTAAATGCTAAGATTCAAATAAATGAGTTAATGTTTTTAAATATGCAGAGAAAAAATTTAAAAATCTACATAGATG
ATGTTCCAGGGAGTTAGGTT

Downstream 100 bases:

>100_bases
ACAGGAGGATTGATGGAAAACAATATTGATACATTAAAAAAACTTGAAGTTATATATAAATCTAAGTTTGAGCTTGAAGA
AAGGCGAAAAAGTATTCCCA

Product: RNA polymerase sigma factor RpoD

Products: NA

Alternate protein names: Sigma-70

Number of amino acids: Translated: 631; Mature: 630

Protein sequence:

>631_residues
MSDLEKKYSKLIEGIITHLGDRKSLSFSELSNLLPDDILEPEILDCICSVLEDRGIRLVNKISELDLVVSEDGNDEEEEV
EIESDRNFMILDDGFQSDEEDIDIDVKLDDCDEEDISVKDDLGSGYIKGNVLKDSHSEDPIKLYLKEIGKEFLLTGNQEV
ELAKQMDSGESIIENILKNEGLVIENYYNLVNTIYSRMEREEFFKREKDKDKESSPDYYNKKKRIASFYKIPLKPIQDRL
ISYVDNKHRVYDLGGDIFEKNLKKERLALKELLRDIPLYQEELRIFSDDYIDSANKIKDLQRQQRIILSRLKIEKIRDLR
VLGRDLTIAEKKIEIEKSLKLKEDAIKEQITEAQLAQKELERIEMYYEYPTDKIISMSEEIAKGKQMMQHAKDQLIKANL
RLVVSIAKKYANRGLHFFDLVQEGNIGLIKAVEKFEYKRGFKFSTYATWWIRQAITRSISDQARTIRVPVHMIEQINRLN
RETRYLIQVLGKDPTDEELSDRLGWELKKVKTVKSVSREPVSLETPIGEEEDSVLSDFIEDKAIKNPANHTSFVVLQDQI
RAILGTLPEREQEVVKMRFGLEDGYSLTLEEVGLHFNVTRERIRQIESKALRRLKNPKKTQKLKDYLEDLN

Sequences:

>Translated_631_residues
MSDLEKKYSKLIEGIITHLGDRKSLSFSELSNLLPDDILEPEILDCICSVLEDRGIRLVNKISELDLVVSEDGNDEEEEV
EIESDRNFMILDDGFQSDEEDIDIDVKLDDCDEEDISVKDDLGSGYIKGNVLKDSHSEDPIKLYLKEIGKEFLLTGNQEV
ELAKQMDSGESIIENILKNEGLVIENYYNLVNTIYSRMEREEFFKREKDKDKESSPDYYNKKKRIASFYKIPLKPIQDRL
ISYVDNKHRVYDLGGDIFEKNLKKERLALKELLRDIPLYQEELRIFSDDYIDSANKIKDLQRQQRIILSRLKIEKIRDLR
VLGRDLTIAEKKIEIEKSLKLKEDAIKEQITEAQLAQKELERIEMYYEYPTDKIISMSEEIAKGKQMMQHAKDQLIKANL
RLVVSIAKKYANRGLHFFDLVQEGNIGLIKAVEKFEYKRGFKFSTYATWWIRQAITRSISDQARTIRVPVHMIEQINRLN
RETRYLIQVLGKDPTDEELSDRLGWELKKVKTVKSVSREPVSLETPIGEEEDSVLSDFIEDKAIKNPANHTSFVVLQDQI
RAILGTLPEREQEVVKMRFGLEDGYSLTLEEVGLHFNVTRERIRQIESKALRRLKNPKKTQKLKDYLEDLN
>Mature_630_residues
SDLEKKYSKLIEGIITHLGDRKSLSFSELSNLLPDDILEPEILDCICSVLEDRGIRLVNKISELDLVVSEDGNDEEEEVE
IESDRNFMILDDGFQSDEEDIDIDVKLDDCDEEDISVKDDLGSGYIKGNVLKDSHSEDPIKLYLKEIGKEFLLTGNQEVE
LAKQMDSGESIIENILKNEGLVIENYYNLVNTIYSRMEREEFFKREKDKDKESSPDYYNKKKRIASFYKIPLKPIQDRLI
SYVDNKHRVYDLGGDIFEKNLKKERLALKELLRDIPLYQEELRIFSDDYIDSANKIKDLQRQQRIILSRLKIEKIRDLRV
LGRDLTIAEKKIEIEKSLKLKEDAIKEQITEAQLAQKELERIEMYYEYPTDKIISMSEEIAKGKQMMQHAKDQLIKANLR
LVVSIAKKYANRGLHFFDLVQEGNIGLIKAVEKFEYKRGFKFSTYATWWIRQAITRSISDQARTIRVPVHMIEQINRLNR
ETRYLIQVLGKDPTDEELSDRLGWELKKVKTVKSVSREPVSLETPIGEEEDSVLSDFIEDKAIKNPANHTSFVVLQDQIR
AILGTLPEREQEVVKMRFGLEDGYSLTLEEVGLHFNVTRERIRQIESKALRRLKNPKKTQKLKDYLEDLN

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family

Homologues:

Organism=Escherichia coli, GI1789448, Length=278, Percent_Identity=51.7985611510791, Blast_Score=305, Evalue=5e-84,
Organism=Escherichia coli, GI1789098, Length=312, Percent_Identity=36.5384615384615, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI1789871, Length=262, Percent_Identity=29.0076335877863, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1788231, Length=210, Percent_Identity=27.6190476190476, Blast_Score=69, Evalue=8e-13,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): RPOD_BORBU (P52323)

Other databases:

- EMBL:   U17591
- EMBL:   AE000783
- EMBL:   U68006
- PIR:   G70188
- RefSeq:   NP_212846.1
- ProteinModelPortal:   P52323
- SMR:   P52323
- EnsemblBacteria:   EBBORT00000008359
- GeneID:   1195566
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0712
- NMPDR:   fig|224326.1.peg.1096
- TIGR:   BB_0712
- GeneTree:   EBGT00050000006769
- HOGENOM:   HBG745096
- OMA:   ESIIENI
- ProtClustDB:   PRK05658
- BioCyc:   BBUR224326:BB_0712-MONOMER
- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00046
- TIGRFAMs:   TIGR02393
- TIGRFAMs:   TIGR02937

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4; SSF88946 Sigma_r2; SSF88659 Sigma_r3_r4

EC number: NA

Molecular weight: Translated: 73644; Mature: 73513

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00715 SIGMA70_1; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLEKKYSKLIEGIITHLGDRKSLSFSELSNLLPDDILEPEILDCICSVLEDRGIRLVN
CCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHH
KISELDLVVSEDGNDEEEEVEIESDRNFMILDDGFQSDEEDIDIDVKLDDCDEEDISVKD
HHHHHHEEEECCCCCCHHHEEEECCCCEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCC
DLGSGYIKGNVLKDSHSEDPIKLYLKEIGKEFLLTGNQEVELAKQMDSGESIIENILKNE
CCCCCCEECCEECCCCCCCHHHHHHHHHHHHHEECCCCHHHHHHHCCCCHHHHHHHHHCC
GLVIENYYNLVNTIYSRMEREEFFKREKDKDKESSPDYYNKKKRIASFYKIPLKPIQDRL
CEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHH
ISYVDNKHRVYDLGGDIFEKNLKKERLALKELLRDIPLYQEELRIFSDDYIDSANKIKDL
HHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHH
QRQQRIILSRLKIEKIRDLRVLGRDLTIAEKKIEIEKSLKLKEDAIKEQITEAQLAQKEL
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERIEMYYEYPTDKIISMSEEIAKGKQMMQHAKDQLIKANLRLVVSIAKKYANRGLHFFDL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
VQEGNIGLIKAVEKFEYKRGFKFSTYATWWIRQAITRSISDQARTIRVPVHMIEQINRLN
HHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHH
RETRYLIQVLGKDPTDEELSDRLGWELKKVKTVKSVSREPVSLETPIGEEEDSVLSDFIE
HHHHHHHHHHCCCCCHHHHHHHHCHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHH
DKAIKNPANHTSFVVLQDQIRAILGTLPEREQEVVKMRFGLEDGYSLTLEEVGLHFNVTR
HHHHCCCCCCCEEEEEHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEHHHHCCCCHHHH
ERIRQIESKALRRLKNPKKTQKLKDYLEDLN
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDLEKKYSKLIEGIITHLGDRKSLSFSELSNLLPDDILEPEILDCICSVLEDRGIRLVN
CHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHH
KISELDLVVSEDGNDEEEEVEIESDRNFMILDDGFQSDEEDIDIDVKLDDCDEEDISVKD
HHHHHHEEEECCCCCCHHHEEEECCCCEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCC
DLGSGYIKGNVLKDSHSEDPIKLYLKEIGKEFLLTGNQEVELAKQMDSGESIIENILKNE
CCCCCCEECCEECCCCCCCHHHHHHHHHHHHHEECCCCHHHHHHHCCCCHHHHHHHHHCC
GLVIENYYNLVNTIYSRMEREEFFKREKDKDKESSPDYYNKKKRIASFYKIPLKPIQDRL
CEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHH
ISYVDNKHRVYDLGGDIFEKNLKKERLALKELLRDIPLYQEELRIFSDDYIDSANKIKDL
HHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHH
QRQQRIILSRLKIEKIRDLRVLGRDLTIAEKKIEIEKSLKLKEDAIKEQITEAQLAQKEL
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERIEMYYEYPTDKIISMSEEIAKGKQMMQHAKDQLIKANLRLVVSIAKKYANRGLHFFDL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
VQEGNIGLIKAVEKFEYKRGFKFSTYATWWIRQAITRSISDQARTIRVPVHMIEQINRLN
HHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHH
RETRYLIQVLGKDPTDEELSDRLGWELKKVKTVKSVSREPVSLETPIGEEEDSVLSDFIE
HHHHHHHHHHCCCCCHHHHHHHHCHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHH
DKAIKNPANHTSFVVLQDQIRAILGTLPEREQEVVKMRFGLEDGYSLTLEEVGLHFNVTR
HHHHCCCCCCCEEEEEHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEHHHHCCCCHHHH
ERIRQIESKALRRLKNPKKTQKLKDYLEDLN
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685; 9084142